FastQCFastQC Report
Wed 20 Mar 2013
8016-8-53.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-53.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6493068
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 15814 0.24355204658260163 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 13782 0.21225713329969748 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 13728 0.2114254771396203 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 13222 0.2036325508988971 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 13183 0.20303191033884138 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 12319 0.18972541177760652 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 12208 0.18801589633744786 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 11387 0.1753716424962745 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 11147 0.17167539289593148 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 11093 0.1708437367358543 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 10891 0.1677327266555656 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 10827 0.16674706009547413 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 10723 0.16514535193532548 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 10669 0.16431369577524832 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 10569 0.1627735917751054 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 9830 0.1513922232140492 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 9816 0.1511766086540292 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 9726 0.14979051505390056 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 9701 0.14940548905386483 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 9691 0.14925147865385055 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 9676 0.1490204630538291 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 9496 0.14624827585357184 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 9402 0.1448005780934375 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 8761 0.13492851145252138 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 8660 0.133373006412377 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 8585 0.13221792841226981 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 8508 0.13103204833215978 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 8487 0.13070862649212978 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 8397 0.12932253289200113 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 8313 0.12802884553188107 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 8266 0.1273049966518139 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 8238 0.12687376753177387 No Hit
TGAGACCGCACCAGCGACACA 8181 0.12599590825169243 No Hit
TGTGTCGCTGGTGCGGTCTCA 8054 0.12403997617151091 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7647 0.11777175289092923 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 7509 0.11564640937073199 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 7320 0.11273561281046186 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 7286 0.11221197745041327 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 7189 0.11071807657027463 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 7151 0.11013283705022033 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 7140 0.1099634256102046 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 7132 0.10984021729019318 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 7113 0.10954759753016602 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 7067 0.10883914969010028 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 6994 0.10771487376999594 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 6981 0.10751466024997736 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT 6951 0.10705262904993448 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 6927 0.10668300408990018 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 6831 0.10520450424976296 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6819 0.10501969176974583 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6745 0.10388001480964007 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 6620 0.10195488480946142 No Hit
ATCATGTCCCCAGAAAATCCAGTTACCTCTTGGTCAGTCATCCGGAAGCA 6540 0.10072280160934707 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 6521 0.10043018184931991 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 6520 0.1004147808093185 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3806790 3.4544048 6.516974 2
TTTTT 3436780 3.3559246 4.1465697 60-64
GGGGG 920845 3.1723413 8.371273 4
GGAGG 1058800 2.793389 5.2403126 4
CCTCC 1054410 2.6402104 9.411015 6
CCAGC 1046735 2.6264005 8.12756 5
CCTGG 1009180 2.6129227 5.7121687 9
CCCCA 1053495 2.5995147 5.51388 1
CAGCC 1008075 2.5293972 5.865898 6
TGGGG 901875 2.414533 11.0589905 3
GCTGG 833890 2.1954882 6.6800876 7
GAGGG 812780 2.1443248 6.8764267 9
GGGGA 806875 2.1287458 5.9509296 7
CCACC 841365 2.0760806 5.1371474 1
CTCCT 1029315 2.0367382 5.2823043 4
AAGAA 1660205 1.9672151 5.051149 2
GCCAG 765825 1.9539713 7.1663227 4
TCCAG 978275 1.9397379 6.041102 7
CCCAC 782835 1.931657 6.0073195 9
TGGCT 941075 1.9254844 6.445084 3
TCCTC 944600 1.8691099 6.1248603 5
CACCA 971525 1.8668197 5.85533 8
TGCAG 919080 1.8531042 6.1262045 3
TGTTT 1471200 1.8485823 5.464967 3
GGCTC 696045 1.8021678 6.800605 4
GCCCA 711315 1.7847861 5.5029635 4
AGGGG 669650 1.7667106 7.7780876 6
CCAGA 879040 1.7175978 5.189417 8
GGCCC 494365 1.6197491 5.300637 1
GAAAG 1037430 1.6051836 5.006836 5
GCTCC 616765 1.5704105 7.10413 2
TAAAA 1639625 1.5098301 8.421245 1
TGCCC 592475 1.5085632 10.398823 3
GATGG 732830 1.5024992 5.4947467 1
ATGGA 949160 1.490303 10.245055 2
CATGG 727765 1.4673634 41.380844 1
GGGGT 497290 1.3313631 5.3189826 4
GCAGT 659610 1.3299452 5.5397434 4
TGCTC 650820 1.3095202 5.418778 1
ATGTG 815850 1.2999141 7.8199954 2
TGGGT 616870 1.2834349 5.9950976 3
ATGAA 1040370 1.2509707 8.770281 2
TGAGA 788950 1.2387528 5.3002734 1
ATGGG 600110 1.2303872 14.569524 2
ATGGC 609575 1.2290616 10.166813 2
AGACA 801420 1.2194434 5.138237 2
ATGCC 608545 1.2066318 10.58869 2
GAGAC 553540 1.0998319 5.3980193 2
CATGT 693620 1.0868298 32.597958 1
CATGA 684635 1.0571333 29.176916 1
ATGTT 836010 1.0351642 8.006532 2
TAAAG 847460 1.0190102 6.6707344 1
ATGCT 642210 1.0062758 7.2433734 2
ATGGT 623655 0.99368507 7.6072774 2
ATGAC 631085 0.9744477 7.107646 2
ATGAT 787550 0.9609631 6.62035 2
AGACC 484080 0.94586676 5.0033627 3
ATGTA 756645 0.923253 9.16585 2
CATGC 449885 0.89203846 30.633993 1
GCGCC 264325 0.8660406 5.6828265 8
ATGCA 487930 0.7534045 8.947806 2
TAAGT 605830 0.73922956 6.6023617 1
TAACT 615115 0.73810893 5.473171 1
TAAAC 582465 0.688755 5.2935586 1
TAAGG 422520 0.6634106 5.5209346 1
TGCCG 210550 0.5451464 7.556058 5
CCGTC 168405 0.42879376 5.1309104 7
GCCGT 160295 0.41502845 5.2005205 6
Produced by FastQC (version 0.10.0)