##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-40.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5925140	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.8771500757788	24.0	24.0	31.0	23.0	31.0
2	30.96578730155073	31.0	31.0	32.0	27.0	33.0
3	32.579140414235866	33.0	33.0	34.0	29.0	35.0
4	33.778736631700426	34.0	33.0	36.0	29.0	37.0
5	35.33084496209657	36.0	35.0	37.0	33.0	37.0
6	35.67021247459312	36.0	36.0	38.0	33.0	38.0
7	35.681030864413444	37.0	35.0	37.0	32.0	37.0
8	35.53285546148401	36.0	36.0	37.0	32.0	38.0
9	35.54758644387951	37.0	36.0	37.0	31.0	38.0
10-14	35.90583000204936	37.0	36.0	37.4	32.2	38.0
15-19	36.88569113911945	38.0	37.2	38.4	32.6	39.4
20-24	36.51906637365254	37.4	36.0	38.8	32.8	39.8
25-29	36.17040154860033	37.6	36.2	38.0	32.6	38.2
30-34	35.988555944227095	37.6	35.6	38.2	32.2	38.6
35-39	35.99857518091763	37.8	36.4	38.0	31.6	38.6
40-44	35.95295806262124	37.4	35.8	38.4	31.6	38.8
45-49	35.90552258554654	37.6	35.8	38.2	31.6	38.6
50-54	36.109697471054915	37.6	36.2	38.2	33.0	39.0
55-59	35.82730491064882	37.4	35.6	38.2	31.8	38.6
60-64	35.86506154155802	37.4	35.6	38.2	32.4	38.6
65-69	35.6659068289101	37.4	35.8	38.2	31.4	38.6
70-74	35.69435566300617	37.2	36.0	38.4	31.6	39.0
75-79	35.394246997427146	37.4	35.4	37.6	31.0	38.4
80-84	35.505337406994435	37.0	35.8	38.2	31.0	38.4
85-89	35.204660200446455	37.2	35.4	37.6	30.4	38.2
90-94	35.22220376200064	37.0	35.0	38.2	30.8	38.4
95-99	35.04739023232064	37.4	35.0	37.6	29.8	38.2
100	33.958386582562724	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	4.0
1	8.0
2	55.0
3	194.0
4	0.0
5	19.0
6	65.0
7	216.0
8	360.0
9	21898.0
10	904.0
11	1311.0
12	2101.0
13	3781.0
14	4988.0
15	5935.0
16	6925.0
17	8088.0
18	9560.0
19	11404.0
20	12429.0
21	12254.0
22	12505.0
23	14585.0
24	18153.0
25	23790.0
26	32008.0
27	43413.0
28	58240.0
29	76653.0
30	103971.0
31	135271.0
32	165815.0
33	217301.0
34	316296.0
35	561204.0
36	1667748.0
37	2375678.0
38	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.37955228618217	18.994459799525107	21.47210266462103	39.15388524967169
2	18.071334415441388	51.57270512329594	15.004523844217104	15.351436617045575
3	19.703045828926175	34.015100191785585	29.955983191733733	16.325870787554507
4	33.76292347323832	24.635850818348196	21.023686225155775	20.577539483257716
5	20.620407066705557	27.180789378087706	26.326047144895693	25.872756410311048
6	20.36810796811228	29.16686138161511	25.834591063516786	24.630439586755823
7	21.909398302802025	27.95802150053864	27.509074863703443	22.623505332955894
8	21.300277076802864	27.63472021484383	28.809625996899495	22.255376711453806
9	21.815598546484114	28.42118421215077	26.825056825118555	22.938160416246564
10-14	22.048839542161446	27.423521241479833	26.779985424615255	23.747653791743474
15-19	21.57418015145334	27.214391401790728	27.200813649709367	24.010614797046568
20-24	21.888057557444647	26.699925433591133	27.43004681955122	23.981970189413
25-29	21.773685866083696	27.154486992188296	28.13480915929343	22.93701798243458
30-34	22.47052421511456	27.433232379211425	27.467457785929273	22.628785619744743
35-39	22.43267907563066	26.381645175953555	28.53305732232887	22.652618426086914
40-44	22.614368958559265	27.285284063984367	27.426501215300696	22.673845762155672
45-49	22.251642756284838	27.79956145547352	27.85036172000521	22.09843406823643
50-54	22.640641653506396	27.43644784028495	27.53007502876306	22.392835477445594
55-59	21.94911560526235	28.294321935991494	27.39093860040187	22.36562385834429
60-64	22.461606584570475	27.8162888320453	28.04547946790802	21.67662511547621
65-69	22.454467181278805	28.691319344725123	27.697553067509666	21.156660406486406
70-74	22.190084201526673	28.34160125012965	27.258793770439382	22.20952077790429
75-79	22.777930260161643	28.2443537718984	27.43459995179257	21.54311601614739
80-84	21.895071713902446	28.20794695953405	27.665084447683036	22.23189687888047
85-89	22.213145725802402	27.954355783864727	27.824756555225783	22.007741935107088
90-94	22.320741954491286	27.69556169295435	27.777517304942844	22.206179047611517
95-99	22.465254901310285	26.95615688724911	28.10814595477737	22.470442256663233
100	23.638355765782098	26.867698454942396	26.538378395404184	22.955567383871323
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	59.533437535853864
2	33.42277103248696
3	36.028916616480686
4	54.340462956496026
5	46.4931634770166
6	44.998547554868104
7	44.53290363575792
8	43.55565378825667
9	44.75375896273068
10-14	45.79649333390492
15-19	45.584794948499905
20-24	45.870027746857645
25-29	44.71070384851827
30-34	45.099309834859305
35-39	45.08529750171758
40-44	45.28821472071493
45-49	44.35007682452127
50-54	45.033477130951994
55-59	44.314739463606635
60-64	44.13823170004668
65-69	43.61112758776521
70-74	44.39960497943097
75-79	44.321046276309026
80-84	44.126968592782916
85-89	44.22088766090949
90-94	44.5269210021028
95-99	44.93569715797352
100	46.59392314965342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22243.0
1	22243.5
2	22247.5
3	22250.5
4	22252.0
5	22272.0
6	22291.5
7	22317.0
8	22334.0
9	22341.0
10	22364.5
11	22389.0
12	22413.0
13	22493.0
14	22539.0
15	22984.5
16	23216.0
17	23119.0
18	23637.5
19	24540.0
20	25854.5
21	27675.5
22	32136.5
23	39723.0
24	55472.0
25	68910.0
26	81265.0
27	98798.5
28	104005.0
29	114844.0
30	141153.0
31	159814.5
32	164752.0
33	180630.5
34	201654.0
35	201174.5
36	193366.0
37	181422.0
38	202585.5
39	225683.0
40	232743.5
41	248229.0
42	236977.5
43	239012.5
44	249706.0
45	233367.0
46	211182.0
47	187803.0
48	180390.5
49	191861.0
50	194300.0
51	195389.0
52	167343.5
53	113735.5
54	97168.0
55	96892.0
56	79086.0
57	66680.0
58	88424.0
59	90010.0
60	85954.0
61	102472.5
62	93216.0
63	90400.5
64	91264.0
65	85018.0
66	88542.0
67	82416.5
68	63674.5
69	57908.0
70	57579.0
71	54191.5
72	59525.5
73	47724.0
74	31206.0
75	36238.5
76	39867.0
77	38897.0
78	36765.5
79	35110.0
80	32939.0
81	31050.0
82	29577.5
83	30698.5
84	31397.0
85	29332.5
86	31402.0
87	31121.0
88	33354.0
89	37973.5
90	43261.5
91	48679.5
92	45897.0
93	43091.0
94	43060.5
95	43068.5
96	43064.0
97	43056.5
98	43055.0
99	43056.5
100	43056.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05302828287601643
2	0.00772551908372633
3	0.0022875474221289204
4	0.007593987460428206
5	0.00468764276002951
6	8.387650502694148E-4
7	4.972268458603635E-4
8	1.8861557539410367E-4
9	1.0288182273667708E-4
10-14	0.0014952406421770484
15-19	0.018521041343491647
20-24	0.016996296239556546
25-29	0.008853867996042441
30-34	0.008407049067035245
35-39	0.005745671003570168
40-44	0.01325801965628662
45-49	0.012314539550109516
50-54	0.010963611318232475
55-59	0.011440223953599797
60-64	0.05465785590854147
65-69	0.044012404232498956
70-74	0.027616162340212146
75-79	0.07446991434260614
80-84	0.0650607190386563
85-89	0.015247648749061439
90-94	0.007429328875052852
95-99	0.009755423210065879
100	0.008923561633409214
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	22624.0
2-3	3112.0
4-5	57482.0
6-7	9954.0
8-9	58.0
10-11	45.0
12-13	99.0
14-15	214.0
16-17	301.0
18-19	1994.0
20-21	27046.0
22-23	3356.0
24-25	3641.0
26-27	2442.0
28-29	1862.0
30-31	3163.0
32-33	2527.0
34-35	2498.0
36-37	2025.0
38-39	1913.0
40-41	3105.0
42-43	1737.0
44-45	2994.0
46-47	5059.0
48-49	1805.0
50-51	20270.0
52-53	2438.0
54-55	23998.0
56-57	14781.0
58-59	46439.0
60-61	2487.0
62-63	16836.0
64-65	5997.0
66-67	8093.0
68-69	9946.0
70-71	27472.0
72-73	27401.0
74-75	18033.0
76-77	18599.0
78-79	5714.0
80-81	12300.0
82-83	11834.0
84-85	8663.0
86-87	3796.0
88-89	5291.0
90-91	11825.0
92-93	3832.0
94-95	3889.0
96-97	119957.0
98-99	0.0
100-101	5334193.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	96.93946056487115
#Duplication Level	Relative count
1	100.0
2	6.360092670907954
3	1.8914181345689454
4	1.0088380588141557
5	0.7612253153385062
6	0.6502899030387722
7	0.6374189436006815
8	0.6306770124664436
9	0.6104512190637297
10++	10.030154819254955
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	21309	0.35963707186665633	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	14099	0.23795218340832455	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	13977	0.23589316033038882	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	12265	0.20699932828591394	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	12262	0.20694869657088272	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	12247	0.20669553799572668	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	12176	0.20549725407332148	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	11817	0.19943832550792048	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	11751	0.19832442777723397	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	10956	0.18490702329396436	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	10812	0.1824767009724665	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	9299	0.1569414393583949	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	9185	0.1550174341872091	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	9155	0.15451111703689702	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	9072	0.153110306254367	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	9031	0.15241833948227385	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	9030	0.1524014622439301	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	8801	0.1485365746632147	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	8729	0.14732141350246578	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	8681	0.14651130606196647	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	8659	0.14614000681840428	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	8501	0.1434734031600941	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	8492	0.1433215080150005	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	8323	0.14046925473490923	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	8288	0.13987855139287847	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	8249	0.1392203390974728	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	8239	0.13905156671403543	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	8203	0.138443986133661	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	8138	0.13734696564131818	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	8134	0.13727945668794322	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	7645	0.12902648713785664	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	7607	0.12838515208079473	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	7569	0.12774381702373278	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	7553	0.127473781210233	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	7545	0.12733876330348312	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	7485	0.126326129002859	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	7467	0.12602233871267177	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	7200	0.12151611607489443	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	7141	0.12052035901261404	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	7094	0.11972712881045848	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	7003	0.11819130012117858	No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT	6856	0.11571034608464947	No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA	6810	0.11493399312083766	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	6588	0.11118724620852839	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	6474	0.10926324103734258	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	6426	0.10845313359684329	No Hit
TGAGACCGCACCAGCGACACA	6391	0.10786243025481254	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	6380	0.10767678063303146	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	6329	0.10681604147750096	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	6327	0.10678228700081349	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	6325	0.10674853252412601	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	6295	0.10624221537381395	No Hit
TGTGTCGCTGGTGCGGTCTCA	6293	0.10620846089712647	No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG	6258	0.10561775755509575	No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA	6196	0.10457136877778415	No Hit
CCCC	6178	0.10426757848759692	No Hit
GGGG	6167	0.10408192886581583	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	6128	0.10342371657041013	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	6062	0.10230981883972362	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	6039	0.1019216423578177	No Hit
GGCCC	6037	0.10188788788113023	No Hit
GGGCC	6036	0.10187101064278649	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	5982	0.10095963977222479	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3325640	3.6654234	6.6810956	2
TTTTT	2874295	3.4412866	4.306131	95-96
GGGGG	958230	3.2379367	8.399323	4
GGAGG	984905	2.6600082	5.391307	4
CCAGC	1021055	2.6295824	7.640216	5
CCTCC	1005405	2.5910935	9.532575	6
CCCCA	1021910	2.5903986	5.437085	1
CCTGG	966000	2.5697246	5.045489	9
CAGCC	994115	2.560202	5.5603795	6
TGGGG	876785	2.40752	9.464078	3
CTCCC	895135	2.3069096	5.5113482	3
GCTGG	805360	2.1766224	5.791098	7
GGGGA	782980	2.1146538	5.370797	7
GAGGG	775585	2.0946817	6.3788505	9
CTCCT	951970	2.0254762	5.9604797	4
CTCCA	949820	1.9877285	6.058713	6
CCCTC	769350	1.982741	5.0192127	5
TCCAG	915620	1.9467704	6.510028	7
CCCAC	762385	1.9325391	5.8366237	9
GCCAG	733290	1.9186558	6.9376297	4
TGGCT	865570	1.9009621	7.4188213	3
TGTTT	1278600	1.883849	5.773914	3
TGCAG	867795	1.8745642	5.3085904	3
TCCTC	872300	1.8559649	6.8765	5
GGCTC	690970	1.8380977	7.9279046	1
CACCA	888875	1.8296515	5.0835004	8
GCCCA	701215	1.8058797	6.450841	4
AGGGG	646495	1.7460384	6.835192	6
GGCCC	525250	1.6924411	6.995127	1
GCTCC	606160	1.5871319	8.38516	2
TAAAA	1400100	1.5689032	8.747345	1
TGCCC	588920	1.5419917	12.150174	3
CTGGT	700800	1.5390948	5.0412445	6
GATGG	669375	1.4690491	5.9288287	1
ATGGA	831615	1.4587443	10.373713	2
CATGG	654700	1.4142478	41.03583	1
TGCTC	603995	1.3056325	5.973253	1
TGGGT	566545	1.2641231	5.89816	3
ATGTG	708190	1.2629752	8.047964	2
ATGAA	900220	1.2621055	9.156226	2
ATGGC	568180	1.2273518	11.3608055	2
TGAGA	697450	1.2234043	5.369437	1
AGACA	716605	1.2169282	5.2135115	2
ATGCC	570810	1.2136432	12.446205	2
ATGGG	549955	1.206963	14.366744	2
GTCTT	625040	1.1154684	5.1010747	9
GAGAC	515525	1.0953295	5.3300676	2
CATGT	607530	1.066422	31.95869	1
ATGTT	731805	1.0605193	7.930466	2
CATGA	612875	1.0581448	28.906685	1
TAAAG	733790	1.0287712	6.8220034	1
ATGCT	568595	0.9980777	7.6553955	2
ATGAT	683695	0.9745351	6.7541337	2
ATGGT	544105	0.97034836	7.5739665	2
ATGAC	554920	0.958084	7.0434756	2
ATGTA	648515	0.9243897	8.970729	2
CATGC	429965	0.9141818	31.806887	1
TAACT	538115	0.75496453	5.4515224	1
TAAGT	522895	0.74533165	6.124663	1
ATGCA	429920	0.7422682	8.929732	2
TAAAC	503280	0.69450116	5.2043285	1
TGCCG	218325	0.58078164	8.468305	5
CCGTC	185890	0.48672292	5.226425	7
GCCGT	170515	0.45359895	5.286302	6
>>END_MODULE