FastQCFastQC Report
Wed 20 Mar 2013
8016-8-54.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-54.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4439512
Filtered Sequences 0
Sequence length 1-100
%GC 42

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGTGTCGCTGGTGCGGTCTCACTGCAGCCTGCAGAGCCACA 15964 0.35958907195205236 No Hit
TGTGTCGCTGGTGCGGTCTCACGAGACTGCCCTTAACCTACACA 15846 0.35693112215937245 No Hit
TGTGTAGGTTAAGGGCAGTCTCGTGAGACCGCACCAGCGACACA 15551 0.3502862476776727 No Hit
TGTGGCTCTGCAGGCTGCAGTGAGACCGCACCAGCGACACA 15546 0.35017362268645746 No Hit
TGTGTCGCTGGTGCGGTCTCAGCTTGGCGCAATCATGACACA 13834 0.3116108256943556 No Hit
TGTGTCATGATTGCGCCAAGCTGAGACCGCACCAGCGACACA 13589 0.3060922011248083 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10732 0.24173828114441406 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10630 0.23944073132362292 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 9083 0.2045945590416244 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 8942 0.20141853428935433 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 8718 0.1963729346829111 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 8629 0.19436820983927963 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 8537 0.19229591000091903 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 8501 0.1914850100641692 No Hit
TTAGCTTTGAAGTGGATCCTACCAGTTTTATCATCTTTTCACATAAAAGT 8045 0.18121361086533835 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7841 0.17661851122375613 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7777 0.17517691133620092 No Hit
CATG 7733 0.1741858114135067 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 7223 0.16269806230955114 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 7132 0.16064828746943358 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 7036 0.15848588763810076 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT 6895 0.1553098628858307 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6822 0.15366553801408805 No Hit
* 6744 0.15190858815113012 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6738 0.15177343816167183 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6703 0.15098506322316507 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6693 0.15075981324073456 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 6575 0.14810186344805465 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6493 0.14625481359212453 No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG 6490 0.14618723859739538 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6486 0.1460971386044232 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6424 0.1447005887133541 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 6404 0.14425008874849307 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 6348 0.14298868884688226 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6279 0.1414344639681118 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6268 0.14118668898743827 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 6038 0.1360059393915367 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6014 0.1354653394337035 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 6000 0.13514998945830084 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 5964 0.13433908952155102 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 5887 0.13260466465683615 No Hit
TAACTAACTCATAAATGCCTATTTTTTCTTACAGAAAAGGGATTTATAAA 5860 0.1319964897042738 No Hit
AACCTGACAGAAAAACAAAACTCTTCATATTGGTCAATTTCATAATCTTC 5855 0.13188386471305855 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 5787 0.13035216483253115 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 5762 0.12978903987645488 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 5740 0.1292934899151078 No Hit
GACTGAACAAGTAGGAAAATGACAGAGTTATCTTCCCAATTGATGTAAGT 5729 0.12904571493443423 No Hit
CATGACTATAAATATCAAGCAAATATGATTTTACTCACAAGTTCTGGAAT 5706 0.1285276399748441 No Hit
CATGGAGGAAGTAGGCAGCTGACAGAAAGTGCTGCAATTGCCTGTGTCAA 5705 0.12850511497660103 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATCTGGGTTTTTTTC 5664 0.12758159004863598 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 5623 0.12665806512067093 No Hit
ATCATGTCCCCAGAAAATCCAGTTACCTCTTGGTCAGTCATCCGGAAGCA 5557 0.1251714152366296 No Hit
TAATAGGGTGGTTCTCTTCCCAAAGTGGAAGCCAAATTCATCAATTATGT 5520 0.12433799030163675 No Hit
CAATTCAAATAAAAACGAAACTGTGTCAATTAGTTGAAGTAATGATGGCA 5348 0.12046369060383212 No Hit
ACTTGAAAGCAAGGATAGAGAAACATTTTAGAGATATCTGGGTTTTTTTC 5195 0.11701736587264547 No Hit
CATGACTATAAATATGAAGCAAATATGATTATCCTCACAAGTTCTGGAAT 5187 0.11683716588670105 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 5163 0.11629656592886785 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 5151 0.11602626594995125 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5124 0.11541809099738891 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 5101 0.11490001603779874 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 5095 0.11476486604834045 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 5087 0.11458466606239605 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 5055 0.11386386611861843 No Hit
CATGTCCTCCTTTCTACCAATAACCGCATATCTACTCTTTTTCTGTACTT 5044 0.1136160911379449 No Hit
GTATCTTAATGATGTATATAATTGCCTTCAATCCCCTTCTCACCCCACCC 5021 0.11309801617835474 No Hit
CATGTTGTCAGATTACCCTTTAGAATGCCTGTTGCTTTTAAAATATTTTT 5020 0.11307549118011169 No Hit
CATGCTTTACTCAACTGAAATAATCAAGCCCCTATTGCTGTGGGGGAAGC 4990 0.11239974123282018 No Hit
CAGCATAATAAAAAATAGGATTCCCAGCTTTGGAAGCCCCAGCTTGGTAG 4987 0.11233216623809103 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 4830 0.10879574151393216 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 4779 0.1076469666035366 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 4740 0.10676849167205765 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 4675 0.10530436678625939 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 4623 0.10413306687762078 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 4617 0.10399791688816247 No Hit
TAAAACATCGCCTACAGAAAAGCGTATGAAAGGAGTGTAAGCTTTGCTCT 4582 0.10320954194965573 No Hit
GGGATCATAAAGCTGTTGGAAGATGACCTTTTGATAAATGGCAACACTTC 4519 0.10179046706034356 No Hit
CCAGAATTCCTTCTCTGCTGGAGGCACACTCCCCCAAGGGCACAAAGGAA 4508 0.10154269207967002 No Hit
ATGTCAGATATAAAGAACTCACCGAACAGCAGCTCCCAGGCGCACTTCCT 4484 0.10100209212183682 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 4463 0.10052906715873276 No Hit
TAAGAAATTGCCACACACACAGCCATCCCAGCAACCTTCAGCAACTACCA 4445 0.10012361719035785 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2666080 3.2250345 6.753706 2
TTTTT 2360705 3.1484742 4.5320535 60-64
GGGGG 486830 2.9088223 7.629401 4
CCAGC 688625 2.9017153 8.206417 30-34
GGAGG 613230 2.6621196 5.1627345 4
GCTGG 578225 2.5343688 43.35907 7
CCTGG 583095 2.530472 5.3599315 95-96
CCCCA 598240 2.495955 5.249864 8
CCTCC 577530 2.4570575 7.966377 6
TGGGG 512825 2.2701411 9.37374 3
GGCAG 528110 2.269958 5.5737324 8
GGTGG 497065 2.2003758 6.3419676 7
CACCA 710530 2.1752977 5.7983 30-34
TGCAG 681305 2.1695952 8.164469 9
TGGTG 660530 2.1663063 31.164373 9
TGTGT 882760 2.1449327 34.5145 1
CTGCA 679875 2.1436582 8.098877 8
ACACA 935580 2.1018038 5.608966 35-39
GAGGG 475245 2.0631068 7.4271955 9
TGTGG 615435 2.0184107 9.311629 1
CTCTG 620255 1.9942341 8.299854 6
CAGTG 618945 1.9710116 5.6858954 5
TCTGC 610880 1.9640919 8.808519 7
TGGCT 602640 1.9569267 11.122981 3
GAGGA 615215 1.9404182 5.5586343 1
AAGAA 1129180 1.8800095 5.8196177 2
CCCAC 449500 1.8753874 5.5191417 9
ACCAG 605840 1.8732897 6.593963 30-34
TCCTC 563480 1.7937984 5.3345375 5
TGTTT 988560 1.7795811 5.5914693 3
CTGGT 547315 1.7772723 30.654898 8
GCCCC 308465 1.7713442 5.68236 9
GTGGC 401725 1.7607666 12.070391 2
GGCTC 395670 1.717099 14.036558 4
AGGGG 375650 1.6307507 5.4767127 6
GAAAG 680930 1.5603973 6.5184183 5
ATGGA 652745 1.5253015 10.926724 2
GAGAC 477730 1.4919018 6.063377 20-24
AGCAA 650715 1.4764297 5.655663 8
TCTCA 631055 1.4741354 5.305163 15-19
GTGTC 451520 1.4662013 39.007835 2
TGCCC 340735 1.4640915 7.7035522 3
GGTGC 333360 1.4611218 8.747734 10-14
AAGCA 638075 1.4477502 5.248114 7
GCAGT 452950 1.4424057 6.5832877 4
AAAGC 633065 1.4363829 5.049269 6
CATGG 448425 1.4279958 39.344658 1
GCTCT 443075 1.4245678 7.91966 5
GTCTC 440180 1.41526 7.763884 15-19
TAAAA 1139010 1.4049736 8.73514 1
GACAC 431380 1.33385 7.0307813 35-39
ATGTG 559605 1.3334391 7.0889997 2
TGTCA 562980 1.3282317 6.876364 3
GGTCT 388405 1.2612506 6.9676785 15-19
TGGGT 377620 1.2384609 6.0709167 3
ATGAA 718895 1.2205114 7.9318924 2
CATGA 517380 1.1970465 29.743784 1
ATGCC 375270 1.1832331 8.249087 2
ATGGC 371035 1.1815499 7.8039265 2
CATGT 492975 1.1630697 34.801235 1
GGAGT 358770 1.1538888 5.4348106 7
GTCTT 477110 1.1478333 5.166012 9
TGACT 485820 1.1461891 5.174853 3
ATGGG 354225 1.1392711 12.336497 2
CAGCG 267595 1.1388348 5.7715044 30-34
AACAC 505190 1.1349219 5.6321144 2
TGGTT 458790 1.1147692 5.2560043 9
CGCTG 254250 1.1033751 39.530434 6
GCAAG 347880 1.0863936 5.508162 9
ATGAC 461725 1.0682793 8.745052 2
TGTAG 439290 1.0467498 12.431839 3
ATGTT 591660 1.0444977 8.600706 2
ATGCT 438735 1.035102 7.140368 2
ATGAT 593655 1.027756 5.952138 2
ATGGT 429640 1.0237557 6.637334 2
TAAAG 592755 1.0063559 7.174143 1
GTAGG 310890 0.9998955 12.403058 4
GTCAT 418790 0.988046 6.581318 4
ATGTA 546840 0.9467081 9.96815 2
AGGTT 391405 0.9326484 6.8886833 6
CATGC 294035 0.9270976 29.81755 1
GTGCG 207170 0.90802914 8.603235 10-14
TCATG 384655 0.9075117 5.5918293 5
TGCGG 205155 0.8991974 8.889057 10-14
CGCAC 209965 0.8847467 5.859941 25-29
CCGCA 207750 0.8754131 7.361423 25-29
GCGGT 196145 0.8597064 8.82038 10-14
CGGTC 196320 0.8519748 8.435721 10-14
GACCG 192970 0.8212447 6.198112 25-29
ATGCA 338420 0.7829922 9.237907 2
ACCGC 185025 0.7796549 7.270132 25-29
TAAGA 454080 0.77091897 5.1949897 1
TAAGT 434230 0.7517538 6.3209586 1
TAACT 436565 0.7483315 6.5370293 1
TAATG 421545 0.7297931 5.230222 1
GATTG 305345 0.72758275 5.69859 9
GTGTA 299960 0.7147512 7.825644 2
TAAGG 298185 0.69678354 5.884708 1
TAGGT 291875 0.6954861 7.9299073 5
GTCGC 160120 0.6948768 39.171425 4
GCGAC 160040 0.68110067 5.752637 35-39
TAAAC 399475 0.67151433 5.799158 1
TCGCT 208785 0.6712823 29.05667 5
CGACA 196465 0.6074803 5.7471185 35-39
TGTCG 186185 0.6045905 29.479935 3
GGTTA 245180 0.58422023 7.141956 7
TGCCG 114795 0.49817875 6.829053 5
GCCGT 96155 0.41728628 5.706313 6
CCGTC 92795 0.3987274 5.656829 7
Produced by FastQC (version 0.10.0)