##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-42.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10161160	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.996548228745535	24.0	24.0	31.0	23.0	31.0
2	31.03246109138028	31.0	31.0	32.0	29.0	33.0
3	32.69515260188723	34.0	33.0	34.0	29.0	35.0
4	33.912772899397545	34.0	33.0	36.0	29.0	37.0
5	35.44706096868842	36.0	35.0	37.0	33.0	37.0
6	35.76829702855009	36.0	36.0	38.0	33.0	38.0
7	35.78835058018739	37.0	35.0	37.0	34.0	37.0
8	35.63350972892258	36.0	36.0	37.0	32.0	38.0
9	35.66105732998119	37.0	36.0	37.0	31.0	38.0
10-14	36.02894823948318	37.0	36.0	37.4	32.4	38.0
15-19	37.018975801429576	38.0	37.2	38.4	33.4	39.4
20-24	36.6431048092046	37.4	36.0	38.8	32.8	39.8
25-29	36.29830740089302	37.6	36.2	38.0	32.8	38.2
30-34	36.14822350879981	37.6	35.6	38.2	32.6	38.6
35-39	36.18044852345825	37.8	36.4	38.0	31.6	38.6
40-44	36.107848186612884	37.4	36.0	38.4	32.4	38.8
45-49	36.06381060023042	37.6	35.8	38.2	32.4	38.6
50-54	36.28200838063252	37.6	36.2	38.4	33.4	39.0
55-59	35.994381560493466	37.6	35.8	38.2	32.4	38.6
60-64	36.00259992554423	37.6	35.6	38.2	32.6	38.6
65-69	35.80774631417593	37.4	36.0	38.2	31.6	38.6
70-74	35.85740408437854	37.4	36.0	38.4	32.0	39.0
75-79	35.574117749158845	37.4	35.4	37.6	32.2	38.4
80-84	35.685796531160136	37.2	35.8	38.2	31.2	38.6
85-89	35.39377971291123	37.2	35.4	37.8	31.0	38.2
90-94	35.40891318953884	37.0	35.0	38.2	31.6	38.4
95-99	35.234503129591005	37.4	35.2	37.6	31.0	38.2
100	34.148314017792124	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	9.0
2	34.0
3	159.0
4	0.0
5	21.0
6	59.0
7	272.0
8	432.0
9	10854.0
10	911.0
11	1373.0
12	1941.0
13	3334.0
14	4298.0
15	5349.0
16	6683.0
17	8683.0
18	11433.0
19	13817.0
20	15689.0
21	16916.0
22	18709.0
23	22049.0
24	27559.0
25	36414.0
26	48544.0
27	66198.0
28	89981.0
29	119344.0
30	157739.0
31	206598.0
32	265137.0
33	355376.0
34	524017.0
35	945725.0
36	2885668.0
37	4289823.0
38	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.97268040668174	19.076707725880865	21.66691500868803	39.283696858749366
2	17.340523486646305	52.29035384047693	15.739903133934014	14.62921953894275
3	18.945939418972088	34.83038137869857	30.679659806027125	15.544019396302215
4	33.85220668253057	24.963727744286977	21.164007555311557	20.02005801787089
5	20.350107335288907	27.883382064660232	26.79131065944464	24.975199940606227
6	19.772006540533454	29.737720338224104	26.39823079004955	24.092042331192893
7	21.278113341790647	28.669919373318752	27.68800521482729	22.363962070063312
8	20.961532451307313	28.165088907447398	29.279275447919705	21.59410319332558
9	21.134969780227934	29.241355641864793	27.26774886187522	22.355925716032058
10-14	21.580096863209967	27.8866716386872	27.178824343504676	23.354407154598157
15-19	21.019980565678367	27.661244087830216	27.858921197292236	23.459854149199177
20-24	21.40140067374838	26.81962001314771	28.140506464728148	23.63847284837576
25-29	21.58708894629137	27.337335486065374	28.57548808162364	22.500087486019616
30-34	22.050877014258255	27.877668939016907	27.739683571098126	22.331770475626705
35-39	22.194312732494563	26.6318281300451	28.871346917463082	22.302512219997258
40-44	22.124720709028857	27.75076643008848	27.723288841478034	22.401224019404626
45-49	21.847409320753542	28.267309003496617	28.174927203368576	21.710354472381262
50-54	22.268154488607397	27.773642941709	27.938056354077258	22.020146215606353
55-59	21.712526222086225	28.66222280685486	27.53712997588846	22.08812099517046
60-64	22.160324593085107	28.001550752050115	28.35447731537883	21.483647339485948
65-69	22.126965094455617	28.86748371376051	27.932251682479553	21.07329950930432
70-74	21.97738392678693	28.417565610685884	27.739038455171872	21.866012007355312
75-79	22.357357683143185	28.518393581973267	27.731794054653758	21.39245468022979
80-84	21.64902182535949	28.44728025467146	27.97289809011932	21.930799829849732
85-89	21.92795261380324	28.198345123485037	28.064741489649126	21.808960773062598
90-94	21.772773884280664	27.956854193261904	28.308220261313156	21.962151661144276
95-99	22.040985945404234	27.13335426805826	28.515202174399473	22.310457612138034
100	23.377060215731635	27.075997585173393	27.139092944111727	22.40784925498325
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	59.256377265431105
2	31.96974302558906
3	34.4899588152743
4	53.87226470040146
5	45.325307275895135
6	43.86404887172635
7	43.64207541185396
8	42.55563564463289
9	43.49089549625999
10-14	44.93450401780812
15-19	44.47983471487754
20-24	45.03987352212414
25-29	44.08717643231098
30-34	44.38264748988496
35-39	44.49682495249182
40-44	44.52594472843348
45-49	43.557763793134804
50-54	44.288300704213746
55-59	43.80064721725668
60-64	43.64397193257105
65-69	43.20026460375993
70-74	43.843395934142244
75-79	43.749812363372975
80-84	43.57982165520922
85-89	43.73691338686584
90-94	43.73492554542494
95-99	44.35144355754227
100	45.78490947071489
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11069.0
1	11068.0
2	11072.0
3	11075.0
4	11081.5
5	11096.5
6	11112.5
7	11143.0
8	11171.5
9	11203.5
10	11256.5
11	11315.5
12	11381.0
13	11449.0
14	11512.0
15	12648.0
16	13289.5
17	12804.0
18	13597.0
19	15838.5
20	17927.5
21	20596.0
22	28201.5
23	41421.0
24	64394.5
25	86093.0
26	115277.5
27	146394.0
28	149860.0
29	179853.0
30	235895.0
31	261710.0
32	275634.0
33	299393.0
34	326482.0
35	332952.0
36	318280.5
37	300094.5
38	345093.0
39	382529.0
40	387312.0
41	415704.0
42	397089.5
43	399612.5
44	408783.5
45	380008.0
46	346119.0
47	303475.0
48	287757.5
49	303312.0
50	320859.5
51	325959.5
52	272445.0
53	186788.5
54	159925.0
55	157417.0
56	122743.0
57	103532.0
58	140646.0
59	139208.0
60	131677.5
61	159776.5
62	142525.5
63	125903.5
64	119429.0
65	110797.0
66	111770.5
67	96623.5
68	70682.5
69	61215.0
70	60852.5
71	54162.5
72	62197.0
73	49560.0
74	30583.0
75	34284.0
76	33588.0
77	32038.5
78	28680.5
79	28656.5
80	26139.0
81	22121.5
82	20116.0
83	20761.0
84	22763.0
85	21892.0
86	21777.0
87	19586.0
88	19697.5
89	22132.0
90	24164.5
91	26308.5
92	24543.5
93	22770.0
94	22714.0
95	22704.0
96	22683.5
97	22667.5
98	22661.5
99	22647.0
100	22641.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035478232800192105
2	0.008581334897087036
3	0.0022858532385663247
4	0.007173621647231046
5	0.004965688866915689
6	0.0010378867202987215
7	4.6483034285886094E-4
8	2.1758510989927193E-4
9	9.89027726403282E-5
10-14	0.0014736628556888053
15-19	0.017382686500034352
20-24	0.015997333526620403
25-29	0.008422178445605845
30-34	0.008006488972159378
35-39	0.005677431188887698
40-44	0.013234537211256366
45-49	0.011904357401114465
50-54	0.010794481852888288
55-59	0.009376635337027369
60-64	0.041041287077120174
65-69	0.03595448241350832
70-74	0.023414131838315817
75-79	0.05210413916311238
80-84	0.048579218721026836
85-89	0.011710513852733492
90-94	0.001431659991630103
95-99	0.00873445837880861
100	0.008525961660096618
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	11224.0
2-3	1645.0
4-5	31580.0
6-7	5725.0
8-9	48.0
10-11	58.0
12-13	104.0
14-15	252.0
16-17	359.0
18-19	3173.0
20-21	26322.0
22-23	3956.0
24-25	4422.0
26-27	2669.0
28-29	2294.0
30-31	3520.0
32-33	2242.0
34-35	3158.0
36-37	2152.0
38-39	1998.0
40-41	3684.0
42-43	2652.0
44-45	3455.0
46-47	6290.0
48-49	2290.0
50-51	27895.0
52-53	3426.0
54-55	31314.0
56-57	22484.0
58-59	68470.0
60-61	3449.0
62-63	18153.0
64-65	9626.0
66-67	13019.0
68-69	8855.0
70-71	42175.0
72-73	39324.0
74-75	23989.0
76-77	27842.0
78-79	9842.0
80-81	21669.0
82-83	23857.0
84-85	14715.0
86-87	4942.0
88-89	9560.0
90-91	20894.0
92-93	2647.0
94-95	6943.0
96-97	221150.0
98-99	0.0
100-101	9359648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.37608285901335
#Duplication Level	Relative count
1	100.0
2	7.618371093062436
3	2.6163594759737485
4	1.4049888103799442
5	1.020895672508738
6	0.8769393245995625
7	0.8285347884030275
8	0.8046468354748674
9	0.7914455983303578
10++	9.763886444215343
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	24116	0.2373351074089966	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	24059	0.23677414783351505	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	21756	0.21410941270484865	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	21426	0.21086175200469237	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	19846	0.19531234622818655	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	19755	0.1944167791866283	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	19452	0.19143483618012117	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	19320	0.19013577190005865	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	17472	0.1719488719791835	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	17135	0.16863232150659965	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	16578	0.16315066390057828	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	16475	0.16213700010628707	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	16378	0.16118238468836235	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	16204	0.1594699817737345	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	16136	0.15880076684158106	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	15831	0.15579914104295178	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	14621	0.14389105180904543	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	14584	0.1435269201547855	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	14292	0.1406532325049502	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	14189	0.139639568710659	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	13810	0.13590967960350983	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	13552	0.1333705994197513	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	12702	0.1250054127678336	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	12673	0.12472001228206228	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	11905	0.11716182010715313	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	11841	0.11653197075924403	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	11806	0.11618752189710624	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	11772	0.11585291443102952	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	11666	0.1148097264485551	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	11272	0.11093221640048971	No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC	11188	0.11010553913135901	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	11063	0.10887536462372406	No Hit
GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG	11013	0.10838329482067008	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	10938	0.10764519011608911	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	10934	0.10760582453184478	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	10332	0.10168130410307485	No Hit
*	10259	0.10096288219061604	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	10247	0.10084478543788307	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	5230910	3.3455017	4.0972943	60-64
AAAAA	5562830	3.3214219	6.371845	2
CCCAG	1954830	3.0318513	4.5439067	40-44
GGGGG	1424880	3.006446	8.498901	4
GGAGG	1689545	2.7694623	5.117189	4
CCAGG	1702615	2.6898267	5.02452	5
CCCCA	1724410	2.6256115	5.718414	1
CCTCC	1690945	2.610301	9.574435	6
CCAGC	1679040	2.6041138	8.0605755	5
CAGCC	1661795	2.5773678	5.9668827	6
TGGGG	1455140	2.4182525	10.607896	3
GGCAG	1380560	2.2216318	5.198036	8
GAGGG	1317820	2.1601396	6.746403	9
GGGGA	1291505	2.1170046	5.371059	7
CCCTC	1328820	2.051291	5.7425213	5
CTCCT	1613185	1.9979103	5.0409985	4
CTCCA	1629565	1.9906384	5.124534	6
GCCAG	1229940	1.9430848	7.4988227	4
GCCCC	989925	1.9401782	5.177786	9
TCCAG	1545355	1.9229053	5.9052978	7
CCCAC	1258950	1.9168955	5.7592926	9
TGGCT	1482555	1.9051121	6.5294447	3
CACCA	1552910	1.871095	5.0534186	8
TGCAG	1467780	1.8603709	5.6402383	3
TGTTT	2276455	1.8485078	5.661747	3
GCCCA	1181220	1.8320179	5.568552	4
TCCTC	1478720	1.8313771	5.9040337	5
GGCTC	1135010	1.8179364	7.0678334	1
AGGGG	1064800	1.7453952	7.2700353	6
GGCCC	823360	1.6437585	5.3783274	1
GCTCC	993760	1.5626135	7.256787	2
TGCCC	980060	1.5410713	12.018346	3
GATGG	1186000	1.5312011	6.026748	1
TAAAA	2522870	1.5271944	8.037471	1
ATGGA	1476300	1.4807193	10.309353	2
CATGG	1134805	1.4383343	42.099297	1
GCAGT	1058890	1.3421139	5.420497	4
ATGTG	1279655	1.3012544	7.662308	2
TGGGT	985515	1.2899768	6.1077476	3
TGCTC	1018075	1.2843417	5.2962465	1
ATGAA	1599195	1.2460922	8.954929	2
ATGGG	963980	1.244559	14.956986	2
ATGGC	974010	1.2345309	11.285619	2
TGAGA	1207620	1.211235	5.0223722	1
ATGCC	966900	1.2031261	12.484058	2
CATGT	1080075	1.0782372	32.829247	1
ATGTT	1336025	1.0700539	8.102177	2
CATGA	1083135	1.0665269	29.61735	1
TAAAG	1334325	1.0397058	6.3925967	1
ATGCT	996570	0.9948741	7.8505797	2
ATGGT	967800	0.9841355	7.6933575	2
ATGAC	980630	0.9655935	7.144355	2
ATGAT	1206740	0.9533088	6.647371	2
ATGAG	944065	0.946891	5.0448914	2
ATGTA	1195825	0.9446862	9.373566	2
CATGC	741080	0.92213553	32.842697	1
GCGCC	427765	0.8539914	5.1313586	8
ATGCA	767330	0.75556415	9.030243	2
TAAGT	950925	0.7512184	6.1574116	1
TAACT	956475	0.7417962	5.174584	1
TAAAC	896700	0.68594146	5.0500712	1
TAAGG	667020	0.6690168	5.2968163	1
TGCCG	352380	0.56440425	6.224759	5
>>END_MODULE