FastQCFastQC Report
Wed 20 Mar 2013
8016-8-43.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-43.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3029814
Filtered Sequences 0
Sequence length 1-100
%GC 45

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 25317 0.8355958484580243 No Hit
GAGACCGCACCAGCGACACC 11416 0.37678880617754096 No Hit
GGTGTCGCTGGTGCGGTCTC 11078 0.36563300585448477 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 7218 0.23823244595212778 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 7191 0.23734130213933924 No Hit
TGAGACCGCACCAGCGACACA 6967 0.2299481090258346 No Hit
TGTGTCGCTGGTGCGGTCTCA 6882 0.22714265628187078 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 6068 0.2002763205926172 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 5992 0.1977679157862496 No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTATGCGATCTCGTATGCCGT 5447 0.17978001289848156 TruSeq Adapter, Index 9 (96% over 33bp)
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 5390 0.1778987092937058 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 5368 0.17717259211291517 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 5365 0.17707357613371646 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 5252 0.17334397425056455 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 4976 0.16423450416428204 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 4924 0.16251822719150416 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 4823 0.15918468922514717 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 4755 0.1569403270299761 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 4727 0.156016177890788 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 4672 0.15420088493881143 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 4599 0.1517914961116425 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 4547 0.15007521913886462 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 4517 0.1490850593468774 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 4431 0.146246601276514 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 4395 0.1450584095261293 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 4341 0.14327612190055233 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 4332 0.14297907396295614 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 4202 0.13868838153101148 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 4190 0.1382923176142166 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 4148 0.13690609390543446 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 4124 0.13611396607184467 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 3943 0.13014000199352171 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 3927 0.12961191677112852 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 3877 0.1279616504511498 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 3845 0.12690548000636342 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 3835 0.12657542674236769 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 3825 0.12624537347837192 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 3790 0.12509018705438685 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 3783 0.12485914976958983 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 3753 0.1238689899776026 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 3610 0.11914922830246345 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 3582 0.11822507916327536 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 3498 0.11545263174571113 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 3487 0.1150895731553158 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 3257 0.10749834808341371 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 3242 0.10700326818742008 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 3177 0.10485792197144775 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 3088 0.10192044792188563 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 3083 0.10175542128988777 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 3080 0.10165640531068904 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 3070 0.1013263520466933 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 3066 0.10119433074109499 No Hit
CATGGGGGCCAGTTGTGGGTGGTGGCCCAGGTGCAGGAGAGGCGGGCAGT 3062 0.1010623094354967 No Hit
AGGGTCAGCCCACACCACCCAGCCCCCACGCCCAACCCTCGTGCCTCCCT 3043 0.1004352082339048 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 3035 0.1001711656227082 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1920175 4.43602 9.471897 60-64
TTTTT 1434540 3.7442455 4.581586 95-96
GGGGG 598270 3.6499321 8.605971 4
GGAGG 539620 2.7109945 5.50839 4
CCAGC 562290 2.69447 8.152717 5
CCTCC 541110 2.615507 9.817958 6
CAGCC 537345 2.574935 6.9164653 6
CCTGG 510485 2.5464687 6.4353285 9
CCCCA 537165 2.533834 5.92791 1
TGGGG 463065 2.383869 10.392519 3
CTCCC 474005 2.2911484 6.128941 3
GCTGG 441260 2.2361062 12.620841 7
GGGGA 428950 2.155 5.635779 7
GAGGG 425455 2.1374416 5.8215137 9
CTCCT 500695 2.0746217 6.3774824 4
CTCCA 500480 2.02373 6.003948 6
AAGAA 709900 1.9915754 5.0115013 2
TCCAG 477900 1.9631133 5.864582 4
TGTTT 617325 1.9094694 5.5119658 3
CACCA 482430 1.9037079 9.200721 8
TGGCT 441215 1.8866917 7.61612 3
CCCAC 396820 1.8718197 5.179071 9
TGGTG 430425 1.8697808 10.133476 9
GGCTC 367890 1.8351573 8.821269 1
GCCAG 376660 1.8336021 6.757123 4
TCCTC 442280 1.83258 6.8394704 5
CCCTG 372605 1.8296226 5.0927434 1
GCCCA 375705 1.8003627 5.9362335 4
AGGGG 352560 1.7712247 7.1655965 6
TAAAA 682985 1.616827 9.047799 1
ACCAG 402635 1.6140615 6.6214795 9
GCTCC 323765 1.5898008 9.697354 2
GGCCC 270945 1.5766714 6.083413 1
TGCCC 318470 1.5638006 12.384692 3
CTGGT 362345 1.5494336 9.458056 8
GATGG 348655 1.4780506 5.664377 1
CTCAG 351355 1.4432929 5.5302672 3
ATGGA 410260 1.4322062 9.910378 2
CATGG 332260 1.3865288 39.381584 1
TGCTC 301735 1.2700881 6.614184 1
TGGGT 291810 1.2676325 5.670247 3
ATGCC 303765 1.2478031 12.617171 2
ATGGG 293200 1.2429607 14.239373 2
ATGTG 346400 1.2391506 7.704427 2
ATGAA 427940 1.2302186 8.356629 2
TGAGA 349745 1.22095 6.2038336 1
AACTG 353185 1.2136854 5.0089593 4
ATGGC 286375 1.1950498 11.43025 2
GTCTT 327975 1.1834346 7.1237993 9
GAGAC 288050 1.1730566 7.5831537 1
GGCAC 240640 1.171449 5.250113 1
GGTGT 265705 1.1542315 5.5848446 1
GCACC 232985 1.1164544 7.320521 7
ATGTT 354315 1.069519 7.685122 2
CATGT 300430 1.0579057 30.73554 1
GTGTC 246960 1.0560324 9.746458 2
CATGA 306355 1.0527588 26.862904 1
TAAAG 353940 1.0174874 6.4687724 1
AGACC 251760 1.0092419 6.347802 3
CACTC 239105 0.96683973 6.3723783 1
ATGCT 274265 0.9657708 7.640661 2
ATGAT 324980 0.95731723 6.4365463 2
ATGAC 273395 0.93949497 6.356692 2
ATGGT 262475 0.938932 7.0349774 2
CATGC 227230 0.93341327 31.058434 1
ATGTA 315875 0.93049586 9.096768 2
CGCTG 182845 0.9120917 11.148011 6
GTATG 219495 0.7851829 5.511856 2
TAAGT 252390 0.7434836 6.926269 1
TAATG 251100 0.73968345 5.2606626 1
TAACT 253700 0.73566 5.8550334 1
ATGCA 212055 0.7287061 8.415546 2
TAAAC 240700 0.68113464 5.475999 1
TGCCG 134285 0.66985816 9.877153 5
CGCAC 133120 0.6379055 6.185744 6
TAAGG 178075 0.6216549 5.026886 1
CCGCA 129185 0.61904913 8.210115 5
TATGC 172655 0.60797095 5.099119 3
CCGTC 115725 0.5682508 7.0037107 7
CGTCT 133480 0.56185514 7.3089294 8
GACCG 112840 0.54931146 6.1165833 3
GCCGT 104800 0.52277726 7.087295 6
TCGCT 115190 0.48486733 8.8756275 5
ACCGC 100890 0.48346063 6.145955 4
GTCGC 87050 0.4342343 10.490671 4
TGTCG 92100 0.39383137 8.9947815 3
Produced by FastQC (version 0.10.0)