##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-32.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5737366	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.9625952048379	24.0	24.0	31.0	23.0	31.0
2	31.018382064242644	31.0	31.0	32.0	29.0	33.0
3	32.6678225303905	33.0	33.0	34.0	29.0	35.0
4	33.881109028050815	34.0	33.0	36.0	29.0	37.0
5	35.41648120509673	36.0	35.0	37.0	33.0	37.0
6	35.72992587445794	36.0	36.0	38.0	33.0	38.0
7	35.75914405864773	37.0	35.0	37.0	34.0	37.0
8	35.596563238003185	36.0	36.0	37.0	32.0	38.0
9	35.62600429217608	37.0	36.0	37.0	31.0	38.0
10-14	35.98979083980464	37.0	36.0	37.4	32.4	38.0
15-19	36.97447798040052	38.0	37.2	38.4	32.6	39.4
20-24	36.59525115173616	37.4	36.0	38.8	32.8	39.8
25-29	36.25219041828009	37.6	36.2	38.0	32.8	38.2
30-34	36.08661602509763	37.6	35.6	38.2	32.4	38.6
35-39	36.11858249082031	37.8	36.4	38.0	31.6	38.6
40-44	36.043691940851694	37.4	35.8	38.4	31.8	38.8
45-49	36.00447794672018	37.6	35.8	38.2	31.8	38.6
50-54	36.21665599564088	37.6	36.2	38.2	33.0	39.0
55-59	35.92847188294455	37.6	35.8	38.2	32.4	38.6
60-64	35.92666529850048	37.6	35.6	38.2	32.6	38.6
65-69	35.732206605256394	37.4	36.0	38.2	31.4	38.6
70-74	35.777020363153554	37.4	36.0	38.4	31.6	39.0
75-79	35.48598428605581	37.4	35.4	37.6	31.6	38.4
80-84	35.5983550523016	37.0	35.8	38.2	31.0	38.6
85-89	35.31297302005359	37.2	35.4	37.6	31.0	38.2
90-94	35.33452559837813	37.0	35.0	38.2	30.8	38.4
95-99	35.15210048942824	37.4	35.2	37.6	30.8	38.2
100	34.071624248682696	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	7.0
2	27.0
3	126.0
4	0.0
5	10.0
6	39.0
7	143.0
8	252.0
9	6421.0
10	532.0
11	706.0
12	1143.0
13	2211.0
14	2723.0
15	3452.0
16	4172.0
17	5307.0
18	6934.0
19	8807.0
20	9982.0
21	10688.0
22	11326.0
23	13229.0
24	16068.0
25	21223.0
26	28511.0
27	39045.0
28	52400.0
29	69529.0
30	90336.0
31	117249.0
32	152547.0
33	206272.0
34	304121.0
35	544180.0
36	1620798.0
37	2386842.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.481245799181224	20.101954417297335	21.595097808154605	39.821701975366835
2	17.05138094357206	53.11840548398469	15.03125578083607	14.798957791607187
3	18.200723863771213	35.67463475244138	30.364741482210544	15.759899901576865
4	33.64735288481575	25.229384537037134	21.060689038443616	20.0625735397035
5	20.23317404742202	27.804558159197825	26.63664572042678	25.32562207295338
6	19.647762499984726	29.916462220152102	26.544923444505798	23.890851835357374
7	21.777636885393363	28.579350649123818	27.574828702223947	22.068183763258876
8	20.88267843007662	27.929545995999526	29.40867626006843	21.779099313855426
9	21.09291448111775	29.325675157114123	27.25795709812085	22.323453263647284
10-14	21.70493005902866	27.878997796577753	27.106313595106535	23.309758549287054
15-19	21.179829293763767	27.583525061204877	27.642659548676978	23.593986096354378
20-24	21.455142467843963	27.01418341627283	27.972256857035728	23.558417258847477
25-29	21.627206782097133	27.17668532191235	28.549802380554436	22.646305515436087
30-34	22.144940494847365	27.66393049974526	27.79940927821144	22.391719727195934
35-39	22.170616919525497	26.475856649314927	28.993143117963328	22.36038331319625
40-44	22.220638960127857	27.762512564159092	27.617574819699474	22.399273656013573
45-49	21.813040069157335	28.345698124622302	28.079787282606794	21.76147452361357
50-54	22.294342250501114	27.689395733032185	27.911232482614917	22.105029533851784
55-59	21.713656558088744	28.467035522073793	27.62941637427042	22.189891545567043
60-64	22.185379989400634	27.91011756763986	28.39976357748466	21.50473886547485
65-69	22.187138296280615	28.86869138628734	27.917840948818174	21.02632936861387
70-74	21.856383695323444	28.492593334851545	27.720953387327263	21.930069582497747
75-79	22.477874885180142	28.2900237287031	27.7840090617049	21.448092324411856
80-84	21.725032370378162	28.411340775214416	27.88658398158659	21.977042872820828
85-89	21.941277415198837	28.078201504960727	28.148571097168627	21.83194998267181
90-94	21.873286235399807	27.942888101939587	28.157094728798565	22.02673093386204
95-99	22.021747582112997	27.180886496331464	28.428478095371172	22.36888782618437
100	23.385351248206856	27.278390582659252	26.87234842208172	22.46390974705217
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	58.30294777454805
2	31.850338735179246
3	33.960623765348075
4	53.70992642451925
5	45.5587961203754
6	43.538614335342096
7	43.845820648652236
8	42.66177774393204
9	43.41636774476503
10-14	45.01468860831571
15-19	44.773815390118145
20-24	45.01355972669144
25-29	44.273512297533216
30-34	44.5366602220433
35-39	44.531000232721745
40-44	44.619912616141434
45-49	43.5745145927709
50-54	44.3993717843529
55-59	43.90354810365578
60-64	43.69011885487548
65-69	43.21346766489448
70-74	43.78645327782119
75-79	43.925967209591995
80-84	43.70207524319899
85-89	43.77322739787065
90-94	43.900017169261844
95-99	44.39063540829737
100	45.84926099525903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6679.0
1	6680.5
2	6682.0
3	6681.0
4	6684.0
5	6689.5
6	6699.5
7	6710.0
8	6723.5
9	6748.0
10	6792.0
11	6839.5
12	6875.5
13	6926.0
14	7016.0
15	7895.5
16	8426.5
17	7998.5
18	8292.0
19	9437.0
20	10600.0
21	12197.5
22	16598.0
23	24167.5
24	39395.5
25	53866.5
26	66444.0
27	83424.0
28	89141.0
29	104713.5
30	135509.5
31	151808.5
32	157506.5
33	172625.5
34	190092.0
35	187680.0
36	179288.0
37	170839.5
38	195525.0
39	214699.0
40	218390.0
41	232620.5
42	219539.0
43	225517.0
44	229994.5
45	208707.0
46	191508.5
47	169432.0
48	156892.5
49	168352.5
50	182838.5
51	189830.5
52	154762.5
53	99480.0
54	88120.5
55	87806.5
56	69848.0
57	60873.5
58	82747.5
59	78718.5
60	74722.5
61	93434.5
62	82470.5
63	72720.0
64	69571.5
65	64665.5
66	62406.5
67	55529.0
68	41715.5
69	35934.5
70	33842.0
71	29272.5
72	35139.0
73	25745.0
74	12473.0
75	14922.5
76	14941.0
77	13709.5
78	11896.0
79	12015.0
80	10089.5
81	7568.5
82	6328.5
83	5793.5
84	5576.5
85	4353.5
86	6672.0
87	6317.5
88	3744.0
89	3670.0
90	3761.0
91	3978.5
92	3062.0
93	2140.0
94	2091.0
95	2067.0
96	2059.0
97	2061.0
98	2054.0
99	2046.0
100	2044.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05655905514830325
2	0.0077827991762099025
3	0.002286017027162245
4	0.007905274912479183
5	0.005026705244302238
6	9.252599456715293E-4
7	5.065113784287982E-4
8	1.2226367348892143E-4
9	6.98653223647106E-5
10-14	0.0013029976841574134
15-19	0.01662324173757107
20-24	0.014957197247679408
25-29	0.009334539715146526
30-34	0.00866224097869261
35-39	0.0058511282280556565
40-44	0.012328436738375255
45-49	0.0116703306076714
50-54	0.010890846105944383
55-59	0.011511899179128926
60-64	0.052515939603469244
65-69	0.042699008898777986
70-74	0.02618885220835226
75-79	0.07374000118952045
80-84	0.06300534228802845
85-89	0.014678417161653865
90-94	0.007107479936432128
95-99	0.01034323428268767
100	0.009628606532809334
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	6780.0
2-3	235.0
4-5	2231.0
6-7	2789.0
8-9	30.0
10-11	30.0
12-13	58.0
14-15	180.0
16-17	167.0
18-19	3113.0
20-21	22414.0
22-23	2609.0
24-25	3199.0
26-27	1524.0
28-29	1294.0
30-31	1774.0
32-33	1511.0
34-35	2106.0
36-37	1842.0
38-39	1411.0
40-41	2905.0
42-43	1945.0
44-45	1827.0
46-47	2646.0
48-49	1457.0
50-51	19467.0
52-53	2770.0
54-55	22447.0
56-57	17540.0
58-59	42503.0
60-61	2995.0
62-63	10343.0
64-65	5947.0
66-67	8552.0
68-69	6404.0
70-71	28058.0
72-73	24535.0
74-75	16125.0
76-77	20265.0
78-79	7313.0
80-81	14101.0
82-83	16514.0
84-85	8841.0
86-87	2748.0
88-89	6615.0
90-91	14642.0
92-93	1537.0
94-95	3807.0
96-97	122432.0
98-99	0.0
100-101	5244788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.32930987723266
#Duplication Level	Relative count
1	100.0
2	7.07642908886815
3	2.1092888329080384
4	1.0474655250053841
5	0.71332075939619
6	0.5331958466849838
7	0.4875119920118517
8	0.4986066424324695
9	0.47576471509590346
10++	12.066411272164828
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	13278	0.23143024168233295	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	13225	0.2305064728309123	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	12182	0.21232739901899234	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	12062	0.2102358469025682	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	10635	0.1853638063180909	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	10591	0.18459690387540206	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	10331	0.18006520762314973	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	10215	0.17804337391060635	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	10119	0.17637013221746703	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	10105	0.1761261178038842	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	9720	0.1694157214303567	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	9495	0.16549406121206142	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	9435	0.16444828515384935	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	9370	0.16331536109078626	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	9346	0.16289705066750143	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	9264	0.16146782338794494	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	9185	0.16009088491129903	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	9078	0.1582259176074875	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	9033	0.15744158556382842	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	8981	0.15653524631337795	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	8884	0.15484457501926843	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	8780	0.1530318965183675	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	8421	0.14677466977006523	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	8357	0.14565917530797232	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	8355	0.14562431610603194	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	8352	0.14557202730312133	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	8349	0.14551973850021074	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	8287	0.14443910324005824	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	8272	0.1441776592255052	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	7440	0.12967623121829774	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	7400	0.1289790471794897	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	7385	0.12871760316493666	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	7340	0.1279332711212776	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	7258	0.1265040438417211	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	7134	0.12434277332141613	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	6932	0.12082199392543545	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	6929	0.12076970512252486	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	6851	0.11941019624684915	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	6807	0.1186432938041603	No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG	6804	0.11859100500124971	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	6799	0.1185038569963987	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	6572	0.114547337576163	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	6519	0.11362356872474233	No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC	6515	0.11355385032086152	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	6501	0.11330983590727871	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	6465	0.11268237027235146	No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG	6456	0.11252550386361963	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	6311	0.10999821172294046	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	6305	0.10989363411711925	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	6301	0.10982391571323843	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	6285	0.10954504209771522	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	6153	0.10724433476964866	No Hit
*	6071	0.10581510749009214	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	6068	0.10576281868718154	No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA	6061	0.10564081148039012	No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC	6014	0.10482162023479066	No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG	5855	0.10205031368052866	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	5854	0.10203288407955846	No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA	5853	0.10201545447858826	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	5846	0.10189344727179685	No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT	5800	0.1010916856271676	No Hit
TGAGACCGCACCAGCGACACA	5775	0.10065594560291255	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3025135	3.4604588	4.368015	60-64
AAAAA	3235020	3.4380634	6.9279413	2
GGGGG	821865	3.0656583	8.587871	4
GGAGG	967305	2.806916	5.193911	4
CCAGG	958625	2.6724758	5.585862	5
CCAGC	968465	2.646357	8.624322	5
CCTCC	969650	2.6355393	9.825973	6
CCCCA	963510	2.5805976	6.025041	1
CCTGG	910800	2.5767865	5.595827	9
CAGCC	930565	2.5427945	6.5489316	6
TGGGG	818160	2.409321	11.113515	3
CTCCC	855310	2.3247595	5.031545	3
GCTGG	770345	2.2235208	6.111386	7
GAGGG	743830	2.1584387	5.9296055	9
GGGGA	725020	2.1038558	5.409418	7
CCACC	780195	2.0896196	5.032563	1
CTCCT	922805	2.020217	5.776799	4
AAGAA	1451960	1.9835763	5.414778	2
CTCCA	908290	1.9593961	5.218607	6
TCCAG	878220	1.932865	6.040366	4
CCCAC	712755	1.9089931	6.632244	9
TGGCT	836745	1.9066991	6.6153293	3
GCCAG	683660	1.9059225	7.8129754	4
TGTTT	1294140	1.8751549	5.817465	3
CCCTG	673125	1.8665977	5.0911446	1
CACCA	876140	1.8624334	5.2299004	8
TGCAG	826805	1.8565292	5.9786286	3
GGCTC	643255	1.8198625	7.4431195	1
TCCTC	824210	1.8043716	6.6739197	5
GCCCA	655755	1.7918687	6.141497	4
AAACA	1321365	1.7693611	5.021781	2
AAAGA	1294230	1.7680953	5.1272335	2
AGGGG	596035	1.7295686	7.213935	6
CCAGA	772140	1.6745719	5.093198	8
GGCCC	459080	1.6125388	5.405267	1
GCTCC	557235	1.5452309	8.186909	2
TGCCC	556610	1.5434977	12.214443	3
TAAAA	1405395	1.5157436	8.5643	1
GATGG	659565	1.5109732	5.757933	1
ATGGA	830220	1.4795685	10.498762	2
CATGG	642885	1.4435505	42.98353	1
GGGGT	455705	1.3419619	5.1647944	4
GCAGT	590345	1.3255758	5.6120086	4
TGCTC	579560	1.294455	5.6827593	1
ATGTG	715485	1.2939957	7.959279	2
TGGGT	550850	1.2806277	6.355383	3
ATGAA	895575	1.241614	8.6679535	2
ATGGG	538500	1.2336298	15.643007	2
ATGGC	545425	1.2247113	11.036128	2
AGACA	709995	1.2220987	5.3055983	2
ATGCC	538340	1.1848267	12.147256	2
CATGT	612430	1.085646	33.15106	1
ATGTT	737840	1.0534832	8.179534	2
CATGA	598460	1.0453856	29.060974	1
TAAAG	745180	1.0331082	7.2461634	1
ATGCT	564495	1.0006723	8.004553	2
ATGGT	548140	0.99134266	7.6530557	2
ATGAC	557175	0.9732693	7.194789	2
CACTC	451015	0.9729458	5.54117	1
ATGAT	675280	0.9500773	6.5188556	2
ATGTA	669440	0.9418607	9.4314785	2
CATGC	415740	0.9149977	32.879753	1
TAAGA	587155	0.8140243	5.3209453	1
TAACT	550305	0.7588885	5.955355	1
TAAGT	536620	0.7549912	6.6321073	1
ATGCA	425505	0.7432691	9.149948	2
TAACA	507565	0.6897247	5.0450153	1
TAAAC	506455	0.6882164	5.778721	1
TAAGG	368165	0.6561216	5.406502	1
TGCCG	202440	0.57273245	6.710521	5
>>END_MODULE