FastQCFastQC Report
Wed 20 Mar 2013
8016-8-42.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-42.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10161160
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 24116 0.2373351074089966 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 24059 0.23677414783351505 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 21756 0.21410941270484865 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 21426 0.21086175200469237 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 19846 0.19531234622818655 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 19755 0.1944167791866283 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 19452 0.19143483618012117 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 19320 0.19013577190005865 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 17472 0.1719488719791835 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 17135 0.16863232150659965 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 16578 0.16315066390057828 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 16475 0.16213700010628707 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 16378 0.16118238468836235 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 16204 0.1594699817737345 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 16136 0.15880076684158106 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 15831 0.15579914104295178 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 14621 0.14389105180904543 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 14584 0.1435269201547855 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 14292 0.1406532325049502 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 14189 0.139639568710659 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 13810 0.13590967960350983 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 13552 0.1333705994197513 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 12702 0.1250054127678336 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 12673 0.12472001228206228 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 11905 0.11716182010715313 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 11841 0.11653197075924403 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 11806 0.11618752189710624 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 11772 0.11585291443102952 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 11666 0.1148097264485551 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 11272 0.11093221640048971 No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC 11188 0.11010553913135901 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 11063 0.10887536462372406 No Hit
GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG 11013 0.10838329482067008 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 10938 0.10764519011608911 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 10934 0.10760582453184478 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 10332 0.10168130410307485 No Hit
* 10259 0.10096288219061604 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 10247 0.10084478543788307 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 5230910 3.3455017 4.0972943 60-64
AAAAA 5562830 3.3214219 6.371845 2
CCCAG 1954830 3.0318513 4.5439067 40-44
GGGGG 1424880 3.006446 8.498901 4
GGAGG 1689545 2.7694623 5.117189 4
CCAGG 1702615 2.6898267 5.02452 5
CCCCA 1724410 2.6256115 5.718414 1
CCTCC 1690945 2.610301 9.574435 6
CCAGC 1679040 2.6041138 8.0605755 5
CAGCC 1661795 2.5773678 5.9668827 6
TGGGG 1455140 2.4182525 10.607896 3
GGCAG 1380560 2.2216318 5.198036 8
GAGGG 1317820 2.1601396 6.746403 9
GGGGA 1291505 2.1170046 5.371059 7
CCCTC 1328820 2.051291 5.7425213 5
CTCCT 1613185 1.9979103 5.0409985 4
CTCCA 1629565 1.9906384 5.124534 6
GCCAG 1229940 1.9430848 7.4988227 4
GCCCC 989925 1.9401782 5.177786 9
TCCAG 1545355 1.9229053 5.9052978 7
CCCAC 1258950 1.9168955 5.7592926 9
TGGCT 1482555 1.9051121 6.5294447 3
CACCA 1552910 1.871095 5.0534186 8
TGCAG 1467780 1.8603709 5.6402383 3
TGTTT 2276455 1.8485078 5.661747 3
GCCCA 1181220 1.8320179 5.568552 4
TCCTC 1478720 1.8313771 5.9040337 5
GGCTC 1135010 1.8179364 7.0678334 1
AGGGG 1064800 1.7453952 7.2700353 6
GGCCC 823360 1.6437585 5.3783274 1
GCTCC 993760 1.5626135 7.256787 2
TGCCC 980060 1.5410713 12.018346 3
GATGG 1186000 1.5312011 6.026748 1
TAAAA 2522870 1.5271944 8.037471 1
ATGGA 1476300 1.4807193 10.309353 2
CATGG 1134805 1.4383343 42.099297 1
GCAGT 1058890 1.3421139 5.420497 4
ATGTG 1279655 1.3012544 7.662308 2
TGGGT 985515 1.2899768 6.1077476 3
TGCTC 1018075 1.2843417 5.2962465 1
ATGAA 1599195 1.2460922 8.954929 2
ATGGG 963980 1.244559 14.956986 2
ATGGC 974010 1.2345309 11.285619 2
TGAGA 1207620 1.211235 5.0223722 1
ATGCC 966900 1.2031261 12.484058 2
CATGT 1080075 1.0782372 32.829247 1
ATGTT 1336025 1.0700539 8.102177 2
CATGA 1083135 1.0665269 29.61735 1
TAAAG 1334325 1.0397058 6.3925967 1
ATGCT 996570 0.9948741 7.8505797 2
ATGGT 967800 0.9841355 7.6933575 2
ATGAC 980630 0.9655935 7.144355 2
ATGAT 1206740 0.9533088 6.647371 2
ATGAG 944065 0.946891 5.0448914 2
ATGTA 1195825 0.9446862 9.373566 2
CATGC 741080 0.92213553 32.842697 1
GCGCC 427765 0.8539914 5.1313586 8
ATGCA 767330 0.75556415 9.030243 2
TAAGT 950925 0.7512184 6.1574116 1
TAACT 956475 0.7417962 5.174584 1
TAAAC 896700 0.68594146 5.0500712 1
TAAGG 667020 0.6690168 5.2968163 1
TGCCG 352380 0.56440425 6.224759 5
Produced by FastQC (version 0.10.0)