FastQCFastQC Report
Wed 20 Mar 2013
8016-8-31.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-31.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5852444
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 13896 0.23743926469010213 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 13782 0.23549136053245445 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 11946 0.20411985146718192 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 11847 0.20242825048817212 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 11260 0.19239825276414435 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 11223 0.19176603825683766 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11219 0.19169769074253423 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 11081 0.18933970149906604 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 10623 0.18151391111132376 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 10584 0.18084752284686534 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 10181 0.17396151078079516 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 10104 0.1726458211304542 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 9483 0.16203486953484733 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 9260 0.15822449561243132 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 9198 0.15716510914072823 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 9092 0.15535390001168742 No Hit
TGAGACCGCACCAGCGACACA 9073 0.15502924931874615 No Hit
TGTGTCGCTGGTGCGGTCTCA 8954 0.15299591076821922 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 8760 0.14968105632450307 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8737 0.14928805811725837 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 8728 0.14913427621007566 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 8677 0.14826284540270698 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 8661 0.14798945534549326 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 8658 0.14793819470976569 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 8337 0.14245330668691575 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 8235 0.1407104450721784 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 8174 0.13966814547905115 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 8173 0.13965105860047528 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 8035 0.13729306935700708 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7991 0.1365412466996694 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 7765 0.13267961214152585 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 7630 0.1303728835337852 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 7578 0.12948436584784068 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 7508 0.1282882843475307 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 7449 0.12728015851155516 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 7408 0.12657959648994507 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 7384 0.1261695114041245 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 7332 0.12528099371817994 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 7291 0.12458043169656983 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7056 0.12056501523124356 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6976 0.11919806494517504 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6975 0.11918097806659918 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6951 0.11877089298077863 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6832 0.11673755443025172 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6796 0.11612242680152088 No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC 6781 0.11586612362288302 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 6763 0.1155585598085176 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 6761 0.11552438605136589 No Hit
GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG 6685 0.1142257832796008 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6379 0.10899719843538869 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6323 0.10804033323514074 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6315 0.10790363820653388 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 6298 0.10761316127074433 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 6293 0.10752772687786505 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 6266 0.10706638115631692 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 6228 0.10641707977043437 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 6199 0.10592156029173452 No Hit
AGGGTCAGCCCACACCACCCAGCCCCCACGCCCAACCCTCGTGCCTCCCT 6155 0.10516973763439684 No Hit
CATGGGGGCCAGTTGTGGGTGGTGGCCCAGGTGCAGGAGAGGCGGGCAGT 6128 0.10470839191284871 No Hit
CGAGACCGCGCCCCAGGCCCAGCACTCACAAATTCCTGGTCGTGGTTATT 6117 0.10452043624851429 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6111 0.10441791497705917 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 6057 0.10349522353396291 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 5970 0.1020086650978634 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5961 0.10185488319068069 No Hit
CATGTTGTCAGATTACCCTTTAGAATGCCTGTTGCTTTTAAAATATTTTT 5901 0.10082967047612929 No Hit
CAGCATAATAAAAAATAGGATTCCCAGCTTTGGAAGCCCCAGCTTGGTAG 5890 0.10064171481179486 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2935965 3.535779 4.3074064 60-64
AAAAA 3140435 3.5087388 6.8881164 2
GGGGG 879950 2.9536047 9.953803 4
GGAGG 1025820 2.763242 5.5398145 4
CCAGC 1048670 2.6541183 8.545669 5
CCAGG 1020930 2.6381502 5.12057 5
CCTCC 1037545 2.6108437 9.407084 6
CCCCA 1039925 2.577872 6.037766 1
CAGCC 1017125 2.57428 6.757239 6
CCTGG 976715 2.5620406 5.599723 9
TGGGG 872100 2.3846717 11.621633 3
GGGAG 884170 2.3816807 5.1321106 6
CTCCC 919815 2.314592 5.3480635 3
AGCAG 1084400 2.2959821 5.0650535 5
GCTGG 812410 2.1757824 6.56975 7
GAGGG 787055 2.1200829 5.9786744 9
GGGGA 765040 2.0607812 5.6985335 5
CTCCT 965155 2.020045 6.1811886 4
AAGAA 1436220 1.999534 5.181165 2
CTCCA 951550 1.9619188 5.8001595 6
TCCAG 911055 1.9178565 6.039202 7
CCCAC 767745 1.9031644 5.900084 9
GCCAG 727905 1.8809543 7.390736 4
TGGCT 860350 1.8770826 7.2235627 3
TGTTT 1267765 1.8741529 5.7798314 3
TGCAG 857925 1.8439238 5.22606 3
CACCA 904510 1.8371652 5.1993265 8
GCCCA 723480 1.8310827 5.805959 4
TCCTC 873285 1.8277636 6.884612 5
GGCTC 694115 1.8207469 8.318259 1
AGGGG 626470 1.6875165 7.3128476 6
GGCCC 521775 1.6455507 5.7559466 1
TAAAA 1386945 1.5730231 8.615776 1
GCTCC 607440 1.5606279 9.157325 2
TGCCC 605275 1.5550659 12.260775 3
GATGG 690135 1.514432 6.1846266 1
ATGGA 843620 1.4856482 10.758076 2
CATGG 663915 1.4269414 42.4928 1
GGGGT 488900 1.336849 5.066564 4
TGCTC 601995 1.2864089 6.307532 1
ATGTG 718780 1.2849306 8.083219 2
TGGGT 575185 1.2812618 6.25082 3
ATGGG 568420 1.2473407 15.396496 2
ATGAA 880350 1.2441645 8.935082 2
GGCAC 472990 1.2222371 5.250566 1
ATGGC 563725 1.2116046 11.605141 2
TGAGA 679560 1.1967319 5.892633 1
ATGCC 566275 1.1920621 12.737736 2
GAGAC 519995 1.1009768 6.2457395 2
CATGT 616000 1.0785551 32.937214 1
ATGTT 738625 1.0756617 8.367646 2
CATGA 607155 1.0472409 28.761377 1
TAAAG 733235 1.0362527 6.561124 1
ATGCT 562670 0.9851794 8.410255 2
CACTC 473340 0.975939 5.3884416 1
ATGGT 544050 0.9725737 7.6390305 2
ATGAT 673660 0.96644646 6.574178 2
AGACC 464225 0.9626877 5.4764414 3
ATGAC 557850 0.96219784 6.7603984 2
ATGAG 540305 0.95149845 5.1478896 2
ATGTA 655965 0.94106084 9.468884 2
GCGCC 291525 0.91939855 5.1861577 8
CATGC 435165 0.91606337 33.08712 1
TAAGA 574755 0.8122789 5.333268 1
TAATG 539080 0.7733752 5.1427097 1
TAACT 534075 0.7504422 5.4905806 1
TAAGT 521235 0.7477744 6.540141 1
ATGCA 426860 0.736262 8.958174 2
TAAAC 499450 0.6913411 5.400415 1
TGCCG 222290 0.58309335 5.9373674 5
Produced by FastQC (version 0.10.0)