FastQCFastQC Report
Wed 20 Mar 2013
8016-8-27.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-27.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4804924
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10699 0.2226674136781352 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 10695 0.22258416574330833 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10683 0.22233442193882774 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10639 0.22141869465573233 No Hit
TGAGACCGCACCAGCGACACA 9402 0.19567427081052688 No Hit
TGTGTCGCTGGTGCGGTCTCA 9287 0.19328089268425475 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8763 0.18237541322193648 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8762 0.18235460123822977 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8552 0.1779840846598198 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8505 0.17700592142560423 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8319 0.1731348924561554 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 8260 0.17190698541745925 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 8119 0.16897249571481257 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 8090 0.16836894818731785 No Hit
TAAACATGTCTGCAGAAGAGCAGCTGTTGCCCTTGGCCGAAAACGAGCTG 7936 0.16516390269648387 No Hit
ATCCCCTCTTGCCATCTGTTTTTCAGAGGACCTACTAACTATGAAGAGCC 7886 0.16412330351114815 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7859 0.16356137995106684 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7840 0.1631659522606393 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 7800 0.16233347291237074 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 7763 0.16156342951522232 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 7717 0.16060607826471346 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 7689 0.16002334272092544 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 7656 0.15933654725860388 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7647 0.15914923940524345 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 7637 0.1589411195681763 No Hit
* 7563 0.15740103277387946 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 7533 0.15677667326267802 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 7516 0.1564228695396639 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7486 0.15579851002846246 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 7471 0.15548633027286177 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7389 0.1537797476089112 No Hit
AGAAGAGGGCTCCTTTTGTGCATATCAAACCTTGTTCCAGAATGGAGTGG 6890 0.1433945677392608 No Hit
GACCCCCTCCACTAACTCCCGAGGACGTTGGCTTTGCATCTGGTTTTTCT 6878 0.14314482393478023 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 6849 0.14254127640728553 No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA 6803 0.14158392515677667 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6738 0.14023114621584026 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6655 0.13850375156818298 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6621 0.13779614412215468 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 6584 0.13702610072500626 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 6526 0.13581900567001684 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6496 0.1351946461588154 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6487 0.135007338305455 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 6464 0.13452866268020058 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 6454 0.1343205428431334 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 6440 0.1340291750712394 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6433 0.1338834911852924 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6401 0.13321750770667756 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6366 0.13248908827694256 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 6200 0.129034298981628 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6116 0.12728609235026403 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6107 0.1270987844969036 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6094 0.1268282287087163 No Hit
GATCCAAAACTTCTCTCCGACACTGAATACCTTCTGCTTCTCCTATCATT 6061 0.12614143324639474 No Hit
TGATCCCTTTTTCCTTTTCTCTTTCCTTGACTCCCGCTTATTCTCCTTTT 5994 0.12474703033804489 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5986 0.12458053446839118 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5982 0.12449728653356432 No Hit
GACTGAAAGAGGGAGAAATAAAGACAAGGCCCCCGAGGAGCTGTCCAAAG 5955 0.12393536297348304 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 5756 0.11979377821584691 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 5743 0.11952322242765963 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 5592 0.11638061288794578 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 5560 0.11571462940933093 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 5556 0.11563138147450407 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 5535 0.11519432981666308 No Hit
GACCACACACCTTGGCTTATGCCTCCAACAATCCCCTTAGCATCACCATT 5466 0.1137583029408998 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 5425 0.11290501160892451 No Hit
TGACCTAGAATGCTTTAAAGAAGTCCACCTAAATGTCGGTTCTCGCAAAA 5406 0.11250958391849694 No Hit
TGATTTTTCTCTGCAGATTCCCCAGACTGAGTCAGGGAATGAGAAGGAAA 5393 0.11223902813030966 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 5353 0.11140654878204108 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5216 0.10855530701422125 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 5192 0.1080558194052601 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 5186 0.10793094750301982 No Hit
CAGAGAAAAATCAGAGACCAAGGAAGCAGACTAGTGCTCCGGCAGAGCCA 5185 0.10791013551931311 No Hit
CCTTGTCTTTATTTCTCCCTCTTTCAGTCTGAGAGCCTGGGGTAAACCAA 5185 0.10791013551931311 No Hit
TGAGGAGCGGAGCGATACCCCTGAAGTTCATCCTCCACTGCCCATTTCCC 5015 0.1043720982891717 No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA 5002 0.10410154250098441 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 4982 0.10368530282685012 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 4973 0.1034979949734897 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 4912 0.10222846396738014 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 4911 0.10220765198367342 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 4888 0.10172897635841897 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 4853 0.101000556928684 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 4825 0.100417821384896 No Hit
CATGCTTTACTCAACTGAAATAATCAAGCCCCTATTGCTGTGGGGGAAGC 4805 0.10000158171076172 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2728430 3.441788 6.579879 2
TTTTT 2524525 3.420099 4.6214986 8
GGGGG 654700 3.0016925 8.398763 4
GGAGG 788985 2.7944872 5.661086 4
CCTCC 785365 2.5855331 10.44951 6
CCCCA 795805 2.5827813 6.1911416 1
CCAGC 777045 2.5775986 8.050769 5
CCTGG 734465 2.5259562 5.5128508 9
CAGCC 747125 2.4783485 5.8210907 6
TGGGG 673690 2.4204216 10.333483 3
AGCAG 843290 2.2575204 5.014798 5
GCTGG 624750 2.1960845 7.670916 7
GAGGG 614025 2.1748006 6.722495 9
TTTTC 1273780 2.1545587 5.125467 9
CCCTC 635595 2.092469 5.8371353 5
GGGGA 589365 2.0874577 5.825833 7
CCACC 638315 2.0716486 5.241562 1
CTCCA 781740 2.0320692 5.729487 6
TGGAG 721980 2.0038517 5.0942044 3
CTCCT 751715 1.9821062 5.8478603 4
AAGAA 1203070 1.964503 5.2411427 2
TCCAG 731550 1.943606 6.4504733 7
TGGCT 696875 1.9195733 7.0354304 3
CCCAC 584100 1.895694 6.3626738 9
GCCAG 555505 1.8834112 7.0009956 4
TGTTT 1081335 1.8694432 5.766106 3
GGCTC 542315 1.8651183 7.5761285 4
TCCTC 704125 1.8566219 6.6673636 5
CACCA 720125 1.8453829 5.934986 8
TGCAG 678025 1.8411875 5.885258 3
TGGTG 649840 1.8295504 5.39873 9
GCCCA 536350 1.7791697 5.829205 4
AAACA 1096485 1.7517673 6.0132537 2
CCAGA 662285 1.7346491 5.3665657 8
AGGGG 489580 1.7340318 7.712701 6
GAAAG 770015 1.6276144 5.7191997 5
GGCCC 373625 1.6043367 5.1432405 1
TGCCC 466550 1.5698742 11.03764 3
GCTCC 457530 1.5395231 7.28049 2
TAAAA 1178810 1.5083866 8.448979 1
ATGGA 695330 1.4908738 10.831802 2
GATGG 534365 1.4831272 5.816144 1
CATGG 542135 1.4721761 42.834423 1
GGGGT 374860 1.3467903 5.3489027 4
GCAGT 489670 1.3297065 5.699715 4
TGCTC 481480 1.2975975 5.400543 1
ATGTG 587135 1.2769837 7.981789 2
TGGGT 444515 1.2514813 6.24867 3
ATGGG 447140 1.2410349 14.970621 2
ATGGC 453595 1.2317443 11.110418 2
ATGAA 743155 1.2309453 9.024438 2
AGACA 593780 1.2279757 5.0587497 2
TGAGA 572195 1.2268568 5.830538 1
ATGCC 452175 1.2013535 11.111388 2
CATGT 521140 1.1089545 32.982025 1
GAGAC 413165 1.106059 6.203333 2
ATGTT 612090 1.0432053 8.062844 2
CATGA 493050 1.0343149 29.660484 1
TAAAG 606725 1.0049658 6.7016044 1
GTGTC 364490 1.004004 5.0678935 2
ATGCT 465990 0.9915987 7.766725 2
ATGGT 452070 0.9832255 7.628596 2
AGACT 468050 0.98187 5.8118773 1
AGACC 374625 0.9812133 5.663062 3
ATGAC 467480 0.9806744 7.33163 2
ATGAT 571590 0.9603765 6.5726266 2
ATGTA 555770 0.933796 9.605245 2
GACTC 349535 0.92865604 5.1736927 2
CATGC 341850 0.9082383 31.421373 1
GCGCC 208015 0.8932114 5.533843 8
CGCTG 241840 0.8317309 5.7578626 6
TAAGA 492605 0.81593984 5.105947 1
TAACT 458875 0.7543332 5.4818535 1
TAAGT 445450 0.74843806 7.1097617 1
ATGCA 350555 0.7353904 8.917824 2
TAAAC 447910 0.7258753 6.589949 1
TAAGG 294400 0.63123 5.1892385 1
TGCCG 160805 0.55303705 8.008623 5
CCGTC 131605 0.44283202 5.068868 7
GCCGT 118680 0.40816167 5.1439257 6
GTCGC 97530 0.3354231 5.1266313 4
Produced by FastQC (version 0.10.0)