FastQCFastQC Report
Wed 20 Mar 2013
8016-8-36.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-36.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4157334
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 17824 0.42873630071579527 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 11480 0.276138506071439 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11427 0.274863650599158 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 8407 0.2022209425559746 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 8370 0.2013309491130614 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 8360 0.20109041034470648 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 8280 0.1991661001978672 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 7511 0.18066866891137445 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 7489 0.18013948362099363 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 7471 0.1797065138379548 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 7069 0.17003685535008733 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6684 0.1607761127684232 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 6644 0.1598139576950036 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6642 0.15976584994133258 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6640 0.1597177421876616 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 6577 0.15820234794702565 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 6573 0.1581061324396837 No Hit
ATACTTGTAATTCCCCTAACCATAAGATTTACTGCTGCTGTGGATATCTC 6499 0.15632614555385735 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 6430 0.15466642805220845 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 6417 0.1543537276533471 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6383 0.15353589584094035 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6364 0.15307887218106606 No Hit
CATGCCCACAGAGACTTGCACAACATGCAGAATGGCAGCACATTGGTAAG 6302 0.15158753181726559 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6178 0.14860485108966467 No Hit
CTGAGGAAATAAGATGTAGGGCATTTCTGATGTGACTCAGTGGGAAAACT 6075 0.14612730177560906 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 5997 0.14425109938244077 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 5902 0.1419659810830691 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 5848 0.14066707173395257 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 5788 0.1392238391238231 No Hit
CGAGACCGCGCCCCAGGCCCAGCACTCACAAATTCCTGGTCGTGGTTATT 5671 0.13640953553407065 No Hit
GGCACAAGGGTACCTACGGGCTGCTGCGGCGGCGAGAGGACTGGCCCTCC 5601 0.13472576415558624 No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA 5304 0.12758176273544536 No Hit
AGTTCTAACATTTTGTTTATGGCTTTTTGGGGGGTTTTCATTGCAGGATG 5290 0.12724500845974848 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 5283 0.12707663132190003 No Hit
TAAGCAATGCCACAGGGGCATGATATCCTGGCTTCTTCTGACCAAGCAAA 5248 0.12623474563265785 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 5224 0.12565745258860608 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 5195 0.12495989016037681 No Hit
AACCTGACAGAAAAACAAAACTCTTCATATTGGTCAATTTCATAATCTTC 5185 0.12471935139202191 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 5184 0.12469529751518642 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 5166 0.12426232773214757 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 5159 0.12409395059429915 No Hit
TAACTAACTCATAAATGCCTATTTTTTCTTACAGAAAAGGGATTTATAAA 5156 0.12402178896379265 No Hit
AGAACAGTTGCAAATAGACTACATAATATACGTGGGCAAAAAGGCAATTA 5126 0.12330017265872793 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 5114 0.12301152613670202 No Hit
TAATTGCCTTTTTGCCCACGTATATTATGTAGTCTATTTGCAACTGTTCT 5085 0.12231396370847279 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 4899 0.11783994261707142 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 4867 0.11707021855833571 No Hit
TAATAGGGTGGTTCTCTTCCCAAAGTGGAAGCCAAATTCATCAATTATGT 4843 0.11649292551428392 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 4840 0.11642076388377744 No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG 4821 0.1159637402239031 No Hit
ATCATGTCCCCAGAAAATCCAGTTACCTCTTGGTCAGTCATCCGGAAGCA 4821 0.1159637402239031 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 4792 0.11526617779567387 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 4772 0.11478510025896405 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 4680 0.11257214359009883 No Hit
TAAACATGTCTGCAGAAGAGCAGCTGTTGCCCTTGGCCGAAAACGAGCTG 4642 0.11165809627035019 No Hit
ATCCCCTCTTGCCATCTGTTTTTCAGAGGACCTACTAACTATGAAGAGCC 4587 0.11033513304439817 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 4562 0.1097337861235109 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 4550 0.10944513960148498 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 4528 0.10891595431110418 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 4485 0.10788163760717807 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 4466 0.10742461394730374 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 4401 0.1058611119529968 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 4381 0.10538003441628697 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 4377 0.10528381890894502 No Hit
CCGCGGCGCAGATCGAAACAGATCGGGCGGCTCGGGTTACACACGCACGC 4355 0.10475463361856421 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 4355 0.10475463361856421 No Hit
TGCTCTGTGGTCCGAGGCGAGCCGTGAAGTTGCGTGTGCGTGGCAGTGTG 4343 0.1044659870965383 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 4325 0.10403301731349947 No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA 4323 0.10398490955982849 No Hit
AGAAAATTTACTTTGGAAACCTACTTTTCACAGAAGTCATTCAGAACACC 4237 0.10191627615197625 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 620945 3.63083 11.292872 4
AAAAA 2517350 3.299558 7.8075 2
TTTTT 2217525 3.209221 4.2772837 25-29
CCTCC 633235 2.6145751 7.3894258 6
CCCCA 639735 2.5895991 6.8215528 8
CCAGC 624600 2.5721276 5.581149 5
CAGCC 595835 2.4536722 5.0484657 8
TGGGG 535650 2.3689156 11.712188 3
GGGAG 537965 2.3324842 6.6217666 6
CTCCC 548580 2.2650416 8.79263 3
GCTGG 503360 2.1882107 5.0362024 3
GAGGG 492180 2.1339717 6.5202694 8
GGTGG 481545 2.1296358 6.704542 7
GGGGA 476995 2.0681334 7.0648704 5
CCCAC 493265 1.9966998 8.4171715 9
AAGAA 1103980 1.9514685 6.8238053 2
TGCAG 601825 1.9399589 5.4534802 3
TCCAG 611815 1.9385828 5.9917927 4
GCCAG 457615 1.917118 5.599472 4
GCCCA 459705 1.8930835 9.603066 2
AGAAG 791615 1.8871329 5.210642 3
GGCCC 328410 1.8238748 10.677699 1
TGTTT 942045 1.802549 5.4718213 3
CACCA 589725 1.8007437 5.1458035 8
GGCTC 415460 1.7753401 14.251048 1
AGCTG 550330 1.7739667 5.119624 9
CAGCT 558020 1.7681291 5.372345 8
AAACA 1012270 1.7588898 6.0929527 2
AGCCC 425195 1.7509699 6.779008 1
AGGGG 399730 1.7331313 5.128364 6
AAAGA 958180 1.6937426 5.7263975 2
CTTGG 504300 1.6581165 5.28929 1
TGCCC 374200 1.571803 10.311078 3
AAAAC 889855 1.5461851 5.626456 2
GCTCC 366370 1.5389136 14.778426 2
AAGGA 635150 1.5141356 5.153684 2
ACAGC 486695 1.5118799 5.8637614 7
GGGTG 336895 1.4899201 6.65685 6
CATGG 455510 1.4683181 34.023933 1
ATGGA 597635 1.4532096 8.597275 2
TAAAA 1077210 1.4401785 11.574384 1
GCAGT 416755 1.3433931 5.113638 4
ATGTG 537945 1.3342398 6.2045135 2
GCACA 428260 1.3303565 6.5162745 2
GCTCT 407195 1.3160441 8.34243 2
AGCAC 415260 1.2899727 5.1311774 1
TGCTC 398655 1.2884431 10.548401 1
TGGGT 378225 1.2651277 5.201215 3
ATGGC 390295 1.2581005 8.189052 2
ATGAA 688685 1.2417231 6.4821796 2
GGCAC 293905 1.2312764 8.15086 1
ATGGG 366345 1.2013531 11.915547 2
ATGCC 378215 1.1984034 10.208264 2
GGAGT 356165 1.1679697 6.1209245 7
GTCTT 453885 1.1287234 5.006685 9
CATGT 453600 1.1058875 27.384413 1
AACAC 471280 1.0855538 6.014688 2
ATGTT 563850 1.057731 6.847009 2
CATGA 433670 1.0365577 23.32585 1
ATGCT 420500 1.025189 6.5272455 2
ATGGT 413090 1.0245677 5.7838287 2
TAAAG 565095 1.018886 9.85145 1
ATGAC 419520 1.0027364 5.7877326 2
TAAAT 728190 0.9930348 5.227217 1
ACAAC 425930 0.9810938 5.2409563 4
CATGC 307460 0.9742105 27.774267 1
ATGTA 524855 0.9652665 7.594225 2
ATGAT 514565 0.946342 5.045295 2
CGCCC 163840 0.894418 6.227094 9
GCGCC 154225 0.85651195 5.4264507 8
TAAGA 444205 0.8009171 7.24749 1
TAATT 573305 0.79746056 7.1627398 1
ATGCA 331940 0.79340297 7.217868 2
TAACT 417430 0.754629 8.400087 1
TAAGT 407300 0.7490698 9.434961 1
TAATG 405160 0.7451341 6.940057 1
TAAAC 393845 0.69802564 8.045956 1
TAACA 382900 0.6786274 7.2270756 1
TAAGG 270235 0.6571036 7.18414 1
TAATC 319170 0.5769947 5.78332 1
TAACC 236225 0.55501145 5.3605995 1
TGCCG 126400 0.54013145 6.8888326 5
Produced by FastQC (version 0.10.0)