##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-53.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6493068	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.93035880726954	24.0	24.0	31.0	23.0	31.0
2	31.00099858835708	31.0	31.0	32.0	27.0	33.0
3	32.63731868806549	33.0	33.0	34.0	29.0	35.0
4	33.842641714260566	34.0	33.0	36.0	29.0	37.0
5	35.38302510604461	36.0	35.0	37.0	33.0	37.0
6	35.71324108472984	36.0	36.0	38.0	33.0	38.0
7	35.73192037571392	37.0	35.0	37.0	34.0	37.0
8	35.58012647153509	36.0	36.0	37.0	32.0	38.0
9	35.59490487540052	37.0	36.0	37.0	31.0	38.0
10-14	35.95992500575165	37.0	36.0	37.4	32.2	38.0
15-19	36.94680619626328	38.0	37.2	38.4	32.6	39.4
20-24	36.58509220007709	37.4	36.0	38.8	32.8	39.8
25-29	36.23875537848329	37.6	36.2	38.0	32.8	38.2
30-34	36.071376075880806	37.6	35.6	38.2	32.4	38.6
35-39	36.10220170320065	37.8	36.4	38.0	31.6	38.6
40-44	36.03572241612781	37.4	35.8	38.4	31.8	38.8
45-49	35.98272416895647	37.6	35.8	38.2	31.8	38.6
50-54	36.195678455443854	37.6	36.2	38.2	33.0	39.0
55-59	35.9180360907675	37.6	35.8	38.2	32.4	38.6
60-64	35.93531527323602	37.6	35.6	38.2	32.6	38.6
65-69	35.73719421477835	37.4	36.0	38.2	31.4	38.6
70-74	35.77584710457408	37.4	36.0	38.4	31.6	39.0
75-79	35.48354634107328	37.4	35.4	37.6	31.6	38.4
80-84	35.59631717516552	37.0	35.8	38.2	31.0	38.4
85-89	35.30670224409706	37.2	35.4	37.6	31.0	38.2
90-94	35.320268360363855	37.0	35.0	38.2	30.8	38.4
95-99	35.15090807712373	37.4	35.2	37.6	30.8	38.2
100	34.0846378907708	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1.0
1	9.0
2	46.0
3	211.0
4	2.0
5	20.0
6	37.0
7	169.0
8	282.0
9	16255.0
10	736.0
11	1169.0
12	1749.0
13	3195.0
14	4132.0
15	5292.0
16	6130.0
17	7339.0
18	8908.0
19	10613.0
20	11791.0
21	12074.0
22	12684.0
23	14508.0
24	17715.0
25	23227.0
26	30885.0
27	42971.0
28	58715.0
29	77434.0
30	100992.0
31	131582.0
32	171400.0
33	230437.0
34	341313.0
35	612305.0
36	1848732.0
37	2687998.0
38	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.228221695108367	19.815663931757225	22.03669915234078	38.91941522079364
2	16.90696053326893	53.07224783428216	15.529592095473497	14.49119953697541
3	18.89423230599821	35.45772382166998	30.385024060818903	15.263019811512905
4	32.99981112934613	25.480241859450892	21.57149958820638	19.9484474229966
5	20.079716519540728	27.9934327109333	27.224910956595146	24.701939812930828
6	19.993172478070566	30.02724981760564	26.527797116585116	23.45178058773868
7	21.525728199774377	28.638617719239218	27.98666121578562	21.848992865200785
8	20.77630919141881	28.1392135133242	29.47745529491279	21.607022000344198
9	21.23147247163031	29.07440173886663	27.45237081698868	22.241754972514386
10-14	21.518533821309084	28.02902371242996	27.36720092877859	23.085241537482364
15-19	20.951936091495572	27.889779806854893	27.92843444359226	23.229849658057276
20-24	21.219243171628356	27.188419846329744	28.193873883433273	23.39846309860863
25-29	21.405974785527704	27.532592683118377	28.72773171710264	22.33370081425128
30-34	22.069594234083347	27.82207764544829	28.012082558809333	22.096245561659032
35-39	22.009545709148277	26.80850122216874	29.090209532519793	22.09174353616319
40-44	22.109934898952964	27.861295817271003	27.7321776473598	22.296591636416228
45-49	21.770154479459777	28.446285207811645	28.373105412200488	21.41045490052809
50-54	22.10141137636679	27.85829284335337	28.095049175279698	21.94524660500014
55-59	21.455564075895207	28.807706623512864	27.822659772197305	21.914069528394617
60-64	21.904413102851244	28.26675787017297	28.58622445479564	21.242604572180145
65-69	21.868886657555883	29.06076987353173	28.275141748253716	20.79520172065867
70-74	21.728305307085535	28.668293817782075	27.821666853895216	21.781734021237174
75-79	22.303245469506123	28.693285474997136	27.81049742258209	21.19297163291465
80-84	21.506529203314447	28.646772403252562	28.122577698428998	21.724120695003993
85-89	21.714056127800436	28.381897440496346	28.368926293200342	21.53512013850288
90-94	21.612779074157462	28.076038635162575	28.422560380191857	21.888621910488105
95-99	21.80038992637419	27.46753102283817	28.64011289571811	22.091966155069528
100	22.999253936294046	27.5449969143861	27.15433298198905	22.301416167330803
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	58.147636915902
2	31.39816007024434
3	34.15725211751111
4	52.94825855234273
5	44.781656332471556
6	43.444953065809244
7	43.37472106497516
8	42.38333119176301
9	43.47322744414469
10-14	44.603775358791445
15-19	44.18178574955285
20-24	44.61770627023699
25-29	43.739675599778984
30-34	44.16583979574238
35-39	44.10128924531146
40-44	44.406526535369196
45-49	43.18060937998787
50-54	44.046657981366934
55-59	43.369633604289824
60-64	43.14701767503139
65-69	42.66408837821455
70-74	43.510039328322705
75-79	43.49621710242077
80-84	43.23064989831844
85-89	43.249176266303316
90-94	43.501400984645564
95-99	43.892356081443715
100	45.300670103624846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16728.0
1	16725.5
2	16727.5
3	16732.0
4	16736.0
5	16746.0
6	16757.5
7	16796.0
8	16835.5
9	16859.0
10	16890.0
11	16929.5
12	16954.5
13	17038.5
14	17104.5
15	17676.5
16	17985.5
17	17894.5
18	18360.0
19	19240.0
20	20702.0
21	22976.0
22	28609.5
23	37121.0
24	52970.5
25	69030.5
26	89068.0
27	110825.5
28	109661.5
29	124099.5
30	162506.0
31	182209.5
32	186795.5
33	208812.5
34	233565.5
35	229797.0
36	218621.0
37	206952.0
38	232059.0
39	254704.0
40	257675.0
41	276587.5
42	267190.0
43	268383.5
44	277609.5
45	257265.0
46	229936.5
47	200324.0
48	191254.0
49	207120.0
50	212615.5
51	212587.0
52	180238.5
53	117198.5
54	98854.0
55	98495.5
56	77813.0
57	65873.5
58	84956.5
59	83866.0
60	82475.5
61	102081.0
62	90965.5
63	80781.0
64	75560.0
65	68913.5
66	68813.5
67	61521.0
68	46440.0
69	41153.0
70	37947.0
71	31883.5
72	36999.0
73	27180.0
74	12776.0
75	15649.0
76	16704.5
77	15141.5
78	13040.5
79	12369.5
80	10927.5
81	9002.0
82	7678.0
83	7244.0
84	6956.0
85	6043.0
86	8376.5
87	7852.5
88	5635.5
89	5910.0
90	6214.0
91	6608.0
92	5494.5
93	4355.5
94	4271.5
95	4267.5
96	4257.5
97	4255.5
98	4250.0
99	4244.5
100	4244.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05276396304489649
2	0.007102994344472372
3	0.00174490587854158
4	0.0071651434302660915
5	0.004588301869478106
6	7.881414080223522E-4
7	2.783157690313096E-4
8	1.391632208306931E-4
9	1.5462637474444124E-5
10-14	0.0012463142883624276
15-19	0.017494387938918603
20-24	0.015811389840733246
25-29	0.008429056021418899
30-34	0.008069272270435362
35-39	0.005526988870248522
40-44	0.012393308388052228
45-49	0.011403342961259987
50-54	0.010763201629734386
55-59	0.010656048876755432
60-64	0.05340264127077229
65-69	0.04298324674881357
70-74	0.02715232943983254
75-79	0.07296351143439851
80-84	0.06317323627160508
85-89	0.014861935580964911
90-94	0.006915233953422417
95-99	0.009493039617841356
100	0.008375382264290517
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	16926.0
2-3	347.0
4-5	4875.0
6-7	3694.0
8-9	78.0
10-11	85.0
12-13	69.0
14-15	304.0
16-17	259.0
18-19	3610.0
20-21	31814.0
22-23	4266.0
24-25	4962.0
26-27	2458.0
28-29	1879.0
30-31	3587.0
32-33	2059.0
34-35	2297.0
36-37	1772.0
38-39	1419.0
40-41	3513.0
42-43	1707.0
44-45	1654.0
46-47	2825.0
48-49	1468.0
50-51	22286.0
52-53	3456.0
54-55	28250.0
56-57	18722.0
58-59	49251.0
60-61	2567.0
62-63	12420.0
64-65	8128.0
66-67	8668.0
68-69	11916.0
70-71	31006.0
72-73	30676.0
74-75	20051.0
76-77	21695.0
78-79	6708.0
80-81	15869.0
82-83	15274.0
84-85	9802.0
86-87	3560.0
88-89	5475.0
90-91	13989.0
92-93	4236.0
94-95	4278.0
96-97	136680.0
98-99	0.0
100-101	5910178.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.18984736166736
#Duplication Level	Relative count
1	100.0
2	6.800093202359491
3	2.0259249721006096
4	0.973106213899415
5	0.6211446721361735
6	0.5015758556835044
7	0.4341267797358449
8	0.43044773922960894
9	0.46723814429196864
10++	10.381025961762505
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	15814	0.24355204658260163	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	13782	0.21225713329969748	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	13728	0.2114254771396203	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	13222	0.2036325508988971	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	13183	0.20303191033884138	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	12319	0.18972541177760652	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	12208	0.18801589633744786	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	11387	0.1753716424962745	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	11147	0.17167539289593148	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	11093	0.1708437367358543	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	10891	0.1677327266555656	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	10827	0.16674706009547413	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	10723	0.16514535193532548	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	10669	0.16431369577524832	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	10569	0.1627735917751054	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	9830	0.1513922232140492	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	9816	0.1511766086540292	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	9726	0.14979051505390056	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	9701	0.14940548905386483	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	9691	0.14925147865385055	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	9676	0.1490204630538291	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	9496	0.14624827585357184	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	9402	0.1448005780934375	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	8761	0.13492851145252138	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	8660	0.133373006412377	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	8585	0.13221792841226981	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	8508	0.13103204833215978	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	8487	0.13070862649212978	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	8397	0.12932253289200113	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	8313	0.12802884553188107	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	8266	0.1273049966518139	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	8238	0.12687376753177387	No Hit
TGAGACCGCACCAGCGACACA	8181	0.12599590825169243	No Hit
TGTGTCGCTGGTGCGGTCTCA	8054	0.12403997617151091	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	7647	0.11777175289092923	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	7509	0.11564640937073199	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	7320	0.11273561281046186	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	7286	0.11221197745041327	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	7189	0.11071807657027463	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	7151	0.11013283705022033	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	7140	0.1099634256102046	No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA	7132	0.10984021729019318	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	7113	0.10954759753016602	No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG	7067	0.10883914969010028	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	6994	0.10771487376999594	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	6981	0.10751466024997736	No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT	6951	0.10705262904993448	No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT	6927	0.10668300408990018	No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG	6831	0.10520450424976296	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	6819	0.10501969176974583	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	6745	0.10388001480964007	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	6620	0.10195488480946142	No Hit
ATCATGTCCCCAGAAAATCCAGTTACCTCTTGGTCAGTCATCCGGAAGCA	6540	0.10072280160934707	No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG	6521	0.10043018184931991	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	6520	0.1004147808093185	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3806790	3.4544048	6.516974	2
TTTTT	3436780	3.3559246	4.1465697	60-64
GGGGG	920845	3.1723413	8.371273	4
GGAGG	1058800	2.793389	5.2403126	4
CCTCC	1054410	2.6402104	9.411015	6
CCAGC	1046735	2.6264005	8.12756	5
CCTGG	1009180	2.6129227	5.7121687	9
CCCCA	1053495	2.5995147	5.51388	1
CAGCC	1008075	2.5293972	5.865898	6
TGGGG	901875	2.414533	11.0589905	3
GCTGG	833890	2.1954882	6.6800876	7
GAGGG	812780	2.1443248	6.8764267	9
GGGGA	806875	2.1287458	5.9509296	7
CCACC	841365	2.0760806	5.1371474	1
CTCCT	1029315	2.0367382	5.2823043	4
AAGAA	1660205	1.9672151	5.051149	2
GCCAG	765825	1.9539713	7.1663227	4
TCCAG	978275	1.9397379	6.041102	7
CCCAC	782835	1.931657	6.0073195	9
TGGCT	941075	1.9254844	6.445084	3
TCCTC	944600	1.8691099	6.1248603	5
CACCA	971525	1.8668197	5.85533	8
TGCAG	919080	1.8531042	6.1262045	3
TGTTT	1471200	1.8485823	5.464967	3
GGCTC	696045	1.8021678	6.800605	4
GCCCA	711315	1.7847861	5.5029635	4
AGGGG	669650	1.7667106	7.7780876	6
CCAGA	879040	1.7175978	5.189417	8
GGCCC	494365	1.6197491	5.300637	1
GAAAG	1037430	1.6051836	5.006836	5
GCTCC	616765	1.5704105	7.10413	2
TAAAA	1639625	1.5098301	8.421245	1
TGCCC	592475	1.5085632	10.398823	3
GATGG	732830	1.5024992	5.4947467	1
ATGGA	949160	1.490303	10.245055	2
CATGG	727765	1.4673634	41.380844	1
GGGGT	497290	1.3313631	5.3189826	4
GCAGT	659610	1.3299452	5.5397434	4
TGCTC	650820	1.3095202	5.418778	1
ATGTG	815850	1.2999141	7.8199954	2
TGGGT	616870	1.2834349	5.9950976	3
ATGAA	1040370	1.2509707	8.770281	2
TGAGA	788950	1.2387528	5.3002734	1
ATGGG	600110	1.2303872	14.569524	2
ATGGC	609575	1.2290616	10.166813	2
AGACA	801420	1.2194434	5.138237	2
ATGCC	608545	1.2066318	10.58869	2
GAGAC	553540	1.0998319	5.3980193	2
CATGT	693620	1.0868298	32.597958	1
CATGA	684635	1.0571333	29.176916	1
ATGTT	836010	1.0351642	8.006532	2
TAAAG	847460	1.0190102	6.6707344	1
ATGCT	642210	1.0062758	7.2433734	2
ATGGT	623655	0.99368507	7.6072774	2
ATGAC	631085	0.9744477	7.107646	2
ATGAT	787550	0.9609631	6.62035	2
AGACC	484080	0.94586676	5.0033627	3
ATGTA	756645	0.923253	9.16585	2
CATGC	449885	0.89203846	30.633993	1
GCGCC	264325	0.8660406	5.6828265	8
ATGCA	487930	0.7534045	8.947806	2
TAAGT	605830	0.73922956	6.6023617	1
TAACT	615115	0.73810893	5.473171	1
TAAAC	582465	0.688755	5.2935586	1
TAAGG	422520	0.6634106	5.5209346	1
TGCCG	210550	0.5451464	7.556058	5
CCGTC	168405	0.42879376	5.1309104	7
GCCGT	160295	0.41502845	5.2005205	6
>>END_MODULE