FastQCFastQC Report
Wed 20 Mar 2013
8016-8-32.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-32.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5737366
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 13278 0.23143024168233295 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 13225 0.2305064728309123 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 12182 0.21232739901899234 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 12062 0.2102358469025682 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10635 0.1853638063180909 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10591 0.18459690387540206 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 10331 0.18006520762314973 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 10215 0.17804337391060635 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 10119 0.17637013221746703 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 10105 0.1761261178038842 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 9720 0.1694157214303567 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 9495 0.16549406121206142 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 9435 0.16444828515384935 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 9370 0.16331536109078626 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 9346 0.16289705066750143 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 9264 0.16146782338794494 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 9185 0.16009088491129903 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 9078 0.1582259176074875 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 9033 0.15744158556382842 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 8981 0.15653524631337795 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 8884 0.15484457501926843 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 8780 0.1530318965183675 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 8421 0.14677466977006523 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 8357 0.14565917530797232 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 8355 0.14562431610603194 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 8352 0.14557202730312133 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 8349 0.14551973850021074 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 8287 0.14443910324005824 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 8272 0.1441776592255052 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7440 0.12967623121829774 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 7400 0.1289790471794897 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7385 0.12871760316493666 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 7340 0.1279332711212776 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 7258 0.1265040438417211 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 7134 0.12434277332141613 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 6932 0.12082199392543545 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 6929 0.12076970512252486 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6851 0.11941019624684915 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6807 0.1186432938041603 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 6804 0.11859100500124971 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6799 0.1185038569963987 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6572 0.114547337576163 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6519 0.11362356872474233 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 6515 0.11355385032086152 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 6501 0.11330983590727871 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6465 0.11268237027235146 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 6456 0.11252550386361963 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 6311 0.10999821172294046 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6305 0.10989363411711925 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6301 0.10982391571323843 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6285 0.10954504209771522 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 6153 0.10724433476964866 No Hit
* 6071 0.10581510749009214 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 6068 0.10576281868718154 No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA 6061 0.10564081148039012 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 6014 0.10482162023479066 No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG 5855 0.10205031368052866 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5854 0.10203288407955846 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 5853 0.10201545447858826 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5846 0.10189344727179685 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 5800 0.1010916856271676 No Hit
TGAGACCGCACCAGCGACACA 5775 0.10065594560291255 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3025135 3.4604588 4.368015 60-64
AAAAA 3235020 3.4380634 6.9279413 2
GGGGG 821865 3.0656583 8.587871 4
GGAGG 967305 2.806916 5.193911 4
CCAGG 958625 2.6724758 5.585862 5
CCAGC 968465 2.646357 8.624322 5
CCTCC 969650 2.6355393 9.825973 6
CCCCA 963510 2.5805976 6.025041 1
CCTGG 910800 2.5767865 5.595827 9
CAGCC 930565 2.5427945 6.5489316 6
TGGGG 818160 2.409321 11.113515 3
CTCCC 855310 2.3247595 5.031545 3
GCTGG 770345 2.2235208 6.111386 7
GAGGG 743830 2.1584387 5.9296055 9
GGGGA 725020 2.1038558 5.409418 7
CCACC 780195 2.0896196 5.032563 1
CTCCT 922805 2.020217 5.776799 4
AAGAA 1451960 1.9835763 5.414778 2
CTCCA 908290 1.9593961 5.218607 6
TCCAG 878220 1.932865 6.040366 4
CCCAC 712755 1.9089931 6.632244 9
TGGCT 836745 1.9066991 6.6153293 3
GCCAG 683660 1.9059225 7.8129754 4
TGTTT 1294140 1.8751549 5.817465 3
CCCTG 673125 1.8665977 5.0911446 1
CACCA 876140 1.8624334 5.2299004 8
TGCAG 826805 1.8565292 5.9786286 3
GGCTC 643255 1.8198625 7.4431195 1
TCCTC 824210 1.8043716 6.6739197 5
GCCCA 655755 1.7918687 6.141497 4
AAACA 1321365 1.7693611 5.021781 2
AAAGA 1294230 1.7680953 5.1272335 2
AGGGG 596035 1.7295686 7.213935 6
CCAGA 772140 1.6745719 5.093198 8
GGCCC 459080 1.6125388 5.405267 1
GCTCC 557235 1.5452309 8.186909 2
TGCCC 556610 1.5434977 12.214443 3
TAAAA 1405395 1.5157436 8.5643 1
GATGG 659565 1.5109732 5.757933 1
ATGGA 830220 1.4795685 10.498762 2
CATGG 642885 1.4435505 42.98353 1
GGGGT 455705 1.3419619 5.1647944 4
GCAGT 590345 1.3255758 5.6120086 4
TGCTC 579560 1.294455 5.6827593 1
ATGTG 715485 1.2939957 7.959279 2
TGGGT 550850 1.2806277 6.355383 3
ATGAA 895575 1.241614 8.6679535 2
ATGGG 538500 1.2336298 15.643007 2
ATGGC 545425 1.2247113 11.036128 2
AGACA 709995 1.2220987 5.3055983 2
ATGCC 538340 1.1848267 12.147256 2
CATGT 612430 1.085646 33.15106 1
ATGTT 737840 1.0534832 8.179534 2
CATGA 598460 1.0453856 29.060974 1
TAAAG 745180 1.0331082 7.2461634 1
ATGCT 564495 1.0006723 8.004553 2
ATGGT 548140 0.99134266 7.6530557 2
ATGAC 557175 0.9732693 7.194789 2
CACTC 451015 0.9729458 5.54117 1
ATGAT 675280 0.9500773 6.5188556 2
ATGTA 669440 0.9418607 9.4314785 2
CATGC 415740 0.9149977 32.879753 1
TAAGA 587155 0.8140243 5.3209453 1
TAACT 550305 0.7588885 5.955355 1
TAAGT 536620 0.7549912 6.6321073 1
ATGCA 425505 0.7432691 9.149948 2
TAACA 507565 0.6897247 5.0450153 1
TAAAC 506455 0.6882164 5.778721 1
TAAGG 368165 0.6561216 5.406502 1
TGCCG 202440 0.57273245 6.710521 5
Produced by FastQC (version 0.10.0)