WTSI - Auditory Brainstem Response (ABR) Measurements for Atp5a1

Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
ABR Thresholds are determined and plotted as a function of stimulus. Mean Threshold is plotted (+/- population standard deviation). The green shaded area indicates the median and 95% reference range (2.5 percentile to 97.5 percentile) of a large population of wildtype mice of this genetic background.
Click-Evoked ABRs waveforms recorded at 20dB and 50dB above threshold are plotted overlaid for individual mice and a mean (+/- population standard deviation) amplitude plotted for mutants and controls.
Amplitudes, Latencies and Inter-Peak Intervals for click-evoked waves P1, N1, P3 and N3 were measured and are plotted as a function of dB relative to threshold. Means are plotted (+/- population standard deviation) over a median & 95% reference range of wildtype data.

Mutant

Mouse ID	Datafile	Sex	Genotype	Age (wks)	Click	6kHz	12kHz	18kHz	24kHz	30kHz
MBCK40.3b	M00366776	F	Atp5a1/+	14.4	25	35	15	10	35	35
MBCK40.3c	M00366777	F	Atp5a1/+	14.4	25	30	15	20	30	35
MBCK47.1h	M00371902	F	Atp5a1/+	13.7	30	55	35	30	35	50
MBCK47.1i	M00371903	F	Atp5a1/+	13.7	25	30	10	25	30	40
MBCK51.1b	M00426554	F	Atp5a1/+	14.2	25	30	15	20	25	35
MBCK53.1c	M00426566	F	Atp5a1/+	13.9	25	30	10	20	35	45
MBCK53.1d	M00426567	F	Atp5a1/+	13.9	25	30	15	20	25	35
MBCK54.2a	M00493006	M	Atp5a1/+	13.8	25	30	10	20	25	30
MBCK54.2b	M00493007	M	Atp5a1/+	13.8	25	40	25	25	25	35
MBCK61.1a	M00528793	M	Atp5a1/+	14.1	25	40	15	30	35	35
MBCK61.1b	M00528794	M	Atp5a1/+	14.1	25	35	15	15	30	40
Mean				14.04	25.45	35.00	16.36	21.36	30.00	37.73
Standard Deviation				0.27	1.51	7.75	7.45	5.95	4.47	4.64
Standard Error				0.08	0.45	2.23	2.25	1.80	1.35	1.70

Control

Mouse ID	Datafile	Sex	Genotype	Age (wks)	Click	6kHz	12kHz	18kHz	24kHz	30kHz
MAKT46.4e	M00363924	F	+/+	14	30	40	25	20	40	35
MAKT46.4g	M00368352	F	+/+	14	25	30	15	25	35	35
MAKT49.3d	M00363888	M	+/+	14.1	25	35	10	25	35	40
MAKT49.3f	M00363890	M	+/+	14.1	25	35	20	20	30	25
MBCL209.1b	M00532611	M	+/+	13.9	30	40	30	30	30	30
MBCL213.1b	M00532651	M	+/+	14.1	20	25	10	15	25	25
MBEQ54.1f	M00425103	F	+/+	14.1	20	30	10	20	25	35
MBEQ54.1h	M00425105	F	+/+	14.1	20	40	20	10	30	30
MBKW63.1a	M00485910	M	+/+	14.2	25	45	20	25	30	35
MBKW63.1b	M00485911	M	+/+	14.2	25	25	15	20	35	30
Mean				14.12	24.50	34.50	17.50	21.00	31.50	32.00
Standard Deviation				0.09	3.69	6.85	6.77	5.68	4.74	4.83
Standard Error				0.03	1.17	2.06	2.14	1.80	1.50	1.53