FastQCFastQC Report
Wed 20 Mar 2013
8016-8-38.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-38.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4752932
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 11941 0.25123439594759617 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10021 0.21083827835113148 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 9993 0.21024916830284968 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 9442 0.1986563241384476 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 9327 0.19623676501157603 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8392 0.176564697327881 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 8350 0.17568103225545834 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8315 0.1749446446951061 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 8208 0.17269340272488645 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 8198 0.1724830062790715 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 7968 0.16764388802532837 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 7734 0.16272061119325923 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7702 0.1620473425666515 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 7655 0.16105847927132136 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7609 0.16009065562057273 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 7275 0.15306341433035442 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 7237 0.1522639078362577 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7160 0.1506438552034828 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 7114 0.14967603155273418 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 7068 0.14870820790198555 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 6961 0.1464569659317659 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 6942 0.14605721268471755 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 6898 0.14513146832313192 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 6829 0.14367973284700894 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 6746 0.14193344234674513 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 6736 0.1417230459009302 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 6719 0.14136537194304483 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 6702 0.14100769798515947 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6667 0.14027131042480726 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6662 0.1401661122018998 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6637 0.1396401210873625 No Hit
GATCCAAAACTTCTCTCCGACACTGAATACCTTCTGCTTCTCCTATCATT 6542 0.13764135485212076 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6528 0.13734679982797987 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6447 0.135642588616879 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 6357 0.13374902060454474 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6339 0.1333703070020779 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 6241 0.13130842183309166 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6165 0.12970940884489826 No Hit
TAAACATGTCTGCAGAAGAGCAGCTGTTGCCCTTGGCCGAAAACGAGCTG 6073 0.127773761543401 No Hit
ATCCCCTCTTGCCATCTGTTTTTCAGAGGACCTACTAACTATGAAGAGCC 6071 0.12773168225423803 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5991 0.12604851068771866 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 5968 0.12556459886234433 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 5942 0.12501756810322553 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 5936 0.12489133023573659 No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA 5903 0.12419702196454735 No Hit
TGATCCCTTTTTCCTTTTCTCTTTCCTTGACTCCCGCTTATTCTCCTTTT 5877 0.12364999120542856 No Hit
GACTGAAAGAGGGAGAAATAAAGACAAGGCCCCCGAGGAGCTGTCCAAAG 5870 0.12350271369335813 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5776 0.12152498710269787 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5717 0.12028364807238985 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5656 0.11900022975291882 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5654 0.11895815046375584 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5549 0.1167489877826992 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5529 0.11632819489106935 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5513 0.11599156057776547 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5450 0.11466606296913148 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 5362 0.11281457424596017 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5326 0.11205714704102647 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 5319 0.11190986952895603 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5266 0.11079476836613694 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 5228 0.10999526187204026 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 5190 0.10919575537794354 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 5134 0.10801753528138 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 5114 0.10759674238975017 No Hit
AGAAGAGGGCTCCTTTTGTGCATATCAAACCTTGTTCCAGAATGGAGTGG 5074 0.10675515660649047 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 5059 0.1064395619377681 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 5053 0.10631332407027914 No Hit
GACCCCCTCCACTAACTCCCGAGGACGTTGGCTTTGCATCTGGTTTTTCT 5039 0.10601876904613824 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 5023 0.10568213473283439 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 5018 0.10557693650992692 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4984 0.1048615885941562 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTA 4955 0.10425143890129293 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 4955 0.10425143890129293 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 4896 0.10301009987098489 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 4845 0.10193707799732882 No Hit
TTAGCTTTGAAGTGGATCCTACCAGTTTTATCATCTTTTCACATAAAAGT 4828 0.10157940403944345 No Hit
TGAGACCGCACCAGCGACACA 4790 0.10077989754534675 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2784775 3.506057 6.646789 2
TTTTT 2511425 3.4093912 4.547809 8
GGGGG 697145 3.2258253 8.836767 4
GGAGG 779605 2.7805748 5.1961446 4
CCTCC 791060 2.650535 9.379711 6
CCCCA 783755 2.5867763 6.0747533 1
CCAGC 758090 2.551054 7.770717 5
CCTGG 722260 2.5156972 5.204654 9
CAGCC 744150 2.5041444 5.646821 6
TGGGG 669765 2.42509 11.083379 3
CTCCC 688015 2.3052711 5.340551 3
GCTGG 607930 2.1589308 5.5222816 7
GAGGG 603690 2.1531482 7.108233 9
TTTTC 1263500 2.1499274 5.1114407 9
GGGGA 596275 2.1267014 5.6863275 7
CTCCA 770370 2.0285516 5.01118 7
CTCCT 756530 2.0223596 5.172678 4
AAGAA 1215480 1.9853379 5.0302143 2
GCCAG 561230 1.9255736 6.9728727 4
TCCAG 713570 1.915771 5.479799 7
CCCAC 576650 1.9032282 6.154715 9
TGGCT 680140 1.8900411 6.03747 3
TGCAG 683005 1.869611 5.7973256 3
TCCTC 694770 1.8572626 5.900343 5
TGTTT 1060415 1.8396902 5.5759053 3
GGCTC 521320 1.815805 7.213619 1
CACCA 697960 1.810388 5.1352158 8
GCCCA 534960 1.8001977 5.729063 4
AGGGG 489000 1.7440897 7.344864 6
AAACA 1087865 1.7427747 5.687862 2
GGCCC 374185 1.6335899 5.6841636 1
GAAAG 763930 1.6188177 5.355327 5
GCTCC 456790 1.5604901 7.8134546 2
TGCCC 454710 1.5533844 10.982909 3
TAAAA 1174605 1.5012956 8.605615 1
ATGGA 691540 1.4876722 10.144277 2
GATGG 528365 1.4746255 5.2516804 1
CATGG 536115 1.4675244 41.407623 1
GGGGT 374295 1.3552504 5.0937963 4
GCAGT 493110 1.3498054 5.748832 4
TGCTC 476855 1.2996874 5.9313965 1
TGGGT 443975 1.2579163 6.0867424 3
ATGTG 574675 1.2550406 7.314814 2
ATGAA 750990 1.2452781 8.615405 2
AGACA 597875 1.2426089 5.197672 2
ATGGG 440550 1.2295407 14.750002 2
TGAGA 570685 1.2276834 5.133166 1
ATGGC 436275 1.1942292 9.859522 2
ATGCC 442735 1.1886414 11.077301 2
CATGT 497935 1.0665662 31.072886 1
TAAAG 623645 1.0341169 6.7830486 1
CATGA 489505 1.032825 28.926262 1
ATGTT 604285 1.0326785 7.8763375 2
ATGCT 468690 1.003924 7.513846 2
ATGGT 456545 0.99705493 7.851887 2
AGACT 463935 0.9788739 5.031641 1
ATGAC 456875 0.96397775 6.8983517 2
ATGAT 570960 0.9611329 6.888852 2
ATGTA 538740 0.9068949 8.703738 2
CATGC 336750 0.9040961 31.206514 1
TAAGA 486950 0.8074517 5.0442624 1
ATGCA 356645 0.7524987 8.885093 2
TAACT 451620 0.7456423 5.6052933 1
TAAGT 441970 0.7439959 6.4453473 1
TAAAC 435595 0.70842636 6.0734377 1
TAAGG 301690 0.6490092 5.7355886 1
TGCCG 158640 0.5525575 7.6032033 5
GCCGT 123235 0.4292387 5.043119 6
Produced by FastQC (version 0.10.0)