FastQCFastQC Report
Wed 20 Mar 2013
8016-8-52.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-52.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4011186
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 11345 0.28283405456640504 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 9414 0.23469367912632325 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 9404 0.2344443763016724 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8510 0.21215670377788517 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8445 0.21053623541765454 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 8069 0.20116244921078208 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 8063 0.20101286751599154 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 7253 0.18081933871927156 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7245 0.18061989645955087 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 6315 0.15743473376702052 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 6274 0.156412592185952 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 6197 0.15449296043614033 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 6196 0.15446803015367525 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6145 0.15319658574795583 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 5980 0.1490830891412166 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 5958 0.1485346229269847 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 5905 0.1472133179563351 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 5742 0.14314968191452604 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 5693 0.1419280980737368 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 5671 0.1413796318595049 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 5627 0.1402826994310411 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 5509 0.13734092610016088 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 5423 0.13519692180816348 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 5341 0.1331526386460264 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 5333 0.1329531963863057 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 5323 0.13270389356165482 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 5236 0.1305349589871923 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 5230 0.1303853772924018 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 5179 0.12911393288668238 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5158 0.12859039695491556 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 5142 0.1281915124354742 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 5121 0.12766797650370737 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 5116 0.12754332509138194 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 5109 0.12736881311412634 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 5095 0.12701978915961512 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 4945 0.1232802467898522 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 4928 0.1228564319879457 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 4883 0.12173456927701681 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 4872 0.12146033616990087 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 4834 0.12051298543622758 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 4804 0.11976507696227501 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 4780 0.11916675018311292 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 4772 0.11896730792339225 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 4750 0.11841884170916032 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 4587 0.11435520566735126 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 4515 0.11256022532986504 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 4429 0.11041622103786759 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 4423 0.11026663934307708 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 4399 0.10966831256391502 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 4381 0.10921956747954346 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 4351 0.10847165900559087 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4339 0.10817249561600982 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 4266 0.10635258499605853 No Hit
TGAGACCGCACCAGCGACACA 4224 0.10530551313252488 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 4215 0.10508114059033913 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 4192 0.10450774409364212 No Hit
TGTGTCGCTGGTGCGGTCTCA 4167 0.10388448703201496 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 4159 0.10368504477229429 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 4155 0.10358532364243393 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 4126 0.10286234545094644 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 4101 0.10223908838931928 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 4041 0.10074327144141408 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2231215 3.6065288 6.661917 2
TTTTT 1970390 3.4560256 4.192566 75-79
GGGGG 613520 3.0491023 8.345259 4
GGAGG 682200 2.7082946 5.4383435 4
CCTCC 701185 2.6144493 9.002428 6
CCAGC 694295 2.597642 7.5059075 5
CCCCA 706210 2.5905147 5.7999215 1
CAGCC 687790 2.573304 5.778667 6
CCTGG 660930 2.5637107 5.0471134 9
TGGGG 591830 2.3882322 10.423905 3
CTCCC 616470 2.2985795 5.361922 3
GCTGG 551385 2.1814814 5.524743 7
GAGGG 535780 2.127016 6.3136153 9
GGGGA 523760 2.0792973 5.48435 7
CTCCT 658230 2.0325654 5.9512644 4
CTCCA 647510 1.967062 5.6458054 6
CCCAC 524430 1.9237106 5.796023 9
TCCAG 618990 1.917954 5.9220715 7
GCCAG 498490 1.902281 6.5112967 4
TGGCT 590610 1.8972898 6.9849544 3
TGTTT 874365 1.8887794 5.607316 3
TGCAG 591295 1.8687093 5.4469137 3
TCCTC 599045 1.8498065 6.8599873 5
CACCA 612000 1.8290584 5.1479974 8
GGCTC 470410 1.8246942 8.138913 1
GCCCA 486000 1.8183249 5.5622926 4
AAACA 901790 1.7890788 5.166782 2
AGGGG 432015 1.7150749 7.058991 6
GGCCC 345690 1.6191274 5.8996153 1
GCTCC 419065 1.5937197 8.416669 2
TAAAA 952580 1.56511 8.398525 1
TGCCC 401105 1.5254172 11.511789 3
GATGG 468205 1.5092341 5.9376454 1
ATGGA 567010 1.459998 10.738587 2
CATGG 454030 1.4349015 41.151333 1
TGGGT 390445 1.2793099 5.957897 3
ATGTG 488010 1.2772784 7.600755 2
TGCTC 405445 1.2769732 5.6560273 1
ATGGG 383760 1.23703 14.704278 2
ATGAA 600655 1.2354568 8.08356 2
ATGGC 390755 1.2349293 11.215151 2
AGACA 495145 1.2297463 5.2368836 2
GGCAC 318950 1.2171407 5.084531 1
ATGCC 381310 1.1814972 11.998049 2
ATGTT 504610 1.0723807 7.71516 2
CATGT 413885 1.0620708 31.630182 1
CATGA 412845 1.0422347 27.503017 1
TAAAG 505965 1.0406938 6.873892 1
ATGCT 388210 0.99618626 8.090486 2
ATGAT 471370 0.9855071 6.4863124 2
AGACT 388650 0.9811539 5.0778785 1
ATGGT 372760 0.97563225 7.257432 2
ATGAC 378620 0.9558331 6.778554 2
ATGAG 369435 0.95126075 5.044756 2
ATGTA 444015 0.92831516 9.12827 2
CATGC 295700 0.9162328 32.116608 1
TAAGA 397440 0.8174741 5.0558286 1
TAAGT 362240 0.7573458 6.6143937 1
ATGCA 299195 0.75532323 9.282801 2
TAACT 366090 0.7504169 5.6192102 1
ATGTC 283895 0.72850335 5.0945864 2
TAAAC 337630 0.68086416 5.521564 1
TAAGG 245530 0.6322169 5.3630886 1
TGCCG 149965 0.5817059 7.827133 5
CCGTC 124360 0.4729457 5.143618 7
GCCGT 117060 0.45406926 5.204603 6
Produced by FastQC (version 0.10.0)