##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 8016-8-31.h.sapiens.aligned.bam File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5852444 Filtered Sequences 0 Sequence length 1-100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.986017978130164 24.0 24.0 31.0 23.0 31.0 2 31.03222664635496 31.0 31.0 32.0 29.0 33.0 3 32.68962995565927 33.0 33.0 34.0 29.0 35.0 4 33.9025713366335 34.0 33.0 36.0 29.0 37.0 5 35.44407311123158 36.0 35.0 37.0 33.0 37.0 6 35.7577865905028 36.0 36.0 38.0 33.0 38.0 7 35.7836541995685 37.0 35.0 37.0 34.0 37.0 8 35.62234520269072 36.0 36.0 37.0 32.0 38.0 9 35.65035416207917 37.0 36.0 37.0 31.0 38.0 10-14 36.0108063694746 37.0 36.0 37.4 32.4 38.0 15-19 36.99882134931823 38.0 37.2 38.4 32.8 39.4 20-24 36.612451413707895 37.4 36.0 38.8 32.8 39.8 25-29 36.26909244338756 37.6 36.2 38.0 32.8 38.2 30-34 36.1102138413796 37.6 35.6 38.2 32.4 38.6 35-39 36.137239244394564 37.8 36.4 38.0 31.6 38.6 40-44 36.06190223399204 37.4 35.8 38.4 32.4 38.8 45-49 36.0285334074724 37.6 35.8 38.2 32.0 38.6 50-54 36.23587402209032 37.6 36.2 38.2 33.0 39.0 55-59 35.946905009510445 37.6 35.8 38.2 32.4 38.6 60-64 35.94109465103773 37.6 35.6 38.2 32.6 38.6 65-69 35.74745301266036 37.4 36.0 38.2 31.4 38.6 70-74 35.79515388191838 37.4 36.0 38.4 31.6 39.0 75-79 35.4992874739641 37.4 35.4 37.6 31.6 38.4 80-84 35.606289055352306 37.0 35.8 38.2 31.0 38.6 85-89 35.3154265024764 37.2 35.4 37.6 31.0 38.2 90-94 35.32635739544927 37.0 35.0 38.2 30.8 38.4 95-99 35.145297456291175 37.4 35.2 37.6 30.6 38.2 100 34.03078884581915 36.0 33.0 38.0 28.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 11.0 2 34.0 3 149.0 4 0.0 5 12.0 6 33.0 7 96.0 8 177.0 9 5730.0 10 503.0 11 686.0 12 1156.0 13 2060.0 14 2557.0 15 3218.0 16 3840.0 17 5166.0 18 6728.0 19 8403.0 20 9268.0 21 9885.0 22 10871.0 23 12930.0 24 16277.0 25 21748.0 26 29385.0 27 39531.0 28 53851.0 29 71303.0 30 92882.0 31 120500.0 32 157227.0 33 211418.0 34 312493.0 35 560036.0 36 1674007.0 37 2408264.0 38 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.226710386785125 19.221559663091334 20.98849745860555 40.563232491517994 2 17.897502314066013 51.40165428991317 15.399405826878954 15.301437569141862 3 18.46913793127043 34.54677858383318 30.324704161243925 16.659379323652455 4 34.44278209902047 24.54265113585525 20.82204834602333 20.192518419100946 5 20.85193789800306 27.23903354605798 26.034303132275117 25.87472542366384 6 19.949873261921198 29.21768624196211 25.999140068051407 24.83330042806529 7 22.43635470730388 27.785282096211738 26.983965966511757 22.794397229972624 8 21.701849542909354 27.280449461615287 28.834461694110843 22.183239301364523 9 21.62036836321344 28.851057106562667 26.674588161386687 22.8539863688372 10-14 22.12714391036968 27.405623353818985 26.563648539478063 23.90358419633327 15-19 21.521816172830675 27.168555887772555 27.127980623434222 24.181647315962547 20-24 21.80673373661419 26.584228928054053 27.52337987750777 24.085657457823988 25-29 22.038585538544687 26.901049729247497 27.997954948224873 23.062409783982947 30-34 22.46780383624393 27.38178638937349 27.365177462941055 22.785232311441522 35-39 22.447295213806665 26.296124272541775 28.476612948004426 22.779967565647137 40-44 22.552156595098218 27.351446053694833 27.384041600776314 22.71235575043064 45-49 22.045291544156438 27.86172041114252 27.90371501197525 22.189273032725794 50-54 22.70001691010784 27.342418150979057 27.485007737381718 22.472557201531387 55-59 22.051591485600976 28.138691796318927 27.31791116990483 22.491805548175268 60-64 22.549004735671577 27.602292770888226 28.002072098851706 21.846630394588498 65-69 22.564759928175345 28.52999823426103 27.522730526285084 21.38251131127854 70-74 22.31200585873021 28.183411001848206 27.306484379240658 22.198098760180923 75-79 22.826953929053893 28.03017590346353 27.409338671823054 21.73353149565953 80-84 21.927935824805857 28.150967688612788 27.571489698766065 22.349606787815286 85-89 22.26734060735078 27.838468244267826 27.855160034348575 22.039031114032817 90-94 22.31058631439102 27.507568876118878 27.776903617020537 22.40494119246957 95-99 22.472241946569298 26.77125432811942 28.08461292968244 22.671890795628844 100 23.812371914983547 26.7428863981712 26.574394605286066 22.87034708155918 >>END_MODULE >>Per base GC content fail #Base %GC 1 59.789942878303115 2 33.19893988320788 3 35.12851725492289 4 54.63530051812142 5 46.726663321666905 6 44.783173689986484 7 45.2307519372765 8 43.885088844273874 9 44.47435473205064 10-14 46.030728106702945 15-19 45.703463488793226 20-24 45.89239119443818 25-29 45.10099532252764 30-34 45.25303614768546 35-39 45.227262779453795 40-44 45.26451234552886 45-49 44.23456457688223 50-54 45.17257411163922 55-59 44.543397033776245 60-64 44.395635130260075 65-69 43.94727123945389 70-74 44.510104618911136 75-79 44.56048542471342 80-84 44.27754261262115 85-89 44.3063717213836 90-94 44.71552750686059 95-99 45.14413274219814 100 46.682718996542725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6085.0 1 6084.0 2 6084.0 3 6087.0 4 6090.5 5 6095.0 6 6109.0 7 6133.5 8 6151.5 9 6173.0 10 6190.5 11 6221.5 12 6269.5 13 6294.5 14 6339.0 15 7030.0 16 7463.0 17 7032.5 18 7252.0 19 8421.0 20 9684.5 21 11142.5 22 15434.0 23 22081.5 24 35582.0 25 48459.0 26 61394.0 27 77154.0 28 82258.5 29 97743.5 30 127385.0 31 141914.5 32 147155.5 33 164601.5 34 183841.5 35 183790.0 36 174466.5 37 165985.5 38 188540.5 39 209278.0 40 212332.0 41 225323.0 42 216790.5 43 221233.0 44 228398.5 45 208572.0 46 186750.0 47 167727.0 48 164291.5 49 172861.5 50 182443.0 51 192209.5 52 158859.5 53 105079.0 54 96665.5 55 98200.0 56 77639.5 57 66073.0 58 90840.5 59 90389.0 60 91611.0 61 113090.5 62 98841.0 63 92857.0 64 86351.0 65 73206.0 66 72785.5 67 65629.5 68 49909.5 69 43673.0 70 41769.0 71 38341.0 72 43281.5 73 30769.5 74 15447.5 75 18641.0 76 19387.0 77 19349.5 78 16935.5 79 15110.0 80 13205.0 81 10678.5 82 9258.0 83 9021.0 84 8844.5 85 7102.0 86 9524.5 87 9188.5 88 6603.0 89 6743.5 90 7221.0 91 7829.5 92 6478.5 93 5109.5 94 5054.5 95 5034.5 96 5027.0 97 5024.5 98 5021.5 99 5018.5 100 5011.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05421666572119272 2 0.007645968727132653 3 0.001967201785466553 4 0.006911229533394496 5 0.004449153702517263 6 7.362963267374881E-4 7 3.769756307530551E-4 8 1.884976990428601E-4 9 1.0281710293962666E-4 10-14 0.0012989514480052698 15-19 0.018345498594968583 20-24 0.016818997467271026 25-29 0.008442066517061968 30-34 0.007904767998514126 35-39 0.005455444575172293 40-44 0.013257916822140996 45-49 0.011872352401342587 50-54 0.010701410602913165 55-59 0.01122765532300622 60-64 0.05413678518746475 65-69 0.04371372007154698 70-74 0.027606003334284254 75-79 0.07482377442667507 80-84 0.06445945787675152 85-89 0.015110161844610311 90-94 0.0072203854872285615 95-99 0.009482219039112848 100 0.008222065717743553 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-1 6226.0 2-3 659.0 4-5 5519.0 6-7 4425.0 8-9 61.0 10-11 65.0 12-13 110.0 14-15 285.0 16-17 186.0 18-19 2603.0 20-21 31848.0 22-23 2191.0 24-25 5399.0 26-27 1004.0 28-29 1100.0 30-31 1789.0 32-33 1906.0 34-35 1454.0 36-37 1125.0 38-39 1183.0 40-41 3287.0 42-43 1544.0 44-45 2226.0 46-47 3702.0 48-49 1370.0 50-51 16971.0 52-53 2090.0 54-55 19771.0 56-57 15680.0 58-59 40202.0 60-61 2115.0 62-63 12696.0 64-65 5820.0 66-67 7045.0 68-69 6646.0 70-71 22335.0 72-73 21332.0 74-75 14789.0 76-77 16839.0 78-79 5799.0 80-81 12215.0 82-83 14053.0 84-85 10384.0 86-87 2987.0 88-89 6265.0 90-91 12627.0 92-93 1628.0 94-95 3990.0 96-97 121120.0 98-99 0.0 100-101 5375778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 97.48884054434768 #Duplication Level Relative count 1 100.0 2 7.738629868539234 3 2.397866144089709 4 1.2662965079883508 5 1.0070493454179255 6 0.9349228382461011 7 0.8825290547344928 8 0.8600745760866607 9 0.8920551971911489 10++ 12.115211888625787 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 13896 0.23743926469010213 No Hit AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 13782 0.23549136053245445 No Hit CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 11946 0.20411985146718192 No Hit GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 11847 0.20242825048817212 No Hit TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 11260 0.19239825276414435 No Hit AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 11223 0.19176603825683766 No Hit AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11219 0.19169769074253423 No Hit GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 11081 0.18933970149906604 No Hit CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 10623 0.18151391111132376 No Hit CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 10584 0.18084752284686534 No Hit CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 10181 0.17396151078079516 No Hit GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 10104 0.1726458211304542 No Hit CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 9483 0.16203486953484733 No Hit AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 9260 0.15822449561243132 No Hit GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 9198 0.15716510914072823 No Hit CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 9092 0.15535390001168742 No Hit TGAGACCGCACCAGCGACACA 9073 0.15502924931874615 No Hit TGTGTCGCTGGTGCGGTCTCA 8954 0.15299591076821922 No Hit CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 8760 0.14968105632450307 No Hit CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8737 0.14928805811725837 No Hit AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 8728 0.14913427621007566 No Hit CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 8677 0.14826284540270698 No Hit GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 8661 0.14798945534549326 No Hit TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 8658 0.14793819470976569 No Hit CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 8337 0.14245330668691575 No Hit TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 8235 0.1407104450721784 No Hit GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 8174 0.13966814547905115 No Hit CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 8173 0.13965105860047528 No Hit CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 8035 0.13729306935700708 No Hit ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7991 0.1365412466996694 No Hit GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 7765 0.13267961214152585 No Hit GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 7630 0.1303728835337852 No Hit CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 7578 0.12948436584784068 No Hit CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 7508 0.1282882843475307 No Hit CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 7449 0.12728015851155516 No Hit CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 7408 0.12657959648994507 No Hit GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 7384 0.1261695114041245 No Hit TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 7332 0.12528099371817994 No Hit GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 7291 0.12458043169656983 No Hit ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7056 0.12056501523124356 No Hit CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6976 0.11919806494517504 No Hit CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6975 0.11918097806659918 No Hit TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6951 0.11877089298077863 No Hit CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6832 0.11673755443025172 No Hit GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6796 0.11612242680152088 No Hit CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC 6781 0.11586612362288302 No Hit AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 6763 0.1155585598085176 No Hit CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 6761 0.11552438605136589 No Hit GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG 6685 0.1142257832796008 No Hit CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6379 0.10899719843538869 No Hit ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6323 0.10804033323514074 No Hit AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6315 0.10790363820653388 No Hit CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 6298 0.10761316127074433 No Hit CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 6293 0.10752772687786505 No Hit GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 6266 0.10706638115631692 No Hit TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 6228 0.10641707977043437 No Hit TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 6199 0.10592156029173452 No Hit AGGGTCAGCCCACACCACCCAGCCCCCACGCCCAACCCTCGTGCCTCCCT 6155 0.10516973763439684 No Hit CATGGGGGCCAGTTGTGGGTGGTGGCCCAGGTGCAGGAGAGGCGGGCAGT 6128 0.10470839191284871 No Hit CGAGACCGCGCCCCAGGCCCAGCACTCACAAATTCCTGGTCGTGGTTATT 6117 0.10452043624851429 No Hit ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6111 0.10441791497705917 No Hit AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 6057 0.10349522353396291 No Hit CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 5970 0.1020086650978634 No Hit TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5961 0.10185488319068069 No Hit CATGTTGTCAGATTACCCTTTAGAATGCCTGTTGCTTTTAAAATATTTTT 5901 0.10082967047612929 No Hit CAGCATAATAAAAAATAGGATTCCCAGCTTTGGAAGCCCCAGCTTGGTAG 5890 0.10064171481179486 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 2935965 3.535779 4.3074064 60-64 AAAAA 3140435 3.5087388 6.8881164 2 GGGGG 879950 2.9536047 9.953803 4 GGAGG 1025820 2.763242 5.5398145 4 CCAGC 1048670 2.6541183 8.545669 5 CCAGG 1020930 2.6381502 5.12057 5 CCTCC 1037545 2.6108437 9.407084 6 CCCCA 1039925 2.577872 6.037766 1 CAGCC 1017125 2.57428 6.757239 6 CCTGG 976715 2.5620406 5.599723 9 TGGGG 872100 2.3846717 11.621633 3 GGGAG 884170 2.3816807 5.1321106 6 CTCCC 919815 2.314592 5.3480635 3 AGCAG 1084400 2.2959821 5.0650535 5 GCTGG 812410 2.1757824 6.56975 7 GAGGG 787055 2.1200829 5.9786744 9 GGGGA 765040 2.0607812 5.6985335 5 CTCCT 965155 2.020045 6.1811886 4 AAGAA 1436220 1.999534 5.181165 2 CTCCA 951550 1.9619188 5.8001595 6 TCCAG 911055 1.9178565 6.039202 7 CCCAC 767745 1.9031644 5.900084 9 GCCAG 727905 1.8809543 7.390736 4 TGGCT 860350 1.8770826 7.2235627 3 TGTTT 1267765 1.8741529 5.7798314 3 TGCAG 857925 1.8439238 5.22606 3 CACCA 904510 1.8371652 5.1993265 8 GCCCA 723480 1.8310827 5.805959 4 TCCTC 873285 1.8277636 6.884612 5 GGCTC 694115 1.8207469 8.318259 1 AGGGG 626470 1.6875165 7.3128476 6 GGCCC 521775 1.6455507 5.7559466 1 TAAAA 1386945 1.5730231 8.615776 1 GCTCC 607440 1.5606279 9.157325 2 TGCCC 605275 1.5550659 12.260775 3 GATGG 690135 1.514432 6.1846266 1 ATGGA 843620 1.4856482 10.758076 2 CATGG 663915 1.4269414 42.4928 1 GGGGT 488900 1.336849 5.066564 4 TGCTC 601995 1.2864089 6.307532 1 ATGTG 718780 1.2849306 8.083219 2 TGGGT 575185 1.2812618 6.25082 3 ATGGG 568420 1.2473407 15.396496 2 ATGAA 880350 1.2441645 8.935082 2 GGCAC 472990 1.2222371 5.250566 1 ATGGC 563725 1.2116046 11.605141 2 TGAGA 679560 1.1967319 5.892633 1 ATGCC 566275 1.1920621 12.737736 2 GAGAC 519995 1.1009768 6.2457395 2 CATGT 616000 1.0785551 32.937214 1 ATGTT 738625 1.0756617 8.367646 2 CATGA 607155 1.0472409 28.761377 1 TAAAG 733235 1.0362527 6.561124 1 ATGCT 562670 0.9851794 8.410255 2 CACTC 473340 0.975939 5.3884416 1 ATGGT 544050 0.9725737 7.6390305 2 ATGAT 673660 0.96644646 6.574178 2 AGACC 464225 0.9626877 5.4764414 3 ATGAC 557850 0.96219784 6.7603984 2 ATGAG 540305 0.95149845 5.1478896 2 ATGTA 655965 0.94106084 9.468884 2 GCGCC 291525 0.91939855 5.1861577 8 CATGC 435165 0.91606337 33.08712 1 TAAGA 574755 0.8122789 5.333268 1 TAATG 539080 0.7733752 5.1427097 1 TAACT 534075 0.7504422 5.4905806 1 TAAGT 521235 0.7477744 6.540141 1 ATGCA 426860 0.736262 8.958174 2 TAAAC 499450 0.6913411 5.400415 1 TGCCG 222290 0.58309335 5.9373674 5 >>END_MODULE