FastQCFastQC Report
Wed 20 Mar 2013
8016-8-41.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-41.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4201668
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 16685 0.39710419766626015 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10410 0.2477587472403817 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10281 0.24468853798063056 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 9196 0.21886546009822766 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 9148 0.2177230566527389 No Hit
TGAGACCGCACCAGCGACACA 9010 0.2144386467469586 No Hit
TGTGTCGCTGGTGCGGTCTCA 8887 0.21151123791789359 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8808 0.20963103224719326 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8734 0.20786982693539804 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8073 0.19213797948814615 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7978 0.18987697266894957 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 7156 0.170313313664954 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 7087 0.1686711087120639 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 7065 0.1681475071328815 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 7050 0.16779050605616627 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 6820 0.16231648954653247 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 6741 0.16043628387583217 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 6707 0.1596270814352776 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 6694 0.1593176805021244 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6671 0.15877027885116102 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 6615 0.1574374748314241 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 6599 0.15705667368292783 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6589 0.15681867296511764 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 6544 0.15574766973497192 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6481 0.15424826521276788 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6392 0.15213005882425742 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6271 0.14925025013875443 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 6246 0.148655248344229 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6242 0.14856004805710493 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 6223 0.14810784669326563 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 6111 0.14544223865379177 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 6105 0.1452994382231057 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6092 0.14499003728995247 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6059 0.14420463492117894 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5576 0.13270920025094796 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5565 0.13244739946135678 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 5545 0.13197139802573643 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 5469 0.13016259257037918 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5302 0.12618798058294944 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5249 0.12492657677855556 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5160 0.1228083703900451 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5141 0.12235616902620577 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5104 0.12147556637030818 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5079 0.12088056457578275 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 5061 0.12045216328372448 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 4965 0.11816735639274688 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 4952 0.11785795545959366 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 4908 0.11681075230122893 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 4859 0.11564454878395913 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 4847 0.11535894792258693 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 4833 0.1150257469176527 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 4822 0.11476394612806153 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 4756 0.11319314139051444 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 4754 0.1131455412469524 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 4640 0.11043233306391653 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4638 0.11038473292035449 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 4624 0.11005153191542026 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 4505 0.1072193233734793 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 4484 0.10671952186607796 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 4467 0.10631492064580067 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 4375 0.10412531404194714 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 4313 0.10264970959152414 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2453620 3.6971283 6.855226 2
TTTTT 2131655 3.49169 4.2778673 95-96
GGGGG 671515 3.2963011 8.631456 4
GGAGG 701600 2.7194304 5.4914117 4
CCAGC 724165 2.6674035 8.436196 5
CCTCC 703910 2.5920389 9.547411 6
CCCCA 706430 2.55824 5.424792 1
CCTGG 668765 2.5477457 5.728869 9
CAGCC 690670 2.544027 6.063372 6
TGGGG 608160 2.3969474 10.371142 3
CTCCC 630005 2.3198955 5.3298492 3
GCTGG 571530 2.2146273 8.475924 7
GGGGA 554395 2.1488578 5.810408 7
GAGGG 542075 2.101105 6.3609223 9
CTCCT 665405 2.0010555 6.1781197 4
CTCCA 666870 1.9722496 6.1209087 6
TCCAG 648730 1.9514785 6.3825893 7
GCCAG 511080 1.9147812 7.2766185 4
CCCAC 524435 1.8991698 5.8115067 9
TGGCT 607090 1.8887924 7.3412366 3
TGTTT 915890 1.8684977 5.680655 3
CACCA 637680 1.8546894 5.5851254 8
TGCAG 604180 1.8486096 5.6076994 3
TGGTG 583945 1.8479156 6.1182337 9
TCCTC 611705 1.8395647 7.010777 5
GGCTC 481060 1.8326596 7.7626367 4
GCCCA 485680 1.7889632 6.440852 4
AGGGG 456740 1.7703431 7.304058 6
GGCCC 353130 1.6472844 5.4516234 1
GCTCC 417780 1.5647722 8.312845 2
TAAAA 1011415 1.549669 8.9551935 1
CTGGT 494225 1.5376441 5.3228226 8
TGCCC 408265 1.5291343 12.435599 3
GATGG 469685 1.4617215 6.054048 1
ATGGA 593545 1.458575 10.803093 2
CATGG 464140 1.4201291 42.27691 1
TGCTC 426385 1.3042294 6.252946 1
ATGTG 514155 1.2847584 8.329331 2
TGGGT 399665 1.2647548 5.858752 3
ATGAA 640620 1.243062 9.140788 2
TGAGA 503430 1.2371268 6.2916646 1
ATGGC 399060 1.2210038 11.332324 2
ATGGG 390110 1.2140734 14.459487 2
ATGCC 403240 1.2130071 11.872516 2
AGACA 507175 1.2050403 5.027166 2
GTCTT 449685 1.1233344 5.0482388 9
GAGAC 368775 1.109655 6.6266665 2
CATGT 435115 1.0689377 33.03415 1
CATGA 433715 1.0478532 29.978933 1
GTGTC 332925 1.0358039 5.664707 2
ATGTT 515270 1.0337888 7.884881 2
TAAAG 527020 1.0226321 6.922952 1
ATGCT 407155 1.0002489 7.8442273 2
AGACT 410250 0.9911619 5.073548 1
CACTC 329620 0.9748421 5.407008 1
AGACC 327490 0.9688253 6.0208397 3
ATGAT 489855 0.96652293 6.860317 2
ATGAG 392830 0.9653388 5.265651 2
ATGAC 398000 0.961566 7.225933 2
ATGGT 383560 0.9584306 7.883427 2
ATGTA 471230 0.92977434 9.441418 2
GCGCC 198955 0.9280873 5.167093 8
CATGC 299715 0.9015882 31.870378 1
CGCTG 227390 0.8662713 6.558356 6
TAACT 387310 0.7513189 5.5576496 1
TAAGT 377450 0.74473894 6.1716175 1
ATGCA 307360 0.7425802 8.933616 2
TAAAC 364190 0.69477034 5.4275093 1
TAAGG 261965 0.6437517 5.031505 1
TGCCG 155400 0.59201616 8.728799 5
CCGTC 129215 0.48396775 5.7628045 7
CGTCT 147545 0.45131168 5.1121407 8
GCCGT 118440 0.45121232 5.82433 6
GTCGC 97390 0.37101966 5.9558463 4
Produced by FastQC (version 0.10.0)