##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-43.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3029814	
Filtered Sequences	0	
Sequence length	1-100	
%GC	45	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.708270870753122	24.0	24.0	31.0	20.0	31.0
2	30.866871757289072	31.0	31.0	32.0	27.0	33.0
3	32.401713429956395	33.0	33.0	34.0	29.0	35.0
4	33.59903037739592	34.0	33.0	36.0	29.0	37.0
5	35.14995099117307	36.0	35.0	37.0	33.0	37.0
6	35.504025882776084	36.0	36.0	38.0	33.0	38.0
7	35.50849141416424	37.0	35.0	37.0	32.0	37.0
8	35.364857202662535	36.0	36.0	37.0	32.0	38.0
9	35.347427395939846	37.0	36.0	37.0	31.0	38.0
10-14	35.692244730621475	37.0	36.0	37.4	31.6	38.0
15-19	36.65922764021341	38.0	37.0	38.4	32.6	39.4
20-24	36.31008990593829	37.4	36.0	38.8	32.8	39.8
25-29	35.95010700138904	37.6	36.2	38.0	32.0	38.2
30-34	35.73223178832377	37.6	35.4	38.2	31.2	38.6
35-39	35.71219683340467	37.6	36.2	38.0	30.4	38.6
40-44	35.69042584027359	37.4	35.8	38.4	31.2	38.8
45-49	35.643768429338834	37.6	35.6	38.2	30.8	38.6
50-54	35.82642746392078	37.4	35.8	38.2	32.2	39.0
55-59	35.54448904724439	37.2	35.6	38.2	31.0	38.6
60-64	35.60360611654493	37.4	35.6	38.2	31.8	38.6
65-69	35.3806798942325	37.4	35.4	38.2	30.4	38.6
70-74	35.34973534043677	37.2	35.8	38.4	31.0	39.0
75-79	35.04036692934532	37.4	35.4	37.6	29.8	38.4
80-84	35.14235835743703	37.0	35.6	38.2	30.6	38.4
85-89	34.84547047919162	37.2	34.8	37.6	30.0	38.2
90-94	34.86276883548492	37.0	35.0	38.2	29.0	38.4
95-99	34.692489572672734	37.4	34.8	37.6	28.8	38.2
100	33.623435832767484	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	3.0
1	22.0
2	103.0
3	448.0
4	0.0
5	37.0
6	32.0
7	183.0
8	241.0
9	25871.0
10	945.0
11	1606.0
12	2556.0
13	4725.0
14	5226.0
15	5869.0
16	6091.0
17	6802.0
18	7486.0
19	8217.0
20	9551.0
21	8538.0
22	8108.0
23	8523.0
24	10368.0
25	13540.0
26	17784.0
27	24106.0
28	33114.0
29	42324.0
30	53409.0
31	67684.0
32	87176.0
33	113902.0
34	166496.0
35	295528.0
36	844009.0
37	1149189.0
38	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.069538542074948	18.549594247502196	21.04335119694406	40.3375160134788
2	18.64226769332557	51.14909463302762	14.362042181942233	15.846595491704566
3	20.19826513837428	33.286690816779576	29.776702562960732	16.738341481885417
4	34.05347420257534	24.632232308870176	20.762451882421047	20.551841606133443
5	20.84874407850252	26.612913453522125	26.016441688143253	26.521900779832098
6	20.95765028509694	28.29890487208577	24.610059987685577	26.133384855131712
7	23.412146608428664	27.229637764745473	25.945164978847146	23.413050647978718
8	21.951785592880604	26.99446828077971	27.704545842737343	23.34920028360234
9	22.033935203528497	28.704589702617334	26.206188129008833	23.055286964845337
10-14	22.871598096310557	26.76474282473939	26.08781368790091	24.27584539104915
15-19	22.20501584165185	26.51419314147295	26.734749687155023	24.546041329720172
20-24	22.37002835253812	26.310526783465065	26.8262786423121	24.49316622168471
25-29	22.29517975355054	27.045178984869278	27.431425944734894	23.22821531684529
30-34	22.94949387633111	27.175146808346202	26.89610090805779	22.9792584072649
35-39	22.772454074485022	26.082199771394453	28.105538553726966	23.03980760039356
40-44	23.22532544286002	26.924086155569594	26.831163828781886	23.019424572788502
45-49	22.548406234533747	27.407432507727187	27.348160551379337	22.696000706359733
50-54	22.92522203720467	26.87473484635687	27.40889576808559	22.791147348352872
55-59	22.079607594328838	27.916876138387174	27.25629183582677	22.747224431457216
60-64	22.627367115947834	27.94027879574894	27.516809051972075	21.915545036331157
65-69	22.845166001552425	28.78820723274166	26.9787381512712	21.387888614434715
70-74	22.5367568130865	28.286303100846265	26.68215201111118	22.494788074956052
75-79	23.039742814740205	28.05667636227961	26.953214715140707	21.950366107839475
80-84	22.30733000896467	28.00375345927215	27.102504256593296	22.586412275169884
85-89	22.698458405049017	27.675806341300003	27.393147443959393	22.23258780969159
90-94	22.663231699493146	27.421569568425998	27.272109609407323	22.643089122673533
95-99	22.89228596189333	26.518650302111073	27.730409931425026	22.85865380457057
100	24.155886991669405	26.39593510889993	26.332878254377324	23.115299645053337
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	60.40705455555374
2	34.48886318503014
3	36.936606620259695
4	54.605315808708774
5	47.37064485833462
6	47.091035140228655
7	46.82519725640738
8	45.30098587648294
9	45.08922216837383
10-14	47.1474434873597
15-19	46.75105717137202
20-24	46.86319457422283
25-29	45.52339507039583
30-34	45.92875228359601
35-39	45.81226167487858
40-44	46.24475001564852
45-49	45.24440694089348
50-54	45.71636938555754
55-59	44.826832025786054
60-64	44.54291215227899
65-69	44.23305461598714
70-74	45.03154488804255
75-79	44.99010892257968
80-84	44.89374228413456
85-89	44.931046214740604
90-94	45.30632082216668
95-99	45.7509397664639
100	47.271186636722746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27396.0
1	27394.0
2	27391.5
3	27399.5
4	27405.0
5	27407.5
6	27419.5
7	27431.5
8	27431.0
9	27430.0
10	27450.5
11	27478.0
12	27491.0
13	27507.0
14	27531.0
15	27888.5
16	28126.0
17	28006.0
18	28136.0
19	28543.5
20	29079.5
21	29811.5
22	31953.5
23	35208.0
24	41268.5
25	48079.5
26	54610.0
27	62396.5
28	64933.5
29	71077.0
30	84975.0
31	92667.5
32	94352.5
33	101104.0
34	109890.5
35	110560.0
36	108197.5
37	105023.5
38	116751.0
39	126241.0
40	128736.0
41	139098.5
42	131882.0
43	131417.5
44	138054.0
45	132147.5
46	123043.0
47	110994.0
48	107769.0
49	115019.0
50	110905.0
51	102331.0
52	87062.0
53	62074.0
54	55415.5
55	55735.0
56	46686.5
57	39971.5
58	51777.5
59	53537.0
60	55566.5
61	67198.5
62	63447.5
63	61300.0
64	55892.0
65	49573.0
66	49598.5
67	43943.5
68	43415.0
69	47092.5
70	45100.0
71	42999.5
72	44118.0
73	29685.5
74	13782.5
75	16921.0
76	18545.0
77	18408.5
78	16086.5
79	13723.5
80	12405.5
81	11242.0
82	10391.5
83	10255.5
84	10369.0
85	9756.5
86	10923.5
87	10606.0
88	9969.0
89	10602.0
90	11202.0
91	11853.5
92	10478.0
93	9133.0
94	9126.5
95	9120.5
96	9110.0
97	9107.5
98	9120.0
99	9128.0
100	9124.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05142229853053686
2	0.007128759046278638
3	0.0018998853735824606
4	0.0067019994298299
5	0.0037711933557580284
6	9.35940864582059E-4
7	3.3482834187982676E-4
8	1.3396408088081383E-4
9	0.0
10-14	0.0015541174163842964
15-19	0.01990953125082719
20-24	0.01867102482275761
25-29	0.008881750087365676
30-34	0.008597294576510343
35-39	0.00579574200209895
40-44	0.013423976086910972
45-49	0.012533283787839326
50-54	0.011538819133299096
55-59	0.01092746207114851
60-64	0.05402058172903724
65-69	0.044056703352544045
70-74	0.02781007738146915
75-79	0.07402763243488868
80-84	0.06410575717345589
85-89	0.015179420973691253
90-94	0.006975864963565304
95-99	0.009672453735762412
100	0.008170605221725602
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	27889.0
2-3	2820.0
4-5	7463.0
6-7	5767.0
8-9	139.0
10-11	101.0
12-13	138.0
14-15	195.0
16-17	307.0
18-19	4322.0
20-21	44942.0
22-23	3233.0
24-25	4271.0
26-27	1795.0
28-29	1839.0
30-31	2535.0
32-33	2979.0
34-35	2136.0
36-37	1939.0
38-39	1496.0
40-41	2742.0
42-43	1575.0
44-45	2724.0
46-47	5473.0
48-49	1514.0
50-51	10633.0
52-53	1903.0
54-55	11441.0
56-57	7948.0
58-59	22617.0
60-61	1737.0
62-63	8552.0
64-65	3166.0
66-67	4470.0
68-69	4423.0
70-71	11473.0
72-73	14043.0
74-75	7473.0
76-77	9199.0
78-79	3487.0
80-81	7219.0
82-83	7610.0
84-85	5663.0
86-87	2061.0
88-89	3530.0
90-91	6055.0
92-93	1192.0
94-95	2147.0
96-97	61098.0
98-99	0.0
100-101	2680340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.82531709324782
#Duplication Level	Relative count
1	100.0
2	4.6590873153916705
3	1.7543720944941652
4	1.2452167863128933
5	1.1906644318649
6	1.104487524113722
7	1.1693178583852504
8	1.153505581733658
9	1.1400651465798046
10++	10.971348154707314
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	25317	0.8355958484580243	No Hit
GAGACCGCACCAGCGACACC	11416	0.37678880617754096	No Hit
GGTGTCGCTGGTGCGGTCTC	11078	0.36563300585448477	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	7218	0.23823244595212778	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	7191	0.23734130213933924	No Hit
TGAGACCGCACCAGCGACACA	6967	0.2299481090258346	No Hit
TGTGTCGCTGGTGCGGTCTCA	6882	0.22714265628187078	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	6068	0.2002763205926172	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	5992	0.1977679157862496	No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTATGCGATCTCGTATGCCGT	5447	0.17978001289848156	TruSeq Adapter, Index 9 (96% over 33bp)
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	5390	0.1778987092937058	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	5368	0.17717259211291517	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	5365	0.17707357613371646	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	5252	0.17334397425056455	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	4976	0.16423450416428204	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	4924	0.16251822719150416	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	4823	0.15918468922514717	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	4755	0.1569403270299761	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	4727	0.156016177890788	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	4672	0.15420088493881143	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	4599	0.1517914961116425	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	4547	0.15007521913886462	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	4517	0.1490850593468774	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	4431	0.146246601276514	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	4395	0.1450584095261293	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	4341	0.14327612190055233	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	4332	0.14297907396295614	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	4202	0.13868838153101148	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	4190	0.1382923176142166	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	4148	0.13690609390543446	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	4124	0.13611396607184467	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	3943	0.13014000199352171	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	3927	0.12961191677112852	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	3877	0.1279616504511498	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	3845	0.12690548000636342	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	3835	0.12657542674236769	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	3825	0.12624537347837192	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	3790	0.12509018705438685	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	3783	0.12485914976958983	No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG	3753	0.1238689899776026	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	3610	0.11914922830246345	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	3582	0.11822507916327536	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	3498	0.11545263174571113	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	3487	0.1150895731553158	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	3257	0.10749834808341371	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	3242	0.10700326818742008	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	3177	0.10485792197144775	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	3088	0.10192044792188563	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	3083	0.10175542128988777	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	3080	0.10165640531068904	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	3070	0.1013263520466933	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	3066	0.10119433074109499	No Hit
CATGGGGGCCAGTTGTGGGTGGTGGCCCAGGTGCAGGAGAGGCGGGCAGT	3062	0.1010623094354967	No Hit
AGGGTCAGCCCACACCACCCAGCCCCCACGCCCAACCCTCGTGCCTCCCT	3043	0.1004352082339048	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	3035	0.1001711656227082	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	1920175	4.43602	9.471897	60-64
TTTTT	1434540	3.7442455	4.581586	95-96
GGGGG	598270	3.6499321	8.605971	4
GGAGG	539620	2.7109945	5.50839	4
CCAGC	562290	2.69447	8.152717	5
CCTCC	541110	2.615507	9.817958	6
CAGCC	537345	2.574935	6.9164653	6
CCTGG	510485	2.5464687	6.4353285	9
CCCCA	537165	2.533834	5.92791	1
TGGGG	463065	2.383869	10.392519	3
CTCCC	474005	2.2911484	6.128941	3
GCTGG	441260	2.2361062	12.620841	7
GGGGA	428950	2.155	5.635779	7
GAGGG	425455	2.1374416	5.8215137	9
CTCCT	500695	2.0746217	6.3774824	4
CTCCA	500480	2.02373	6.003948	6
AAGAA	709900	1.9915754	5.0115013	2
TCCAG	477900	1.9631133	5.864582	4
TGTTT	617325	1.9094694	5.5119658	3
CACCA	482430	1.9037079	9.200721	8
TGGCT	441215	1.8866917	7.61612	3
CCCAC	396820	1.8718197	5.179071	9
TGGTG	430425	1.8697808	10.133476	9
GGCTC	367890	1.8351573	8.821269	1
GCCAG	376660	1.8336021	6.757123	4
TCCTC	442280	1.83258	6.8394704	5
CCCTG	372605	1.8296226	5.0927434	1
GCCCA	375705	1.8003627	5.9362335	4
AGGGG	352560	1.7712247	7.1655965	6
TAAAA	682985	1.616827	9.047799	1
ACCAG	402635	1.6140615	6.6214795	9
GCTCC	323765	1.5898008	9.697354	2
GGCCC	270945	1.5766714	6.083413	1
TGCCC	318470	1.5638006	12.384692	3
CTGGT	362345	1.5494336	9.458056	8
GATGG	348655	1.4780506	5.664377	1
CTCAG	351355	1.4432929	5.5302672	3
ATGGA	410260	1.4322062	9.910378	2
CATGG	332260	1.3865288	39.381584	1
TGCTC	301735	1.2700881	6.614184	1
TGGGT	291810	1.2676325	5.670247	3
ATGCC	303765	1.2478031	12.617171	2
ATGGG	293200	1.2429607	14.239373	2
ATGTG	346400	1.2391506	7.704427	2
ATGAA	427940	1.2302186	8.356629	2
TGAGA	349745	1.22095	6.2038336	1
AACTG	353185	1.2136854	5.0089593	4
ATGGC	286375	1.1950498	11.43025	2
GTCTT	327975	1.1834346	7.1237993	9
GAGAC	288050	1.1730566	7.5831537	1
GGCAC	240640	1.171449	5.250113	1
GGTGT	265705	1.1542315	5.5848446	1
GCACC	232985	1.1164544	7.320521	7
ATGTT	354315	1.069519	7.685122	2
CATGT	300430	1.0579057	30.73554	1
GTGTC	246960	1.0560324	9.746458	2
CATGA	306355	1.0527588	26.862904	1
TAAAG	353940	1.0174874	6.4687724	1
AGACC	251760	1.0092419	6.347802	3
CACTC	239105	0.96683973	6.3723783	1
ATGCT	274265	0.9657708	7.640661	2
ATGAT	324980	0.95731723	6.4365463	2
ATGAC	273395	0.93949497	6.356692	2
ATGGT	262475	0.938932	7.0349774	2
CATGC	227230	0.93341327	31.058434	1
ATGTA	315875	0.93049586	9.096768	2
CGCTG	182845	0.9120917	11.148011	6
GTATG	219495	0.7851829	5.511856	2
TAAGT	252390	0.7434836	6.926269	1
TAATG	251100	0.73968345	5.2606626	1
TAACT	253700	0.73566	5.8550334	1
ATGCA	212055	0.7287061	8.415546	2
TAAAC	240700	0.68113464	5.475999	1
TGCCG	134285	0.66985816	9.877153	5
CGCAC	133120	0.6379055	6.185744	6
TAAGG	178075	0.6216549	5.026886	1
CCGCA	129185	0.61904913	8.210115	5
TATGC	172655	0.60797095	5.099119	3
CCGTC	115725	0.5682508	7.0037107	7
CGTCT	133480	0.56185514	7.3089294	8
GACCG	112840	0.54931146	6.1165833	3
GCCGT	104800	0.52277726	7.087295	6
TCGCT	115190	0.48486733	8.8756275	5
ACCGC	100890	0.48346063	6.145955	4
GTCGC	87050	0.4342343	10.490671	4
TGTCG	92100	0.39383137	8.9947815	3
>>END_MODULE