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Names

At the left side of the editor window is the "names panel". This either displays an ASCII pictorial summary of the sequence layout or the actual sequence names themselves depending on the settings in use. Between the names panel and the sequences panel is a vertical line, visible at the right edge of the above image. This can be dragged left and right to adjust the proportion of display dedicated to the names and sequence panels.

The default name display looks like this:

[picture]

This plot is a mini diagram of the way the sequences overlap. Here the > and < symbols represent the start of sequences, assembled on either the forward or reverse strand, with the ... sections reflecting their relative lengths. The background shading indicates the mapping quality of the sequence (which may not be available in many cases, depending on how the assembly was derived). This should indicate the likelihood that the sequence has been assembled to the correct point. Sequence that appears to map elsewhere (eg due to a repeat) will be dark grey while unique sequence will be light grey or white. Moving the mouse cursor over a sequence will tell you the precise mapping quality along with additional information such as the sequence name, the technology used (Sanger, Illumina, 454, etc), and whether it is part of a pair of sequences.

In the editor Settings menu is a checkbox labelled "Pack Sequences". When checked we permit multiple sequences to be drawn in the same row. Unchecking this reverts to the Gap4 style of display where each sequence has its own dedicated row. This also has an affect on the names panel, which switches to showing the sequence names, as below.

[picture]

This still uses the > and < symbols to reflect strand and grey scales for representing the mapping quality. The > and < are now also coloured independently. A dark grey > or < indicates that the read is not paired, while light means it forms a pair. (In future this may be expanded to indicate read-pair consistency and pairs spanning contigs.)

At the bottom of the names panel is an editable text field containing the current "padded" display position. This is updated automatically as we scroll through the editor, or it can be used to jump the editor to specific points by typing in a new location and pressing the enter key.

In both display modes, pressing the right mouse button brings up a context sensitive menu containing operations relevant to that specific sequence. This may contain the following commands.

Copy to clipboard
This copies the sequence name to the clipboard for use in a subsequent paste operation. Note that there is no visual cue that this has happened. The same function may also be achieved by left-clicking and dragging the mouse horiztonally, as if attempting to highlight a region of text.
Goto...
This lists other sequences sharing the same template, such as the other end of a read-pair. Selecting this command will jump the editor to the left-most base in that sequence. If the sequence is in another contig then a new editor will be created, unless one already exists for that contig in which case that other editor will be moved accordingly.

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Last generated on 8 Febuary 2011.