FastQCFastQC Report
Wed 20 Mar 2013
8016-8-35.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-35.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5020740
Filtered Sequences 0
Sequence length 1-100
%GC 41

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGTGTCGCTGGTGCGGTCTCACTGCAGCCTGCAGAGCCACA 20364 0.40559758123304535 No Hit
TGTGGCTCTGCAGGCTGCAGTGAGACCGCACCAGCGACACA 19818 0.3947226902807156 No Hit
TGTGTCGCTGGTGCGGTCTCAGCTTGGCGCAATCATGACACA 14739 0.29356230356481317 No Hit
TGTGTCATGATTGCGCCAAGCTGAGACCGCACCAGCGACACA 14457 0.2879456016443791 No Hit
TGTGTCGCTGGTGCGGTCTCACGAGACTGCCCTTAACCTACACA 13124 0.26139573050984516 No Hit
TGTGTAGGTTAAGGGCAGTCTCGTGAGACCGCACCAGCGACACA 12927 0.25747200611862 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 12584 0.2506403438536949 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 12573 0.25042125264403253 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 11978 0.23857040993957068 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 11917 0.2373554495950796 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 9100 0.18124818253882893 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 9045 0.18015272649051733 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 8362 0.16654915410875687 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 8345 0.16621055860291511 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 8300 0.16531427638156926 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 8238 0.16407939865438162 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 8199 0.1633026207292152 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 8102 0.16137063460764747 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 8041 0.1601556742631564 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 7685 0.15306508602317587 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 7626 0.151889960444078 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 7568 0.15073475224767663 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 7351 0.14641268020251996 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 7301 0.14541681106769122 No Hit
* 7254 0.1444806940809522 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 7009 0.13960093532029144 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7004 0.13950134840680856 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 6783 0.13509960683086558 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 6735 0.13414357246143 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6695 0.133346877153567 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT 6682 0.13308795117851152 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6663 0.1327095209072766 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6626 0.13197257774750334 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 6580 0.13105637814346094 No Hit
ATACTTGTAATTCCCCTAACCATAAGATTTACTGCTGCTGTGGATATCTC 6513 0.12972191350279044 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 6512 0.12970199612009384 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 6394 0.12735174496189805 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 6386 0.12719240590032546 No Hit
CATGCTTTACTCAACTGAAATAATCAAGCCCCTATTGCTGTGGGGGAAGC 6362 0.12671438871560767 No Hit
CATGACTATAAATATGAAGCAAATATGATTATCCTCACAAGTTCTGGAAT 6304 0.12555918051920634 No Hit
AACCTGACAGAAAAACAAAACTCTTCATATTGGTCAATTTCATAATCTTC 6296 0.12539984145763372 No Hit
TAACTAACTCATAAATGCCTATTTTTTCTTACAGAAAAGGGATTTATAAA 6253 0.12454339400168103 No Hit
GTATCTTAATGATGTATATAATTGCCTTCAATCCCCTTCTCACCCCACCC 6249 0.12446372447089474 No Hit
ACTTGAAAGCAAGGATAGAGAAACATTTTAGAGATATCTGGGTTTTTTTC 6109 0.1216752908933743 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 5820 0.11591916729406422 No Hit
CATGACTATAAATATCAAGCAAATATGATTTTACTCACAAGTTCTGGAAT 5785 0.11522205889968411 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATCTGGGTTTTTTTC 5784 0.11520214151698753 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 5725 0.11402701593788962 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 5700 0.11352908137047527 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 5689 0.11330999016081296 No Hit
TGATCCCTTTTTCCTTTTCTCTTTCCTTGACTCCCGCTTATTCTCCTTTT 5618 0.11189585598935615 No Hit
GACTGAAAGAGGGAGAAATAAAGACAAGGCCCCCGAGGAGCTGTCCAAAG 5571 0.11095973900261714 No Hit
ATGTCAGATATAAAGAACTCACCGAACAGCAGCTCCCAGGCGCACTTCCT 5568 0.11089998685452741 No Hit
TAAAACATCGCCTACAGAAAAGCGTATGAAAGGAGTGTAAGCTTTGCTCT 5548 0.11050163920059593 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 5522 0.10998378725048498 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 5509 0.1097248612754295 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 5441 0.10837047925206245 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 5429 0.10813147065970355 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 5426 0.10807171851161383 No Hit
CAATTCAAATAAAAACGAAACTGTGTCAATTAGTTGAAGTAATGATGGCA 5411 0.1077729577711652 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 5411 0.1077729577711652 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 5379 0.10713560152487481 No Hit
CTGAGGAAATAAGATGTAGGGCATTTCTGATGTGACTCAGTGGGAAAACT 5339 0.10633890621701184 No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG 5326 0.10607998024195636 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 5303 0.10562188043993515 No Hit
TAATTATGGAATATTTACCATATGGAAGTTTACGAGACTATCTTCAAAAA 5292 0.10540278923027284 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 5283 0.10522353278600366 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5239 0.10434716794735437 No Hit
CATGTCCTCCTTTCTACCAATAACCGCATATCTACTCTTTTTCTGTACTT 5172 0.10301270330668388 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5165 0.10287328162780786 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 530770 3.2602842 6.2504597 75-79
CCAGC 731300 3.0203676 10.009433 30-34
TTTTT 2832085 3.0175087 4.0003605 20-24
AAAAA 3071980 2.9962595 7.005721 2
GCTGG 631350 2.705674 58.344982 7
CCTGG 592945 2.516734 5.610352 95-96
GGCTG 567765 2.4331782 5.051453 6
CCTCC 578625 2.4091024 7.1347656 6
GGCAG 544480 2.2925086 5.667674 8
ACACA 1134980 2.2670424 6.188793 35-39
TGTGT 1051915 2.258633 38.432938 1
TGGTG 736420 2.244667 38.736435 9
CACCA 783010 2.2381744 6.5834365 30-34
TGGGG 514610 2.2267215 7.544442 3
TGCAG 734455 2.1783762 9.104415 9
CTGCA 734510 2.15766 9.056658 8
GGTGG 495505 2.144054 5.0102806 7
GAGGG 494230 2.1010702 8.322286 9
TGTGG 670550 2.04389 11.333105 1
GGGGA 478235 2.0330722 5.4082747 7
CTGGA 677455 2.0093155 5.0747 8
TGGCT 655055 1.9775238 11.138192 3
ACCAG 681655 1.967314 7.6507096 30-34
CTCTG 656060 1.9615759 8.929293 6
TCTGC 655265 1.9591991 9.164306 7
GCCAG 469560 1.9581128 5.419646 4
CCCAC 459925 1.8813467 6.8284535 9
GAGGA 637685 1.8762039 5.2432027 1
AAGAA 1324195 1.8661671 5.969845 2
CTGGT 617285 1.863501 39.355335 8
GTGGC 424515 1.8192749 15.184825 2
TCCAG 610600 1.793668 5.4174953 4
TGTTT 1155395 1.747568 5.89679 3
GCCCC 292130 1.7266152 5.6262217 9
AGGGG 400345 1.7019463 6.167064 6
GGCTC 397675 1.6879174 14.606195 4
AAAGA 1197625 1.6877942 5.65385 2
GGTGC 391845 1.6792663 11.1772 10-14
GCACC 396135 1.6360909 6.419612 30-34
GAGAC 543660 1.5842321 6.966512 20-24
GAAAG 774145 1.5763716 6.0396814 5
GTGTC 520625 1.5716976 48.599102 2
TCTCA 740375 1.5320597 6.256619 15-19
ATGGA 734545 1.5224074 9.82133 2
TGAGA 731430 1.5159514 5.2356544 20-24
AGCAA 748855 1.5102594 5.2062073 8
TGCCC 359210 1.5100416 6.774322 3
GTCTC 501965 1.500842 9.159305 15-19
GCAGT 496290 1.4719845 5.1471443 4
GACAC 501715 1.447992 8.071439 35-39
CATGG 477230 1.4154528 37.91233 1
TGGCC 333105 1.4138523 6.1423955 3
CTCAC 484230 1.408817 5.141273 15-19
GCTCT 468145 1.3997225 8.466453 5
TAAAA 1382105 1.3720765 8.620794 1
GGTCT 452565 1.3662335 8.345621 15-19
ATGTG 644625 1.3598653 6.683509 2
AGACC 456175 1.3165597 5.167492 20-24
CAGCG 309690 1.2914388 7.416651 30-34
TGTCA 615765 1.2865343 6.2567115 3
TGTCC 425620 1.2725755 5.443427 3
ATGAA 862790 1.2375991 7.531019 2
CGCTG 288725 1.225483 52.25904 6
TGGGT 399955 1.2190948 5.553382 3
CATGA 585140 1.2011296 29.883947 1
ATGCC 400955 1.1778255 7.8027883 2
ATGGC 396580 1.1762469 6.994227 2
CATGT 558425 1.1667323 34.680748 1
TGACT 549550 1.1481895 5.1153855 3
AACAC 571795 1.1421201 5.7955055 2
GCAAG 376420 1.0968926 5.219822 9
ATGGG 361420 1.0823364 11.868135 2
ATGCT 513620 1.07312 7.4872103 2
ATGAC 517520 1.0623245 7.9798317 2
TGTAG 500455 1.0557323 10.567803 3
ATGGT 496130 1.0466084 6.35495 2
CGCAC 253190 1.0457088 7.346401 25-29
ATGTT 699075 1.038846 8.992492 2
GTGCG 241780 1.0361573 11.257267 10-14
TAAAG 713110 1.0228958 7.8927445 1
ATGAT 694815 1.0144259 6.406167 2
TGCGG 236495 1.0135082 11.8081 10-14
CCGCA 244285 1.0089298 8.829867 25-29
GCGGT 234725 1.0059228 11.623334 10-14
CGGTC 233555 0.99131584 11.310259 10-14
GTAGG 328925 0.9850244 10.993397 4
ATGTA 673920 0.9839193 9.26163 2
GTCAT 460880 0.9629289 5.8780217 4
GACCG 224130 0.9346449 8.121881 25-29
ACCGC 224680 0.92795867 9.007749 25-29
CATGC 315305 0.92622423 28.615067 1
AGGTT 435405 0.9185063 5.612964 6
TCATG 435950 0.910842 5.2142715 5
GTCGC 198895 0.8442027 51.874424 4
GCGAC 198915 0.8294958 7.81909 35-39
ATGCA 384570 0.7894151 7.6886034 2
TAAGA 543485 0.77958316 5.5612645 1
TCGCT 253770 0.75875545 36.610275 5
TAATG 511525 0.74682355 5.1872616 1
TAACT 515485 0.745392 6.2981386 1
TAAGT 508715 0.74272096 6.7283425 1
GATTG 351365 0.74122024 5.128564 9
GTGTA 341255 0.7198927 6.640078 2
TAAGG 340265 0.7052283 5.3523393 1
TGTCG 228910 0.6910488 37.088554 3
TAAAC 485680 0.6899898 5.757257 1
TAGGT 324740 0.6850535 6.236214 5
CGACA 236870 0.68362683 6.7173624 35-39
TAACA 468225 0.66519207 5.269483 1
GGTTA 271515 0.57277304 5.955403 7
TGCCG 115145 0.48872882 6.620729 5
GCCGT 95445 0.4051129 5.6590824 6
CCGTC 88500 0.37203497 5.6097956 7
Produced by FastQC (version 0.10.0)