##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-47.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4492544	
Filtered Sequences	0	
Sequence length	1-100	
%GC	42	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.90846255484643	24.0	24.0	31.0	23.0	31.0
2	30.986382771223937	31.0	31.0	32.0	29.0	33.0
3	32.60384522382454	33.0	33.0	34.0	29.0	35.0
4	33.81344081751812	34.0	33.0	36.0	29.0	37.0
5	35.33902534263285	36.0	35.0	37.0	33.0	37.0
6	35.693057301907594	36.0	36.0	38.0	33.0	38.0
7	35.686904645630484	37.0	35.0	37.0	34.0	37.0
8	35.55963330974867	36.0	36.0	37.0	32.0	38.0
9	35.56576712557618	37.0	36.0	37.0	31.0	38.0
10-14	35.930617367487955	37.0	36.0	37.4	32.2	38.0
15-19	36.91427089163286	38.0	37.2	38.4	32.6	39.4
20-24	36.58013651724295	37.4	36.0	38.8	32.8	39.8
25-29	36.23035146796453	37.6	36.2	38.0	32.8	38.2
30-34	36.063913893726365	37.6	35.6	38.2	32.4	38.6
35-39	36.086112032852064	37.8	36.4	38.0	31.6	38.6
40-44	36.03981736111396	37.4	36.0	38.4	32.4	38.8
45-49	35.974824468886844	37.6	35.8	38.2	32.0	38.6
50-54	36.18706161861793	37.6	36.2	38.4	33.0	39.0
55-59	35.91449553235991	37.6	35.8	38.2	32.4	38.6
60-64	35.968553370134124	37.6	35.6	38.2	32.6	38.6
65-69	35.77922439668662	37.4	36.0	38.2	31.6	38.6
70-74	35.805360900107324	37.4	36.0	38.4	31.8	39.0
75-79	35.51005985250183	37.4	35.4	37.6	31.6	38.4
80-84	35.623067565528004	37.2	35.8	38.2	31.2	38.6
85-89	35.34343985802845	37.2	35.4	37.6	31.0	38.2
90-94	35.355486607967165	37.0	35.0	38.2	30.8	38.4
95-99	35.20333186504223	37.4	35.2	37.6	31.0	38.2
100	34.12893316724876	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	24.0
3	103.0
4	0.0
5	11.0
6	38.0
7	123.0
8	240.0
9	21709.0
10	763.0
11	1208.0
12	1887.0
13	3195.0
14	4270.0
15	5013.0
16	5596.0
17	6351.0
18	7324.0
19	8340.0
20	8959.0
21	8965.0
22	8899.0
23	9685.0
24	11931.0
25	15235.0
26	20558.0
27	27968.0
28	38554.0
29	50951.0
30	67284.0
31	88334.0
32	113514.0
33	153191.0
34	224815.0
35	407340.0
36	1260518.0
37	1909641.0
38	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.678011355728234	20.637650947630913	22.654753907755182	38.029583788885674
2	16.794492508850126	53.968849791120235	15.086074122956642	14.150583577072998
3	18.556312453290403	36.52934911790582	30.221761119316863	14.692577309486918
4	31.93550292363121	26.097860657248763	22.211140404100064	19.755496015019965
5	20.00662255809973	28.30209349904963	27.740206634566526	23.951077308284113
6	19.917221328631328	30.233552506129886	27.14164574766638	22.70758041757241
7	20.756205237039087	29.042188831044847	28.883176334126524	21.318429597789542
8	20.200141283596956	28.84293853714443	29.71141051417619	21.24550966508243
9	20.914373427905623	29.512194355212827	28.475560766991194	21.097871449890356
10-14	21.221376904961584	28.564815243123796	27.764504357668812	22.449303494245807
15-19	20.716360620604974	28.41795108863179	28.364890267148567	22.50079802361467
20-24	21.04135075531888	27.72339372077935	28.592606298868763	22.64264922503301
25-29	20.979967546686538	28.130475728638686	29.134361759483163	21.755194965191613
30-34	21.76467549966721	28.35013620009954	28.372777420073238	21.51241088016001
35-39	21.62440503612513	27.290967189121822	29.456265556784317	21.62836221796873
40-44	21.66530528270601	28.30550407724111	28.227799830561512	21.801390809491362
45-49	21.500817355615254	28.913404768202668	28.61754608202412	20.96823179415796
50-54	21.591010366121044	28.381192433007474	28.52452985770764	21.50326734316384
55-59	21.170175189647548	29.24852867738329	28.25508035682417	21.326215776144988
60-64	21.424164505410037	28.892723248064804	28.92768545476984	20.755426791755315
65-69	21.326637900533253	29.73021581649446	28.649011571706566	20.29413471126572
70-74	21.295862747334233	29.2005033339993	28.236642263075147	21.26699165559132
75-79	21.766228133754307	29.162676736070598	28.291432874765327	20.779662255409768
80-84	21.04490919753225	29.103611041884708	28.68021540289247	21.17126435769058
85-89	21.285552818291613	28.77139328773114	28.708617828201987	21.23443606577526
90-94	21.051132169412586	28.62049651562674	28.90583214419668	21.422539170763994
95-99	21.212633196626456	28.066237066079104	29.035316778840368	21.685812958454072
100	22.358394367663326	28.234966103985247	27.699041801948944	21.707597726402483
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	56.70759514461391
2	30.945076085923123
3	33.24888976277732
4	51.69099893865118
5	43.95769986638384
6	42.62480174620373
7	42.074634834828636
8	41.445650948679386
9	42.01224487779597
10-14	43.67068039920739
15-19	43.217158644219644
20-24	43.68399998035189
25-29	42.73516251187815
30-34	43.277086379827225
35-39	43.25276725409386
40-44	43.466696092197374
45-49	42.46904914977321
50-54	43.09427770928488
55-59	42.496390965792536
60-64	42.17959129716535
65-69	41.62077261179897
70-74	42.56285440292555
75-79	42.54589038916408
80-84	42.216173555222824
85-89	42.519988884066876
90-94	42.47367134017658
95-99	42.89844615508053
100	44.065992094065805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21726.0
1	21722.5
2	21720.0
3	21723.0
4	21726.0
5	21733.0
6	21745.0
7	21755.5
8	21774.0
9	21793.0
10	21822.5
11	21853.5
12	21866.0
13	21896.0
14	21940.0
15	22441.5
16	22768.5
17	22741.0
18	22997.0
19	23595.0
20	25222.5
21	26904.5
22	30526.0
23	35837.0
24	47841.0
25	60109.5
26	73862.5
27	91210.0
28	92475.5
29	103387.5
30	127690.5
31	142197.0
32	152455.0
33	165868.0
34	179165.0
35	179237.0
36	170274.0
37	158836.0
38	177564.5
39	196643.0
40	198434.5
41	207279.5
42	199799.5
43	200094.0
44	203130.0
45	187964.5
46	166627.0
47	144638.0
48	140385.0
49	150010.5
50	146387.5
51	139248.5
52	116311.0
53	77446.5
54	65405.5
55	62368.5
56	48697.5
57	42118.5
58	53525.0
59	53765.5
60	51411.0
61	59463.5
62	53417.5
63	46886.0
64	42084.5
65	38561.5
66	39637.0
67	37291.0
68	29023.0
69	24842.0
70	23912.5
71	22241.0
72	24335.0
73	17614.5
74	9753.5
75	11981.0
76	13437.5
77	12616.5
78	11114.5
79	10885.5
80	9959.0
81	8663.5
82	8186.5
83	8161.5
84	8069.0
85	7367.5
86	8621.5
87	8506.5
88	8270.0
89	9397.0
90	10923.0
91	12514.0
92	11379.0
93	10217.0
94	10186.5
95	10190.0
96	10184.5
97	10177.0
98	10174.0
99	10170.5
100	10169.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05217533762607556
2	0.00861170386475375
3	0.002057920162987277
4	0.00740473119819065
5	0.004726974648854112
6	7.851573847977827E-4
7	3.8170038540512443E-4
8	1.1227228093937769E-4
9	4.4908942627927616E-5
10-14	0.00141015625070171
15-19	0.01859388379792742
20-24	0.017031469785654448
25-29	0.008311867784203314
30-34	0.0077560288878835955
35-39	0.005383107473718076
40-44	0.013303161979454317
45-49	0.011840386150831095
50-54	0.010590672334747168
55-59	0.011041117350080525
60-64	0.054886037672952116
65-69	0.04435434915764054
70-74	0.02791524913554471
75-79	0.07486574287185611
80-84	0.06517397902619
85-89	0.014793390087107549
90-94	0.006745383449563372
95-99	0.009281049413319555
100	0.007940979706765276
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	21884.0
2-3	545.0
4-5	12410.0
6-7	4246.0
8-9	10.0
10-11	32.0
12-13	52.0
14-15	82.0
16-17	163.0
18-19	1206.0
20-21	17527.0
22-23	2456.0
24-25	3699.0
26-27	1634.0
28-29	2021.0
30-31	2677.0
32-33	1490.0
34-35	2006.0
36-37	1700.0
38-39	953.0
40-41	2068.0
42-43	1185.0
44-45	2239.0
46-47	4027.0
48-49	1110.0
50-51	13438.0
52-53	1678.0
54-55	17881.0
56-57	10038.0
58-59	29573.0
60-61	1960.0
62-63	8272.0
64-65	4482.0
66-67	7197.0
68-69	7244.0
70-71	20662.0
72-73	18669.0
74-75	12185.0
76-77	13344.0
78-79	5120.0
80-81	8336.0
82-83	10294.0
84-85	4538.0
86-87	2153.0
88-89	3669.0
90-91	8131.0
92-93	2335.0
94-95	2886.0
96-97	98343.0
98-99	0.0
100-101	4092694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	96.82979035031838
#Duplication Level	Relative count
1	100.0
2	5.433111225012814
3	1.5257367138734614
4	0.6965167142947417
5	0.5174725992290571
6	0.40302479234393324
7	0.34755622336279957
8	0.35317329363937006
9	0.3335135476713733
10++	10.75037037557136
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	21266	0.47336208615875547	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	10094	0.22468338651775033	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	10058	0.2238820588067696	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	8126	0.1808774716508063	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	8124	0.18083295344464073	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	7849	0.17471170009687162	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	7694	0.17126153911903813	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	7206	0.1603990968146333	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	7075	0.15748315431078694	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	6928	0.15421106615761582	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	6399	0.14243600062681636	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	6273	0.13963135363838397	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	6022	0.134044318764602	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	5985	0.1332207319505385	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	5982	0.13315395464129012	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	5969	0.13286458630121376	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	5942	0.13226359051797823	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	5812	0.12936990711721466	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	5770	0.1284350247877372	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	5723	0.12738884694284575	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	5691	0.12667655564419625	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	5676	0.1263426690979543	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	5594	0.12451742264516497	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	5565	0.12387190865576385	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	5553	0.1236047994187703	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	5548	0.12349350390335632	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	5529	0.12307058094478318	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	5447	0.12124533449199383	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	5312	0.11824035557581629	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	5145	0.11452308536098924	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	5112	0.11378853495925695	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	5036	0.11209684312496439	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	5018	0.11169617926947405	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	4883	0.10869120035329649	No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA	4838	0.10768954071457063	No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC	4712	0.10488489372613824	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	4684	0.10426163883981994	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	4652	0.10354934754117043	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	4649	0.10348257023192205	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	4600	0.10239187418086501	No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA	4581	0.10196895122229187	No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG	4580	0.10194669211920906	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	4577	0.10187991480996067	No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG	4550	0.10127891902672517	No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG	4533	0.10090051427431763	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	646895	3.5533283	7.156127	40-44
AAAAA	2836710	3.3971188	6.301061	2
TTTTT	2405780	3.1635144	3.9061258	95-96
CCTGG	662430	2.671551	5.4999075	9
CCAGC	668675	2.6164632	8.270805	5
CCCCA	668920	2.5874588	5.4610124	1
CCTCC	648065	2.554121	8.363421	6
TGGGG	589805	2.4340663	8.6204	3
GCTGG	541830	2.210481	6.346451	7
GAGGG	528050	2.1388261	7.025546	9
CACAC	687620	1.9840237	5.008572	8
GCCAG	497810	1.9704409	6.554498	4
CCCAC	508825	1.968193	6.533671	9
TCCAG	650035	1.9331208	5.315729	4
TGGCT	626340	1.9198015	5.639137	3
CACCA	649850	1.8750441	5.4516172	8
TGCAG	611685	1.840138	5.8402376	3
TGTTT	1046850	1.8322128	5.6559196	3
TCCTC	610110	1.8274847	5.4885416	5
GCCCA	460130	1.8004459	5.858448	4
AGGGG	442240	1.7912592	5.904653	6
GGCTC	437140	1.762966	6.5117035	1
GGCCC	304150	1.613944	6.0334525	1
TGCCC	391940	1.5625817	10.158636	3
GCTCC	383310	1.5281758	6.683365	2
GATGG	491270	1.4950067	5.081349	1
ATGGA	664810	1.4918295	9.793859	2
CATGG	488595	1.469845	39.746807	1
TAAAA	1191105	1.4533474	8.221435	1
GCAGT	448230	1.3484147	5.3448925	4
TGCTC	434055	1.3151964	5.1840453	1
ATGTG	563950	1.2893947	7.2431984	2
TGGGT	413770	1.2829374	6.2240796	3
ATGAA	751685	1.2438169	8.04233	2
ATGGC	413305	1.2433494	9.606306	2
ATGCC	416815	1.2395544	10.428306	2
ATGGG	393390	1.1971432	13.448277	2
GTCTT	500670	1.1529759	5.6557264	9
CATGT	491805	1.1115731	31.551353	1
ATGTT	615155	1.0567015	7.9688535	2
CATGA	474920	1.0535178	27.85351	1
TAAAG	624960	1.0341244	6.7887335	1
ATGCT	455680	1.0299234	6.9773884	2
ATGGT	447760	1.0237421	7.0892005	2
ATGAC	432950	0.9604155	7.0275903	2
CATGC	316070	0.93995184	30.512579	1
ATGTA	553575	0.93329865	8.260421	2
ATGAT	553275	0.93279284	5.8158092	2
ATGCA	341020	0.7564866	8.424185	2
TAACT	441975	0.7366168	5.823639	1
TAAGT	436565	0.73602587	6.318115	1
TAAAC	425305	0.695698	5.3633113	1
TAAGG	296875	0.6661857	5.5527844	1
TGCCG	135415	0.5461226	9.0211115	5
CCGTC	112140	0.44707847	6.3606224	7
GCCGT	108455	0.43739417	6.4569125	6
CGTCT	133785	0.4053716	5.4327602	8
>>END_MODULE