##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-52.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4011186	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.933308752074822	24.0	24.0	31.0	23.0	31.0
2	31.004146658118128	31.0	31.0	32.0	29.0	33.0
3	32.635972812264235	33.0	33.0	34.0	29.0	35.0
4	33.84336953792629	34.0	33.0	36.0	29.0	37.0
5	35.38299200333551	36.0	35.0	37.0	33.0	37.0
6	35.719145061952574	36.0	36.0	38.0	33.0	38.0
7	35.7330529751601	37.0	35.0	37.0	34.0	37.0
8	35.58273587086287	36.0	36.0	37.0	32.0	38.0
9	35.603979893702366	37.0	36.0	37.0	31.0	38.0
10-14	35.96192199260541	37.0	36.0	37.4	32.2	38.0
15-19	36.94540058649302	38.0	37.2	38.4	32.6	39.4
20-24	36.576719501308006	37.4	36.0	38.8	32.8	39.8
25-29	36.226948518425345	37.6	36.2	38.0	32.8	38.2
30-34	36.051525729560474	37.6	35.6	38.2	32.4	38.6
35-39	36.070630892445365	37.8	36.4	38.0	31.6	38.6
40-44	36.01013678063781	37.4	35.8	38.4	31.8	38.8
45-49	35.97133877020252	37.6	35.8	38.2	31.6	38.6
50-54	36.18031860799728	37.6	36.2	38.2	33.0	39.0
55-59	35.89489457187938	37.6	35.6	38.2	32.0	38.6
60-64	35.915820283694	37.6	35.6	38.2	32.6	38.6
65-69	35.71905092115456	37.4	35.8	38.2	31.4	38.6
70-74	35.75255283843774	37.2	36.0	38.4	31.6	39.0
75-79	35.46529632905373	37.4	35.4	37.6	31.4	38.4
80-84	35.57348703717967	37.0	35.8	38.2	31.0	38.6
85-89	35.28308121744962	37.2	35.4	37.6	31.0	38.2
90-94	35.30064618717408	37.0	35.0	38.2	30.8	38.4
95-99	35.12381364607543	37.4	35.2	37.6	30.4	38.2
100	34.02777588440712	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1.0
1	3.0
2	29.0
3	130.0
4	0.0
5	17.0
6	12.0
7	76.0
8	160.0
9	11608.0
10	417.0
11	716.0
12	1047.0
13	1886.0
14	2478.0
15	3107.0
16	3815.0
17	4759.0
18	5993.0
19	7154.0
20	8097.0
21	8015.0
22	8110.0
23	9282.0
24	11293.0
25	14852.0
26	19547.0
27	26641.0
28	36185.0
29	48162.0
30	62929.0
31	82437.0
32	107188.0
33	143947.0
34	211105.0
35	379227.0
36	1136914.0
37	1653841.0
38	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.8488102310747	19.11414902598418	21.58566648947813	39.451374253462994
2	17.678816499151758	52.09477829614929	15.158040515668514	15.068364689030442
3	19.50234862003569	34.31308365408468	29.957533788920276	16.227033936959355
4	33.67758958102777	24.95563933749742	20.857927084701043	20.50884399677377
5	20.7062897999105	27.285256926848756	26.274322932256684	25.734130340984056
6	20.2813348301256	29.200223016036414	25.78307332328459	24.735368830553398
7	21.91138977119177	27.889083037305745	27.1472800976703	23.052247093832182
8	21.04861111111111	27.643752507019652	28.85444243882872	22.453193943040514
9	21.8504084566893	28.853901992957777	26.770240135178668	22.525449415174258
10-14	22.09016590719466	27.462845372569156	26.648687704576083	23.798301015660098
15-19	21.557491066557223	27.228209727333763	27.215194381061853	23.999104825047162
20-24	21.811147521179084	26.548214816045263	27.60363530199383	24.037002360781827
25-29	21.968390936605488	27.031866623063593	28.101824614889736	22.89791782544118
30-34	22.406159764143613	27.39505313213594	27.392738039424813	22.80604906429563
35-39	22.503312984947566	26.324189430775895	28.47125063878641	22.701246945490126
40-44	22.544037766216128	27.359749011363487	27.35472421383573	22.741489008584654
45-49	22.073985287515075	27.865280142860726	27.81758891592203	22.243145653702168
50-54	22.607605343898648	27.309402082625496	27.624849658784008	22.458142914691855
55-59	21.954633977905154	28.199979945356475	27.43005891832918	22.41532715840919
60-64	22.402854185489865	27.792218708127365	28.009068155515287	21.79585895086749
65-69	22.465625729479527	28.583083911529762	27.705328414670927	21.245961944319784
70-74	22.205496370363413	28.283199814574477	27.298372634666983	22.212931180395124
75-79	22.649067312312454	28.20178092695111	27.430163160270222	21.718988600466215
80-84	21.949701980890453	28.201063000193667	27.55068219608157	22.29855282283431
85-89	22.239821059805674	27.937558163240066	27.80622439431329	22.016396382640977
90-94	22.27613989867021	27.73168220567201	27.77582484985541	22.21635304580236
95-99	22.411420082844938	26.81968200047477	28.105951258468124	22.662946658212167
100	23.588662430983998	26.698708051082242	26.786969690996653	22.925659826937103
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	59.3001844845377
2	32.747181188182196
3	35.72938255699505
4	54.18643357780154
5	46.44042014089456
6	45.016703660679
7	44.96363686502395
8	43.501805054151625
9	44.37585787186356
10-14	45.88846692285476
15-19	45.55659589160438
20-24	45.84814988196091
25-29	44.86630876204667
30-34	45.212208828439245
35-39	45.204559930437696
40-44	45.285526774800786
45-49	44.31713094121724
50-54	45.0657482585905
55-59	44.369961136314345
60-64	44.198713136357355
65-69	43.71158767379931
70-74	44.41842755075854
75-79	44.36805591277867
80-84	44.24825480372476
85-89	44.25621744244665
90-94	44.49249294447257
95-99	45.074366741057105
100	46.5143222579211
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11955.0
1	11954.5
2	11959.0
3	11974.0
4	11986.0
5	11988.0
6	11993.0
7	12001.0
8	12012.5
9	12034.0
10	12061.0
11	12080.0
12	12089.0
13	12111.5
14	12153.0
15	12599.5
16	12819.5
17	12591.5
18	12893.0
19	13659.5
20	14370.0
21	15217.0
22	18306.0
23	22868.0
24	31601.5
25	41605.0
26	51129.0
27	61103.5
28	63701.5
29	75369.0
30	97028.5
31	106479.5
32	109851.5
33	120218.5
34	132687.0
35	132606.0
36	128565.5
37	123506.0
38	138210.0
39	150789.5
40	153768.5
41	165356.0
42	157520.5
43	160448.0
44	164041.5
45	151867.0
46	140517.5
47	126025.5
48	120569.5
49	127578.5
50	131792.5
51	134933.5
52	115297.0
53	77505.5
54	68567.5
55	68711.5
56	54689.0
57	45092.0
58	60444.0
59	62127.0
60	60440.0
61	72412.0
62	63380.5
63	56233.0
64	52918.5
65	49551.0
66	51120.0
67	46611.5
68	35625.0
69	30334.5
70	28463.0
71	25028.0
72	27469.0
73	20107.0
74	10705.0
75	13074.5
76	14049.5
77	13461.0
78	11543.5
79	11215.0
80	10088.5
81	8214.0
82	7340.5
83	7110.0
84	7064.5
85	6164.5
86	7174.0
87	6856.5
88	6031.0
89	6389.5
90	6916.0
91	7491.0
92	6295.5
93	5130.0
94	5109.5
95	5103.5
96	5099.0
97	5096.5
98	5101.5
99	5104.5
100	5102.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05524550594263143
2	0.006901511431003389
3	0.0016254026935173188
4	0.007027185454676404
5	0.004479713778823767
6	8.014887653816964E-4
7	3.7603927855610944E-4
8	3.5098151982017713E-4
9	1.2535098275170476E-4
10-14	0.001158248249302757
15-19	0.017150231312491156
20-24	0.015541495289135227
25-29	0.00872254581229465
30-34	0.00787473888391902
35-39	0.005672718933172588
40-44	0.012241379956530693
45-49	0.011178758230075574
50-54	0.010574845337164625
55-59	0.011368470839744215
60-64	0.0527017482982503
65-69	0.041940142471817154
70-74	0.026012450825849544
75-79	0.07372574781762287
80-84	0.06307592517854406
85-89	0.014594657118839846
90-94	0.00767453350887849
95-99	0.0100814549123281
100	0.008787064783661643
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	12062.0
2-3	368.0
4-5	6186.0
6-7	3756.0
8-9	18.0
10-11	8.0
12-13	19.0
14-15	114.0
16-17	344.0
18-19	1679.0
20-21	17318.0
22-23	2795.0
24-25	3463.0
26-27	1924.0
28-29	1759.0
30-31	2965.0
32-33	1466.0
34-35	2153.0
36-37	1098.0
38-39	913.0
40-41	2263.0
42-43	1177.0
44-45	1560.0
46-47	1841.0
48-49	935.0
50-51	14130.0
52-53	1804.0
54-55	15376.0
56-57	11021.0
58-59	30851.0
60-61	1892.0
62-63	10397.0
64-65	4338.0
66-67	5436.0
68-69	5487.0
70-71	19050.0
72-73	19181.0
74-75	8528.0
76-77	12462.0
78-79	4569.0
80-81	8858.0
82-83	9173.0
84-85	6985.0
86-87	1845.0
88-89	3750.0
90-91	8541.0
92-93	1070.0
94-95	2529.0
96-97	82632.0
98-99	0.0
100-101	3653097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.05082090475031
#Duplication Level	Relative count
1	100.0
2	6.052562617817865
3	1.858035284073138
4	1.0515904883471263
5	0.8529379443265679
6	0.8258873851407897
7	0.8208154052934563
8	0.9222550022401245
9	0.9222550022401245
10++	12.054405437162396
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	11345	0.28283405456640504	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	9414	0.23469367912632325	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	9404	0.2344443763016724	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	8510	0.21215670377788517	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	8445	0.21053623541765454	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	8069	0.20116244921078208	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	8063	0.20101286751599154	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	7253	0.18081933871927156	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	7245	0.18061989645955087	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	6315	0.15743473376702052	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	6274	0.156412592185952	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	6197	0.15449296043614033	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	6196	0.15446803015367525	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	6145	0.15319658574795583	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	5980	0.1490830891412166	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	5958	0.1485346229269847	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	5905	0.1472133179563351	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	5742	0.14314968191452604	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	5693	0.1419280980737368	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	5671	0.1413796318595049	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	5627	0.1402826994310411	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	5509	0.13734092610016088	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	5423	0.13519692180816348	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	5341	0.1331526386460264	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	5333	0.1329531963863057	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	5323	0.13270389356165482	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	5236	0.1305349589871923	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	5230	0.1303853772924018	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	5179	0.12911393288668238	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	5158	0.12859039695491556	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	5142	0.1281915124354742	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	5121	0.12766797650370737	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	5116	0.12754332509138194	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	5109	0.12736881311412634	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	5095	0.12701978915961512	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	4945	0.1232802467898522	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	4928	0.1228564319879457	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	4883	0.12173456927701681	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	4872	0.12146033616990087	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	4834	0.12051298543622758	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	4804	0.11976507696227501	No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT	4780	0.11916675018311292	No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA	4772	0.11896730792339225	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	4750	0.11841884170916032	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	4587	0.11435520566735126	No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC	4515	0.11256022532986504	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	4429	0.11041622103786759	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	4423	0.11026663934307708	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	4399	0.10966831256391502	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	4381	0.10921956747954346	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	4351	0.10847165900559087	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	4339	0.10817249561600982	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	4266	0.10635258499605853	No Hit
TGAGACCGCACCAGCGACACA	4224	0.10530551313252488	No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG	4215	0.10508114059033913	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	4192	0.10450774409364212	No Hit
TGTGTCGCTGGTGCGGTCTCA	4167	0.10388448703201496	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	4159	0.10368504477229429	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	4155	0.10358532364243393	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	4126	0.10286234545094644	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	4101	0.10223908838931928	No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA	4041	0.10074327144141408	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2231215	3.6065288	6.661917	2
TTTTT	1970390	3.4560256	4.192566	75-79
GGGGG	613520	3.0491023	8.345259	4
GGAGG	682200	2.7082946	5.4383435	4
CCTCC	701185	2.6144493	9.002428	6
CCAGC	694295	2.597642	7.5059075	5
CCCCA	706210	2.5905147	5.7999215	1
CAGCC	687790	2.573304	5.778667	6
CCTGG	660930	2.5637107	5.0471134	9
TGGGG	591830	2.3882322	10.423905	3
CTCCC	616470	2.2985795	5.361922	3
GCTGG	551385	2.1814814	5.524743	7
GAGGG	535780	2.127016	6.3136153	9
GGGGA	523760	2.0792973	5.48435	7
CTCCT	658230	2.0325654	5.9512644	4
CTCCA	647510	1.967062	5.6458054	6
CCCAC	524430	1.9237106	5.796023	9
TCCAG	618990	1.917954	5.9220715	7
GCCAG	498490	1.902281	6.5112967	4
TGGCT	590610	1.8972898	6.9849544	3
TGTTT	874365	1.8887794	5.607316	3
TGCAG	591295	1.8687093	5.4469137	3
TCCTC	599045	1.8498065	6.8599873	5
CACCA	612000	1.8290584	5.1479974	8
GGCTC	470410	1.8246942	8.138913	1
GCCCA	486000	1.8183249	5.5622926	4
AAACA	901790	1.7890788	5.166782	2
AGGGG	432015	1.7150749	7.058991	6
GGCCC	345690	1.6191274	5.8996153	1
GCTCC	419065	1.5937197	8.416669	2
TAAAA	952580	1.56511	8.398525	1
TGCCC	401105	1.5254172	11.511789	3
GATGG	468205	1.5092341	5.9376454	1
ATGGA	567010	1.459998	10.738587	2
CATGG	454030	1.4349015	41.151333	1
TGGGT	390445	1.2793099	5.957897	3
ATGTG	488010	1.2772784	7.600755	2
TGCTC	405445	1.2769732	5.6560273	1
ATGGG	383760	1.23703	14.704278	2
ATGAA	600655	1.2354568	8.08356	2
ATGGC	390755	1.2349293	11.215151	2
AGACA	495145	1.2297463	5.2368836	2
GGCAC	318950	1.2171407	5.084531	1
ATGCC	381310	1.1814972	11.998049	2
ATGTT	504610	1.0723807	7.71516	2
CATGT	413885	1.0620708	31.630182	1
CATGA	412845	1.0422347	27.503017	1
TAAAG	505965	1.0406938	6.873892	1
ATGCT	388210	0.99618626	8.090486	2
ATGAT	471370	0.9855071	6.4863124	2
AGACT	388650	0.9811539	5.0778785	1
ATGGT	372760	0.97563225	7.257432	2
ATGAC	378620	0.9558331	6.778554	2
ATGAG	369435	0.95126075	5.044756	2
ATGTA	444015	0.92831516	9.12827	2
CATGC	295700	0.9162328	32.116608	1
TAAGA	397440	0.8174741	5.0558286	1
TAAGT	362240	0.7573458	6.6143937	1
ATGCA	299195	0.75532323	9.282801	2
TAACT	366090	0.7504169	5.6192102	1
ATGTC	283895	0.72850335	5.0945864	2
TAAAC	337630	0.68086416	5.521564	1
TAAGG	245530	0.6322169	5.3630886	1
TGCCG	149965	0.5817059	7.827133	5
CCGTC	124360	0.4729457	5.143618	7
GCCGT	117060	0.45406926	5.204603	6
>>END_MODULE