FastQCFastQC Report
Wed 20 Mar 2013
8016-8-50.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-50.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4651996
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11130 0.23925214037157383 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10917 0.23467346059626876 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 10530 0.22635445086367229 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10525 0.2262469701177731 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10205 0.21936820238022559 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10077 0.2166166952852066 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 9070 0.19497007306111183 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 9042 0.19436818088407642 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 8289 0.17818158055165997 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 8161 0.17543007345664097 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 8108 0.17429077755010966 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8072 0.17351691617963558 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 7982 0.17158226275345034 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7942 0.1707224167862569 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7738 0.16633720235357038 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7552 0.1623389186061209 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7525 0.16175852257826534 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 7369 0.15840512330621093 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7319 0.15733031584721913 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 7233 0.15548164701775324 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 7233 0.15548164701775324 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 6993 0.1503225712145926 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6905 0.14843091008676706 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 6836 0.14694767579335838 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 6807 0.14632428746714315 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6792 0.1460018452294456 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6771 0.14555042609666904 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 6333 0.13613511275590093 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 6311 0.1356621974739445 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6302 0.135468732131326 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 6273 0.13484534380511073 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6194 0.1331471480199037 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6142 0.13202934826255225 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6039 0.12981524489702914 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6031 0.12964327570359047 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5847 0.12568798425450065 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 5838 0.12549451891188212 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 5834 0.12540853431516277 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 5800 0.12467766524304835 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 5723 0.123022461756201 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 5712 0.1227860041152228 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 5680 0.12209812734146805 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5627 0.12095883143493674 No Hit
TGAGACCGCACCAGCGACACA 5604 0.12046442000380052 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5584 0.1200344970202038 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5563 0.11958307788742725 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 5559 0.11949709329070791 No Hit
TGTGTCGCTGGTGCGGTCTCA 5540 0.11908866645629103 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5455 0.11726149377600498 No Hit
TAGTTCCACGCCTCTCCCACCTCCCTCCGCACACACCCCACCCCAGCCTG 5294 0.11380061375805138 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5229 0.11240336406136205 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5224 0.11229588331546286 No Hit
GCTTTCTGGTGTGACTCGGGGTGGGGGCTCCCACTGGTCACCTGGTGACC 5219 0.1121884025695637 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 5142 0.11053319908271632 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 5096 0.10954437622044387 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 5082 0.10924343013192617 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 4992 0.10730877670574093 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 4986 0.10717979981066192 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 4962 0.10666389223034586 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 4900 0.10533113098119604 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 4876 0.10481522340087997 No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC 4855 0.10436380426810342 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 4853 0.10432081196974374 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 4821 0.103632935195989 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 4812 0.10343946985337048 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 4754 0.10219269320093999 No Hit
CATGGAGGAAGTAGGCAGCTGACAGAAAGTGCTGCAATTGCCTGTGTCAA 4722 0.10150481642718523 No Hit
GACTGAACAAGTAGGAAAATGACAGAGTTATCTTCCCAATTGATGTAAGT 4711 0.10126835878620703 No Hit
AGAAGAGGGCTCCTTTTGTGCATATCAAACCTTGTTCCAGAATGGAGTGG 4682 0.1006449704599918 No Hit
GACCCCCTCCACTAACTCCCGAGGACGTTGGCTTTGCATCTGGTTTTTCT 4666 0.10030103207311443 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2616900 3.4590359 6.657265 2
TTTTT 2394065 3.4085433 4.181844 95-96
GGGGG 663420 3.0108962 8.353388 4
GGAGG 772645 2.739929 5.0592265 4
CCAGG 790865 2.6950076 5.5762253 5
CCAGC 785240 2.623066 8.175132 5
CCTCC 785075 2.6092763 10.534704 6
CCTGG 744955 2.5765624 5.5204268 9
CCCCA 785505 2.572201 5.4379225 1
CAGCC 758255 2.5329237 6.4217076 6
TGGGG 668320 2.4054525 10.54086 3
CTCCC 701800 2.3325033 5.044967 3
GGCAG 629665 2.1888604 5.061668 8
GCTGG 620285 2.1885312 5.8246264 7
GAGGG 598225 2.1214063 6.5616236 9
GGGGA 598160 2.1211762 5.206666 7
CCCTC 610160 2.0279286 5.525799 5
CTCCT 754030 2.0274656 6.012866 4
CTCCA 759440 2.0118952 5.618687 6
AAGAA 1175270 1.9881698 5.0176806 2
TCCAG 721340 1.949408 6.5655437 7
GCCAG 571180 1.9463935 7.620339 4
CCCAC 585680 1.9178575 6.4485226 9
TGGCT 683095 1.9113828 6.864902 3
TGCAG 673845 1.8576916 5.471589 3
TGTTT 1034320 1.8568747 5.8846984 3
GGCTC 535405 1.8517956 7.4341917 1
TCCTC 688195 1.8504459 6.914025 5
CACCA 704120 1.8378314 5.0408955 8
GCCCA 540675 1.8061054 5.824636 4
AGGGG 496480 1.7606015 7.1624928 6
AAACA 1057800 1.7541572 5.2469974 2
CCAGA 643575 1.713598 5.2944007 8
GGCCC 382200 1.6339748 5.158043 1
GCTCC 467370 1.5846034 8.046154 2
TGCCC 451930 1.5322546 11.797823 3
TAAAA 1130875 1.5171746 8.441819 1
ATGGA 670660 1.4737339 10.587123 2
GATGG 521515 1.4666661 6.1633797 1
CATGG 521795 1.4385122 41.90943 1
GCAGT 489585 1.3497138 5.0432367 4
GGGGT 373195 1.3432231 5.094515 4
TGCTC 470050 1.2893174 5.680003 1
TGGGT 449040 1.2817475 5.8590126 3
ATGTG 566430 1.2633271 7.948303 2
ATGAA 735280 1.2624713 9.360356 2
TGAGA 557960 1.2260827 5.2520432 1
AGACA 576930 1.2244356 5.162785 2
ATGGG 434555 1.222107 14.769632 2
ATGGC 438740 1.2095417 11.073571 2
ATGCC 442865 1.1968347 11.316802 2
GTCTT 516320 1.1457521 5.1678815 9
GAGAC 407760 1.1075549 5.3820343 2
CATGT 494500 1.0811477 33.220245 1
CATGA 490985 1.0576305 30.344149 1
TAAAG 601815 1.0333127 6.7725863 1
ATGTT 583980 1.0329343 8.103042 2
AGACT 460760 0.9925229 5.6668363 1
ATGCT 451330 0.9867633 7.682065 2
ATGGT 437925 0.97671837 7.70615 2
ATGAC 450775 0.97101426 7.1705675 2
AGACC 361105 0.9614867 5.1399584 3
CACTC 362465 0.9602361 5.4808993 1
ATGAT 550530 0.95940673 7.0741806 2
ATGTA 531790 0.92674863 9.4784565 2
GCGCC 207365 0.8865232 5.616517 8
CATGC 326230 0.88163066 31.093683 1
TAACT 440555 0.7526119 5.715442 1
ATGCA 347730 0.749045 8.785533 2
TAAGT 429800 0.749011 6.7385287 1
TAAAC 417190 0.70218545 5.8068733 1
TAAGG 295970 0.6503757 5.269532 1
TGCCG 162965 0.5636441 8.352711 5
CCGTC 135470 0.45930687 5.8447757 7
GCCGT 124230 0.429672 5.9170313 6
Produced by FastQC (version 0.10.0)