FastQCFastQC Report
Wed 20 Mar 2013
8016-8-28.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-28.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5037606
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 15058 0.29891182438642483 No Hit
TGAGACCGCACCAGCGACACA 12954 0.25714595385188915 No Hit
TGTGTCGCTGGTGCGGTCTCA 12816 0.2544065574004795 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11795 0.23413899379983272 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11746 0.23316630955259304 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11700 0.23225317740212312 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 11637 0.23100258336995788 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 11458 0.22744930826269463 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 11405 0.2263972212197619 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 9090 0.18044285321241876 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 8911 0.1768895781051555 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 8797 0.17462659842790407 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 8758 0.17385242116989696 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 8669 0.17208570896572697 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 8622 0.17115272611633384 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8545 0.16962422229924293 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 8529 0.1693066111164708 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8424 0.16722228772952866 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 8371 0.16617020068659596 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 8369 0.16613049928874946 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 8347 0.16569378391243778 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 8282 0.16440348848242597 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 8276 0.16428438428888642 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8200 0.16277573117071878 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 8156 0.16190230041809542 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7455 0.1479869604728913 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7429 0.14747084230088658 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 7225 0.14342129972054185 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 7202 0.14296473364530693 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6913 0.13722788165648525 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6776 0.13450833590399885 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6736 0.1337143079470685 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6709 0.13317833907614052 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6684 0.1326820716030591 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6682 0.13264237020521255 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6651 0.13202699853859154 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6432 0.12767969547439795 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6339 0.12583358047453494 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6337 0.1257938790766884 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6301 0.1250792539154511 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6141 0.12190314208772976 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 6098 0.12104956203402965 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 6042 0.11993792289432718 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5991 0.11892553724924101 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 5975 0.11860792606646886 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5961 0.11833001628154326 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 5943 0.11797270370092461 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5898 0.11707942224937799 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 5716 0.11346659504534494 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 5698 0.1131092824647263 No Hit
GTATCTTAATGATGTATATAATTGCCTTCAATCCCCTTCTCACCCCACCC 5648 0.11211674751856339 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 5648 0.11211674751856339 No Hit
CATGCTTTACTCAACTGAAATAATCAAGCCCCTATTGCTGTGGGGGAAGC 5620 0.11156092794871214 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 5601 0.11118376466917024 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 5573 0.110627945099319 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5568 0.11052869160470272 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 5544 0.1100522748305445 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 5537 0.10991331993808169 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 5494 0.10905973988438158 No Hit
CATGACTATAAATATCAAGCAAATATGATTTTACTCACAAGTTCTGGAAT 5407 0.1073327290780581 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 5390 0.10699526719636272 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 5365 0.10649899972328125 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATCTGGGTTTTTTTC 5354 0.10628064203512541 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 5343 0.10606228434696957 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5331 0.10582407595989046 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 5294 0.10508960009972992 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5271 0.10463303402449496 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 5222 0.10366034977725531 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 5203 0.10328318649771341 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 5196 0.10314423160525057 No Hit
TTAGCTTTGAAGTGGATCCTACCAGTTTTATCATCTTTTCACATAAAAGT 5193 0.10308467950848081 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 5186 0.102945724616018 No Hit
AGTTCTAACATTTTGTTTATGGCTTTTTGGGGGGTTTTCATTGCAGGATG 5145 0.1021318459601644 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 5145 0.1021318459601644 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 5122 0.10167527988492947 No Hit
TAAGCAATGCCACAGGGGCATGATATCCTGGCTTCTTCTGACCAAGCAAA 5121 0.10165542918600622 No Hit
AGAACAGTTGCAAATAGACTACATAATATACGTGGGCAAAAAGGCAATTA 5112 0.10147677289569687 No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC 5091 0.10105990821830846 No Hit
TAATTGCCTTTTTGCCCACGTATATTATGTAGTCTATTTGCAACTGTTCT 5073 0.1007025956376898 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2923075 3.4465344 6.5165195 2
TTTTT 2636635 3.3854768 4.370943 60-64
GGGGG 735035 3.2508428 8.896006 4
CCAGG 812565 2.6676698 5.57808 5
CCAGC 826105 2.6669617 8.128776 5
CCTGG 775845 2.5909224 5.9193187 9
CCTCC 796020 2.5705054 9.137746 6
CCCCA 803125 2.549601 5.6842256 1
CAGCC 774005 2.4987643 6.5188336 6
TGGGG 697490 2.4088087 11.491238 3
GCTGG 660755 2.243946 9.36835 7
GGCAG 660360 2.2046885 5.11854 8
GAGGG 625580 2.1239376 7.045177 9
GGGGA 624810 2.1213233 5.811094 7
CCACC 642765 2.0405219 5.255366 1
CCCTC 628240 2.0287106 6.077909 5
CTCCT 779040 1.9976671 5.0303535 4
CTCCA 787990 1.9864544 5.640957 6
TCCAG 772445 1.9802407 6.466771 7
CACAC 784825 1.9450253 5.0434146 8
CCCAC 608515 1.931792 7.0345592 9
GCCAG 586105 1.9241966 7.662501 4
TGGCT 713750 1.892755 7.08484 3
CCCTG 573830 1.8843875 5.180157 1
CACCA 756980 1.8760172 5.9302444 8
TGCAG 709710 1.8502215 6.314467 3
TGGTG 685535 1.8487167 6.7414584 9
TCCTC 718325 1.8419776 5.7225575 5
TGTTT 1114715 1.8329782 5.8442774 3
GGCTC 548245 1.8308557 7.6282125 4
GCCCA 563790 1.8201153 5.8739886 4
AGGGG 518655 1.7609112 7.4867563 6
AAACA 1142660 1.7258232 5.045469 2
CCAGA 683705 1.7231125 5.0632267 8
GGCCC 391400 1.6460061 5.2789783 1
GAAAG 810460 1.6215576 5.3846684 5
GCTCC 471210 1.547396 7.58317 2
TGCCC 470045 1.5435702 11.828621 3
CTGGT 575460 1.5260313 5.74167 8
AGCAA 769855 1.5146674 5.207141 8
TAAAA 1252215 1.5018516 8.694061 1
ATGGA 733510 1.4928366 10.018152 2
GATGG 560880 1.4869804 6.175875 1
CATGG 555040 1.4469951 41.72582 1
GCAGT 516945 1.3476814 5.706681 4
GGGGT 388090 1.340284 5.4362493 4
TGCTC 503675 1.3134277 5.6690793 1
TGGGT 479570 1.2932804 5.901452 3
ATGTG 620160 1.2838533 7.76799 2
ATGAA 799945 1.2497907 9.082922 2
TGAGA 607910 1.237216 6.6438174 1
ATGGC 473490 1.2343935 11.075848 2
ATGGG 457465 1.2128111 14.89973 2
AGACA 612275 1.2046332 5.2236614 2
ATGCC 465905 1.1943944 11.613708 2
GAGAC 431220 1.1051883 7.0389786 2
AACAC 569565 1.1019431 5.1815825 2
GCACC 340170 1.0981902 5.2840652 8
CATGT 534290 1.0876676 32.399868 1
CATGA 533300 1.0672972 30.599703 1
ATGTT 652340 1.0545375 8.244748 2
TAAAG 655370 1.0239146 6.8423553 1
GTGTC 386000 1.0236125 6.0387425 2
ATGCT 494370 1.0064015 7.9684157 2
AGACT 496310 0.9932688 5.4396787 1
ATGGT 478755 0.99111706 7.7218666 2
AGACC 388875 0.98006517 6.7625604 3
CACTC 387835 0.9776984 5.3879776 1
ATGAT 612625 0.9735927 6.861667 2
ATGAC 479690 0.9600071 7.495562 2
ATGTA 584330 0.9286259 9.084222 2
CATGC 357150 0.9155899 33.115685 1
GCGCC 215800 0.9075322 5.943254 8
CGCTG 254600 0.85023284 7.1762223 6
TAACT 485275 0.7583647 5.956154 1
TAAGT 460355 0.731603 6.5579505 1
ATGCA 364600 0.7296766 9.257749 2
TAAAC 454625 0.6984543 5.44607 1
TAAGG 317660 0.6465004 5.728309 1
TGCCG 163415 0.5457218 6.8628225 5
CCGCA 165030 0.5327757 5.110045 6
GACCG 158385 0.5199817 6.0665274 4
TCGCT 163475 0.42629194 5.248662 5
ACCGC 127350 0.41113123 6.0928307 5
GTCGC 105305 0.3516644 6.6845794 4
TGTCG 128140 0.33980754 5.4285474 3
Produced by FastQC (version 0.10.0)