FastQCFastQC Report
Wed 20 Mar 2013
8016-8-34.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-34.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3873226
Filtered Sequences 0
Sequence length 1-100
%GC 46

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 9489 0.24498957716384226 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 9397 0.2426142961965039 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8096 0.20902472512577372 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 8092 0.20892145204023724 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 8053 0.2079145394562569 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7950 0.2052552575036933 No Hit
* 6965 0.17982426019034262 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6894 0.17799116292207065 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6869 0.17734570613746783 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 6722 0.17355042024400333 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 6679 0.17244023457448648 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6602 0.17045222767790982 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 6599 0.1703747728637575 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 6586 0.17003913533576404 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6569 0.16960022472223413 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6287 0.16231947219191445 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6198 0.16002164603872843 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 6067 0.1566394524874097 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 6014 0.15527108410405177 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 6010 0.15516781101851532 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 5978 0.1543416263342237 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 5950 0.1536187147354686 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 5888 0.1520179819096536 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 5835 0.15064961352629566 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 5781 0.14925542687155358 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 5698 0.14711251034667225 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 5609 0.14481468419348625 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 5515 0.1423877666833797 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 5491 0.141768128170161 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 5449 0.14068376077202827 No Hit
CGAGACCGCGCCCCAGGCCCAGCACTCACAAATTCCTGGTCGTGGTTATT 5425 0.14006412225880957 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 5361 0.1384117528902264 No Hit
GGCACAAGGGTACCTACGGGCTGCTGCGGCGGCGAGAGGACTGGCCCTCC 5343 0.13794702400531236 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 5267 0.13598483538011982 No Hit
TGCTCTGTGGTCCGAGGCGAGCCGTGAAGTTGCGTGTGCGTGGCAGTGTG 5248 0.1354942882238217 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 5241 0.13531356032413291 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 5234 0.13513283242444413 No Hit
CCGCGGCGCAGATCGAAACAGATCGGGCGGCTCGGGTTACACACGCACGC 5183 0.13381610058385437 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 5034 0.12996917814762163 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 5007 0.1292720848202506 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4907 0.12669025768183936 No Hit
CGAGGCGAGCCGTGAAGTTGCGTGTGCGTGGCAGTGTGCGTGGCAGGATG 4553 0.1175505896118636 No Hit
TCGTCTTCTGGGATGGCAGCTTTGCTTCAAGCGTCTAGTCTGTTAAAAGG 4549 0.11744731652632714 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 4525 0.11682767801310845 No Hit
GAGGCACGAACACCCACGAGCGCCGCGTAACCGCAGCAGGTGGAGCGGGC 4522 0.11675022319895612 No Hit
AGGGTCAGCCCACACCACCCAGCCCCCACGCCCAACCCTCGTGCCTCCCT 4509 0.11641458567096265 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 4500 0.11618222122850563 No Hit
CATGCCCCTCCGCTGTCCCTTCGAGTCAGGTGTCTGTCCTTGGCTCCCAG 4480 0.11566585580082339 No Hit
CATGGGGGCCAGTTGTGGGTGGTGGCCCAGGTGCAGGAGAGGCGGGCAGT 4474 0.11551094617251872 No Hit
CGCAGTGACGCCCCCAATCTGCGCGCGGGACCAGCACACCAACTTTATCG 4467 0.11533021827282994 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 4459 0.11512367210175704 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 4371 0.11285166421995516 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 4315 0.11140584102244486 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 4302 0.11107020349445139 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 4300 0.11101856695168318 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 4259 0.10996001782493457 No Hit
GGCTCCGCAGCCCCTGCCGCCGCCGCCGCCGCCTTCACCGCCGCCGCGTT 4197 0.10835928499911959 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 4130 0.10662946081638407 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 4103 0.10593236748901304 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 4031 0.10407345194935694 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 4026 0.10394436059243638 No Hit
GCCGCTCACGGCACAGCCCAGTAAAGATAAGAGGCCTTCCATCACCGGTA 4022 0.10384108750689992 No Hit
TGAGACCCGAAGACAGGGCCCACACCTCCAGCCCTTCTAAAACATAGTTA 4017 0.10371199614997936 No Hit
CAGCAGTCCCAGCAGCCCCAGCCTCTACAGAAGCAGCCACCACAGCCCCA 4015 0.10366035960721115 No Hit
CATGATATCTCGAGCCAATCCAAAGTCACATATCTTCACCACTTTCCCGT 3999 0.10324726726506535 No Hit
GCTGCTACGTCCCTAATCCCCCTTGTTGGGAGGATTTTCGTATCACCCTT 3984 0.10285999319430367 No Hit
GCAGAGCAGCTAGTCATTCAGCAGAGGCCGGCCAGAGAGGGCCCGGCTCA 3966 0.10239526430938964 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 3963 0.1023178094952373 No Hit
CATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAA 3962 0.10229199122385318 No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC 3936 0.10162071616786628 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 3923 0.10128507863987281 No Hit
AGCACCTCACGGCCCCCACAGCAGATGGTGCAGTAGGACTGGTAGCCGTC 3904 0.10079453148357467 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 3890 0.1004330756841971 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1882720 3.6812599 7.101238 2
TTTTT 1709330 3.5964258 4.4466004 60-64
CCCAG 867515 2.9791934 5.3026333 2
GGGGG 639830 2.7614849 10.386233 4
GGAGG 707470 2.6062295 5.4333496 4
CCAGC 750755 2.57822 6.831231 5
CAGCC 750150 2.576142 5.640651 6
CCTCC 751515 2.5583813 9.311361 6
CCCCA 759370 2.5475001 5.849427 1
AGCAG 776310 2.3845444 5.161575 5
TGGGG 630575 2.357264 11.660982 3
GGGAG 624510 2.3006153 6.0662794 6
CTCCC 652360 2.2208283 6.3441577 3
CTCCT 674010 2.0344565 5.6477184 4
AAGAA 888090 2.0344226 5.400955 2
CTCCA 678840 2.019215 6.40721 6
TCCAG 655915 1.9972097 6.041083 7
GGGGA 538735 1.9846312 6.324607 5
CCCTC 571005 1.9438715 5.585393 5
TGCAG 619030 1.9295177 5.3005476 3
CCCAC 572065 1.9191377 5.242434 9
TGGCT 599820 1.8972515 8.078585 3
TGTTT 781030 1.8972279 5.329201 3
GGCTC 527930 1.8833277 10.870295 1
GCCCA 543215 1.8654925 7.1888695 2
TCCTC 605475 1.8275878 6.2623076 5
GCCAG 515420 1.8119407 6.359259 4
AGCCC 523725 1.7985604 5.838681 1
TAAAA 832255 1.6513307 9.060682 1
GGCCC 407890 1.6411122 7.0096855 1
AGGGG 441950 1.6280873 6.8527436 6
GCTCC 459655 1.6018454 11.546405 2
TGCCC 452370 1.5764581 13.836899 3
CCTCA 505730 1.504298 5.0190506 6
ACAGC 496835 1.4908068 5.0518494 7
GATGG 463475 1.4788506 5.6589413 1
CTCAG 471270 1.4349802 6.01726 3
ATGGA 525630 1.431544 10.649293 2
CATGG 456445 1.4227399 41.40816 1
GCTCT 433590 1.3397453 5.8375225 2
TGCTC 426860 1.3189504 8.082849 1
CAACA 511990 1.3112853 5.131725 5
TGGGT 402755 1.3040848 6.105178 3
GCACA 425615 1.2771037 5.0802402 2
ATGGG 399565 1.2749274 14.683633 2
ATGTG 460590 1.2729343 9.340219 2
AACTG 471960 1.2556506 5.0563025 4
AGCAC 417910 1.2539841 5.627268 1
CTCAC 416675 1.2394031 5.263584 7
ATGAA 531455 1.2354285 8.684679 2
ATGGC 390890 1.2184049 12.458804 2
TGAGA 439030 1.1956904 5.3823986 1
ATGCC 385390 1.1734823 14.411993 2
GGCAC 332330 1.1682943 6.405577 1
GGAGT 363450 1.1596922 6.3140845 7
GAGAC 354445 1.088727 6.0383196 2
GAGTG 340595 1.0867665 5.354173 8
ATGTT 449995 1.0771908 7.3957424 2
CATGT 389445 1.0514208 32.301083 1
CATGA 390190 1.0381013 27.395935 1
TAAAG 444070 1.0322919 6.7347856 1
ATGCT 372565 1.0058484 9.059918 2
ATGAG 365525 0.9955008 5.455664 2
AGACT 373370 0.99335176 5.001872 1
ATGAT 412090 0.972098 6.5312033 2
ATGGT 348140 0.9621557 7.3717504 2
AGACC 318295 0.95507854 5.1264477 3
ATGAC 356480 0.94841576 6.7075434 2
ACAAC 370285 0.94835705 5.31978 4
CATGC 310930 0.94675744 34.273617 1
ATGTA 394905 0.9315595 9.36712 2
TAAGA 361210 0.8396743 5.6639104 1
TAATG 328990 0.7760696 5.6957574 1
TAAGT 322075 0.7597575 7.380899 1
ATGCA 282675 0.75205743 9.316999 2
ATGTC 274050 0.7398783 5.5582633 2
TAACT 318960 0.73500997 5.2518196 1
TAAAC 298960 0.67889595 5.752553 1
TGCCG 170360 0.60773915 7.214542 5
Produced by FastQC (version 0.10.0)