FastQCFastQC Report
Wed 20 Mar 2013
8016-8-47.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-47.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4492544
Filtered Sequences 0
Sequence length 1-100
%GC 42

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 21266 0.47336208615875547 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 10094 0.22468338651775033 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10058 0.2238820588067696 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8126 0.1808774716508063 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8124 0.18083295344464073 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 7849 0.17471170009687162 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7694 0.17126153911903813 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7206 0.1603990968146333 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 7075 0.15748315431078694 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 6928 0.15421106615761582 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 6399 0.14243600062681636 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6273 0.13963135363838397 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 6022 0.134044318764602 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 5985 0.1332207319505385 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 5982 0.13315395464129012 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 5969 0.13286458630121376 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 5942 0.13226359051797823 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 5812 0.12936990711721466 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 5770 0.1284350247877372 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 5723 0.12738884694284575 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 5691 0.12667655564419625 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 5676 0.1263426690979543 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 5594 0.12451742264516497 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 5565 0.12387190865576385 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 5553 0.1236047994187703 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 5548 0.12349350390335632 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 5529 0.12307058094478318 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 5447 0.12124533449199383 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 5312 0.11824035557581629 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5145 0.11452308536098924 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5112 0.11378853495925695 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5036 0.11209684312496439 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5018 0.11169617926947405 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 4883 0.10869120035329649 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 4838 0.10768954071457063 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 4712 0.10488489372613824 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 4684 0.10426163883981994 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 4652 0.10354934754117043 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 4649 0.10348257023192205 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 4600 0.10239187418086501 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 4581 0.10196895122229187 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 4580 0.10194669211920906 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 4577 0.10187991480996067 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 4550 0.10127891902672517 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 4533 0.10090051427431763 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 646895 3.5533283 7.156127 40-44
AAAAA 2836710 3.3971188 6.301061 2
TTTTT 2405780 3.1635144 3.9061258 95-96
CCTGG 662430 2.671551 5.4999075 9
CCAGC 668675 2.6164632 8.270805 5
CCCCA 668920 2.5874588 5.4610124 1
CCTCC 648065 2.554121 8.363421 6
TGGGG 589805 2.4340663 8.6204 3
GCTGG 541830 2.210481 6.346451 7
GAGGG 528050 2.1388261 7.025546 9
CACAC 687620 1.9840237 5.008572 8
GCCAG 497810 1.9704409 6.554498 4
CCCAC 508825 1.968193 6.533671 9
TCCAG 650035 1.9331208 5.315729 4
TGGCT 626340 1.9198015 5.639137 3
CACCA 649850 1.8750441 5.4516172 8
TGCAG 611685 1.840138 5.8402376 3
TGTTT 1046850 1.8322128 5.6559196 3
TCCTC 610110 1.8274847 5.4885416 5
GCCCA 460130 1.8004459 5.858448 4
AGGGG 442240 1.7912592 5.904653 6
GGCTC 437140 1.762966 6.5117035 1
GGCCC 304150 1.613944 6.0334525 1
TGCCC 391940 1.5625817 10.158636 3
GCTCC 383310 1.5281758 6.683365 2
GATGG 491270 1.4950067 5.081349 1
ATGGA 664810 1.4918295 9.793859 2
CATGG 488595 1.469845 39.746807 1
TAAAA 1191105 1.4533474 8.221435 1
GCAGT 448230 1.3484147 5.3448925 4
TGCTC 434055 1.3151964 5.1840453 1
ATGTG 563950 1.2893947 7.2431984 2
TGGGT 413770 1.2829374 6.2240796 3
ATGAA 751685 1.2438169 8.04233 2
ATGGC 413305 1.2433494 9.606306 2
ATGCC 416815 1.2395544 10.428306 2
ATGGG 393390 1.1971432 13.448277 2
GTCTT 500670 1.1529759 5.6557264 9
CATGT 491805 1.1115731 31.551353 1
ATGTT 615155 1.0567015 7.9688535 2
CATGA 474920 1.0535178 27.85351 1
TAAAG 624960 1.0341244 6.7887335 1
ATGCT 455680 1.0299234 6.9773884 2
ATGGT 447760 1.0237421 7.0892005 2
ATGAC 432950 0.9604155 7.0275903 2
CATGC 316070 0.93995184 30.512579 1
ATGTA 553575 0.93329865 8.260421 2
ATGAT 553275 0.93279284 5.8158092 2
ATGCA 341020 0.7564866 8.424185 2
TAACT 441975 0.7366168 5.823639 1
TAAGT 436565 0.73602587 6.318115 1
TAAAC 425305 0.695698 5.3633113 1
TAAGG 296875 0.6661857 5.5527844 1
TGCCG 135415 0.5461226 9.0211115 5
CCGTC 112140 0.44707847 6.3606224 7
GCCGT 108455 0.43739417 6.4569125 6
CGTCT 133785 0.4053716 5.4327602 8
Produced by FastQC (version 0.10.0)