FastQCFastQC Report
Wed 20 Mar 2013
8016-8-46.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-46.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3956860
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 13424 0.33925890731539654 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10293 0.2601305075236425 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10174 0.25712307233513443 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8351 0.2110511870523597 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8290 0.20950956061119172 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 8006 0.20233215226214724 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 7910 0.19990598605965337 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 7657 0.19351202721349758 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 7608 0.1922736715476413 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 7375 0.18638516399367175 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 7361 0.18603134808914137 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7356 0.18590498526609484 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 7143 0.1805219290043115 No Hit
TGAGACCGCACCAGCGACACA 6837 0.1727885242338622 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 6805 0.17197980216636424 No Hit
TGTGTCGCTGGTGCGGTCTCA 6734 0.17018545007910313 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 6722 0.16988217930379138 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 6655 0.16818891747496753 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 6621 0.16732965027825092 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 6379 0.1612136896427976 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6345 0.160354422446081 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6039 0.1526210176756317 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 5978 0.1510793912344637 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 5911 0.14938612940563983 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 5899 0.14908285863032808 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5713 0.14438216161299616 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 5709 0.14428107135455892 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 5660 0.14304271568870267 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 5517 0.1394287389495711 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 5498 0.1389485602219942 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 5368 0.13566312682278373 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5363 0.13553676399973716 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 5347 0.1351324029659882 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5337 0.13487967731989506 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 5328 0.13465222423841128 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 5310 0.13419731807544366 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 5284 0.13354023139560156 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5139 0.12987570952725142 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5111 0.1291680777181907 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 5038 0.12732318050171096 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 4769 0.12052486062180617 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 4538 0.11468689819705526 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 4490 0.1134738150958083 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 4392 0.11099710376409579 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 4381 0.11071910555339336 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 4290 0.108419302173946 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 4280 0.1081665765278529 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 4256 0.10756003497722942 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 4148 0.10483059799942379 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 4048 0.10230334153849263 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 4036 0.1020000707631809 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2274995 3.7627869 6.713652 2
TTTTT 1951540 3.529806 4.5279307 95-96
GGGGG 630840 3.116841 8.36157 4
GGAGG 685515 2.7211921 5.0916886 4
CCAGG 692840 2.6574898 5.354592 5
CCAGC 698660 2.6342247 8.018393 5
CCTCC 683350 2.5783815 9.979873 6
CCCCA 693070 2.5686934 5.320502 1
CAGCC 679700 2.5627382 6.306532 6
CCTGG 656135 2.5621274 5.5953426 9
TGGGG 591580 2.390697 10.421783 3
CTCCC 613670 2.3154688 5.549218 3
GCTGG 551395 2.1903934 7.2579923 7
GGGGA 531665 2.110475 5.867348 7
GAGGG 528785 2.099043 6.42704 9
CTCCT 648720 2.0367076 5.5432873 4
CTCCA 650330 2.0055633 6.1309614 6
AAGAA 967025 1.9907655 5.043896 2
CCCTC 523375 1.9747721 6.298983 5
TCCAG 627650 1.9691185 6.6410627 7
TGGCT 591880 1.9231296 7.6561036 3
GCCAG 499130 1.9144866 7.287714 4
CCCAC 512170 1.8982321 5.6444893 9
TGTTT 848500 1.876332 5.574865 3
GGCTC 475160 1.8554422 7.923952 4
TCCTC 589355 1.8503265 6.2520423 5
CACCA 608410 1.8430198 5.297291 8
TGCAG 577010 1.8415748 5.389347 3
TGGTG 555430 1.8359294 5.251497 9
GCCCA 474265 1.7881666 5.9885454 4
AGGGG 436415 1.7323747 7.626812 6
GGCCC 350945 1.6469456 5.2249513 1
GCTCC 412255 1.5824198 8.218048 2
TAAAA 927660 1.5620209 8.455153 1
TGCCC 397630 1.5262825 12.080344 3
GATGG 463130 1.5036981 6.3367224 1
ATGGA 559755 1.4601669 10.347681 2
CATGG 445785 1.4227594 42.550423 1
GGGGT 332560 1.3439437 5.2646723 4
TGCTC 405000 1.2935349 5.9356484 1
TGGGT 386415 1.2772639 6.0812793 3
ATGTG 476050 1.2642298 7.968939 2
ATGAA 598025 1.253345 9.685535 2
TGAGA 477775 1.2463154 5.998399 1
CTCAC 400875 1.236265 5.1007414 7
ATGGC 386655 1.2340412 11.96391 2
AGACA 485070 1.221767 5.2974615 2
ATGGG 374950 1.217394 14.909428 2
ATGCC 387615 1.2160597 12.551721 2
GTCTT 424420 1.1279409 5.670626 9
GAGAC 357075 1.1194292 6.402098 2
CATGT 411685 1.0746984 32.39091 1
CATGA 415235 1.0647477 29.94444 1
ATGTT 487385 1.0586714 7.9358687 2
TAAAG 491300 1.0296699 6.54394 1
ATGCT 381720 0.99647534 8.067058 2
AGACT 388295 0.9956681 5.1204557 1
ATGAT 461005 0.9836164 6.85012 2
CACTC 316260 0.97531927 5.6277018 1
ATGGT 367235 0.97525346 8.292105 2
AGACC 314970 0.9706321 5.7271333 3
ATGAC 373865 0.9586666 7.091939 2
GCGCC 195215 0.91612214 5.3863664 8
ATGTA 425980 0.90888584 9.315208 2
CATGC 286890 0.90005624 32.13309 1
CGCTG 217600 0.8497015 5.42923 6
TAACT 355510 0.74562454 5.3909354 1
TAAGT 348125 0.7427717 6.2432513 1
ATGCA 285800 0.7328499 8.673637 2
TAAAC 333815 0.68771 5.2319627 1
TAAGG 248195 0.64743716 5.0436664 1
TGCCG 152900 0.597056 9.178925 5
CCGTC 125660 0.4823395 6.5009513 7
GCCGT 119630 0.46714061 6.6462526 6
CGTCT 144590 0.4618079 5.9116116 8
Produced by FastQC (version 0.10.0)