FastQCFastQC Report
Wed 20 Mar 2013
8016-8-45.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-45.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4937432
Filtered Sequences 0
Sequence length 1-100
%GC 41

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 13903 0.2815836248478967 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 13824 0.2799836028121501 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 11050 0.22380055056960782 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 11034 0.22347649547376042 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 10459 0.21183076546674465 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 10379 0.2102104899875077 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 9847 0.19943565805058175 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 9826 0.19901033573728205 No Hit
TGTGTCGCTGGTGCGGTCTCACGAGACTGCCCTTAACCTACACA 8928 0.18082274348284696 No Hit
TGTGTAGGTTAAGGGCAGTCTCGTGAGACCGCACCAGCGACACA 8795 0.17812903549861547 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 8383 0.16978461678054504 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 8309 0.16828586196225082 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 8255 0.16719217601376585 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 8178 0.16563266086500025 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 8071 0.1634655424115208 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 8025 0.16253388401095956 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7979 0.16160222561039828 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 7693 0.1558097407721261 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7633 0.15459453416269833 No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG 7631 0.15455402727571743 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 7489 0.1516780383000718 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 7474 0.15137423664771485 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 7411 0.15009826970781573 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 7348 0.14882230276791658 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 7329 0.1484374873415978 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7279 0.1474248151670747 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 7007 0.14191587853766896 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 6989 0.14155131655484066 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 6853 0.13879684824013777 No Hit
CAATTCAAATAAAAACGAAACTGTGTCAATTAGTTGAAGTAATGATGGCA 6583 0.13332841849771299 No Hit
TTAGCTTTGAAGTGGATCCTACCAGTTTTATCATCTTTTCACATAAAAGT 6528 0.13221447910573755 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 6524 0.1321334653317757 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 6497 0.1315866223575332 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 6493 0.13150560858357138 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 6490 0.1314448482531 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6356 0.12873088682537806 No Hit
CATGCTTTACTCAACTGAAATAATCAAGCCCCTATTGCTGTGGGGGAAGC 6295 0.12749542677245987 No Hit
CATGACTATAAATATGAAGCAAATATGATTATCCTCACAAGTTCTGGAAT 6276 0.12711061134614107 No Hit
GTATCTTAATGATGTATATAATTGCCTTCAATCCCCTTCTCACCCCACCC 6251 0.12660427525887952 No Hit
TGTGTCGCTGGTGCGGTCTCACTGCAGCCTGCAGAGCCACA 6216 0.12589540473671332 No Hit
ACTTGAAAGCAAGGATAGAGAAACATTTTAGAGATATCTGGGTTTTTTTC 6201 0.1255916030843564 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 6155 0.12465994468379513 No Hit
AACCTGACAGAAAAACAAAACTCTTCATATTGGTCAATTTCATAATCTTC 6130 0.12415360859653359 No Hit
TAACTAACTCATAAATGCCTATTTTTTCTTACAGAAAAGGGATTTATAAA 6084 0.12322195019597232 No Hit
CATGACTATAAATATCAAGCAAATATGATTTTACTCACAAGTTCTGGAAT 6077 0.1230801760915391 No Hit
TGTGGCTCTGCAGGCTGCAGTGAGACCGCACCAGCGACACA 6034 0.12220927802144921 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6026 0.1220472504735255 No Hit
CATGTCCTCCTTTCTACCAATAACCGCATATCTACTCTTTTTCTGTACTT 6023 0.12198649014305413 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 6019 0.12190547636909228 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 6019 0.12190547636909228 No Hit
ATCATGTCCCCAGAAAATCCAGTTACCTCTTGGTCAGTCATCCGGAAGCA 6012 0.12176370226465905 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATCTGGGTTTTTTTC 5999 0.12150040749928304 No Hit
TAATAGGGTGGTTCTCTTCCCAAAGTGGAAGCCAAATTCATCAATTATGT 5985 0.12121685929041656 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 5970 0.12091305763805961 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 5804 0.11755098601864289 No Hit
GAGTTGACTTTCTAAAAGGTGCTATTTCTTTTTCTTTTCTCTGCTTAGGA 5741 0.11627501907874377 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 5725 0.11595096398289637 No Hit
CATGACAGTTGCTTTGTATATTTTCAAGCACGGCTGGAGGTGCTACTTCT 5709 0.11562690888704896 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 5676 0.11495854525186372 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 5674 0.1149180383648828 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 5607 0.11356105765102183 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 5596 0.11333826977262675 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 5517 0.11173824773688021 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 5424 0.10985467749226724 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5382 0.10900403286566783 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 5367 0.10870023121331089 No Hit
GACTGAACAAGTAGGAAAATGACAGAGTTATCTTCCCAATTGATGTAAGT 5340 0.10815338823906842 No Hit
ATGTCAGATATAAAGAACTCACCGAACAGCAGCTCCCAGGCGCACTTCCT 5319 0.1077280659257687 No Hit
GACTGAAAGAGGGAGAAATAAAGACAAGGCCCCCGAGGAGCTGTCCAAAG 5313 0.10760654526482594 No Hit
TGATCCCTTTTTCCTTTTCTCTTTCCTTGACTCCCGCTTATTCTCCTTTT 5301 0.10736350394294038 No Hit
TAAAACATCGCCTACAGAAAAGCGTATGAAAGGAGTGTAAGCTTTGCTCT 5292 0.10718122295152621 No Hit
CATGGAGGAAGTAGGCAGCTGACAGAAAGTGCTGCAATTGCCTGTGTCAA 5289 0.10712046262105483 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5286 0.10705970229058345 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 5277 0.10687742129916929 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5251 0.10635083176841727 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 5180 0.10491283728059445 No Hit
TGTGTCGCTGGTGCGGTCTCAGCTTGGCGCAATCATGACACA 5147 0.10424447364540919 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 5079 0.10286723948805777 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 5064 0.10256343783570084 No Hit
TGTGTCATGATTGCGCCAAGCTGAGACCGCACCAGCGACACA 5053 0.10234064995730573 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 4947 0.10019378494731675 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3053060 3.0399396 6.5096707 2
GGGGG 534260 3.022524 6.691307 4
TTTTT 2746555 3.0034416 3.92895 60-64
CCCCA 685710 2.648034 6.053857 8
CCAGC 672940 2.6211603 5.7291923 5
CCTGG 649690 2.6007452 5.685692 95-96
CCTCC 618450 2.4334807 7.930451 6
TGGGG 576070 2.3460383 8.596044 3
GGCAG 585600 2.3205268 6.5686994 8
GCTGG 561675 2.2678318 18.631365 7
GGTGG 542475 2.209223 5.57591 7
GAGGG 534665 2.1369846 8.707169 9
CACCA 735900 2.025063 5.936542 8
TGCAG 707175 2.0172286 7.8353367 3
TGGTG 679025 1.99062 12.926624 9
CAGTG 688845 1.9649419 5.4575834 5
GAGGA 695715 1.9645065 6.2055936 1
CACAC 706745 1.9448338 5.1054263 8
TGGCT 667875 1.9411705 6.784363 3
CCCAC 495190 1.9122953 5.947194 9
AAGAA 1337770 1.8854221 5.540132 2
TGTGT 893035 1.8845767 14.045285 1
GCCCC 334790 1.8300084 7.3400817 9
TCCTC 631675 1.8046538 5.1891565 5
AGGCA 644540 1.8044211 5.0367146 7
TGTTT 1158790 1.760325 6.015032 3
AGGAA 854180 1.7040173 5.023876 2
GTGGC 421895 1.7034528 5.673482 2
AGGGG 419465 1.6765455 5.223305 6
GGCTC 416490 1.6672324 8.203464 4
CTGGT 546400 1.5881048 12.014537 8
GAAAG 792200 1.5803726 6.9832406 5
ATGGA 759225 1.5432467 10.982997 2
AGCAA 755120 1.4935043 5.7643356 8
AAGCA 747275 1.4779882 5.4489965 7
CATGG 515995 1.4718845 42.63125 1
AAAGC 743470 1.4704624 5.1410394 6
TGCCC 365900 1.4521782 7.4222317 3
GCAGT 499885 1.4259304 6.7066436 4
AAGGC 490590 1.3734306 5.277563 6
TAAAA 1344460 1.3640106 8.6880455 1
ATGTG 655790 1.3582193 6.5000515 2
TGGGT 426275 1.2496618 6.7260666 3
ATGAA 853795 1.226086 8.456117 2
ATGGC 428385 1.2219754 8.323149 2
GTGTC 419405 1.2189955 15.137434 2
ATGCC 426940 1.2074271 8.20864 2
ATGGG 406825 1.170496 13.692577 2
TGACT 568330 1.1670021 5.2395086 3
CATGT 566425 1.1630903 37.446198 1
TGGTT 548620 1.1577559 5.1221447 9
AACAC 584235 1.1456288 6.0005684 2
GTCTT 545520 1.1413581 5.0113072 9
CATGA 556170 1.1208264 31.905357 1
GCAAG 397335 1.1123586 5.6311727 9
ATGCT 515660 1.0588498 7.8537583 2
ATGGT 510620 1.0575548 7.36007 2
ATGTT 698510 1.0414069 9.355375 2
TGTAG 502170 1.040054 9.465313 3
ATGAC 513380 1.0345932 9.443208 2
TAAAG 700905 1.0065295 6.781174 1
ATGAT 683430 1.0000038 6.170832 2
GTAGG 333020 0.95814824 8.138547 4
ATGTA 654535 0.9577242 10.838286 2
CATGC 337415 0.954242 32.523308 1
CGCTG 210270 0.84172255 15.801856 6
ATGCA 397230 0.800521 10.143673 2
TAAGA 537520 0.77190167 5.2168517 1
TAATG 517795 0.7576445 5.425435 1
TAAGT 513855 0.7518795 6.5879803 1
TAACT 507845 0.7367238 6.4313827 1
TAAGG 347225 0.7057906 5.8060102 1
TAAAC 475410 0.6768642 5.645387 1
TAACA 471110 0.6707421 5.0131354 1
TAGGT 320480 0.66375226 5.0775867 5
TGCCG 130925 0.52410007 7.5493355 5
TCGCT 161070 0.46414006 11.160093 5
GCCGT 106915 0.4279867 6.2301493 6
GTCGC 100645 0.4028875 15.458517 4
TGTCG 136575 0.39695355 11.373745 3
CCGTC 99675 0.3955886 6.208882 7
CGTCT 133135 0.38364235 5.0173426 8
Produced by FastQC (version 0.10.0)