FastQCFastQC Report
Wed 20 Mar 2013
8016-8-30.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-30.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6197654
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 30776 0.4965749943446343 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 30616 0.4939933723308852 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 16466 0.2656811754899515 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 15731 0.2538218493642917 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 15720 0.25364436285084646 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 13999 0.22587579106545802 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 13953 0.22513357473650514 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 13930 0.22476246657202872 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 13626 0.2198573847459055 No Hit
CGAGACCGCGCCCCAGGCCCAGCACTCACAAATTCCTGGTCGTGGTTATT 13105 0.21145097806363503 No Hit
GGCACAAGGGTACCTACGGGCTGCTGCGGCGGCGAGAGGACTGGCCCTCC 12985 0.20951476155332324 No Hit
ATACTTGTAATTCCCCTAACCATAAGATTTACTGCTGCTGTGGATATCTC 12471 0.20122130083415432 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 11649 0.18795821773851848 No Hit
CTGAGGAAATAAGATGTAGGGCATTTCTGATGTGACTCAGTGGGAAAACT 11644 0.18787754205058882 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 11635 0.18773232581231544 No Hit
TGCTCTGTGGTCCGAGGCGAGCCGTGAAGTTGCGTGTGCGTGGCAGTGTG 11544 0.18626402829199565 No Hit
CCGCGGCGCAGATCGAAACAGATCGGGCGGCTCGGGTTACACACGCACGC 11468 0.18503775783546483 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 11271 0.1818591357310363 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 11192 0.18058445986174768 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 9425 0.15207367174740635 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 9382 0.1513798608312113 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 8906 0.1436995353403078 No Hit
CGGACGGGTCGGTGGCGTAGACGCGGAGCGCGCAGCTCACACCTGGCGGC 8846 0.1427314270851519 No Hit
GGCTCCGCGGAGGCGCCACGCACAGTGTCCAAAGCATCCTTGCTTCCTCC 8805 0.14206988644412868 No Hit
AACCTGACAGAAAAACAAAACTCTTCATATTGGTCAATTTCATAATCTTC 8715 0.14061772406139483 No Hit
TAACTAACTCATAAATGCCTATTTTTTCTTACAGAAAAGGGATTTATAAA 8681 0.14006912938347316 No Hit
CATGCCCACAGAGACTTGCACAACATGCAGAATGGCAGCACATTGGTAAG 8669 0.13987550773244198 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 8660 0.1397302914941686 No Hit
ATAGGACCTTCAGTCGGCAACGTGTTCTCAGTGGGTTCCAGCTTGTGTGG 8548 0.13792315608454425 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 8409 0.13568037196009974 No Hit
ACTACTGTACAACCCGACTTCATAATGGTGCTTTCAAACAGCGAGATGAG 8325 0.13432502040288147 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 8285 0.13367961489944422 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 8272 0.1334698581108271 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 8219 0.13261469581877272 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 8149 0.1314852361877575 No Hit
AAGAAATGTGTACTGCTAAATGTGAACTGCTACTTTTTTCTAAGTAGTTT 7957 0.1283872897712586 No Hit
CACCGTGAGAACGCGCCATCTGCAAGCTGTCTCCCTTTCTCCATCCTTGG 7951 0.12829047894574303 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 7940 0.12811299243229776 No Hit
CTGGGGTCTCAACGCACACCCGAGGTCTTCCGGGGATTCTGATACCTACG 7928 0.1279193707812666 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7913 0.1276773437174776 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7900 0.12746758692886048 No Hit
CTGGAGGACCGAGGTATACAAGAAGTGTTGCCATTTATCAAAAGGTCATC 7784 0.12559591096889242 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTATCTGCATTTCCC 7430 0.1198840722634726 No Hit
AGAACAGTTGCAAATAGACTACATAATATACGTGGGCAAAAAGGCAATTA 7425 0.11980339657554294 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAAAAGTAATTCTGGCCTGGCAACCTA 7376 0.11901277483383227 No Hit
TAATTGCCTTTTTGCCCACGTATATTATGTAGTCTATTTGCAACTGTTCT 7364 0.1188191531828011 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7244 0.1168829366724893 No Hit
TAAAAATTTACAGATTGTGCTGAGCTTGACAAATAAGTGTATCCTTATGT 7179 0.11583415272940374 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7145 0.11528555805148206 No Hit
TGCTCTAAAATTTCATTTATCATACTGAAATATACTCACCAGAATATGGG 7107 0.11467242282321664 No Hit
TAACCAAAGTCTTGCCACAAGACAAAGAATACTATAAAGTAAAAGAACCT 7028 0.11339774695392806 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 7007 0.11305890906462349 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 6983 0.11267166576256113 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 6959 0.11228442246049876 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 6946 0.11207466567188164 No Hit
ACCCTATATATTCCCTTTATTATCTATGAAAACGTCTAGATGATTTCCTA 6945 0.11205853053429572 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 6933 0.11186490888326454 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 6909 0.11147766558120217 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 6901 0.11134858448051473 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 6896 0.11126790879258508 No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG 6875 0.11092907090328051 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 6866 0.11078385466500712 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 6859 0.1106709087019056 No Hit
TAATTATGGAATATTTACCATATGGAAGTTTACGAGACTATCTTCAAAAA 6857 0.11063863842673373 No Hit
ATCCCCTCTTGCCATCTGTTTTTCAGAGGACCTACTAACTATGAAGAGCC 6852 0.11055796273880408 No Hit
TAAACATGTCTGCAGAAGAGCAGCTGTTGCCCTTGGCCGAAAACGAGCTG 6818 0.11000936806088239 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 6815 0.10996096264812459 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6812 0.1099125572353668 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 6772 0.10926715173192954 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6700 0.10810542182574244 No Hit
GGCACCCTTTTCTCGCCGCAAGAAGCCCATTCCTATGGAAGTCTAGCAAA 6645 0.1072179892585162 No Hit
CAGGATGGAAGGCACAGCAGAGCCATGAGTCCTGGGGCAGAGAGTGCTGG 6599 0.10647577292956334 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6580 0.10616920531543064 No Hit
AAGAAGTCCACCTAAATGTCGGTTCTCGCAAAATAGATTTTCGCTTCCCT 6523 0.10524950247303252 No Hit
ATCATGTCCCCAGAAAATCCAGTTACCTCTTGGTCAGTCATCCGGAAGCA 6499 0.10486225917097018 No Hit
TTAGCTTTGAAGTGGATCCTACCAGTTTTATCATCTTTTCACATAAAAGT 6491 0.1047331780702827 No Hit
TAATAGGGTGGTTCTCTTCCCAAAGTGGAAGCCAAATTCATCAATTATGT 6444 0.10397482660374394 No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA 6423 0.10363598871443937 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 6404 0.10332942110030667 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 6381 0.10295831293583024 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 6374 0.10284536697272872 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 6329 0.10211928578136178 No Hit
ACTGAGTCACACTGCATAGGAATTTAGAACCTAACTTTTATAGGTTATCA 6314 0.1018772587175728 No Hit
TAACATGCCCCACAAAGTTTCTATGTATATATTAGGACAAAATTGTGCAA 6300 0.10165136679136975 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 6293 0.10153842082826824 No Hit
AGGACAACAGCCGATCCATAAATTTGCTGACAGGTGTATCTGCGTTTCCC 6276 0.10126412348930741 No Hit
CCCCAGCAGCAGACCTCCTCGTCTCCACCTCCACCTCTGCTGACTCCACC 6275 0.10124798835172148 No Hit
CTCCTCCTCCAGCTCACTGCTGCTGCTGCCGCTGCCACCGCTTCTGCTGC 6262 0.10103823156310436 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6260 0.1010059612879325 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6247 0.10079620449931538 No Hit
AGTTCTAACATTTTGTTTATGGCTTTTTGGGGGGTTTTCATTGCAGGATG 6236 0.10061871798587015 No Hit
TAAGCAATGCCACAGGGGCATGATATCCTGGCTTCTTCTGACCAAGCAAA 6223 0.10040896119725302 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6223 0.10040896119725302 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 6217 0.10031215037173744 No Hit
AGCCCACCCACAGACCCACCTTCCAGCAGATCTGCTCCTTCCTTCAGGAG 6210 0.1001992044086359 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3124280 3.3452837 4.639407 25-29
GGGGG 960970 3.281688 19.8959 4
AAAAA 3312230 3.274183 8.652801 2
CCTCC 1077215 2.659942 7.191438 6
CCAGC 1065730 2.650306 6.21817 15-19
CCTGG 1006310 2.6021957 5.8790674 35-39
CTGGG 972405 2.573206 5.8222384 5
GGAGG 962295 2.564571 5.040294 9
CCCCA 1037330 2.5208476 7.0176578 1
CAGCC 972795 2.419191 5.4608645 8
TGGGG 872280 2.3621228 19.029146 3
GGGAG 856010 2.2813153 13.259697 6
CTCCC 901210 2.2253368 10.6572895 3
GCTGG 819670 2.1690342 5.988822 3
GCCTG 825580 2.13485 5.550621 35-39
CAGGC 807840 2.0558593 6.6011124 1
TCCAG 1014530 2.0473497 6.246241 4
AAGAA 1589875 2.0138469 7.4866595 2
GGTGG 740690 2.0057788 7.272539 7
GGGGA 752425 2.0052555 13.589286 5
GAGGG 750825 2.0009913 5.5731654 8
CCCAC 822465 1.9986974 8.060098 9
GCCCA 790050 1.9647323 11.933424 2
CTGGC 759770 1.964673 5.297895 40-44
TGCAG 944690 1.9509029 5.5104995 3
CTGTG 921800 1.9342971 5.070101 5
AGAAG 1181735 1.9180704 5.6067634 3
CTCCT 944425 1.8924112 5.106958 4
GGCCC 593380 1.8908747 13.216124 1
GCCAG 733945 1.8678052 6.158669 4
GCAGA 888680 1.806141 5.389471 8
AGCTG 874480 1.8059105 9.991074 9
AGCCC 722300 1.7962484 7.8469257 1
GGCTC 691605 1.7884068 17.536486 1
TGGCT 851920 1.7876614 5.180528 3
CAGCT 878375 1.7725852 9.766677 8
AAACA 1430005 1.7700348 5.9419684 2
TCCTC 882915 1.7691593 5.1293726 5
AAAGA 1339935 1.6972554 5.6745944 2
AGGGG 613835 1.6359051 5.0426135 6
CTTGG 775935 1.6282153 10.281048 1
TTGGG 737450 1.5835736 10.313452 2
AAAAC 1274735 1.5778443 6.06455 2
GCTCC 619770 1.5661001 18.852644 2
GTGGC 591150 1.564318 5.800914 8
TGGCC 602135 1.5570481 5.1952972 9
CATGG 753345 1.5557516 32.439648 1
ACAGC 772855 1.5349176 10.60301 7
TTCAG 933055 1.5279626 6.466174 95-96
TGCCC 603445 1.5248482 9.159532 3
TAAAA 1517925 1.5246596 13.623445 1
ATGGA 918380 1.5146315 7.8126836 2
GGGTG 551125 1.4924393 7.62855 6
CTCAG 725745 1.4645735 5.0655093 3
AACAG 922945 1.463865 7.984454 6
CAACA 891285 1.3814102 8.90588 5
GCAGT 652485 1.3474633 5.6133776 4
GCACA 675250 1.3410707 7.832796 2
AGGAC 654750 1.3307049 8.873725 1
TGCTC 640795 1.3139727 13.609473 1
ATGTG 783595 1.313156 6.0988216 2
AACTG 814600 1.3128338 5.015039 2
AGCAC 656305 1.3034451 7.5767097 1
GCTCT 624480 1.2805184 9.998537 2
ATGAA 990075 1.2743 6.7186475 2
ATGGC 601665 1.2425135 8.154412 2
GGACA 608485 1.2366766 8.926572 2
ATGGG 577060 1.2195133 11.632818 2
AGTGT 722805 1.2112836 8.388467 9
GGCAC 459670 1.169807 9.85328 1
GGAGT 551975 1.1665007 11.257757 7
ATGCC 577015 1.1644324 9.531255 2
GAGTG 526300 1.1122411 10.388612 8
CATGA 672280 1.0834665 22.214586 1
CATGT 652990 1.0693306 23.755638 1
GGACC 416510 1.0599697 7.4588294 95-96
ATGTT 794595 1.0559167 6.2856994 2
AACAC 673685 1.0441501 5.1088614 2
TAAAT 1020420 1.0414577 5.768593 1
GAGAC 509640 1.0357853 6.456931 2
ATGGT 612550 1.0265172 6.2278137 2
GACAA 644855 1.0227919 7.918593 3
TAAAG 776355 0.99922645 9.999214 1
ATGAC 616050 0.99284464 5.6526976 2
ATGCT 601655 0.9852649 5.7737303 2
GCACT 485695 0.98014605 5.5550838 2
ACAAC 630375 0.9770236 9.181256 4
ATGTA 719065 0.94039834 6.551272 2
AGACT 577430 0.93060356 5.046706 1
CGCCC 297925 0.9277214 6.6685386 9
CATGC 459235 0.92674893 24.677526 1
GCGCC 283085 0.90208346 6.46636 8
AGACC 452760 0.8991975 5.419755 3
TAATT 804230 0.8340327 7.387878 1
TAAGA 627200 0.8072529 7.6293254 1
GCCCG 250110 0.7970047 5.1778836 2
ATGCA 471270 0.7595129 7.1942744 2
TAATG 576110 0.7534407 8.04571 1
TAACT 577010 0.7374079 8.928995 1
TAAGT 549990 0.7192808 9.867414 1
TAAAC 545105 0.6855901 7.811978 1
TAATA 663365 0.6770415 5.438453 1
CCGCG 209220 0.66670394 5.6299977 6
TAAGG 399640 0.65910333 8.001471 1
TAACA 522215 0.6568008 7.0898275 1
TCCCG 233775 0.59072727 5.9668474 4
TAATC 446990 0.5712448 6.1966577 1
CTCCG 225715 0.57036036 5.104184 3
TGCCG 201500 0.52105457 6.002704 5
Produced by FastQC (version 0.10.0)