FastQCFastQC Report
Wed 20 Mar 2013
8016-8-48.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-48.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9776940
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 26051 0.2664535120395543 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 25814 0.26402944070435125 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 25372 0.25950859880494304 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 25165 0.2573913719425505 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 19343 0.197843087919124 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 19198 0.19636000630053985 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 19113 0.19549061362757672 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 18867 0.19297448895053054 No Hit
* 18314 0.18731832250172345 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 16618 0.16997138163883588 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 16340 0.16712795619079182 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 16001 0.16366061364803303 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 15873 0.1623514105640415 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 15871 0.16233095426585414 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 15788 0.16148201789107838 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 15771 0.16130813935648577 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 15724 0.16082741634908262 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 15696 0.1605410281744595 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 15408 0.1575953212354786 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 15262 0.15610201146780078 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 13933 0.1425088013222951 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 13817 0.1413223360274278 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 13801 0.14115868564192885 No Hit
TGAGACCGCACCAGCGACACA 13514 0.13822320685204165 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 13470 0.13777316829191957 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 13387 0.1369242319171438 No Hit
TGTGTCGCTGGTGCGGTCTCA 13338 0.1364230526115533 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 12978 0.13274091893782716 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 12870 0.13163627883570933 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 12738 0.13028616315534308 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 12610 0.12897696007135157 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 11846 0.12116265416377721 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 11706 0.11973071329066148 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 11590 0.11854424799579417 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 11491 0.1175316612355195 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 11274 0.11531215288219014 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 11115 0.11368587717629443 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 10738 0.10982986496797566 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 10629 0.10871499671676414 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 10611 0.10853089003307784 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 10571 0.10812176406933048 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 10537 0.10777400700014524 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 10535 0.10775355070195788 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 10509 0.10748761882552209 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 10495 0.10734442473821053 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 10465 0.1070375802654 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 10296 0.10530902306856746 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 10291 0.10525788232309904 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 10281 0.10515560083216222 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 10266 0.10500217859575696 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 10073 0.10302814582067599 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 10023 0.1025167383659918 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 9975 0.102025787209495 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 9903 0.10128936047474976 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5724620 3.3382175 6.489592 2
TTTTT 5198145 3.273735 4.1566944 60-64
GGGGG 1303835 3.1264358 8.077449 4
GGAGG 1521585 2.7498646 5.184495 4
CCAGC 1531250 2.6432786 8.3331585 5
CCTCC 1518605 2.6217282 9.763897 6
CCCCA 1531020 2.6027849 5.6884093 1
CCTGG 1457895 2.595063 5.435855 9
CAGCC 1471780 2.5406203 5.3758807 6
TGGGG 1330050 2.4410038 10.342536 3
CTCCC 1366880 2.3597894 5.2128105 3
GGCAG 1253225 2.2305129 5.9569163 8
GCTGG 1232585 2.2278092 7.066234 7
GGGGA 1185960 2.143311 5.5793877 7
GAGGG 1180940 2.1342387 7.480356 9
CCACC 1221475 2.076548 5.211939 1
CCCTC 1191655 2.0572798 5.513981 5
GCCAG 1122600 1.967711 7.5417466 4
AAGAA 2521345 1.950797 5.3925896 2
CCCAC 1145495 1.9473795 6.9345665 9
TCCAG 1434065 1.9238833 6.168261 7
TGGCT 1367625 1.8919158 6.094239 3
GAGGA 1384255 1.8854691 5.2331114 1
TGCAG 1381180 1.8814799 6.1349115 3
CACCA 1445115 1.8801284 5.9988394 8
TGGTG 1329335 1.8672765 5.096312 9
TGTTT 2236045 1.8399329 5.8751864 3
TCCTC 1360625 1.8255525 5.9505515 5
GCCCA 1037630 1.7911806 5.726553 4
AGGGG 973745 1.7597882 7.759351 6
GGCTC 984565 1.7525326 6.5196633 4
CCAGA 1310760 1.7316003 5.276053 8
AAACA 2271065 1.7304933 5.024865 2
AGGAA 1661100 1.7052473 5.0648184 2
GGCCC 712930 1.6328847 5.10882 1
GAAAG 1558390 1.5998075 5.1042733 5
GCTCC 875865 1.5353925 6.8490853 2
ATGGA 1445260 1.5066867 9.975584 2
TGCCC 855690 1.5000256 10.96967 3
GATGG 1077165 1.4899476 5.375368 1
CATGG 1083885 1.4764968 41.03609 1
TAAAA 2471700 1.46369 8.421302 1
GCAGT 1001380 1.3641063 6.0703936 4
TGCTC 969060 1.320219 5.274113 1
ATGTG 1234015 1.30642 7.127659 2
TGGGT 901945 1.2669348 6.218194 3
ATGAA 1580705 1.241984 8.83396 2
ATGGC 906295 1.2345791 9.861115 2
AGACA 1220970 1.2344035 5.4002333 2
TGAGA 1178440 1.2285262 5.4589353 1
ATGGG 880555 1.2179942 14.729165 2
ATGCC 903495 1.2120923 11.453701 2
GAGAC 826765 1.1090369 5.3794637 2
CATGT 1048025 1.0926841 32.527866 1
CATGA 1042230 1.0700427 29.85829 1
ATGTT 1290670 1.0458063 8.097464 2
TAAAG 1301350 1.0224905 7.2181053 1
ATGCT 975100 1.0166516 7.3152337 2
ATGGT 947380 1.0029669 7.3777175 2
AGACT 958260 0.98383194 5.162807 1
ATGAC 954350 0.9798176 7.5495787 2
AGACC 717630 0.9480365 5.0267076 3
ATGAT 1182830 0.9437847 6.6456113 2
ATGTA 1178685 0.94047743 9.288199 2
CATGC 686755 0.92132264 31.44624 1
GCGCC 384895 0.88155806 5.726927 8
TAAGA 1028440 0.808061 5.132835 1
CGCTG 451475 0.80362856 5.084466 6
ATGCA 731695 0.7512209 8.682851 2
TAACT 953665 0.7493885 5.8696923 1
TAAGT 927090 0.7397288 6.6269255 1
TAAAC 916935 0.7095194 5.655586 1
TAAGG 619755 0.64609593 5.602846 1
CGCCT 345500 0.605662 5.4879947 9
TGCCG 311045 0.5536622 7.928434 5
CCGTC 243705 0.4272152 5.3205795 7
GCCGT 237090 0.4220218 5.407526 6
Produced by FastQC (version 0.10.0)