FastQCFastQC Report
Wed 20 Mar 2013
8016-8-40.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-40.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5925140
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 21309 0.35963707186665633 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 14099 0.23795218340832455 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 13977 0.23589316033038882 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 12265 0.20699932828591394 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 12262 0.20694869657088272 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 12247 0.20669553799572668 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 12176 0.20549725407332148 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 11817 0.19943832550792048 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 11751 0.19832442777723397 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 10956 0.18490702329396436 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 10812 0.1824767009724665 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 9299 0.1569414393583949 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 9185 0.1550174341872091 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 9155 0.15451111703689702 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 9072 0.153110306254367 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 9031 0.15241833948227385 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 9030 0.1524014622439301 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 8801 0.1485365746632147 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 8729 0.14732141350246578 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 8681 0.14651130606196647 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 8659 0.14614000681840428 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 8501 0.1434734031600941 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 8492 0.1433215080150005 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 8323 0.14046925473490923 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 8288 0.13987855139287847 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 8249 0.1392203390974728 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 8239 0.13905156671403543 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 8203 0.138443986133661 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 8138 0.13734696564131818 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 8134 0.13727945668794322 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 7645 0.12902648713785664 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 7607 0.12838515208079473 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7569 0.12774381702373278 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7553 0.127473781210233 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7545 0.12733876330348312 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 7485 0.126326129002859 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 7467 0.12602233871267177 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 7200 0.12151611607489443 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 7141 0.12052035901261404 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 7094 0.11972712881045848 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 7003 0.11819130012117858 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 6856 0.11571034608464947 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 6810 0.11493399312083766 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6588 0.11118724620852839 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 6474 0.10926324103734258 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6426 0.10845313359684329 No Hit
TGAGACCGCACCAGCGACACA 6391 0.10786243025481254 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 6380 0.10767678063303146 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6329 0.10681604147750096 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 6327 0.10678228700081349 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6325 0.10674853252412601 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 6295 0.10624221537381395 No Hit
TGTGTCGCTGGTGCGGTCTCA 6293 0.10620846089712647 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 6258 0.10561775755509575 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 6196 0.10457136877778415 No Hit
CCCC 6178 0.10426757848759692 No Hit
GGGG 6167 0.10408192886581583 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 6128 0.10342371657041013 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 6062 0.10230981883972362 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 6039 0.1019216423578177 No Hit
GGCCC 6037 0.10188788788113023 No Hit
GGGCC 6036 0.10187101064278649 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 5982 0.10095963977222479 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3325640 3.6654234 6.6810956 2
TTTTT 2874295 3.4412866 4.306131 95-96
GGGGG 958230 3.2379367 8.399323 4
GGAGG 984905 2.6600082 5.391307 4
CCAGC 1021055 2.6295824 7.640216 5
CCTCC 1005405 2.5910935 9.532575 6
CCCCA 1021910 2.5903986 5.437085 1
CCTGG 966000 2.5697246 5.045489 9
CAGCC 994115 2.560202 5.5603795 6
TGGGG 876785 2.40752 9.464078 3
CTCCC 895135 2.3069096 5.5113482 3
GCTGG 805360 2.1766224 5.791098 7
GGGGA 782980 2.1146538 5.370797 7
GAGGG 775585 2.0946817 6.3788505 9
CTCCT 951970 2.0254762 5.9604797 4
CTCCA 949820 1.9877285 6.058713 6
CCCTC 769350 1.982741 5.0192127 5
TCCAG 915620 1.9467704 6.510028 7
CCCAC 762385 1.9325391 5.8366237 9
GCCAG 733290 1.9186558 6.9376297 4
TGGCT 865570 1.9009621 7.4188213 3
TGTTT 1278600 1.883849 5.773914 3
TGCAG 867795 1.8745642 5.3085904 3
TCCTC 872300 1.8559649 6.8765 5
GGCTC 690970 1.8380977 7.9279046 1
CACCA 888875 1.8296515 5.0835004 8
GCCCA 701215 1.8058797 6.450841 4
AGGGG 646495 1.7460384 6.835192 6
GGCCC 525250 1.6924411 6.995127 1
GCTCC 606160 1.5871319 8.38516 2
TAAAA 1400100 1.5689032 8.747345 1
TGCCC 588920 1.5419917 12.150174 3
CTGGT 700800 1.5390948 5.0412445 6
GATGG 669375 1.4690491 5.9288287 1
ATGGA 831615 1.4587443 10.373713 2
CATGG 654700 1.4142478 41.03583 1
TGCTC 603995 1.3056325 5.973253 1
TGGGT 566545 1.2641231 5.89816 3
ATGTG 708190 1.2629752 8.047964 2
ATGAA 900220 1.2621055 9.156226 2
ATGGC 568180 1.2273518 11.3608055 2
TGAGA 697450 1.2234043 5.369437 1
AGACA 716605 1.2169282 5.2135115 2
ATGCC 570810 1.2136432 12.446205 2
ATGGG 549955 1.206963 14.366744 2
GTCTT 625040 1.1154684 5.1010747 9
GAGAC 515525 1.0953295 5.3300676 2
CATGT 607530 1.066422 31.95869 1
ATGTT 731805 1.0605193 7.930466 2
CATGA 612875 1.0581448 28.906685 1
TAAAG 733790 1.0287712 6.8220034 1
ATGCT 568595 0.9980777 7.6553955 2
ATGAT 683695 0.9745351 6.7541337 2
ATGGT 544105 0.97034836 7.5739665 2
ATGAC 554920 0.958084 7.0434756 2
ATGTA 648515 0.9243897 8.970729 2
CATGC 429965 0.9141818 31.806887 1
TAACT 538115 0.75496453 5.4515224 1
TAAGT 522895 0.74533165 6.124663 1
ATGCA 429920 0.7422682 8.929732 2
TAAAC 503280 0.69450116 5.2043285 1
TGCCG 218325 0.58078164 8.468305 5
CCGTC 185890 0.48672292 5.226425 7
GCCGT 170515 0.45359895 5.286302 6
Produced by FastQC (version 0.10.0)