##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-49.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4774586	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.95611577632071	24.0	24.0	31.0	23.0	31.0
2	31.01975287081395	31.0	31.0	32.0	29.0	33.0
3	32.66733895008742	33.0	33.0	34.0	29.0	35.0
4	33.884032215525	34.0	33.0	36.0	29.0	37.0
5	35.42050919660676	36.0	35.0	37.0	33.0	37.0
6	35.75136572548622	36.0	36.0	38.0	33.0	38.0
7	35.76609141351896	37.0	35.0	37.0	34.0	37.0
8	35.61868306309943	36.0	36.0	37.0	32.0	38.0
9	35.64219168377471	37.0	36.0	37.0	31.0	38.0
10-14	36.00499621341966	37.0	36.0	37.4	32.4	38.0
15-19	36.99392058231655	38.0	37.2	38.4	33.4	39.4
20-24	36.63138001075477	37.4	36.0	38.8	32.8	39.8
25-29	36.29066360094304	37.6	36.2	38.0	32.8	38.2
30-34	36.13100279325504	37.6	35.6	38.2	32.6	38.6
35-39	36.162515460733026	37.8	36.4	38.0	31.6	38.6
40-44	36.095438877739994	37.4	36.0	38.4	32.4	38.8
45-49	36.051025883055914	37.6	35.8	38.2	32.2	38.6
50-54	36.26335741712458	37.6	36.2	38.4	33.0	39.0
55-59	35.98276254878496	37.6	35.8	38.2	32.4	38.6
60-64	35.99640300849359	37.6	35.6	38.2	32.6	38.6
65-69	35.80577567164172	37.4	36.0	38.2	31.6	38.6
70-74	35.84630009398541	37.4	36.0	38.4	32.0	39.0
75-79	35.55454925009225	37.4	35.4	37.6	32.2	38.4
80-84	35.66435297986187	37.2	35.8	38.2	31.2	38.6
85-89	35.37641548661646	37.2	35.4	37.8	31.0	38.2
90-94	35.3992304019404	37.0	35.0	38.2	31.6	38.4
95-99	35.225268452401345	37.4	35.2	37.6	31.0	38.2
100	34.140559953185246	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	6.0
2	26.0
3	126.0
4	1.0
5	11.0
6	28.0
7	118.0
8	166.0
9	9932.0
10	523.0
11	702.0
12	1081.0
13	1913.0
14	2448.0
15	3154.0
16	3900.0
17	4663.0
18	5822.0
19	6942.0
20	7942.0
21	8215.0
22	8958.0
23	10461.0
24	13032.0
25	16975.0
26	23193.0
27	31709.0
28	43521.0
29	57870.0
30	76966.0
31	101426.0
32	127447.0
33	168095.0
34	247051.0
35	443311.0
36	1346777.0
37	2000068.0
38	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.44820018213724	19.69530544104026	21.88607582444188	38.97041855238063
2	17.243143366469983	52.397754018508266	15.743030429237056	14.616072185784686
3	18.821332209177044	35.46105663413164	30.334248949672453	15.383362207018866
4	33.18122100905702	25.16768756226595	21.487633331037298	20.163458097639733
5	19.993221637090492	27.89631253266221	27.20581683801798	24.904648992229315
6	19.88787252876955	29.87054627238362	26.564089089443232	23.677492109403598
7	21.25176326812318	28.65064472085934	28.095539246375978	22.0020527646415
8	20.775323218857782	28.068769969741215	29.42760109185552	21.72830571954548
9	20.914951911235327	29.21761700132294	27.59064835826151	22.276782729180226
10-14	21.529589196305604	28.038744307865475	27.373302368674512	23.058364127154405
15-19	20.92868600438923	27.828774917594522	27.848879131846772	23.393659946169475
20-24	21.244577214649823	27.250001137621254	28.1242301121584	23.381191535570526
25-29	21.394455538638155	27.502198100823822	28.697273843048137	22.406072517489886
30-34	22.09987857789176	27.898756912819728	27.908763314268782	22.092601195019725
35-39	22.027086492567754	26.834084396476186	28.997272781134853	22.14155632982121
40-44	22.017012500783693	27.745992360572753	27.930796229800787	22.306198908842774
45-49	21.678401552851458	28.516332535774367	28.269862866328978	21.535403045045193
50-54	22.175203932651765	27.87142927048126	28.02816757268523	21.92519922418174
55-59	21.557327872462544	28.744973634883454	27.77302764479139	21.92467084786261
60-64	21.970060679942613	28.248486728219113	28.556770721118756	21.224681870719515
65-69	21.88478282039664	29.11119491441702	28.180774238061534	20.823248027124798
70-74	21.64625391025406	28.70667761628054	27.777644128021002	21.8694243454444
75-79	22.308091463002196	28.604713133783736	27.89610974382512	21.19108565938895
80-84	21.546792975207786	28.530114370040348	28.225787495528422	21.697305159223447
85-89	21.623706646605676	28.399272604716717	28.353092014633113	21.62392873404449
90-94	21.683919835604698	28.175817613266695	28.32645238740478	21.813810163723822
95-99	21.83573648559444	27.49136737820007	28.670390268342743	22.002505867862745
100	23.05309908936136	27.67031993526794	27.168291926849413	22.108289048521286
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	58.41861873451786
2	31.85921555225467
3	34.20469441619591
4	53.344679106696745
5	44.89787062931981
6	43.56536463817314
7	43.25381603276468
8	42.50362893840327
9	43.191734640415554
10-14	44.58795332346001
15-19	44.322345950558706
20-24	44.625768750220345
25-29	43.800528056128044
30-34	44.19247977291148
35-39	44.16864282238897
40-44	44.32321140962647
45-49	43.213804597896655
50-54	44.1004031568335
55-59	43.48199872032515
60-64	43.19474255066213
65-69	42.70803084752144
70-74	43.51567825569846
75-79	43.499177122391146
80-84	43.24409813443123
85-89	43.24763538065016
90-94	43.49772999932852
95-99	43.83824235345718
100	45.161388137882646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10262.0
1	10261.5
2	10262.0
3	10261.0
4	10261.5
5	10266.0
6	10273.5
7	10288.0
8	10311.5
9	10331.0
10	10358.5
11	10386.0
12	10412.5
13	10481.5
14	10507.0
15	10821.5
16	11103.5
17	11137.0
18	11477.0
19	12332.0
20	13661.5
21	15238.0
22	19592.0
23	26454.0
24	40219.5
25	51949.0
26	64179.0
27	79285.5
28	81754.0
29	93961.0
30	117120.5
31	131582.0
32	139383.5
33	151917.0
34	166049.5
35	165271.0
36	158711.5
37	149071.5
38	165735.5
39	185608.0
40	188717.5
41	200594.0
42	194836.0
43	194431.5
44	196949.5
45	183095.5
46	165986.5
47	141590.0
48	132772.5
49	145678.0
50	152150.5
51	154364.0
52	131731.0
53	85461.5
54	71149.5
55	70848.0
56	57198.0
57	49950.0
58	63960.0
59	63951.5
60	64757.5
61	82194.0
62	75037.0
63	68539.5
64	64496.5
65	57083.5
66	56941.0
67	52574.5
68	41121.5
69	36082.5
70	36265.5
71	32762.0
72	36772.5
73	29656.5
74	18069.0
75	22436.5
76	25297.0
77	24233.5
78	22450.0
79	21802.5
80	20548.5
81	18948.5
82	18129.0
83	18390.5
84	18599.0
85	17687.0
86	19713.5
87	19523.0
88	19323.0
89	21303.0
90	24229.0
91	27226.0
92	24309.0
93	21347.5
94	21271.5
95	21252.0
96	21248.0
97	21245.5
98	21244.5
99	21246.0
100	21244.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05502047716807279
2	0.007535578319952259
3	0.0019314065174053318
4	0.007223588544396511
5	0.004549595328239612
6	9.927172572278264E-4
7	3.3852940709748063E-4
8	1.2695978294955507E-4
9	4.2320464949556125E-5
10-14	0.0013204130353544822
15-19	0.01783618618489607
20-24	0.016094218488987174
25-29	0.008719119536142841
30-34	0.008327297466140035
35-39	0.005257692773946739
40-44	0.012306475221387114
45-49	0.011669187779550193
50-54	0.010920737715610404
55-59	0.010576139535690136
60-64	0.054670521589904306
65-69	0.04365335644144879
70-74	0.02679723756378987
75-79	0.07562587000360549
80-84	0.06596598119336071
85-89	0.01442453775655613
90-94	0.006918882826810043
95-99	0.009606223544048402
100	0.007603432926642966
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	10519.0
2-3	1891.0
4-5	27696.0
6-7	8574.0
8-9	60.0
10-11	19.0
12-13	130.0
14-15	180.0
16-17	197.0
18-19	2143.0
20-21	27918.0
22-23	2881.0
24-25	3112.0
26-27	1644.0
28-29	1689.0
30-31	3080.0
32-33	1500.0
34-35	1670.0
36-37	1613.0
38-39	1520.0
40-41	3036.0
42-43	1931.0
44-45	1613.0
46-47	2815.0
48-49	1238.0
50-51	18044.0
52-53	2318.0
54-55	20788.0
56-57	15489.0
58-59	41123.0
60-61	2087.0
62-63	11830.0
64-65	5460.0
66-67	7855.0
68-69	9217.0
70-71	25898.0
72-73	23511.0
74-75	15524.0
76-77	16470.0
78-79	5464.0
80-81	9956.0
82-83	11400.0
84-85	8005.0
86-87	2943.0
88-89	4808.0
90-91	10521.0
92-93	4211.0
94-95	3415.0
96-97	102043.0
98-99	0.0
100-101	4287537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.33662604671444
#Duplication Level	Relative count
1	100.0
2	6.6873746707034165
3	2.0312192820351513
4	1.0741949435772422
5	0.7722250619274171
6	0.7588566036252113
7	0.712460189517556
8	0.7454881453230056
9	0.7753705815279361
10++	13.278810993591003
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	10457	0.21901375323431183	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10360	0.2169821634797237	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	10316	0.21606061761166306	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10227	0.2141965816512678	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	10168	0.21296087241909561	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	10096	0.21145288827136005	No Hit
*	9616	0.2013996606197899	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	9065	0.18985939304475824	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	8938	0.18719947656194694	No Hit
TGAGACCGCACCAGCGACACA	8275	0.1733134558682156	No Hit
TGTGTCGCTGGTGCGGTCTCA	8178	0.17128186611362745	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	7602	0.1592179929317432	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	7547	0.15806606059666745	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	7415	0.15530142299248564	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	7222	0.15125918770758345	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	7160	0.14996064580258894	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	7047	0.14759394845961513	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	7044	0.14753111578679282	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	7037	0.1473845062168741	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	7020	0.14702845440421433	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	7013	0.1468818448342956	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	6998	0.146567681470184	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	6975	0.1460859643118796	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	6938	0.14531102801373774	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	6934	0.1452272511166413	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	6892	0.14434759369712893	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	6751	0.14139445807448017	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	6702	0.14036819108504905	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	6682	0.13994930659956695	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	6414	0.13433625449410694	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	6397	0.13398020268144714	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	6344	0.13287015879491962	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	6170	0.1292258637712254	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	6124	0.12826242945461658	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	6092	0.12759221427784523	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	6050	0.12671255685833285	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	5900	0.12357092321721716	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	5833	0.12216766019085214	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	5703	0.11944491103521855	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	5687	0.11910980344683288	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	5671	0.11877469585844719	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	5632	0.11795787111175712	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	5593	0.11714104636506704	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	5545	0.11613572359991002	No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA	5533	0.11588439290862076	No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC	5502	0.11523512195612354	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	5500	0.11519323350757532	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	5486	0.11490001436773785	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	5465	0.11446018565798165	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	5292	0.11083683485856156	No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA	5116	0.10715065138631914	No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT	5076	0.10631288241535497	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	5069	0.10616627284543623	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	5040	0.10555889034148722	No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA	4974	0.10417657153939629	No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG	4938	0.10342257946552853	No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC	4928	0.10321313722278748	No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG	4923	0.10310841610141697	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	4911	0.1028570854101277	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	4909	0.1028151969615795	No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG	4906	0.10275236428875717	No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT	4887	0.1023544240275492	No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC	4868	0.10195648376634121	No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC	4810	0.10074171875844314	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	4781	0.10013433625449411	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2692550	3.3585513	6.6275206	2
TTTTT	2471925	3.32701	3.9891906	95-96
GGGGG	652690	3.0819795	8.253633	4
GGAGG	765985	2.7715077	5.7345796	4
CCAGG	766005	2.674662	5.20214	5
CCAGC	770035	2.641305	7.931545	5
CCTCC	770465	2.635954	9.123311	6
CCCCA	769920	2.594325	5.6895995	1
CCTGG	730265	2.5889516	5.1250153	9
CAGCC	744730	2.554506	5.995188	6
TGGGG	663610	2.4378943	9.939907	3
GCTGG	614215	2.2166295	7.6656413	7
GGCAG	613910	2.1820834	5.1546826	8
GGGGA	588505	2.129345	5.530319	7
GAGGG	587255	2.1248221	6.568522	9
CCACC	613060	2.065769	5.0440483	1
CTCCT	741175	2.008233	5.455347	4
GCCAG	565790	1.9755704	7.656275	4
CCCAC	573900	1.9338155	6.201914	9
TCCAG	709055	1.9261782	6.2383146	7
TGGCT	678120	1.9039629	6.5011063	3
CACCA	716175	1.8823496	5.4494534	8
TGGTG	657610	1.8795311	5.497449	9
TCCTC	690825	1.8718085	6.547982	5
TGCAG	675385	1.8676573	5.298029	3
TGTTT	1070010	1.8510897	5.7772827	3
GGCTC	514810	1.8251158	6.820912	4
GCCCA	518860	1.7797467	5.680853	4
AGGGG	483240	1.7484721	7.1361747	6
GGCCC	373060	1.6699892	6.269019	1
GCTCC	445550	1.5517101	6.7618766	2
TAAAA	1187795	1.5043046	8.541426	1
ATGGA	693970	1.4968863	9.969441	2
TGCCC	428520	1.4923999	11.208805	3
GATGG	527555	1.4850558	5.52745	1
CATGG	522140	1.4438856	40.56618	1
TGCTC	469755	1.295669	5.387774	1
ATGTG	585190	1.281596	7.522701	2
TGGGT	447825	1.2799395	6.1892548	3
ATGAA	765305	1.2648988	8.76721	2
TGAGA	575995	1.2424154	5.738297	1
ATGGC	445550	1.2320894	10.092716	2
AGACA	590145	1.231605	5.237671	2
ATGGG	427205	1.2025727	14.393996	2
ATGCC	439880	1.1949528	11.49219	2
GAGAC	409090	1.1141881	5.7793355	2
CATGT	501500	1.0789367	31.965643	1
CATGA	502870	1.0655519	29.533949	1
ATGTT	611630	1.0421308	7.858466	2
ATGCT	475135	1.0222145	7.7080874	2
TAAAG	617245	1.0201848	6.5629106	1
ATGGT	457015	1.0008862	7.568521	2
AGACT	469695	0.99525607	5.24882	1
ATGAC	456295	0.96686214	7.4203053	2
ATGAT	572860	0.9613374	6.8706627	2
ATGTA	565510	0.9490031	9.022525	2
AGACC	354040	0.9472459	5.4923253	3
CATGC	337700	0.91737646	31.591654	1
GCGCC	203750	0.9120792	5.7455397	8
CGCTG	229435	0.813398	5.5392656	6
ATGCA	353110	0.74821925	8.478378	2
TAAGT	444080	0.745227	6.470449	1
TAACT	448840	0.7399283	5.4241233	1
TAAAC	432620	0.70242274	5.2383027	1
TGCCG	154420	0.54745317	7.131109	5
>>END_MODULE