FastQCFastQC Report
Wed 20 Mar 2013
8016-8-51.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-51.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5868192
Filtered Sequences 0
Sequence length 1-100
%GC 45

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 16955 0.28893055987261496 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 16807 0.28640848833848653 No Hit
TGAGACCGCACCAGCGACACA 12231 0.2084287630670571 No Hit
TGTGTCGCTGGTGCGGTCTCA 12040 0.20517392750612115 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 11977 0.20410034300172866 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11890 0.20261777392423425 No Hit
* 11329 0.19305775952797727 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 11028 0.18792841134032426 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 11015 0.18770687802989405 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 10703 0.18239007857956932 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 10576 0.1802258685469051 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10550 0.17978280192604468 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10504 0.1789989148275994 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 10242 0.17453416657123694 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 10215 0.1740740589264973 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 10167 0.17325608978029347 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 10049 0.17124524896254245 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 9326 0.15892458869784765 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 9233 0.15733977347707775 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 9210 0.1569478299278551 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 9208 0.15691374788009663 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 9126 0.15551638392199846 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 9055 0.15430647122657198 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 8766 0.14938161532546992 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 8661 0.1475923078181491 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 8594 0.14645055921823963 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 8570 0.1460415746451377 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 8561 0.14588820543022452 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 8330 0.1419517289141187 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 8065 0.1374358575861185 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 8033 0.13689054482198265 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7833 0.13348234004613346 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 7800 0.13291998625811835 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7754 0.13213609915967303 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 7720 0.13155670434777866 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 7713 0.13143741718062396 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 7699 0.1311988428463145 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7688 0.1310113915836428 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7661 0.13055128393890317 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7626 0.12995484810312954 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 7521 0.12816554059580873 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 7510 0.127978089333137 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 7490 0.1276372688555521 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 7460 0.12712603813917472 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7142 0.12170699254557452 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 6996 0.11921900305920462 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6978 0.11891226462937819 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6532 0.11131196797923448 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6459 0.11006797323604954 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6441 0.10976123480622312 No Hit
CCGCGGCGCAGATCGAAACAGATCGGGCGGCTCGGGTTACACACGCACGC 6342 0.10807417344217776 No Hit
TGCTCTGTGGTCCGAGGCGAGCCGTGAAGTTGCGTGTGCGTGGCAGTGTG 6324 0.10776743501235134 No Hit
CGAGGCGAGCCGTGAAGTTGCGTGTGCGTGGCAGTGTGCGTGGCAGGATG 6316 0.10763110682131738 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 6231 0.10618261979158147 No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC 6146 0.10473413276184557 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6139 0.10461484559469082 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 6138 0.10459780457081158 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 6136 0.1045637225230531 No Hit
GAGGCACGAACACCCACGAGCGCCGCGTAACCGCAGCAGGTGGAGCGGGC 6120 0.10429106614098516 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 6092 0.10381391747236628 No Hit
GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG 6049 0.1030811534455587 No Hit
AGGGTCAGCCCACACCACCCAGCCCCCACGCCCAACCCTCGTGCCTCCCT 6007 0.10236543044263037 No Hit
CATGGGGGCCAGTTGTGGGTGGTGGCCCAGGTGCAGGAGAGGCGGGCAGT 5983 0.10195644586952846 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 5960 0.10156450232030581 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 5911 0.10072949215022275 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3167880 3.6430993 7.0219746 2
TTTTT 2883105 3.5740917 4.5037775 60-64
GGGGG 923475 3.0040896 10.688444 4
GGAGG 1036600 2.7389357 5.450086 4
CCAGC 1078200 2.6567464 8.215815 5
CCTCC 1066505 2.6063192 9.437046 6
CCCCA 1062600 2.5580792 5.9078107 1
CCTGG 992820 2.541842 5.722923 9
CAGCC 1031100 2.5406892 6.748696 6
TGGGG 896055 2.4033992 12.842289 3
CAGCA 1148410 2.3525414 5.115306 1
GGGAG 888575 2.3478196 5.8875365 6
CTCCC 941520 2.3008816 5.008587 3
GCTGG 836625 2.192378 7.59905 7
GAGGG 796095 2.1034663 5.754124 9
GGGGA 793560 2.0967681 6.5542417 5
CTCCT 977745 2.0165105 6.742655 4
AAGAA 1418605 2.0085416 5.00994 2
CTCCA 977895 1.9867649 6.195839 6
TCCAG 933515 1.9412534 6.4983873 7
TGTTT 1278325 1.921954 5.4880266 3
CCCAC 785485 1.8909588 5.8319526 9
TGGCT 865685 1.8704581 7.786717 3
GGCTC 728925 1.8662115 8.475394 1
CCCTG 744485 1.8622043 5.2925544 1
GCCAG 735465 1.8548952 6.486564 4
TCCTC 895545 1.8469803 7.4248905 5
CACCA 922715 1.8467208 5.045979 8
GCCCA 747640 1.8422276 5.968302 4
TGGTG 827265 1.8295293 5.565579 9
TGCAG 858285 1.8268338 5.304723 3
AGCTG 811680 1.7276365 5.1652555 9
CAGCT 813315 1.6912963 5.0978985 8
AGGGG 639445 1.6895609 7.0957117 6
GGCCC 527795 1.6011541 5.4044337 1
GCTCC 640090 1.6010778 8.798201 2
TAAAA 1354360 1.5810913 8.569501 1
TGCCC 612200 1.5313156 12.109757 3
GATGG 697120 1.518734 6.3571215 1
CCTCA 737470 1.4982995 5.1603465 6
ACAGC 725120 1.4854232 5.2989893 7
ATGGA 833775 1.4753869 10.089714 2
CATGG 680275 1.4479449 40.97157 1
CAACA 802645 1.3355085 5.19739 5
GCAGT 611610 1.3017935 5.455325 4
TGCTC 612205 1.2923447 6.373076 1
TGGGT 584035 1.2916166 5.9972034 3
ATGTG 706690 1.2694237 7.6279945 2
ATGGG 581040 1.265844 15.226071 2
AACTG 725760 1.2547103 5.1358056 4
AGACA 728860 1.241292 5.1803274 2
TGAGA 697525 1.2342889 6.244356 1
ATGGC 577210 1.2285742 11.787981 2
ATGAA 853715 1.2270235 8.212047 2
CTCAC 595300 1.2094563 5.374273 7
ATGCC 568715 1.1826483 12.171347 2
GGAGT 534530 1.1645181 5.9382544 7
GAGAC 533725 1.1190886 6.5808353 2
CATGT 616725 1.0823371 31.486254 1
ATGTT 720425 1.0670135 7.979588 2
CATGA 605280 1.0464218 26.27635 1
TAAAG 723015 1.0391716 7.058308 1
GTGTC 478060 1.0329291 5.460725 2
AGACT 574445 0.99311346 5.447388 1
ACAAC 588180 0.9786637 5.505172 4
ATGCT 556275 0.97624886 7.578107 2
CACTC 477820 0.9707749 5.505593 1
ATGGT 536625 0.9639367 7.411251 2
AGACC 469585 0.9619545 5.9300117 3
ATGAC 555690 0.9606894 6.4690657 2
ATGAT 657075 0.958684 6.0974827 2
ATGTA 639970 0.93372756 9.268365 2
CATGC 436100 0.9068741 30.88088 1
CGCTG 339750 0.8698361 5.9133 6
TAAGA 565535 0.8128295 5.137776 1
TAAGT 515305 0.7518391 6.587099 1
TAATG 511975 0.74698055 5.0953975 1
TAACT 522925 0.7454068 5.7775536 1
ATGCA 418495 0.7235036 8.534057 2
TAAAC 479445 0.67324346 5.3860636 1
TAAGG 359025 0.6353042 5.3554974 1
TGCCG 222365 0.5693042 6.8481007 5
GTCGC 138045 0.35342616 5.217424 4
Produced by FastQC (version 0.10.0)