##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-46.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3956860	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.853675641796777	24.0	24.0	31.0	23.0	31.0
2	30.951523219325775	31.0	31.0	32.0	27.0	33.0
3	32.55756423951229	33.0	33.0	34.0	29.0	35.0
4	33.75514542033997	34.0	33.0	36.0	29.0	37.0
5	35.31100141348012	36.0	35.0	37.0	33.0	37.0
6	35.6396987755073	36.0	36.0	38.0	33.0	38.0
7	35.65527191377001	37.0	35.0	37.0	32.0	37.0
8	35.50218799332152	36.0	36.0	37.0	32.0	38.0
9	35.507228909231905	37.0	36.0	37.0	31.0	38.0
10-14	35.86982163752563	37.0	36.0	37.4	31.8	38.0
15-19	36.84611422710665	38.0	37.0	38.4	32.6	39.4
20-24	36.47616056564031	37.4	36.0	38.8	32.8	39.8
25-29	36.1251444020326	37.6	36.2	38.0	32.6	38.2
30-34	35.935718571750336	37.6	35.4	38.2	31.6	38.6
35-39	35.939097166114806	37.8	36.4	38.0	31.2	38.6
40-44	35.88756797708034	37.4	35.8	38.4	31.6	38.8
45-49	35.84272460937075	37.6	35.6	38.2	31.4	38.6
50-54	36.04431592523745	37.6	36.2	38.2	33.0	39.0
55-59	35.75952989677055	37.2	35.6	38.2	31.4	38.6
60-64	35.787109710285826	37.4	35.6	38.2	32.4	38.6
65-69	35.58441562581074	37.4	35.8	38.2	31.4	38.6
70-74	35.61620889773826	37.2	36.0	38.4	31.4	39.0
75-79	35.324908338000526	37.4	35.4	37.6	31.0	38.4
80-84	35.42946836271908	37.0	35.6	38.2	31.0	38.4
85-89	35.12452442543946	37.2	35.2	37.6	30.4	38.2
90-94	35.14713329971048	37.0	35.0	38.2	30.8	38.4
95-99	34.96529924232457	37.4	35.0	37.6	29.4	38.2
100	33.87755621944565	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	7.0
2	44.0
3	189.0
4	0.0
5	18.0
6	39.0
7	155.0
8	236.0
9	13816.0
10	591.0
11	968.0
12	1431.0
13	2926.0
14	3921.0
15	4504.0
16	5062.0
17	5610.0
18	6692.0
19	8183.0
20	9752.0
21	9581.0
22	8857.0
23	9659.0
24	11634.0
25	15421.0
26	20357.0
27	28126.0
28	37959.0
29	49970.0
30	65044.0
31	84807.0
32	109050.0
33	145730.0
34	215576.0
35	385358.0
36	1127157.0
37	1568426.0
38	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.934733304460103	19.084292328017156	21.273837224495967	39.70713714302677
2	17.808136124995688	52.17499944191106	14.904247158312584	15.112617274780671
3	19.28388240755538	34.08818672328638	30.68709745338133	15.940833415776911
4	34.182305562010804	24.710173459560774	20.94034809628817	20.167172882140253
5	20.680905220312276	27.222806865249005	26.333506441818542	25.762781472620176
6	20.46523912071838	29.267654707244727	25.495281581406687	24.771824590630207
7	22.13616830059545	27.919061305116504	27.16112201752504	22.783648376763
8	21.634960587573413	27.38363656743219	28.663749336306072	22.317653508688323
9	21.89275283416281	28.29183660968476	26.837945337124502	22.977465219027927
10-14	22.084350784048134	27.32022608566132	26.741565951013417	23.853857179277124
15-19	21.670168095003977	27.150525549883252	27.125709074835708	24.053597280277067
20-24	21.92740790285357	26.686563566500894	27.362801578360628	24.023226952284908
25-29	21.88339459550938	27.141271389944116	27.972255734434558	23.003078280111954
30-34	22.533584758637097	27.412175031639908	27.307801191267256	22.746439018455735
35-39	22.489858089033017	26.30632000783042	28.436944686861516	22.76687721627505
40-44	22.68156368839895	27.38228561315722	27.170352940135743	22.765797758308086
45-49	22.292475691292935	27.815812410452782	27.794277318240063	22.09743458001422
50-54	22.706498806166774	27.35416243310106	27.4458166002594	22.49352216047277
55-59	22.09097055923516	28.156909203251836	27.234259082330624	22.51786115518238
60-64	22.56506578586771	27.6304690813595	27.9631860425043	21.841279090268493
65-69	22.563231820713064	28.651746979447562	27.548623102454528	21.236398097384843
70-74	22.222762304853095	28.192164587437073	27.24117469565901	22.34389841205082
75-79	22.829503372031436	28.152896467634996	27.32036677805651	21.697233382277055
80-84	21.986629618267234	28.058272606431206	27.519920124964255	22.43517765033731
85-89	22.298136067847988	27.893449948775473	27.799864801010077	22.008549182366465
90-94	22.358248552022854	27.55946222914274	27.680086519127244	22.402202699707164
95-99	22.556055798886256	26.793804509504447	28.02196604360206	22.628173648007238
100	23.656190618171784	26.993310296336183	26.429669664591408	22.920829420900624
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	59.64187044748688
2	32.92075339977636
3	35.22471582333229
4	54.34947844415105
5	46.44368669293245
6	45.23706371134858
7	44.919816677358455
8	43.95261409626173
9	44.87021805319073
10-14	45.93820796332526
15-19	45.72376537528104
20-24	45.950634855138475
25-29	44.88647287562133
30-34	45.280023777092836
35-39	45.25673530530806
40-44	45.447361446707035
45-49	44.38991027130715
50-54	45.20002096663955
55-59	44.60883171441754
60-64	44.4063448761362
65-69	43.79962991809791
70-74	44.56666071690391
75-79	44.526736754308494
80-84	44.42180726860454
85-89	44.30668525021446
90-94	44.76045125173002
95-99	45.18422944689349
100	46.57702003907241
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14342.0
1	14343.5
2	14341.0
3	14337.5
4	14339.0
5	14341.5
6	14347.5
7	14357.0
8	14372.5
9	14385.0
10	14414.0
11	14463.0
12	14492.0
13	14531.0
14	14553.0
15	14798.5
16	14976.0
17	14920.0
18	15157.5
19	15762.5
20	16662.5
21	17877.5
22	20611.5
23	25512.5
24	35992.0
25	45068.0
26	52853.5
27	63988.5
28	66177.5
29	74181.0
30	91849.5
31	102253.5
32	108025.0
33	118642.5
34	130416.5
35	131682.5
36	127197.5
37	120928.0
38	135799.0
39	149114.0
40	153410.0
41	164986.0
42	160613.0
43	160522.5
44	165497.0
45	157034.0
46	143043.0
47	127300.0
48	122391.0
49	134132.5
50	136062.5
51	133857.5
52	114872.0
53	77127.5
54	66218.0
55	65503.0
56	53146.0
57	44912.5
58	58595.5
59	58717.0
60	57598.5
61	73601.5
62	68515.5
63	62340.5
64	57596.0
65	51426.0
66	51839.5
67	46970.5
68	36111.0
69	31231.0
70	30113.0
71	26546.5
72	29725.5
73	21231.5
74	9981.0
75	12771.5
76	13002.5
77	12165.0
78	10556.5
79	10021.5
80	9056.0
81	7664.0
82	6629.5
83	6581.0
84	7531.5
85	7332.5
86	7060.5
87	5798.0
88	5011.5
89	5236.5
90	5461.5
91	5753.5
92	4809.0
93	3862.5
94	3843.5
95	3842.5
96	3835.0
97	3826.5
98	3827.5
99	3836.0
100	3832.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05228893617666533
2	0.008040907673892239
3	0.0018010426261129365
4	0.007204552465377559
5	0.004949337816671959
6	8.634275942316942E-4
7	4.320751221057003E-4
8	2.033332935138967E-4
9	1.0166706020291221E-4
10-14	0.0014030392371614665
15-19	0.018324218678500464
20-24	0.016603548652077006
25-29	0.008358479279817017
30-34	0.008002055588981576
35-39	0.005245695857739606
40-44	0.012707042252072543
45-49	0.01164396522418082
50-54	0.010544178222017121
55-59	0.010512877572781
60-64	0.0548595417156262
65-69	0.044452098618905464
70-74	0.027738384810262917
75-79	0.07367508803728097
80-84	0.06486068120625142
85-89	0.014808871146859689
90-94	0.00633225212976917
95-99	0.009048919568337983
100	0.007397844390514962
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	14519.0
2-3	389.0
4-5	4158.0
6-7	3367.0
8-9	31.0
10-11	65.0
12-13	109.0
14-15	259.0
16-17	141.0
18-19	2464.0
20-21	25080.0
22-23	2274.0
24-25	3141.0
26-27	1300.0
28-29	1213.0
30-31	2252.0
32-33	1167.0
34-35	1541.0
36-37	1300.0
38-39	1038.0
40-41	2232.0
42-43	1360.0
44-45	1228.0
46-47	1996.0
48-49	908.0
50-51	12625.0
52-53	1882.0
54-55	16230.0
56-57	10957.0
58-59	30897.0
60-61	1342.0
62-63	8738.0
64-65	4780.0
66-67	5898.0
68-69	6884.0
70-71	17484.0
72-73	17332.0
74-75	12300.0
76-77	11349.0
78-79	4181.0
80-81	7463.0
82-83	8139.0
84-85	6910.0
86-87	2156.0
88-89	3632.0
90-91	8515.0
92-93	2480.0
94-95	2822.0
96-97	82690.0
98-99	0.0
100-101	3595642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	96.65446252362673
#Duplication Level	Relative count
1	100.0
2	6.228376316306335
3	2.5744459049086705
4	2.007886506821375
5	1.906661227696445
6	1.7291392829624257
7	1.6724833431536963
8	1.4594570094728732
9	1.1935517986372357
10++	9.6949644200698
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	13424	0.33925890731539654	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10293	0.2601305075236425	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10174	0.25712307233513443	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	8351	0.2110511870523597	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	8290	0.20950956061119172	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	8006	0.20233215226214724	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	7910	0.19990598605965337	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	7657	0.19351202721349758	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	7608	0.1922736715476413	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	7375	0.18638516399367175	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	7361	0.18603134808914137	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	7356	0.18590498526609484	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	7143	0.1805219290043115	No Hit
TGAGACCGCACCAGCGACACA	6837	0.1727885242338622	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	6805	0.17197980216636424	No Hit
TGTGTCGCTGGTGCGGTCTCA	6734	0.17018545007910313	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	6722	0.16988217930379138	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	6655	0.16818891747496753	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	6621	0.16732965027825092	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	6379	0.1612136896427976	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	6345	0.160354422446081	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	6039	0.1526210176756317	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	5978	0.1510793912344637	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	5911	0.14938612940563983	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	5899	0.14908285863032808	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	5713	0.14438216161299616	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	5709	0.14428107135455892	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	5660	0.14304271568870267	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	5517	0.1394287389495711	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	5498	0.1389485602219942	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	5368	0.13566312682278373	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	5363	0.13553676399973716	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	5347	0.1351324029659882	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	5337	0.13487967731989506	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	5328	0.13465222423841128	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	5310	0.13419731807544366	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	5284	0.13354023139560156	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	5139	0.12987570952725142	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	5111	0.1291680777181907	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	5038	0.12732318050171096	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	4769	0.12052486062180617	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	4538	0.11468689819705526	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	4490	0.1134738150958083	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	4392	0.11099710376409579	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	4381	0.11071910555339336	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	4290	0.108419302173946	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	4280	0.1081665765278529	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	4256	0.10756003497722942	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	4148	0.10483059799942379	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	4048	0.10230334153849263	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	4036	0.1020000707631809	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2274995	3.7627869	6.713652	2
TTTTT	1951540	3.529806	4.5279307	95-96
GGGGG	630840	3.116841	8.36157	4
GGAGG	685515	2.7211921	5.0916886	4
CCAGG	692840	2.6574898	5.354592	5
CCAGC	698660	2.6342247	8.018393	5
CCTCC	683350	2.5783815	9.979873	6
CCCCA	693070	2.5686934	5.320502	1
CAGCC	679700	2.5627382	6.306532	6
CCTGG	656135	2.5621274	5.5953426	9
TGGGG	591580	2.390697	10.421783	3
CTCCC	613670	2.3154688	5.549218	3
GCTGG	551395	2.1903934	7.2579923	7
GGGGA	531665	2.110475	5.867348	7
GAGGG	528785	2.099043	6.42704	9
CTCCT	648720	2.0367076	5.5432873	4
CTCCA	650330	2.0055633	6.1309614	6
AAGAA	967025	1.9907655	5.043896	2
CCCTC	523375	1.9747721	6.298983	5
TCCAG	627650	1.9691185	6.6410627	7
TGGCT	591880	1.9231296	7.6561036	3
GCCAG	499130	1.9144866	7.287714	4
CCCAC	512170	1.8982321	5.6444893	9
TGTTT	848500	1.876332	5.574865	3
GGCTC	475160	1.8554422	7.923952	4
TCCTC	589355	1.8503265	6.2520423	5
CACCA	608410	1.8430198	5.297291	8
TGCAG	577010	1.8415748	5.389347	3
TGGTG	555430	1.8359294	5.251497	9
GCCCA	474265	1.7881666	5.9885454	4
AGGGG	436415	1.7323747	7.626812	6
GGCCC	350945	1.6469456	5.2249513	1
GCTCC	412255	1.5824198	8.218048	2
TAAAA	927660	1.5620209	8.455153	1
TGCCC	397630	1.5262825	12.080344	3
GATGG	463130	1.5036981	6.3367224	1
ATGGA	559755	1.4601669	10.347681	2
CATGG	445785	1.4227594	42.550423	1
GGGGT	332560	1.3439437	5.2646723	4
TGCTC	405000	1.2935349	5.9356484	1
TGGGT	386415	1.2772639	6.0812793	3
ATGTG	476050	1.2642298	7.968939	2
ATGAA	598025	1.253345	9.685535	2
TGAGA	477775	1.2463154	5.998399	1
CTCAC	400875	1.236265	5.1007414	7
ATGGC	386655	1.2340412	11.96391	2
AGACA	485070	1.221767	5.2974615	2
ATGGG	374950	1.217394	14.909428	2
ATGCC	387615	1.2160597	12.551721	2
GTCTT	424420	1.1279409	5.670626	9
GAGAC	357075	1.1194292	6.402098	2
CATGT	411685	1.0746984	32.39091	1
CATGA	415235	1.0647477	29.94444	1
ATGTT	487385	1.0586714	7.9358687	2
TAAAG	491300	1.0296699	6.54394	1
ATGCT	381720	0.99647534	8.067058	2
AGACT	388295	0.9956681	5.1204557	1
ATGAT	461005	0.9836164	6.85012	2
CACTC	316260	0.97531927	5.6277018	1
ATGGT	367235	0.97525346	8.292105	2
AGACC	314970	0.9706321	5.7271333	3
ATGAC	373865	0.9586666	7.091939	2
GCGCC	195215	0.91612214	5.3863664	8
ATGTA	425980	0.90888584	9.315208	2
CATGC	286890	0.90005624	32.13309	1
CGCTG	217600	0.8497015	5.42923	6
TAACT	355510	0.74562454	5.3909354	1
TAAGT	348125	0.7427717	6.2432513	1
ATGCA	285800	0.7328499	8.673637	2
TAAAC	333815	0.68771	5.2319627	1
TAAGG	248195	0.64743716	5.0436664	1
TGCCG	152900	0.597056	9.178925	5
CCGTC	125660	0.4823395	6.5009513	7
GCCGT	119630	0.46714061	6.6462526	6
CGTCT	144590	0.4618079	5.9116116	8
>>END_MODULE