Auditory Brainstem Response (ABR) measurement for Spns2tm1a(KOMP)Wtsi
Results Summary
Spns2tm1a(KOMP)Wtsi homozygote mice show severe hearing impairment.
Methods Summary
Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
ABR Thresholds are determined and plotted as a function of stimulus. Mean Threshold is plotted (+/- population standard deviation). The green shaded area indicates the median and 95% reference range (2.5 percentile to 97.5 percentile) of a large population of wildtype mice of this genetic background.
Click-Evoked ABRs waveforms recorded at 20dB and 50dB above threshold are plotted overlaid for individual mice and a mean (+/- population standard deviation) amplitude plotted for mutants and controls.
Amplitudes, Latencies and Inter-Peak Intervals for click-evoked waves P1, N1, P3 and N3 were measured and are plotted as a function of dB relative to threshold. Means are plotted (+/- population standard deviation) over a median & 95% reference range of wildtype data.