##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-41.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4201668	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.8786301059484	24.0	24.0	31.0	23.0	31.0
2	30.971687050798195	31.0	31.0	32.0	27.0	33.0
3	32.58251654160122	33.0	33.0	34.0	29.0	35.0
4	33.78441590853294	34.0	33.0	36.0	29.0	37.0
5	35.32772583613134	36.0	35.0	37.0	33.0	37.0
6	35.66612253867665	36.0	36.0	38.0	33.0	38.0
7	35.67707195228995	37.0	35.0	37.0	32.0	37.0
8	35.53009710274265	36.0	36.0	37.0	32.0	38.0
9	35.53683960402497	37.0	36.0	37.0	31.0	38.0
10-14	35.89768814731277	37.0	36.0	37.4	32.2	38.0
15-19	36.87789093739038	38.0	37.2	38.4	32.6	39.4
20-24	36.51768339143405	37.4	36.0	38.8	32.8	39.8
25-29	36.169809098417566	37.6	36.2	38.0	32.6	38.2
30-34	35.98633225795931	37.6	35.6	38.2	32.2	38.6
35-39	35.996720401032405	37.8	36.4	38.0	31.6	38.6
40-44	35.94634780610047	37.4	35.8	38.4	31.6	38.8
45-49	35.899517277054265	37.6	35.8	38.2	31.6	38.6
50-54	36.1041622673671	37.6	36.2	38.2	33.0	39.0
55-59	35.82463099854461	37.4	35.6	38.2	31.4	38.6
60-64	35.86551374864582	37.6	35.6	38.2	32.4	38.6
65-69	35.66581035199293	37.4	35.8	38.2	31.4	38.6
70-74	35.690061009614254	37.2	36.0	38.4	31.6	39.0
75-79	35.393895068456885	37.4	35.4	37.6	31.0	38.4
80-84	35.502358895717485	37.0	35.8	38.2	31.0	38.4
85-89	35.20077032403874	37.2	35.4	37.6	30.6	38.2
90-94	35.21958388172328	37.0	35.0	38.2	30.8	38.4
95-99	35.04257407553105	37.4	35.0	37.6	29.6	38.2
100	33.974752196753	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	13.0
2	40.0
3	189.0
4	0.0
5	17.0
6	44.0
7	171.0
8	249.0
9	17121.0
10	666.0
11	996.0
12	1593.0
13	2804.0
14	3525.0
15	4333.0
16	5281.0
17	6277.0
18	7141.0
19	8323.0
20	9371.0
21	8834.0
22	8881.0
23	9799.0
24	12062.0
25	15835.0
26	20856.0
27	28616.0
28	38931.0
29	51429.0
30	67665.0
31	88002.0
32	113889.0
33	151952.0
34	224077.0
35	401772.0
36	1184807.0
37	1706103.0
38	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.45546776735761	19.467849584348617	21.67549408947512	39.40118855881865
2	17.573710129504562	52.65501321626339	14.831998799086502	14.939277855145546
3	19.017876095025557	34.6431808663825	30.577889519307444	15.761053519284498
4	33.710295332322076	25.12322148549516	21.042048427950366	20.124434754232396
5	20.413571811133657	27.40124948002121	26.746823733948833	25.4383549748963
6	20.236799682856287	29.394922923639893	25.983856503959746	24.384420889544074
7	22.00643850655611	28.027715260440157	27.25971704041605	22.706129192587685
8	21.16908137269594	27.73059193765922	28.916673160497524	22.18365352914731
9	21.70956295349093	28.44311427497918	27.11331779426423	22.73400497726566
10-14	21.945711827713183	27.55140881082067	26.878366070930586	23.62451329053556
15-19	21.46897317219093	27.383445357538694	27.34160268932932	23.80597878094106
20-24	21.676605065673918	26.974234470171922	27.617685129400883	23.731475334753284
25-29	21.65632729468295	27.384422021487016	28.232117512809285	22.727133171020757
30-34	22.37199403146596	27.47618504316699	27.61768714749885	22.53413377786821
35-39	22.23497402587448	26.480941392619854	28.803564084111905	22.48052049739376
40-44	22.48488399528193	27.529924332311502	27.405158465618307	22.580033206788265
45-49	22.048500008847668	27.975194245242243	27.97453310068823	22.00177264522186
50-54	22.53002648344742	27.572666077603646	27.67372217183292	22.223585267116018
55-59	21.853852460930824	28.3298762251181	27.512472756372823	22.303798557578247
60-64	22.340162401245543	27.99027091694699	28.143508150433348	21.526058531374115
65-69	22.266673144642805	28.90635068523822	27.7008728939691	21.126103276149884
70-74	22.000975506868095	28.43857617456803	27.421262588053736	22.13918573051014
75-79	22.672895530126212	28.262539953944056	27.6613067951871	21.403257720742634
80-84	21.78410503005171	28.27563946292232	27.75329313076891	22.186962376257064
85-89	22.17363196795512	27.99270685617568	28.03550376460755	21.79815741126165
90-94	22.14699437088522	27.717929336433784	27.915909688523417	22.219166604157582
95-99	22.23385742141156	27.074458958891878	28.286827013386333	22.40485660631023
100	23.68640214350031	26.947796818611515	26.74306654829941	22.622734489588762
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	58.85665632617626
2	32.5129879846501
3	34.77892961431006
4	53.83473008655447
5	45.851926786029956
6	44.621220572400354
7	44.7125676991438
8	43.35273490184325
9	44.443567930756586
10-14	45.57022511824874
15-19	45.27495195313199
20-24	45.4080804004272
25-29	44.383460465703706
30-34	44.906127809334166
35-39	44.71549452326824
40-44	45.06491720207019
45-49	44.05027265406953
50-54	44.75361175056343
55-59	44.15765101850907
60-64	43.86622093261966
65-69	43.392776420792686
70-74	44.14016123737824
75-79	44.07615325086884
80-84	43.97106740630877
85-89	43.971789379216766
90-94	44.3661609750428
95-99	44.63871402772179
100	46.30913663308907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17609.0
1	17612.5
2	17619.5
3	17628.0
4	17636.0
5	17641.0
6	17648.5
7	17661.5
8	17674.5
9	17693.0
10	17699.5
11	17709.5
12	17735.0
13	17793.0
14	17834.0
15	18121.0
16	18315.5
17	18201.5
18	18408.0
19	19054.5
20	20081.0
21	21533.5
22	24440.5
23	30030.0
24	43849.0
25	55301.0
26	63681.5
27	75536.5
28	76591.0
29	85224.0
30	106936.0
31	118012.0
32	120974.5
33	136327.0
34	150432.0
35	145454.5
36	142000.5
37	133911.5
38	148848.0
39	164724.0
40	168883.0
41	182392.5
42	174710.5
43	174834.5
44	178970.0
45	168286.5
46	152623.0
47	133699.0
48	126420.0
49	136798.0
50	139950.0
51	136845.0
52	117454.0
53	78713.5
54	67589.0
55	67048.0
56	53860.0
57	46521.5
58	61329.0
59	61497.0
60	61515.0
61	79447.0
62	72578.0
63	66255.0
64	61535.5
65	54709.0
66	57488.0
67	51868.5
68	38907.0
69	34550.5
70	32236.0
71	27942.0
72	31466.5
73	23717.5
74	11558.5
75	14536.5
76	16983.0
77	16267.5
78	14433.5
79	13700.0
80	12434.5
81	10692.5
82	9905.5
83	10195.0
84	10019.0
85	8701.5
86	10403.5
87	10156.5
88	8246.5
89	8214.0
90	8625.0
91	9131.0
92	6989.5
93	4830.0
94	4786.5
95	4781.5
96	4775.5
97	4766.5
98	4782.5
99	4796.5
100	4796.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034010302575072565
2	0.007839744156641912
3	0.001888351571861458
4	0.006550026785784721
5	0.0046404233979715605
6	9.57550356376304E-4
7	4.3156556883578714E-4
8	2.1579452908119005E-4
9	7.193268251839198E-5
10-14	0.0014051199694307278
15-19	0.01678797900121214
20-24	0.01550568606147855
25-29	0.0082850807679161
30-34	0.007875417391063712
35-39	0.0054088611504594306
40-44	0.012193891913944242
45-49	0.01145204548309161
50-54	0.010466438846855953
55-59	0.009056418092245371
60-64	0.03855788348224991
65-69	0.03391298379599299
70-74	0.022098487015742945
75-79	0.050919643628564604
80-84	0.04625565080668353
85-89	0.011052420521181203
90-94	0.001360069272861631
95-99	0.008603976184193922
100	0.008266711037511853
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	17858.0
2-3	621.0
4-5	5863.0
6-7	6692.0
8-9	82.0
10-11	114.0
12-13	40.0
14-15	217.0
16-17	268.0
18-19	3076.0
20-21	30469.0
22-23	2751.0
24-25	2708.0
26-27	1292.0
28-29	1243.0
30-31	1727.0
32-33	1505.0
34-35	1587.0
36-37	1236.0
38-39	1361.0
40-41	3084.0
42-43	1656.0
44-45	1016.0
46-47	1192.0
48-49	1295.0
50-51	15267.0
52-53	1428.0
54-55	19292.0
56-57	13032.0
58-59	34723.0
60-61	1711.0
62-63	12256.0
64-65	5111.0
66-67	6595.0
68-69	7480.0
70-71	20763.0
72-73	19825.0
74-75	13346.0
76-77	14830.0
78-79	4619.0
80-81	10183.0
82-83	10292.0
84-85	6133.0
86-87	2285.0
88-89	4079.0
90-91	9502.0
92-93	3558.0
94-95	2895.0
96-97	87240.0
98-99	0.0
100-101	3786270.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	96.8188011628751
#Duplication Level	Relative count
1	100.0
2	5.684445209220203
3	1.8015456783103776
4	0.9658619064363362
5	0.715979740092621
6	0.6493943724142058
7	0.6404165700305994
8	0.5865497557289602
9	0.6404165700305994
10++	11.956188324368
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	16685	0.39710419766626015	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10410	0.2477587472403817	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10281	0.24468853798063056	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	9196	0.21886546009822766	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	9148	0.2177230566527389	No Hit
TGAGACCGCACCAGCGACACA	9010	0.2144386467469586	No Hit
TGTGTCGCTGGTGCGGTCTCA	8887	0.21151123791789359	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	8808	0.20963103224719326	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	8734	0.20786982693539804	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	8073	0.19213797948814615	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	7978	0.18987697266894957	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	7156	0.170313313664954	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	7087	0.1686711087120639	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	7065	0.1681475071328815	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	7050	0.16779050605616627	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	6820	0.16231648954653247	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	6741	0.16043628387583217	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	6707	0.1596270814352776	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	6694	0.1593176805021244	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	6671	0.15877027885116102	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	6615	0.1574374748314241	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	6599	0.15705667368292783	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	6589	0.15681867296511764	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	6544	0.15574766973497192	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	6481	0.15424826521276788	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	6392	0.15213005882425742	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	6271	0.14925025013875443	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	6246	0.148655248344229	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	6242	0.14856004805710493	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	6223	0.14810784669326563	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	6111	0.14544223865379177	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	6105	0.1452994382231057	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	6092	0.14499003728995247	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	6059	0.14420463492117894	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	5576	0.13270920025094796	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	5565	0.13244739946135678	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	5545	0.13197139802573643	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	5469	0.13016259257037918	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	5302	0.12618798058294944	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	5249	0.12492657677855556	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	5160	0.1228083703900451	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	5141	0.12235616902620577	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	5104	0.12147556637030818	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	5079	0.12088056457578275	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	5061	0.12045216328372448	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	4965	0.11816735639274688	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	4952	0.11785795545959366	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	4908	0.11681075230122893	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	4859	0.11564454878395913	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	4847	0.11535894792258693	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	4833	0.1150257469176527	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	4822	0.11476394612806153	No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC	4756	0.11319314139051444	No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG	4754	0.1131455412469524	No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG	4640	0.11043233306391653	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	4638	0.11038473292035449	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	4624	0.11005153191542026	No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA	4505	0.1072193233734793	No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG	4484	0.10671952186607796	No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA	4467	0.10631492064580067	No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT	4375	0.10412531404194714	No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA	4313	0.10264970959152414	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2453620	3.6971283	6.855226	2
TTTTT	2131655	3.49169	4.2778673	95-96
GGGGG	671515	3.2963011	8.631456	4
GGAGG	701600	2.7194304	5.4914117	4
CCAGC	724165	2.6674035	8.436196	5
CCTCC	703910	2.5920389	9.547411	6
CCCCA	706430	2.55824	5.424792	1
CCTGG	668765	2.5477457	5.728869	9
CAGCC	690670	2.544027	6.063372	6
TGGGG	608160	2.3969474	10.371142	3
CTCCC	630005	2.3198955	5.3298492	3
GCTGG	571530	2.2146273	8.475924	7
GGGGA	554395	2.1488578	5.810408	7
GAGGG	542075	2.101105	6.3609223	9
CTCCT	665405	2.0010555	6.1781197	4
CTCCA	666870	1.9722496	6.1209087	6
TCCAG	648730	1.9514785	6.3825893	7
GCCAG	511080	1.9147812	7.2766185	4
CCCAC	524435	1.8991698	5.8115067	9
TGGCT	607090	1.8887924	7.3412366	3
TGTTT	915890	1.8684977	5.680655	3
CACCA	637680	1.8546894	5.5851254	8
TGCAG	604180	1.8486096	5.6076994	3
TGGTG	583945	1.8479156	6.1182337	9
TCCTC	611705	1.8395647	7.010777	5
GGCTC	481060	1.8326596	7.7626367	4
GCCCA	485680	1.7889632	6.440852	4
AGGGG	456740	1.7703431	7.304058	6
GGCCC	353130	1.6472844	5.4516234	1
GCTCC	417780	1.5647722	8.312845	2
TAAAA	1011415	1.549669	8.9551935	1
CTGGT	494225	1.5376441	5.3228226	8
TGCCC	408265	1.5291343	12.435599	3
GATGG	469685	1.4617215	6.054048	1
ATGGA	593545	1.458575	10.803093	2
CATGG	464140	1.4201291	42.27691	1
TGCTC	426385	1.3042294	6.252946	1
ATGTG	514155	1.2847584	8.329331	2
TGGGT	399665	1.2647548	5.858752	3
ATGAA	640620	1.243062	9.140788	2
TGAGA	503430	1.2371268	6.2916646	1
ATGGC	399060	1.2210038	11.332324	2
ATGGG	390110	1.2140734	14.459487	2
ATGCC	403240	1.2130071	11.872516	2
AGACA	507175	1.2050403	5.027166	2
GTCTT	449685	1.1233344	5.0482388	9
GAGAC	368775	1.109655	6.6266665	2
CATGT	435115	1.0689377	33.03415	1
CATGA	433715	1.0478532	29.978933	1
GTGTC	332925	1.0358039	5.664707	2
ATGTT	515270	1.0337888	7.884881	2
TAAAG	527020	1.0226321	6.922952	1
ATGCT	407155	1.0002489	7.8442273	2
AGACT	410250	0.9911619	5.073548	1
CACTC	329620	0.9748421	5.407008	1
AGACC	327490	0.9688253	6.0208397	3
ATGAT	489855	0.96652293	6.860317	2
ATGAG	392830	0.9653388	5.265651	2
ATGAC	398000	0.961566	7.225933	2
ATGGT	383560	0.9584306	7.883427	2
ATGTA	471230	0.92977434	9.441418	2
GCGCC	198955	0.9280873	5.167093	8
CATGC	299715	0.9015882	31.870378	1
CGCTG	227390	0.8662713	6.558356	6
TAACT	387310	0.7513189	5.5576496	1
TAAGT	377450	0.74473894	6.1716175	1
ATGCA	307360	0.7425802	8.933616	2
TAAAC	364190	0.69477034	5.4275093	1
TAAGG	261965	0.6437517	5.031505	1
TGCCG	155400	0.59201616	8.728799	5
CCGTC	129215	0.48396775	5.7628045	7
CGTCT	147545	0.45131168	5.1121407	8
GCCGT	118440	0.45121232	5.82433	6
GTCGC	97390	0.37101966	5.9558463	4
>>END_MODULE