FastQCFastQC Report
Wed 20 Mar 2013
8016-8-39.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-39.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4662248
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 33984 0.7289187533567498 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 11145 0.2390477726624581 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11046 0.23692433349748876 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11029 0.23655970252976677 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10857 0.2328704950916382 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10262 0.22010841122136787 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10175 0.21824235862184937 No Hit
TGAGACCGCACCAGCGACACA 9949 0.21339491163919208 No Hit
TGTGTCGCTGGTGCGGTCTCA 9836 0.21097118814786342 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 9535 0.20451507513113845 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 8243 0.1768031215842658 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 8220 0.17630979733381835 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7791 0.1671082276189512 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 7549 0.16191759854902613 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 7537 0.1616602119835753 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7419 0.15912924408997547 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 7402 0.15876461312225346 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7342 0.1574776802949993 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 7333 0.1572846403709112 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7120 0.15271602883415897 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7015 0.1504638963864642 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 6853 0.146989177752878 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 6802 0.14589528484971198 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6707 0.14385764120655958 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6691 0.1435144591192918 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 6665 0.14295678822748167 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 6643 0.1424849128574885 No Hit
GAGACCGCACCAGCGACACC 6558 0.14066175801887842 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6432 0.13795919908164475 No Hit
GGTGTCGCTGGTGCGGTCTC 6413 0.13755167035301427 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 6333 0.1358357599166754 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 6278 0.13465607149169243 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6241 0.1338624629148857 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6233 0.1336908718712518 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6081 0.13043064204220797 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6032 0.1293796468999504 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6001 0.1287147316058691 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 5960 0.12783532750724544 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 5948 0.12757794094179462 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 5927 0.12712751445225565 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5907 0.12669853684317095 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5657 0.12133631672961197 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5654 0.12127197008824928 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 5597 0.12004938390235782 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5388 0.11556656788742255 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 5311 0.1139150040924464 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 5266 0.11294980447200578 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5149 0.11044028545886019 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5123 0.10988261456705006 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5121 0.10983971680614157 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 5114 0.10968957464296193 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 5104 0.10947508583841958 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 5076 0.10887451718570096 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 5061 0.10855278397888744 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 5047 0.10825249965252814 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 5023 0.10773772652162647 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5016 0.10758758435844684 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 5016 0.10758758435844684 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 5014 0.10754468659753835 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 4992 0.10707281122754517 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 4989 0.10700846458618245 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 4984 0.10690122018391128 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 4967 0.10653658921618928 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 4965 0.10649369145528081 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 4958 0.10634354929210114 No Hit
GGCTGTGAGTTGGGAGGCGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 4957 0.10632210041164691 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 4884 0.10475633213848771 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 4851 0.10404851908349792 No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA 4848 0.1039841724421352 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 4831 0.1036195414744132 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 4778 0.10248275081033871 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 4732 0.10149610230944385 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT 4722 0.1012816135049015 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 4722 0.1012816135049015 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2924700 3.7907655 7.371223 60-64
GGGGG 802675 3.7309206 8.565836 40-44
TTTTT 2386930 3.455256 4.1866555 60-64
GGAGG 754055 2.7148957 5.474136 4
CCAGC 756885 2.629718 8.698866 5
CCTCC 737290 2.5879722 9.384997 6
CCTGG 715705 2.5725822 5.9246826 9
CCCCA 744190 2.5550897 5.523578 1
CAGCC 726250 2.52328 6.4060493 6
TGGGG 648170 2.385822 9.715099 3
CTCCC 660370 2.3179743 5.2454057 3
GCTGG 607890 2.211145 9.366283 7
GGGGA 612925 2.2067723 5.4054255 7
GGCAG 611925 2.1771653 5.252581 8
GAGGG 594005 2.1386526 7.170717 9
CTCCT 710605 1.998846 5.8761687 4
CTCCA 710675 1.9553441 5.4975343 6
TCCAG 692800 1.9289323 6.3075957 7
GCCAG 545105 1.9165336 7.4604683 4
CCCAC 553110 1.8990388 6.449887 9
TGGCT 658720 1.8974305 7.119446 3
CACCA 699040 1.8812873 6.443552 8
CCCTG 525335 1.8660129 5.0034513 1
TGGTG 638375 1.8607922 6.977863 9
TGTTT 1016710 1.8585095 5.8588667 3
TCCTC 659345 1.8546581 6.681564 5
AGGGG 508930 1.832349 6.8736024 6
GGCTC 504340 1.8128366 7.6305313 4
GCCCA 516660 1.7950814 6.275963 4
GGCCC 370940 1.6638305 5.2814517 1
CTGGT 536660 1.5458391 6.099792 8
TGCCC 434760 1.5442864 11.603673 3
GCTCC 433795 1.5408587 7.2010393 2
TAAAA 1148810 1.5222739 8.898352 1
ATGGA 664450 1.467436 9.659553 2
GATGG 513165 1.4631203 6.017038 1
CATGG 508470 1.4326212 39.52242 1
GCAGT 481475 1.3565624 5.072433 4
TGCTC 455580 1.2967992 5.930818 1
ATGTG 561430 1.267627 7.2316055 2
TGGGT 431255 1.2570604 5.7835803 3
TGAGA 558010 1.2323635 6.1370907 1
ATGAA 718415 1.2289838 8.730321 2
ATGGC 435940 1.228267 10.828163 2
ATGCC 434065 1.208548 11.846767 2
AGACA 564125 1.204249 5.2293954 2
ATGGG 421430 1.2015684 13.888479 2
GTCTT 502705 1.1467034 5.1297264 9
GAGAC 406610 1.1205858 6.2653704 2
CATGT 486230 1.0848774 31.926151 1
CATGA 479735 1.0469872 28.931934 1
GTGTC 361590 1.0415531 6.287349 2
ATGTT 581510 1.0397427 7.8476963 2
TAAAG 598365 1.0236156 6.877161 1
ATGCT 451375 1.0071088 7.750108 2
ATGGT 430255 0.97145295 7.153262 2
AGACC 354905 0.96654534 6.067401 3
ATGAC 441490 0.9635202 7.248505 2
ATGAT 549890 0.96171314 6.741609 2
CACTC 347995 0.95746994 5.0394783 1
GCGCC 209765 0.9408891 5.291729 8
ATGTA 528380 0.9240939 9.380967 2
CATGC 329265 0.91675794 31.536383 1
CGCTG 234330 0.84229285 7.5901666 6
TAACT 437645 0.7563707 5.7182446 1
TAAGT 425135 0.74352676 6.574044 1
ATGCA 337635 0.73686415 8.314729 2
TAAAC 408735 0.69096434 5.377548 1
TGCCG 157905 0.56758523 8.329912 5
GACCG 141210 0.49647987 5.0475063 4
GCCGT 129710 0.46623912 5.5364747 6
CCGTC 128870 0.45775184 5.4562783 7
TCGCT 154420 0.43955338 5.4858685 5
CGTCT 154350 0.43935415 5.0556927 8
ACCGC 122205 0.4245885 5.105331 5
GTCGC 103765 0.3729805 6.9076214 4
TGTCG 121425 0.34976244 5.624549 3
Produced by FastQC (version 0.10.0)