FastQCFastQC Report
Wed 20 Mar 2013
8016-8-33.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-33.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4219912
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 12985 0.3077078384572949 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 9257 0.21936476400455743 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 9198 0.2179666305837657 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 9016 0.21365374443827265 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 8989 0.21301392066943575 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8659 0.2051938523836516 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8611 0.2040563879057194 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 7012 0.16616460248460158 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 6967 0.1650982295365401 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 6898 0.1634631243495125 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6870 0.16279960340405203 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 6815 0.16149625868975465 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 6689 0.15851041443518255 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 6664 0.1579179850195928 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 6660 0.15782319631309846 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6499 0.15400795087670074 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 6466 0.15322594404812234 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6456 0.15298897228188643 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 6394 0.15151974733122398 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 6255 0.1482258397805452 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 6245 0.1479888680143093 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 6198 0.14687510071300067 No Hit
GAGACCGCACCAGCGACACC 6156 0.14587981929480995 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6092 0.1443631999909003 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6067 0.14377077057531057 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 6042 0.14317834115972086 No Hit
GGTGTCGCTGGTGCGGTCTC 5945 0.14087971502723282 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 5787 0.13713556112070585 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 5741 0.13604549099602078 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5641 0.13367577333366193 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5629 0.13339140721417886 No Hit
TGAGACCGCACCAGCGACACA 5626 0.1333203156843081 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 5579 0.13220654838299947 No Hit
TGTGTCGCTGGTGCGGTCTCA 5563 0.13182739355702205 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 5557 0.1316852104972805 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 5444 0.12900742953881503 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 5382 0.12753820458815254 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5369 0.1272301412920459 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 5368 0.1272064441154223 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5322 0.12611637399073725 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5305 0.12571352198813623 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5219 0.12367556479850764 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 5153 0.12211155114135082 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5128 0.1215191217257611 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5087 0.12054753748419397 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5053 0.11974183347899198 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4937 0.11699296099065572 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 4933 0.11689817228416137 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 4903 0.11618725698545372 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 4900 0.11611616545558297 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 4753 0.11263268049191548 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 4691 0.111163455541253 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 4690 0.11113975836462942 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 4678 0.11085539224514634 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 4651 0.11021556847630946 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 4605 0.1091254983516244 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 4486 0.10630553433341738 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 4473 0.10599747103731073 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 4453 0.10552352750483897 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 4437 0.10514437267886154 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 4341 0.10286944372299707 No Hit
CCCTCTCTTTATTTCTCAGTCTGACTTTCAGCTTGTCCCAGCTACTGTAA 4331 0.10263247195676117 No Hit
CCGCTAGTCTAACAGACAGTCACAACCAGTGCTATGGAAAACCAAAGATA 4279 0.1014002187723346 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 4269 0.1011632470060987 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 4241 0.10049972606063823 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2502025 3.6306162 7.1171484 2
TTTTT 2202965 3.4842348 4.3277082 60-64
GGGGG 628180 3.2256262 7.9663715 4
GGAGG 696720 2.778561 5.37108 4
CCAGC 696745 2.64649 8.597293 5
CCAGG 685175 2.6451664 5.388702 5
CCTCC 686320 2.6094573 9.563009 6
CCTGG 656510 2.5785472 6.8279448 9
CAGCC 669465 2.5428708 6.984526 6
CCCCA 678780 2.5367072 5.736174 1
TGGGG 595185 2.414882 10.346144 3
CTCCC 608915 2.3151557 5.062361 3
GCTGG 566555 2.2616787 8.4660225 7
GAGGG 534845 2.132994 6.4750605 9
GGGGA 529175 2.1103816 5.5609236 7
CCACC 554660 2.0728514 5.0383677 1
CTCCT 661960 2.02127 5.760716 4
CTCCA 662365 1.9879606 5.6355934 6
AAGAA 1059095 1.9787544 5.2303925 2
TCCAG 634995 1.9370273 6.352448 4
CACAC 651480 1.921893 5.1514454 8
TGGCT 608965 1.9208574 7.1700954 3
CCCAC 507510 1.8966442 6.38085 9
CACCA 638880 1.8847225 6.6359687 8
GCCAG 487450 1.8818352 7.3817234 4
TGGTG 581200 1.8633032 5.8508763 9
GGCTC 472825 1.8570952 7.61365 4
TGTTT 925865 1.8532479 5.5906186 3
TGCAG 595365 1.8458817 6.0342593 3
TCCTC 598860 1.8285965 6.5875483 5
CAGTG 588700 1.8252175 6.749221 1
GCCCA 467145 1.774386 6.0564165 4
AGGGG 437245 1.7437594 7.394274 6
GGCCC 328800 1.6080385 5.1490903 1
TGCCC 397775 1.5371494 11.140331 3
TAAAA 1037100 1.5310577 8.6974325 1
GCTCC 395450 1.5281647 7.4260225 2
CTGGT 481790 1.5197095 5.305173 6
ATGGA 608025 1.4880888 10.539266 2
GATGG 470905 1.4839149 6.099504 1
CATGG 466165 1.4453075 41.363697 1
GCAGT 426975 1.323802 5.662703 4
TGCTC 420765 1.305832 5.4319425 1
ATGTG 516010 1.284836 7.810054 2
TGGGT 399510 1.2808125 6.223339 3
ATGAA 659805 1.2541652 9.35818 2
TGAGA 499510 1.2225077 5.400102 1
ATGGC 393745 1.2207749 11.058448 2
ATGGG 385380 1.2144086 14.903493 2
ATGCC 392715 1.1979617 10.962083 2
GTCTT 458660 1.1431615 6.061366 9
GAGAC 365815 1.1148075 5.5052524 2
AACAC 473875 1.1035165 5.2536774 2
CATGT 434735 1.0650234 31.876554 1
CATGA 441980 1.0642778 29.866358 1
ATGTT 529120 1.0410168 7.6698384 2
TAAAG 534625 1.0162216 7.0000424 1
ATGCT 412275 1.0100003 7.9006133 2
GTGTC 315750 0.9959698 5.410859 2
CACTC 329425 0.98870546 5.7802415 1
AGACT 406110 0.9779036 5.0337434 1
AGACC 324495 0.97295177 5.0554976 3
ATGGT 389520 0.96988314 7.0834036 2
ATGAT 498615 0.9642435 6.9181237 2
ATGAC 400120 0.96347964 6.9103603 2
GCGCC 187940 0.91914463 5.961352 8
ATGTA 474315 0.91725105 9.011895 2
CATGC 298300 0.9099525 31.779774 1
CGCTG 212805 0.8358254 6.167667 6
TAAGT 394430 0.76276594 6.866064 1
TAACT 390935 0.7438252 5.719512 1
ATGCA 305320 0.73520344 9.055301 2
TAAAC 372765 0.6971387 5.4532666 1
TAAGG 264350 0.64697385 5.03361 1
TGCCG 147550 0.579526 8.8208475 5
CCGTC 126620 0.48930642 6.275791 7
GCCGT 114745 0.45067918 6.362225 6
CGTCT 144150 0.44736534 5.5759726 8
GTCGC 91700 0.3601663 5.5406713 4
Produced by FastQC (version 0.10.0)