##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 8016-8-55.h.sapiens.aligned.bam File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12306976 Filtered Sequences 0 Sequence length 1-100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.993728597504376 24.0 24.0 31.0 23.0 31.0 2 31.02943407765932 31.0 31.0 32.0 29.0 33.0 3 32.6914667814643 33.0 33.0 34.0 29.0 35.0 4 33.89702895899479 34.0 33.0 36.0 29.0 37.0 5 35.44510651837967 36.0 35.0 37.0 33.0 37.0 6 35.758539363763 36.0 36.0 38.0 33.0 38.0 7 35.77805391106506 37.0 35.0 37.0 34.0 37.0 8 35.625181634563496 36.0 36.0 37.0 32.0 38.0 9 35.65220876291405 37.0 36.0 37.0 31.0 38.0 10-14 36.02057486233395 37.0 36.0 37.4 32.4 38.0 15-19 37.01563804778333 38.0 37.2 38.4 33.4 39.4 20-24 36.65235242450601 37.4 36.0 38.8 32.8 39.8 25-29 36.31161671794221 37.6 36.2 38.0 32.8 38.2 30-34 36.17396414506909 37.6 35.6 38.2 32.6 38.6 35-39 36.21213865676939 37.8 36.4 38.0 31.6 38.6 40-44 36.13441242262942 37.4 36.0 38.4 32.4 38.8 45-49 36.0800127259726 37.6 35.8 38.2 32.4 38.6 50-54 36.315973482440164 37.6 36.2 38.4 33.4 39.0 55-59 36.02830400446739 37.6 35.8 38.2 32.4 38.6 60-64 36.03352470767411 37.6 35.6 38.2 32.6 38.6 65-69 35.847799343977286 37.4 36.0 38.2 31.6 38.6 70-74 35.90578666242743 37.4 36.0 38.4 32.0 39.0 75-79 35.62287314643692 37.4 35.6 37.6 32.2 38.4 80-84 35.73625319723484 37.2 35.8 38.2 31.6 38.6 85-89 35.45007347139942 37.2 35.4 37.6 31.0 38.2 90-94 35.44379654904189 37.0 35.0 38.2 32.8 38.4 95-99 35.292552830782874 37.4 35.2 37.6 31.2 38.2 100 34.20978645515984 36.0 33.0 38.0 28.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 1.0 2 11.0 3 44.0 4 0.0 5 7.0 6 84.0 7 249.0 8 394.0 9 5066.0 10 972.0 11 1266.0 12 1932.0 13 2924.0 14 4353.0 15 5417.0 16 6746.0 17 8241.0 18 10680.0 19 14008.0 20 18057.0 21 20440.0 22 22096.0 23 25140.0 24 31417.0 25 41184.0 26 55748.0 27 76722.0 28 105278.0 29 140141.0 30 182197.0 31 238675.0 32 311977.0 33 426738.0 34 635893.0 35 1154685.0 36 3576163.0 37 5182014.0 38 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.610164053487054 19.30050257468372 22.798615937216997 38.290717434612226 2 17.72351702665572 51.69133343630348 15.716664092412996 14.868485444627805 3 19.745685027030994 35.450576325503135 29.41199996195737 15.3917386855085 4 32.86601108394217 25.369136180731356 21.48201735128852 20.282835384037956 5 21.243327144277096 27.52173418884204 26.543482279890274 24.69145638699059 6 21.644954137988005 29.153102434049373 26.279596315428655 22.92234711253397 7 21.990027292168985 27.757557140285456 27.303796169469358 22.948619398076197 8 21.30586000244741 27.209200310764174 28.531045723069443 22.953893963718976 9 21.460122359184204 29.259884106535083 27.291279109051203 21.98871442522951 10-14 21.953611310565805 27.463013391744205 27.461938466739333 23.12143683095066 15-19 21.209767565588074 27.714834240365562 28.117512145291496 22.957886048754876 20-24 21.436451942313568 27.021208596328293 28.280735480122924 23.26160398123522 25-29 21.448879306920183 27.48946938600073 28.832108017919627 22.22954328915946 30-34 21.805905021445053 28.104904195939522 27.899789090673522 22.189401691941907 35-39 21.931926072489468 27.304201459423 28.818050712386285 21.94582175570125 40-44 21.653384151549464 28.01495579188285 28.173441763557626 22.15821829301006 45-49 21.927452711332123 28.545790045175423 28.17814673693484 21.348610506557613 50-54 21.89347201411234 28.03596628595416 28.171386427355134 21.899175272578365 55-59 21.81793399567033 28.639175507158033 27.88291888069811 21.659971616473527 60-64 21.790805342893552 28.211198628426782 28.7412891551639 21.256706873515764 65-69 21.83660822249093 28.73162629389957 28.478523592489736 20.953241891119763 70-74 21.691802237783904 28.52584728974167 28.049372652425962 21.732977820048465 75-79 21.946276711389466 28.572750492208037 28.021795029347444 21.459177767055053 80-84 21.409044441592087 28.601886276518062 28.36081551709666 21.62825376479319 85-89 21.78899025787227 28.569630193219798 27.986550630899128 21.654828918008803 90-94 21.659156866934488 28.179062040950996 28.307299957825805 21.85448113428871 95-99 21.615469705488486 27.67126481409361 28.53398649166413 22.17927898875377 100 22.237695677342565 27.39825162475646 27.665708040269294 22.698344657631683 >>END_MODULE >>Per base GC content fail #Base %GC 1 57.90088148809927 2 32.59200247128352 3 35.137423712539494 4 53.14884646798013 5 45.93478353126768 6 44.56730125052197 7 44.93864669024518 8 44.25975396616638 9 43.448836784413714 10-14 45.07504814151646 15-19 44.16765361434295 20-24 44.698055923548786 25-29 43.67842259607964 30-34 43.99530671338696 35-39 43.877747828190714 40-44 43.81160244455952 45-49 43.276063217889735 50-54 43.79264728669071 55-59 43.47790561214386 60-64 43.04751221640932 65-69 42.7898501136107 70-74 43.424780057832365 75-79 43.40545447844452 80-84 43.03729820638528 85-89 43.443819175881075 90-94 43.51363800122321 95-99 43.79474869424225 100 44.93604033497425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4677.0 1 4677.5 2 4676.5 3 4675.5 4 4684.0 5 4700.0 6 4716.5 7 4744.0 8 4773.5 9 4808.0 10 4864.5 11 4921.0 12 4978.0 13 5092.5 14 5201.0 15 5785.5 16 6042.0 17 6108.0 18 7277.0 19 8354.5 20 11285.0 21 14422.0 22 21900.0 23 31061.0 24 49746.0 25 79086.5 26 109815.5 27 138553.5 28 155760.0 29 198299.5 30 257387.0 31 281183.5 32 301029.5 33 344826.5 34 402653.0 35 421647.0 36 390842.5 37 377277.0 38 430649.5 39 480286.0 40 509168.0 41 513375.0 42 478804.0 43 490239.5 44 521081.5 45 495090.0 46 429408.0 47 367276.0 48 353579.0 49 380469.0 50 405373.5 51 409287.0 52 334361.0 53 246450.5 54 225627.5 55 205769.5 56 162364.0 57 138764.5 58 165152.0 59 171672.0 60 151121.5 61 152048.5 62 138825.5 63 116700.5 64 91296.0 65 84320.5 66 102948.5 67 95326.5 68 60496.0 69 43953.0 70 38318.0 71 34311.5 72 31463.0 73 19948.0 74 12708.5 75 15040.5 76 14987.5 77 13059.5 78 10899.0 79 10237.5 80 8627.5 81 8122.5 82 6557.0 83 4805.5 84 5012.5 85 4057.0 86 3678.5 87 2753.0 88 2491.5 89 2653.0 90 2730.0 91 2851.0 92 2476.5 93 2150.5 94 2125.5 95 2105.0 96 2087.5 97 2081.5 98 2071.5 99 2068.0 100 2066.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055399474249401316 2 0.00789285165316225 3 0.0019427375268935025 4 0.0073158128286679724 5 0.00434933147523389 6 7.723829045158301E-4 7 3.658890278092736E-4 8 1.463565393280755E-4 9 6.504743543574504E-5 10-14 0.00137087648524832 15-19 0.017833036883081774 20-24 0.015983002479488317 25-29 0.00840847988277293 30-34 0.007837380844698675 35-39 0.005518224923206197 40-44 0.012818584337634964 45-49 0.011663647392980902 50-54 0.010478006732437681 55-59 0.011111086128035458 60-64 0.05422915403428981 65-69 0.04395830480825026 70-74 0.027669514463609074 75-79 0.07488567530960182 80-84 0.0642398471405481 85-89 0.01526886425284561 90-94 0.007564534917264217 95-99 0.009410260328548631 100 0.008270263477320046 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-1 4705.0 2-3 152.0 4-5 2520.0 6-7 866.0 8-9 18.0 10-11 16.0 12-13 6.0 14-15 25.0 16-17 159.0 18-19 612.0 20-21 4514.0 22-23 1408.0 24-25 6317.0 26-27 975.0 28-29 919.0 30-31 2001.0 32-33 960.0 34-35 1132.0 36-37 655.0 38-39 625.0 40-41 972.0 42-43 807.0 44-45 1294.0 46-47 2848.0 48-49 871.0 50-51 27751.0 52-53 2242.0 54-55 32125.0 56-57 37599.0 58-59 65578.0 60-61 3427.0 62-63 29300.0 64-65 19532.0 66-67 17282.0 68-69 20305.0 70-71 49049.0 72-73 46448.0 74-75 21889.0 76-77 27435.0 78-79 10760.0 80-81 21394.0 82-83 27689.0 84-85 11444.0 86-87 5540.0 88-89 7991.0 90-91 31014.0 92-93 7427.0 94-95 11523.0 96-97 286192.0 98-99 0.0 100-101 1.1450663E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 97.32674091309656 #Duplication Level Relative count 1 100.0 2 8.106346295608098 3 2.6629219643847133 4 1.5072223609901123 5 1.2233235654277064 6 1.0149686553797603 7 0.9046989515239761 8 0.7627495537427731 9 0.6457783761719964 10++ 5.016966912996595 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 13293 0.10801191129323727 No Hit CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 13225 0.1074593791358657 No Hit GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 13122 0.10662245542690585 No Hit CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 12732 0.10345352099492192 No Hit ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 12674 0.10298224356657558 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position AAAAA 6528555 3.1129637 6.047213 2 TTTTT 6075950 3.0730708 4.2387924 75-79 GGGGG 1482365 2.6290154 7.1054316 4 CCCCA 1968995 2.5378308 6.250884 1 CCAGC 1874605 2.450523 5.2493744 5 CCTCC 1870075 2.4389186 5.9475155 6 TGGGG 1707090 2.355692 9.241428 3 CTGCC 1770815 2.3422995 5.156597 7 GAGGG 1533480 2.0913172 5.129274 8 TGGCT 1825260 1.932324 5.291963 3 TGCAG 1792320 1.8752124 7.3820763 3 GCCCA 1384060 1.8092722 5.9888515 2 GGCTC 1311740 1.759738 7.252563 1 TGCCC 1180735 1.5617867 8.131325 3 GCTCC 1171730 1.5498754 6.486392 2 GGCCC 899405 1.5289755 6.634688 1 CATGG 1441390 1.5080523 38.332424 1 TAAAA 3069500 1.4809653 7.8966537 1 ATGGA 1785415 1.4568298 10.120607 2 GCAGT 1315500 1.3763402 5.4161787 4 ATGTG 1583490 1.3073903 8.856091 2 TGGGT 1211820 1.3011407 5.5415783 3 ATGGC 1223220 1.2797923 9.633906 2 ATGAA 1985975 1.2460837 7.8146315 2 ATGGG 1173360 1.2450798 12.336829 2 ATGCC 1154980 1.1914572 7.8162017 2 CATGT 1340625 1.0913557 29.025532 1 ATGCT 1306095 1.063246 7.791496 2 CATGA 1320245 1.062168 24.363287 1 ATGTT 1625490 1.0442351 6.686327 2 ATGGT 1259205 1.0396482 6.470949 2 TAAAG 1608845 1.0094566 5.4037213 1 CATGC 940135 0.9698269 29.70644 1 ATGAT 1517840 0.9636509 5.0574293 2 ATGAC 1189195 0.95673525 5.831439 2 ATGTA 1434530 0.91075873 6.545614 2 ATGCA 1040880 0.8374125 9.906194 2 TAAGA 1303060 0.81759435 5.011645 1 TAATG 1208700 0.7673831 5.3097763 1 TAAGT 1171185 0.7435655 6.9323664 1 TAAAC 1100775 0.6809905 5.2067094 1 TAAGG 828045 0.6756528 5.999074 1 TGCCG 385105 0.51662976 7.5480857 5 >>END_MODULE