The Display Menu

The buttons in this menu are (mostly) toggles controlling which parts of the Artemis display are visible. "Show Entry Buttons", "Show Base View" and "Show Feature List" default to on/active, while the others default to off/inactive. Some general information about the graphs can be found in the section called Graphs and Plots. Configuration options for graphs are described in the section called Options For Plots and Graphs in Chapter 4.

Show Entry Buttons

This toggle controls whether the entry button line is visible. (see the section called The Entry Button Line). [Default: on]

Show Base View

This toggle controls whether the base view is visible. (see the section called The Overview and DNA Views). [Default: on]

Show Feature List

This toggle controls whether the feature view is visible. (see the section called The Feature List). [Default: on]

Hide All Graphs

This item will turn off all the visible graphs.

Add Usage Plots ...

This menu item prompts the user for the name of a file which should contain codon usage data in the same format as the data at this web site. If Artemis successfully loads the codon usage file two new plots will be added to the display menu and will be immediately visible. One plot shows the codon scores (in a sliding window) for each of the forward reading frames and the other shows the same thing for the reverse reading frames. [short name: gc_content]

Here is an example usage file:

UUU 32.2( 48423)  UCU 30.5( 45913)  UAU 21.8( 32829)  UGU  8.9( 13371)
UUC 13.0( 19519)  UCC 12.1( 18149)  UAC 11.8( 17721)  UGC  5.6(  8372)
UUA 26.0( 39138)  UCA 17.9( 26850)  UAA  1.3(  1944)  UGA  0.5(   733)
UUG 24.0( 36134)  UCG  8.0( 12055)  UAG  0.5(   705)  UGG 10.9( 16364)

CUU 25.3( 38015)  CCU 21.9( 32964)  CAU 16.3( 24577)  CGU 16.3( 24495)
CUC  7.3( 10922)  CCC  8.4( 12619)  CAC  6.4(  9653)  CGC  6.2(  9316)
CUA  8.6( 12957)  CCA 12.7( 19075)  CAA 27.3( 41066)  CGA  7.9( 11896)
CUG  6.3(  9503)  CCG  4.6(  6910)  CAG 10.9( 16457)  CGG  3.0(  4487)

AUU 35.0( 52636)  ACU 22.9( 34419)  AAU 33.9( 51009)  AGU 14.7( 22108)
AUC 12.6( 19000)  ACC 10.9( 16378)  AAC 17.9( 26895)  AGC  9.2( 13905)
AUA 13.1( 19726)  ACA 13.9( 20898)  AAA 39.3( 59079)  AGA 11.1( 16742)
AUG 20.9( 31376)  ACG  6.5(  9744)  AAG 25.2( 37825)  AGG  5.1(  7615)

GUU 29.3( 44015)  GCU 30.2( 45397)  GAU 38.1( 57240)  GGU 22.0( 33101)
GUC 11.0( 16497)  GCC 11.6( 17518)  GAC 15.8( 23749)  GGC  8.5( 12717)
GUA 12.3( 18451)  GCA 15.7( 23649)  GAA 44.3( 66550)  GGA 15.7( 23623)
GUG  8.3( 12422)  GCG  5.3(  8011)  GAG 21.3( 31979)  GGG  4.3(  6497)
      

Add User Plot ...

Artemis is able to display some types of user data in a graph that looks like the GC content graph (see the section called GC Content (%)). This menu item will prompt the user for the name of a data file which should contain one line per base of sequence and one floating point number per line. Artemis will plot each data point over the corresponding base.

GC Content (%)

Controls whether the GC content plot is visible. This is a graph of the average GC content of a moving window (default size 120 base), across the bases visible in the overview window. [Default: off] [short name: gc_content]

GC Content (%) With 2.5 SD Cutoff

Controls whether the cutoff GC content plot is visible. This is similar to the GC content graph, but the plot is clipped so that the GC content of each algorithm window is shown only when it is more than 2.5 times the standard deviation of the GC content in all the windows. [Default: off] [short name: sd_gc_content]

AG Content (%)

Controls whether the AG content plot is visible. This is a graph of the average AG content of a moving window (default size 120 base), across the bases visible in the overview window. [Default: off] [short name: ag_content]

GC Frame Plot

Controls whether the GC frame plot is visible. This graph is similar to the GC content graph but shows the GC content of the first, second and third position independently. For more information on the algorithm and on how to interpret the result see this web page. [Default: off] [short name: gc_frame]

Correlation Scores

Controls whether the (forward) correlation scores plot is visible. The graph shows the correlation between the amino acid composition of the globular proteins in TREMBL and the composition of the base translation in each of the three reading frames. The green line represents forward frame 1, blue represents frame 2 and red represents frame 3. [Default: off] [short name: correlation_score]

Reverse Correlation Scores

This does the same as "Correlation Scores", but does the calculation on the reverse strand. The green line represents reverse frame 1 (the bottom frame line), blue represents frame 2 and red represents frame 3. [Default: off] [short name: correlation_score]

GC Deviation (G-C)/(G+C)

Controls whether the GC deviation plot is visible. This graph shows the difference between the "G" content of the forward strand and the reverse strand. [Default: off] [short name: gc_deviation]

AT Deviation (A-T)/(A+T)

Controls whether the AT deviation plot is visible. This graph shows the difference between the "A" content of the forward strand and the reverse strand. [Default: off] [short name: at_deviation]