FastQCFastQC Report
Wed 20 Mar 2013
8016-8-44.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-44.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2319618
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 5431 0.23413337885807062 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 5422 0.23374538393821742 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 5420 0.23365916284491672 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 5403 0.23292628355186068 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 5391 0.23240895699205644 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 5353 0.23077075621934304 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 5053 0.2178375922242369 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 5012 0.21607005981157243 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 4537 0.19559255015265445 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 4514 0.1946010075796963 No Hit
* 3817 0.1645529565643998 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 3709 0.15989701752616164 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 3674 0.15838814839339926 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 3663 0.15791393238024537 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 3624 0.15623262106088157 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 3511 0.1513611292893916 No Hit
TGAGACCGCACCAGCGACACA 3507 0.1511886871027902 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 3489 0.15041269726308384 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 3476 0.14985226015662925 No Hit
TGTGTCGCTGGTGCGGTCTCA 3460 0.14916249141022359 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 3449 0.1486882753970697 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 3442 0.14838650157051722 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 3342 0.14407544690548185 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 3323 0.14325634651912514 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 3269 0.14092837700000604 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 3251 0.14015238716029965 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 3236 0.13950572896054436 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 3200 0.13795374928113163 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 3187 0.13739331217467704 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 3169 0.13661732233497068 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 3155 0.13601377468186573 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 3129 0.13489290046895655 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 3125 0.13472045828235513 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 3101 0.1336858051627466 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 3100 0.13364269461609626 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 3080 0.1327804836830892 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 3055 0.13170272001683034 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 3025 0.13040940361731976 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 2950 0.12717611261854322 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 2912 0.1255379118458298 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 2903 0.1251499169259766 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 2877 0.12402904271306742 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 2831 0.12204595756715114 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 2815 0.12135618882074548 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 2743 0.11825222946192002 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 2741 0.11816600836861932 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 2731 0.11773490290211577 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 2670 0.11510515955644421 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 2569 0.11075099434475848 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 2557 0.11023366778495425 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 2519 0.10859546701224082 No Hit
GAGACCGCACCAGCGACACC 2512 0.10829369318568834 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 2498 0.10769014553258338 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 2476 0.1067417135062756 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 2453 0.10575017093331747 No Hit
GGTGTCGCTGGTGCGGTCTC 2444 0.10536217601346427 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 2439 0.10514662328021253 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 2430 0.10475862836035935 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 2429 0.10471551781370898 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 2426 0.10458618617375792 No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC 2410 0.10389641742735226 No Hit
TAACTAACTCATAAATGCCTATTTTTTCTTACAGAAAAGGGATTTATAAA 2408 0.10381019633405154 No Hit
AACCTGACAGAAAAACAAAACTCTTCATATTGGTCAATTTCATAATCTTC 2385 0.10281865376109342 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 2349 0.10126667408168069 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 2339 0.10083556861517715 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 2332 0.10053379478862468 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 2332 0.10053379478862468 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 2323 0.10014579986877148 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 1345380 3.5816193 6.9468737 2
TTTTT 1202730 3.4643464 4.30137 95-96
GGGGG 328910 3.0044398 8.809116 4
GGAGG 383715 2.739081 5.6828904 4
CCAGC 396420 2.6789062 8.44761 5
CCAGG 385575 2.6536412 5.6200557 5
CCTCC 384915 2.5946522 10.393175 6
CCTGG 369915 2.5863006 6.2375865 9
CCCCA 386730 2.5661287 5.455783 1
CAGCC 377125 2.5485153 6.463202 6
TGGGG 331640 2.4049535 11.150113 3
GCTGG 311470 2.2178118 7.533718 7
GAGGG 299380 2.1370707 6.4117327 9
GGGGA 296255 2.114763 5.3600225 7
CCACC 308075 2.044217 5.001649 1
CCCTC 299620 2.019692 5.941266 5
CTCCT 365840 1.9938395 5.842682 4
CTCCA 367325 1.970633 5.7161884 7
AAGAA 577390 1.9669534 5.2474365 2
GCCAG 285165 1.9625899 7.7855353 4
TCCAG 354920 1.9391756 6.1704254 7
CCCAC 290920 1.9303861 6.408523 9
TGGAG 338895 1.9205011 5.1609674 3
TGGCT 334310 1.8897794 7.2531247 3
TGTTT 516350 1.873458 5.7918534 3
TGCAG 336420 1.8719741 5.933777 3
TGGTG 321315 1.8497974 5.5466557 9
CACCA 350195 1.8493599 5.8656764 8
GGCTC 262685 1.8365903 7.6321917 4
TCCTC 333230 1.8161141 6.640148 5
GCCCA 264670 1.7885729 5.60985 4
AGGGG 244020 1.741893 6.950291 6
GGCCC 193350 1.6720009 5.7936053 1
GCTCC 223105 1.5316339 7.448322 2
TAAAA 565590 1.529607 9.187943 1
TGCCC 221580 1.5211647 12.491444 3
ATGGA 338745 1.5001403 10.632338 2
GATGG 261000 1.4790739 5.941739 1
CTCAG 265195 1.4489453 5.4520583 3
CATGG 259960 1.4465204 41.94053 1
GCAGT 241930 1.3461941 5.6773953 4
TGCTC 232265 1.2891817 5.5456944 1
TGGGT 221975 1.277901 5.406614 3
ATGAA 367230 1.2708864 9.077477 2
ATGTG 281885 1.2681619 7.4033136 2
TGAGA 278265 1.2323031 5.488616 1
ATGCC 224900 1.2287855 12.7436695 2
ATGGC 219860 1.223388 11.252907 2
AGACA 284875 1.2193776 5.1762195 2
ATGGG 211190 1.1968031 14.357702 2
GTCTT 250005 1.121925 5.499248 9
GAGAC 202645 1.1099672 5.7820606 2
CATGA 247195 1.0748981 30.139126 1
CATGT 239325 1.0572056 31.751059 1
ATGTT 288355 1.0298728 7.931225 2
TAAAG 296200 1.0250701 7.166863 1
GTGTC 179805 1.0163974 5.06435 2
ATGCT 228205 1.0080835 7.867915 2
ATGGT 220065 0.9900422 7.734305 2
CACTC 183780 0.9859469 6.3143435 1
ATGAT 275135 0.96729326 7.0885444 2
ATGAC 220970 0.96086174 7.208327 2
AGACT 220910 0.96060085 5.2455473 1
AGACC 176270 0.9480282 5.1988683 3
ATGTA 262710 0.9236106 8.900789 2
CATGC 167745 0.91650796 33.003223 1
GCGCC 105035 0.9082939 5.7960725 8
CGCTG 122360 0.8554931 5.7581377 6
TAAGA 236240 0.8175644 5.149572 1
GTATG 176575 0.7943867 5.0402865 2
TAACT 219000 0.7560056 5.9049454 1
ATGCA 173570 0.7547485 8.99826 2
TAAGT 209170 0.73537976 6.77982 1
TAAAC 206365 0.7012505 5.790473 1
TAAGG 148510 0.65768 5.7504687 1
TGCCG 83460 0.5835196 9.505755 5
CCGTC 68340 0.46915963 6.3643713 7
GCCGT 64770 0.4528464 6.435949 6
CGTCT 78665 0.43662834 5.43032 8
GTCGC 50165 0.35073403 5.175031 4
Produced by FastQC (version 0.10.0)