FastQCFastQC Report
Wed 20 Mar 2013
8016-8-49.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-49.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4774586
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10457 0.21901375323431183 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10360 0.2169821634797237 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10316 0.21606061761166306 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10227 0.2141965816512678 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 10168 0.21296087241909561 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10096 0.21145288827136005 No Hit
* 9616 0.2013996606197899 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 9065 0.18985939304475824 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8938 0.18719947656194694 No Hit
TGAGACCGCACCAGCGACACA 8275 0.1733134558682156 No Hit
TGTGTCGCTGGTGCGGTCTCA 8178 0.17128186611362745 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7602 0.1592179929317432 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7547 0.15806606059666745 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7415 0.15530142299248564 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 7222 0.15125918770758345 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7160 0.14996064580258894 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 7047 0.14759394845961513 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 7044 0.14753111578679282 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 7037 0.1473845062168741 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7020 0.14702845440421433 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 7013 0.1468818448342956 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 6998 0.146567681470184 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6975 0.1460859643118796 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6938 0.14531102801373774 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6934 0.1452272511166413 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6892 0.14434759369712893 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 6751 0.14139445807448017 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 6702 0.14036819108504905 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6682 0.13994930659956695 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6414 0.13433625449410694 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6397 0.13398020268144714 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6344 0.13287015879491962 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 6170 0.1292258637712254 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 6124 0.12826242945461658 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6092 0.12759221427784523 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6050 0.12671255685833285 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5900 0.12357092321721716 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5833 0.12216766019085214 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5703 0.11944491103521855 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5687 0.11910980344683288 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5671 0.11877469585844719 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5632 0.11795787111175712 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 5593 0.11714104636506704 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5545 0.11613572359991002 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 5533 0.11588439290862076 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 5502 0.11523512195612354 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 5500 0.11519323350757532 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 5486 0.11490001436773785 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 5465 0.11446018565798165 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 5292 0.11083683485856156 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 5116 0.10715065138631914 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 5076 0.10631288241535497 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5069 0.10616627284543623 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5040 0.10555889034148722 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 4974 0.10417657153939629 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 4938 0.10342257946552853 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 4928 0.10321313722278748 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 4923 0.10310841610141697 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 4911 0.1028570854101277 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 4909 0.1028151969615795 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 4906 0.10275236428875717 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 4887 0.1023544240275492 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 4868 0.10195648376634121 No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC 4810 0.10074171875844314 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4781 0.10013433625449411 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2692550 3.3585513 6.6275206 2
TTTTT 2471925 3.32701 3.9891906 95-96
GGGGG 652690 3.0819795 8.253633 4
GGAGG 765985 2.7715077 5.7345796 4
CCAGG 766005 2.674662 5.20214 5
CCAGC 770035 2.641305 7.931545 5
CCTCC 770465 2.635954 9.123311 6
CCCCA 769920 2.594325 5.6895995 1
CCTGG 730265 2.5889516 5.1250153 9
CAGCC 744730 2.554506 5.995188 6
TGGGG 663610 2.4378943 9.939907 3
GCTGG 614215 2.2166295 7.6656413 7
GGCAG 613910 2.1820834 5.1546826 8
GGGGA 588505 2.129345 5.530319 7
GAGGG 587255 2.1248221 6.568522 9
CCACC 613060 2.065769 5.0440483 1
CTCCT 741175 2.008233 5.455347 4
GCCAG 565790 1.9755704 7.656275 4
CCCAC 573900 1.9338155 6.201914 9
TCCAG 709055 1.9261782 6.2383146 7
TGGCT 678120 1.9039629 6.5011063 3
CACCA 716175 1.8823496 5.4494534 8
TGGTG 657610 1.8795311 5.497449 9
TCCTC 690825 1.8718085 6.547982 5
TGCAG 675385 1.8676573 5.298029 3
TGTTT 1070010 1.8510897 5.7772827 3
GGCTC 514810 1.8251158 6.820912 4
GCCCA 518860 1.7797467 5.680853 4
AGGGG 483240 1.7484721 7.1361747 6
GGCCC 373060 1.6699892 6.269019 1
GCTCC 445550 1.5517101 6.7618766 2
TAAAA 1187795 1.5043046 8.541426 1
ATGGA 693970 1.4968863 9.969441 2
TGCCC 428520 1.4923999 11.208805 3
GATGG 527555 1.4850558 5.52745 1
CATGG 522140 1.4438856 40.56618 1
TGCTC 469755 1.295669 5.387774 1
ATGTG 585190 1.281596 7.522701 2
TGGGT 447825 1.2799395 6.1892548 3
ATGAA 765305 1.2648988 8.76721 2
TGAGA 575995 1.2424154 5.738297 1
ATGGC 445550 1.2320894 10.092716 2
AGACA 590145 1.231605 5.237671 2
ATGGG 427205 1.2025727 14.393996 2
ATGCC 439880 1.1949528 11.49219 2
GAGAC 409090 1.1141881 5.7793355 2
CATGT 501500 1.0789367 31.965643 1
CATGA 502870 1.0655519 29.533949 1
ATGTT 611630 1.0421308 7.858466 2
ATGCT 475135 1.0222145 7.7080874 2
TAAAG 617245 1.0201848 6.5629106 1
ATGGT 457015 1.0008862 7.568521 2
AGACT 469695 0.99525607 5.24882 1
ATGAC 456295 0.96686214 7.4203053 2
ATGAT 572860 0.9613374 6.8706627 2
ATGTA 565510 0.9490031 9.022525 2
AGACC 354040 0.9472459 5.4923253 3
CATGC 337700 0.91737646 31.591654 1
GCGCC 203750 0.9120792 5.7455397 8
CGCTG 229435 0.813398 5.5392656 6
ATGCA 353110 0.74821925 8.478378 2
TAAGT 444080 0.745227 6.470449 1
TAACT 448840 0.7399283 5.4241233 1
TAAAC 432620 0.70242274 5.2383027 1
TGCCG 154420 0.54745317 7.131109 5
Produced by FastQC (version 0.10.0)