##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-48.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9776940	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.945115854244783	24.0	24.0	31.0	23.0	31.0
2	31.008154320860495	31.0	31.0	32.0	27.0	33.0
3	32.653733660230365	33.0	33.0	34.0	29.0	35.0
4	33.86262785524634	34.0	33.0	36.0	29.0	37.0
5	35.40525429194248	36.0	35.0	37.0	33.0	37.0
6	35.7287641251089	36.0	36.0	38.0	33.0	38.0
7	35.751350822416704	37.0	35.0	37.0	34.0	37.0
8	35.59801204388922	36.0	36.0	37.0	32.0	38.0
9	35.62179396183833	37.0	36.0	37.0	31.0	38.0
10-14	35.98768414278784	37.0	36.0	37.4	32.4	38.0
15-19	36.97674815991486	38.0	37.2	38.4	32.6	39.4
20-24	36.61803721615276	37.4	36.0	38.8	32.8	39.8
25-29	36.27677929644791	37.6	36.2	38.0	32.8	38.2
30-34	36.11016234739152	37.6	35.6	38.2	32.4	38.6
35-39	36.14435542836933	37.8	36.4	38.0	31.6	38.6
40-44	36.07128905866547	37.4	36.0	38.4	32.4	38.8
45-49	36.027379284291904	37.6	35.8	38.2	32.0	38.6
50-54	36.23901712616592	37.6	36.2	38.4	33.0	39.0
55-59	35.95965304400396	37.6	35.8	38.2	32.4	38.6
60-64	35.975510422079935	37.6	35.6	38.2	32.6	38.6
65-69	35.77933263223	37.4	36.0	38.2	31.4	38.6
70-74	35.82393816032695	37.4	36.0	38.4	31.8	39.0
75-79	35.53329249554069	37.4	35.4	37.6	31.6	38.4
80-84	35.645723178325774	37.2	35.8	38.2	31.2	38.6
85-89	35.36294072412954	37.2	35.4	37.8	31.0	38.2
90-94	35.38534206288022	37.0	35.0	38.2	30.8	38.4
95-99	35.21324935874087	37.4	35.2	37.6	31.0	38.2
100	34.14929957792497	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	15.0
2	42.0
3	207.0
4	0.0
5	18.0
6	39.0
7	196.0
8	356.0
9	18889.0
10	967.0
11	1495.0
12	2408.0
13	4690.0
14	6144.0
15	7312.0
16	8113.0
17	9894.0
18	12193.0
19	15015.0
20	17518.0
21	17855.0
22	18231.0
23	21050.0
24	25948.0
25	34170.0
26	45684.0
27	63329.0
28	86422.0
29	115563.0
30	151961.0
31	199522.0
32	257864.0
33	345035.0
34	508314.0
35	911565.0
36	2750526.0
37	4118380.0
38	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.24326736220118	20.3681402021724	22.476643668327682	37.91194876729874
2	17.028667141355296	53.8112929224827	14.796161479273815	14.363878456888182
3	18.515873397380872	36.271902721919616	30.23065293088058	14.981570949818929
4	32.9020641383637	25.60983110476917	21.691033258296123	19.79707149857101
5	19.696684901592345	28.143903584651635	27.709521922643393	24.449889591112623
6	19.501962158316644	30.20405296389826	26.985807314985355	23.308177562799745
7	21.166803047881164	28.83962627881158	28.428755813761065	21.564814859546193
8	20.409667565770963	28.265096693029225	29.899550509524985	21.425685231674827
9	21.024991285958205	29.03082894873039	27.994189473848675	21.949990291462726
10-14	21.247075123719206	28.308026670311815	27.591836083536464	22.85306212243251
15-19	20.789919404947646	28.094473621667948	28.08443823520902	23.03116873817539
20-24	21.026917434982735	27.38336650716699	28.350390885978328	23.239325171871943
25-29	21.19438204660188	27.68434523696499	28.979790644516484	22.141482071916645
30-34	21.840165227243187	28.09798746093203	28.242629580957303	21.81921773086749
35-39	21.893973018248005	26.979988016587292	29.235122261518224	21.89091670364648
40-44	21.973469607529665	28.07344334858637	27.900393459880114	22.052693584003848
45-49	21.557878151074615	28.719476896552404	28.51080556560982	21.211839386763163
50-54	21.90844379301635	28.145731502121134	28.186506134021933	21.759318570840584
55-59	21.40812378213545	28.887986279208995	27.94269053686451	21.761199401791046
60-64	21.776557393582916	28.364722753865568	28.796026695225923	21.062693157325594
65-69	21.665913013122555	29.379765236564502	28.38041208093084	20.5739096693821
70-74	21.544963817484966	28.88450522562985	27.970529628081113	21.600001328804066
75-79	22.078368867385905	28.805599254107904	28.103394315366394	21.012637563139798
80-84	21.41032311390387	28.726812204719508	28.3968438951757	21.46602078620092
85-89	21.437782134402728	28.592784057641474	28.611866416165388	21.357567391790408
90-94	21.432265991376788	28.24818151045676	28.662682818666426	21.656869679500026
95-99	21.660000702957667	27.690817444111786	28.837182795673392	21.811999057257157
100	22.957949416975364	27.850949659105318	27.31249714081191	21.878603783107412
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	57.15521612949992
2	31.392545598243483
3	33.4974443471998
4	52.69913563693471
5	44.14657449270497
6	42.81013972111639
7	42.731617907427356
8	41.83535279744579
9	42.974981577420934
10-14	44.100137246151725
15-19	43.821088143123035
20-24	44.26624260685468
25-29	43.33586411851852
30-34	43.65938295811067
35-39	43.78488972189449
40-44	44.02616319153351
45-49	42.76971753783778
50-54	43.66776236385693
55-59	43.169323183926494
60-64	42.83925055090851
65-69	42.23982268250466
70-74	43.14496514628903
75-79	43.0910064305257
80-84	42.87634390010479
85-89	42.795349526193135
90-94	43.089135670876814
95-99	43.47199976021482
100	44.836553200082776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19374.0
1	19374.5
2	19376.5
3	19380.5
4	19384.0
5	19390.5
6	19409.0
7	19435.0
8	19472.5
9	19497.5
10	19585.5
11	19698.5
12	19752.5
13	19887.0
14	20008.5
15	20972.0
16	21698.0
17	21259.0
18	21828.5
19	23767.0
20	26375.0
21	29972.5
22	37540.5
23	53310.0
24	85945.5
25	108347.5
26	130200.0
27	163484.0
28	171147.5
29	196633.5
30	248316.0
31	278351.0
32	291418.5
33	322230.5
34	356468.0
35	349875.0
36	329683.0
37	310817.5
38	351043.5
39	391062.5
40	393255.0
41	418087.5
42	410053.0
43	403683.5
44	409910.0
45	380764.0
46	336183.5
47	292564.5
48	276197.0
49	298510.5
50	310357.0
51	313880.5
52	263196.0
53	166003.0
54	142534.0
55	143821.5
56	111843.5
57	94839.0
58	121628.0
59	119973.0
60	116476.5
61	150318.5
62	138593.5
63	121391.0
64	112254.5
65	102675.5
66	103395.5
67	92306.0
68	72929.0
69	64668.0
70	61616.0
71	54216.0
72	62925.5
73	47682.0
74	23781.0
75	28648.0
76	31044.0
77	29208.0
78	25302.0
79	24737.5
80	22409.0
81	19367.0
82	17753.0
83	17910.5
84	19507.5
85	18831.5
86	19348.0
87	17421.0
88	16045.5
89	17110.0
90	18669.0
91	20354.0
92	17277.5
93	14159.5
94	14083.0
95	14059.5
96	14056.5
97	14052.5
98	14037.5
99	14018.5
100	14012.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05285907451615741
2	0.0072766173281265546
3	0.0019063641096003575
4	0.007144426163688131
5	0.004401387226969074
6	7.804211234531744E-4
7	3.1863867689709246E-4
8	1.4390581980832772E-4
9	6.167410028023683E-5
10-14	0.0012149895917947457
15-19	0.017328602633038814
20-24	0.01592222682133398
25-29	0.008508700835799009
30-34	0.007923432497366932
35-39	0.005285420388085316
40-44	0.012520616335397137
45-49	0.01149333020460501
50-54	0.010398435185964186
55-59	0.010811181876253538
60-64	0.052463074582800036
65-69	0.04245893373554075
70-74	0.026978659298221883
75-79	0.07367934185138408
80-84	0.06277633463398029
85-89	0.014760228351828693
90-94	0.006907546058198443
95-99	0.009562266797950534
100	0.00824059354974918
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	19659.0
2-3	1424.0
4-5	17525.0
6-7	9747.0
8-9	28.0
10-11	57.0
12-13	111.0
14-15	303.0
16-17	309.0
18-19	5621.0
20-21	55017.0
22-23	7321.0
24-25	8761.0
26-27	4108.0
28-29	4123.0
30-31	7089.0
32-33	3068.0
34-35	4706.0
36-37	3126.0
38-39	2697.0
40-41	5222.0
42-43	3158.0
44-45	3707.0
46-47	6390.0
48-49	3148.0
50-51	37589.0
52-53	5059.0
54-55	46428.0
56-57	28634.0
58-59	84599.0
60-61	4335.0
62-63	20200.0
64-65	11707.0
66-67	15947.0
68-69	18844.0
70-71	56767.0
72-73	51514.0
74-75	31170.0
76-77	37833.0
78-79	12822.0
80-81	24761.0
82-83	27565.0
84-85	14353.0
86-87	5449.0
88-89	9870.0
90-91	20255.0
92-93	7985.0
94-95	6082.0
96-97	210702.0
98-99	0.0
100-101	8810045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.07845536352704
#Duplication Level	Relative count
1	100.0
2	6.848503348835539
3	2.026060464587336
4	0.9935776576705991
5	0.5584385268714873
6	0.3645113213984306
7	0.2753287485111299
8	0.24181046608368797
9	0.19153304244252511
10++	8.208387950177467
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	26051	0.2664535120395543	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	25814	0.26402944070435125	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	25372	0.25950859880494304	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	25165	0.2573913719425505	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	19343	0.197843087919124	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	19198	0.19636000630053985	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	19113	0.19549061362757672	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	18867	0.19297448895053054	No Hit
*	18314	0.18731832250172345	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	16618	0.16997138163883588	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	16340	0.16712795619079182	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	16001	0.16366061364803303	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	15873	0.1623514105640415	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	15871	0.16233095426585414	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	15788	0.16148201789107838	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	15771	0.16130813935648577	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	15724	0.16082741634908262	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	15696	0.1605410281744595	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	15408	0.1575953212354786	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	15262	0.15610201146780078	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	13933	0.1425088013222951	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	13817	0.1413223360274278	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	13801	0.14115868564192885	No Hit
TGAGACCGCACCAGCGACACA	13514	0.13822320685204165	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	13470	0.13777316829191957	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	13387	0.1369242319171438	No Hit
TGTGTCGCTGGTGCGGTCTCA	13338	0.1364230526115533	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	12978	0.13274091893782716	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	12870	0.13163627883570933	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	12738	0.13028616315534308	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	12610	0.12897696007135157	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	11846	0.12116265416377721	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	11706	0.11973071329066148	No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA	11590	0.11854424799579417	No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT	11491	0.1175316612355195	No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA	11274	0.11531215288219014	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	11115	0.11368587717629443	No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT	10738	0.10982986496797566	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	10629	0.10871499671676414	No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG	10611	0.10853089003307784	No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC	10571	0.10812176406933048	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	10537	0.10777400700014524	No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC	10535	0.10775355070195788	No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG	10509	0.10748761882552209	No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG	10495	0.10734442473821053	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	10465	0.1070375802654	No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC	10296	0.10530902306856746	No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG	10291	0.10525788232309904	No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA	10281	0.10515560083216222	No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC	10266	0.10500217859575696	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	10073	0.10302814582067599	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	10023	0.1025167383659918	No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG	9975	0.102025787209495	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	9903	0.10128936047474976	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	5724620	3.3382175	6.489592	2
TTTTT	5198145	3.273735	4.1566944	60-64
GGGGG	1303835	3.1264358	8.077449	4
GGAGG	1521585	2.7498646	5.184495	4
CCAGC	1531250	2.6432786	8.3331585	5
CCTCC	1518605	2.6217282	9.763897	6
CCCCA	1531020	2.6027849	5.6884093	1
CCTGG	1457895	2.595063	5.435855	9
CAGCC	1471780	2.5406203	5.3758807	6
TGGGG	1330050	2.4410038	10.342536	3
CTCCC	1366880	2.3597894	5.2128105	3
GGCAG	1253225	2.2305129	5.9569163	8
GCTGG	1232585	2.2278092	7.066234	7
GGGGA	1185960	2.143311	5.5793877	7
GAGGG	1180940	2.1342387	7.480356	9
CCACC	1221475	2.076548	5.211939	1
CCCTC	1191655	2.0572798	5.513981	5
GCCAG	1122600	1.967711	7.5417466	4
AAGAA	2521345	1.950797	5.3925896	2
CCCAC	1145495	1.9473795	6.9345665	9
TCCAG	1434065	1.9238833	6.168261	7
TGGCT	1367625	1.8919158	6.094239	3
GAGGA	1384255	1.8854691	5.2331114	1
TGCAG	1381180	1.8814799	6.1349115	3
CACCA	1445115	1.8801284	5.9988394	8
TGGTG	1329335	1.8672765	5.096312	9
TGTTT	2236045	1.8399329	5.8751864	3
TCCTC	1360625	1.8255525	5.9505515	5
GCCCA	1037630	1.7911806	5.726553	4
AGGGG	973745	1.7597882	7.759351	6
GGCTC	984565	1.7525326	6.5196633	4
CCAGA	1310760	1.7316003	5.276053	8
AAACA	2271065	1.7304933	5.024865	2
AGGAA	1661100	1.7052473	5.0648184	2
GGCCC	712930	1.6328847	5.10882	1
GAAAG	1558390	1.5998075	5.1042733	5
GCTCC	875865	1.5353925	6.8490853	2
ATGGA	1445260	1.5066867	9.975584	2
TGCCC	855690	1.5000256	10.96967	3
GATGG	1077165	1.4899476	5.375368	1
CATGG	1083885	1.4764968	41.03609	1
TAAAA	2471700	1.46369	8.421302	1
GCAGT	1001380	1.3641063	6.0703936	4
TGCTC	969060	1.320219	5.274113	1
ATGTG	1234015	1.30642	7.127659	2
TGGGT	901945	1.2669348	6.218194	3
ATGAA	1580705	1.241984	8.83396	2
ATGGC	906295	1.2345791	9.861115	2
AGACA	1220970	1.2344035	5.4002333	2
TGAGA	1178440	1.2285262	5.4589353	1
ATGGG	880555	1.2179942	14.729165	2
ATGCC	903495	1.2120923	11.453701	2
GAGAC	826765	1.1090369	5.3794637	2
CATGT	1048025	1.0926841	32.527866	1
CATGA	1042230	1.0700427	29.85829	1
ATGTT	1290670	1.0458063	8.097464	2
TAAAG	1301350	1.0224905	7.2181053	1
ATGCT	975100	1.0166516	7.3152337	2
ATGGT	947380	1.0029669	7.3777175	2
AGACT	958260	0.98383194	5.162807	1
ATGAC	954350	0.9798176	7.5495787	2
AGACC	717630	0.9480365	5.0267076	3
ATGAT	1182830	0.9437847	6.6456113	2
ATGTA	1178685	0.94047743	9.288199	2
CATGC	686755	0.92132264	31.44624	1
GCGCC	384895	0.88155806	5.726927	8
TAAGA	1028440	0.808061	5.132835	1
CGCTG	451475	0.80362856	5.084466	6
ATGCA	731695	0.7512209	8.682851	2
TAACT	953665	0.7493885	5.8696923	1
TAAGT	927090	0.7397288	6.6269255	1
TAAAC	916935	0.7095194	5.655586	1
TAAGG	619755	0.64609593	5.602846	1
CGCCT	345500	0.605662	5.4879947	9
TGCCG	311045	0.5536622	7.928434	5
CCGTC	243705	0.4272152	5.3205795	7
GCCGT	237090	0.4220218	5.407526	6
>>END_MODULE